Restriction map for
pFN21ASDB0042
(Circular) (Six-base) MAPSORT of: pFN21ASDB0042.seq Check: 3235 from: 1 to: 6742
pFN21ASDB0042 6742 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4552 278
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3771 4085 1937
Size: 1834 314 4594
Fragments arranged by size:
4594 1834 314
Acc65I G'GTAC_C
Cuts at: 2315 4063 2315
Size: 1748 4994
AclI AA'CG_TT
Cuts at: 1563 4869 5242 1563
Size: 3306 373 3063
Fragments arranged by size:
3306 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2811 3134 3645 4805 6189 6457 1632
Size: 1179 323 511 1160 1384 268 1917
Fragments arranged by size:
1917 1384 1179 1160 511 323 268
AfeI AGC'GCT
Cuts at: 3197 3197
Size: 6742
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6545
AflIII A'CryG_T
Cuts at: 1203 5641 6618 1203
Size: 4438 977 1327
Fragments arranged by size:
4438 1327 977
AgeI A'CCGG_T
Cuts at: 5573 5573
Size: 6742
AhdI GACnn_n'nnGTC
Cuts at: 5471 5471
Size: 6742
AleI CACnn'nnGTG
Cuts at: 1166 2196 1166
Size: 1030 5712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6742
AlwNI CAG_nnn'CTG
Cuts at: 3139 4027 4485 6057 3139
Size: 888 458 1572 3824
Fragments arranged by size:
3824 1572 888 458
ApaLI G'TGCA_C
Cuts at: 4799 5955 4799
Size: 1156 5586
ApoI r'AATT_y
Cuts at: 1493 4051 4277 4429 6538 1493
Size: 2558 226 152 2109 1697
Fragments arranged by size:
2558 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5296 160
Size: 872 4264 1606
Fragments arranged by size:
4264 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
AvaI C'yCGr_G
Cuts at: 1870 1943 4067 1870
Size: 73 2124 4545
Fragments arranged by size:
4545 2124 73
BamHI G'GATC_C
Cuts at: 4072 4439 4072
Size: 367 6375
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2217 2315 4063 5518 618
Size: 325 217 627 430 98 1748 1455 1842
Fragments arranged by size:
1842 1748 1455 627 430 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2559 3606 3840 4061 1391
Size: 1168 1047 234 221 4072
Fragments arranged by size:
4072 1168 1047 234 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2961 3221 3743 928
Size: 971 1062 260 522 3927
Fragments arranged by size:
3927 1062 971 522 260
BbvCI CC'TCA_GC
Cuts at: 3305 3305
Size: 6742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3353 3387 3478 3512 4933 4967 1903
Size: 34 1416 34 91 34 1421 34 3678
Fragments arranged by size:
3678 1421 1416 91 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4634 5844 3674
Size: 960 1210 4572
Fragments arranged by size:
4572 1210 960
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6742
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5353 136
Size: 107 122 71 631 605 3681 1525
Fragments arranged by size:
3681 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2851 3793 6737 2851
Size: 942 2944 2856
Fragments arranged by size:
2944 2856 942
BlpI GC'TnA_GC
Cuts at: 4159 4159
Size: 6742
Bme1580I G_kGCm'C
Cuts at: 1790 2215 4803 5959 1790
Size: 425 2588 1156 2573
Fragments arranged by size:
2588 2573 1156 425
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6742
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 2553 3897
Size: 416 53 83 399 24 959 177 1344
Cuts at: 3897 3939 4473 5431 442
Size: 42 534 958 1753
Fragments arranged by size:
1753 1344 959 958 534 416 399 177
83 53 42 24
BmtI G_CTAG'C
Cuts at: 1056 2996 1056
Size: 1940 4802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2688 2688
Size: 6742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2341 2644 2772 3590 5402 1239
Size: 935 167 303 128 818 1812 2579
Fragments arranged by size:
2579 1812 935 818 303 167 128
Bpu10I CC'TnA_GC
Cuts at: 3305 3305
Size: 6742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2712 4867 5732 6030 2712
Size: 2155 865 298 3424
Fragments arranged by size:
3424 2155 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5405 882
Size: 617 3906 2219
Fragments arranged by size:
3906 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4039 4533 493
Size: 711 2835 494 2702
Fragments arranged by size:
2835 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3050 6736 3050
Size: 3686 3056
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4549 4931 5566 275
Size: 53 83 186 936 3016 382 635 1451
Fragments arranged by size:
3016 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2106 3570 5175 5573 5847 5994 6405 2106
Size: 1464 1605 398 274 147 411 2443
Fragments arranged by size:
2443 1605 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2472 2502 1178
Size: 30 165 30 1069 30 5418
Fragments arranged by size:
