(Circular) (Six-base) MAPSORT of: pFN21ASDB0041.seq Check: 8035 from: 1 to: 6118 pFN21ASDB0041 6118 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 2483 3308 3439 2483 Size: 825 131 5162 Fragments arranged by size: 5162 825 131 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2760 2802 3201 4181 5565 5833 1632 Size: 1128 42 399 980 1384 268 1917 Fragments arranged by size: 1917 1384 1128 980 399 268 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 2178 4847 2178 Size: 2669 3449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2439 1184 Size: 1255 4863 AlwNI CAG_nnn'CTG Cuts at: 2654 3861 5433 2654 Size: 1207 1572 3339 Fragments arranged by size: 3339 1572 1207 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2894 3427 3653 3805 5914 1493 Size: 1401 533 226 152 2109 1697 Fragments arranged by size: 2109 1697 1401 533 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2193 3324 3443 1870 Size: 73 250 1131 119 4545 Fragments arranged by size: 4545 1131 250 119 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2467 2500 2467 Size: 33 6085 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2483 3308 3439 4894 Size: 325 217 627 246 450 825 131 1455 Cuts at: 4894 618 Size: 1842 Fragments arranged by size: 1842 1455 825 627 450 325 246 217 131 BanII G_rGCy'C Cuts at: 1391 3437 1391 Size: 2046 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2169 928 Size: 971 270 4877 Fragments arranged by size: 4877 971 270 BbvCI CC'TCA_GC Cuts at: 2629 2629 Size: 6118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2521 3239 4010 5220 2521 Size: 718 771 1210 3419 Fragments arranged by size: 3419 1210 771 718 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2534 6113 2534 Size: 3579 2539 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2036 4179 5335 1790 Size: 246 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2631 2790 4778 1239 Size: 1392 159 1988 2579 Fragments arranged by size: 2579 1988 1392 159 Bpu10I CC'TnA_GC Cuts at: 2629 2629 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2271 2541 2551 3017 4243 5108 5406 2271 Size: 270 10 466 1226 865 298 2983 Fragments arranged by size: 2983 1226 865 466 298 270 10 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3160 4781 882 Size: 617 1661 1621 2219 Fragments arranged by size: 2219 1661 1621 617 BsaAI yAC'GTr Cuts at: 493 1204 3415 3909 493 Size: 711 2211 494 2702 Fragments arranged by size: 2702 2211 711 494 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2653 2683 2821 2851 1178 Size: 30 165 30 1250 30 138 30 4445 Fragments arranged by size: 4445 1250 165 138 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3273 3363 1186 Size: 2087 90 3941 Fragments arranged by size: 3941 2087 90 BseYI C'CCAG_C Cuts at: 2293 5321 5817 2293 Size: 3028 496 2594 Fragments arranged by size: 3028 2594 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 1163 Size: 573 903 4642 Fragments arranged by size: 4642 903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 2815 4179 4264 5335 2730 Size: 85 1364 85 1071 3513 Fragments arranged by size: 3513 1364 1071 85 85 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2730 2815 3437 4179 4264 5335 Size: 399 246 694 85 622 742 85 1071 Cuts at: 5335 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 694 622 399 246 85 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2650 3473 844 Size: 1806 823 3489 Fragments arranged by size: 3489 1806 823 BsrBI CCG'CTC Cuts at: 1394 1823 2028 4005 1394 Size: 429 205 1977 3507 Fragments arranged by size: 3507 1977 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 4613 4787 65 Size: 1183 3365 174 1396 Fragments arranged by size: 3365 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 2007 4922 2007 Size: 2915 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 2079 2534 3085 3448 3815 4200 4217 5669 Size: 107 455 551 363 367 385 17 1452 Cuts at: 5669 5755 5767 6113 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 551 455 385 367 363 346 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2224 2343 2604 3232 3238 3274 513 Size: 552 1159 119 261 628 6 36 3357 Fragments arranged by size: 3357 1159 628 552 261 119 36 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2622 3315 3634 4428 4448 2622 Size: 693 319 794 20 4292 Fragments arranged by size: 4292 794 693 319 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3385 3420 529 Size: 35 2821 35 3227 Fragments arranged by size: 3227 2821 35 35 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3235 3565 4454 Size: 54 1425 50 275 18 1405 330 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1405 889 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2940 4048 1353 Size: 1587 1108 3423 Fragments arranged by size: 3423 1587 1108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 4705 5079 5225 387 Size: 1064 138 501 2615 374 146 1280 Fragments arranged by size: 2615 1280 1064 501 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2631 2760 2790 2802 3201 4181 4778 Size: 393 999 129 30 12 399 980 597 Cuts at: 4778 5565 5833 1239 Size: 787 268 1524 Fragments arranged by size