5418 1069 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 3010 1186
Size: 886 938 4918
Fragments arranged by size:
4918 938 886
BseYI C'CCAG_C
Cuts at: 3541 3874 4027 5945 6441 3541
Size: 333 153 1918 496 3842
Fragments arranged by size:
3842 1918 496 333 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6169
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3036 4192 4953 5981 1540
Size: 275 186 1035 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3606 4803 4888 5959 3606
Size: 1197 85 1071 4389
Fragments arranged by size:
4389 1197 1071 85
BsmI GAATG_Cn'
Cuts at: 4253 4346 4253
Size: 93 6649
BsmBI CGTCTCn'nnnn_
Cuts at: 3293 3293
Size: 6742
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2215 2559 3606 3840 4061 4803 4888
Size: 399 425 344 1047 234 221 742 85
Cuts at: 4888 5959 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1047 742 425 399 344 234
221 85
BspEI T'CCGG_A
Cuts at: 2106 6405 2106
Size: 4299 2443
BspHI T'CATG_A
Cuts at: 4631 6361 4631
Size: 1730 5012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2461 4097 844
Size: 1617 1636 3489
Fragments arranged by size:
3489 1636 1617
BsrBI CCG'CTC
Cuts at: 1394 1823 2545 4629 1394
Size: 429 722 2084 3507
Fragments arranged by size:
3507 2084 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 3448 3856 5237 5411 65
Size: 1183 1069 1131 408 1381 174 1396
Fragments arranged by size:
1396 1381 1183 1131 1069 408 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5386 5573 1700
Size: 110 3576 187 2869
Fragments arranged by size:
3576 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6742
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6742
BssSI C'ACGA_G
Cuts at: 4802 5814 4802
Size: 1012 5730
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2334 3958 794
Size: 1540 1624 3578
Fragments arranged by size:
3578 1624 1540
BstBI TT'CG_AA
Cuts at: 2007 5546 2007
Size: 3539 3203
BstEII G'GTnAC_C
Cuts at: 1184 3566 1184
Size: 2382 4360
BstXI CCAn_nnnn'nTGG
Cuts at: 3978 3978
Size: 6742
BstYI r'GATC_y
Cuts at: 1972 2851 3315 3408 3793 4072 4439 4824 4841
Size: 879 464 93 385 279 367 385 17
Cuts at: 4841 6293 6379 6391 6737 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 879 464 385 385 367 346
279 93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2819 2819
Size: 6742
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2966 487
Size: 683 56 1740 4263
Fragments arranged by size:
4263 1740 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 4258 5052 5072 2463
Size: 1795 794 20 4133
Fragments arranged by size:
4133 1795 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6707
DraI TTT'AAA
Cuts at: 4046 4046
Size: 6742
DraIII CAC_nnn'GTG
Cuts at: 2281 2971 2281
Size: 690 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3671 5749 809
Size: 507 2355 2078 1802
Fragments arranged by size:
2355 2078 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2519 3843 4014
Size: 54 1425 50 275 18 689 1324 171
Cuts at: 4014 4189 5078 6707 8
Size: 175 889 1629 43
Fragments arranged by size:
1629 1425 1324 889 689 275 175 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4189 1537
Size: 275 2377 4090
Fragments arranged by size:
4090 2377 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2931 4672 1353
Size: 741 837 1741 3423
Fragments arranged by size:
3423 1741 837 741
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5329 5703 5849 387
Size: 1064 138 3740 374 146 1280
Fragments arranged by size:
3740 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3604 3604
Size: 6742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2341 2644 2772 2811 3134 3590
Size: 393 542 167 303 128 39 323 456
Cuts at: 3590 3645 4805 5402 6189 6457 1239
Size: 55 1160 597 787 268 1524
Fragments arranged by size:
1524 1160 787 597 542 456 393 323
303 268 167 128 55 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3398 3561 3398
Size: 163 6579
EcoO109I rG'GnC_Cy
Cuts at: 3342 3342
Size: 6742
EcoRI G'AATT_C
Cuts at: 4051 4429 4051
Size: 378 6364
FspI TGC'GCA
Cuts at: 5248 5248
Size: 6742
HaeII r_GCGC'y
Cuts at: 1678 3199 5889 1678
Size: 1521 2690 2531
Fragments arranged by size:
2690 2531 1521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2472 2504 2608 2640 2642
Size: 32 135 32 900 32 104 32 2
Cuts at: 2642 2674 2701 2733 3730 3762 5383 5415 5457
Size: 32 27 32 997 32 1621 32 42
Cuts at: 5457 5489 1373
Size: 32 2626
Fragments arranged by size:
2626 1621 997 900 135 104 42 32
32 32 32 32 32 32 32 32
27 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4086 4332 669
Size: 988 281 2148 246 3079
Fragments arranged by size:
3079 2148 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3428 748
Size: 2680 4062
HpaI GTT'AAC
Cuts at: 4332 4332
Size: 6742
KpnI G_GTAC'C
Cuts at: 2319 4067 2319
Size: 1748 4994
MfeI C'AATT_G
Cuts at: 4341 4341
Size: 6742
MluI A'CGCG_T
Cuts at: 6618 6618
Size: 6742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 3541 5856 6040 1305
Size: 702 1534 2315 184 2007
Fragments arranged by size:
2315 2007 1534 702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2521 3845 4016 10
Size: 54 1425 343 689 1324 171 2736
Fragments arranged by size:
2736 1425 1324 689 343 171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 2880 3086 4700 5059 5218 518
Size: 648 1030 684 206 1614 359 159 2042
Fragments arranged by size:
2042 1614 1030 684 648 359 206 159
MspA1I CmG'CkG
Cuts at: 1606 2642 3169 4158 4835 5983 6228 1606
Size: 1036 527 989 677 1148 245 2120
Fragments arranged by size:
2120 1148 1036 989 677 527 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6190
NdeI CA'TA_TG
Cuts at: 387 3208 3804 387
Size: 2821 596 3325
Fragments arranged by size:
3325 2821 596
NheI G'CTAG_C
Cuts at: 1052 2992 1052
Size: 1940 4802
NotI GC'GGCC_GC
Cuts at: 4189 4189
Size: 6742
NspI r_CATG'y
Cuts at: 1132 1229 3010 4100 5645 1132
Size: 97 1781 1090 1545 2229
Fragments arranged by size:
2229 1781 1545 1090 97
PasI CC'CwG_GG
Cuts at: 1348 3971 1348
Size: 2623 4119
PciI A'CATG_T
Cuts at: 5641 5641
Size: 6742
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3139 3169 1108
Size: 2031 30 4681
Fragments arranged by size:
4681 2031 30
PfoI T'CCnGG_A
Cuts at: 2660 3422 2660
Size: 762 5980
PmeI GTTT'AAAC
Cuts at: 4046 4046
Size: 6742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 6324 6356 4811
Size: 32 1481 32 5197
Fragments arranged by size:
5197 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3342 3342
Size: 6742
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6742
PsiI TTA'TAA
Cuts at: 4312 4312
Size: 6742
PstI C_TGCA'G
Cuts at: 830 2458 3351 3999 4094 830
Size: 1628 893 648 95 3478
Fragments arranged by size:
3478 1628 893 648 95
PvuI CG_AT'CG
Cuts at: 2001 3036 2001
Size: 1035 5707
PvuII CAG'CTG
Cuts at: 2642 3169 2642
Size: 527 6215
SacI G_AGCT'C
Cuts at: 3606 3606
Size: 6742
SalI G'TCGA_C
Cuts at: 1936 4084 1936
Size: 2148 4594
SbfI CC_TGCA'GG
Cuts at: 4094 4094
Size: 6742
ScaI AGT'ACT
Cuts at: 4990 6702 4990
Size: 1712 5030
SexAI A'CCwGG_T
Cuts at: 2277 2277
Size: 6742
SfcI C'TryA_G
Cuts at: 826 1045 2087 2194 2454 3055 3347 3797 3995
Size: 219 1042 107 260 601 292 450 198
Cuts at: 3995 4090 5225 5906 6097 826
Size: 95 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 601 450 292 260
219 198 191 107 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6742
SmaI CCC'GGG
Cuts at: 1872 4069 1872
Size: 2197 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2727 4846 5747 6009 820
Size: 197 926 784 2119 901 262 1553
Fragments arranged by size:
2119 1553 926 901 784 262 197
SnaBI TAC'GTA
Cuts at: 493 4039 4533 493
Size: 3546 494 2702
Fragments arranged by size:
3546 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6742
SphI G_CATG'C
Cuts at: 1132 3010 4100 1132
Size: 1878 1090 3774
Fragments arranged by size:
3774 1878 1090
SspI AAT'ATT
Cuts at: 5 52 4666 5
Size: 47 4614 2081
Fragments arranged by size:
4614 2081 47
StuI AGG'CCT
Cuts at: 1517 2824 2919 1517
Size: 1307 95 5340
Fragments arranged by size:
5340 1307 95
StyI C'CwwG_G
Cuts at: 513 1065 2340 2889 3244 3394 4181 513
Size: 552 1275 549 355 150 787 3074
Fragments arranged by size:
3074 1275 787 552 549 355 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 3866 4769 4786 4939 5124 5569
Size: 1255 918 1368 903 17 153 185 445
Cuts at: 5569 325
Size: 1498
Fragments arranged by size:
1498 1368 1255 918 903 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4988 6700
Size: 274 80 33 51 1443 855 2156 1712
Cuts at: 6700 96
Size: 138
Fragments arranged by size:
2156 1712 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3268 3300 6702 6734 3268
Size: 32 3402 32 3276
Fragments arranged by size:
3402 3276 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6742
XbaI T'CTAG_A
Cuts at: 4078 4078
Size: 6742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4024 4024
Size: 6742
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6742
XmaI C'CCGG_G
Cuts at: 1870 4067 1870
Size: 2197 4545
XmnI GAAnn'nnTTC
Cuts at: 4871 4871
Size: 6742
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4550 276
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI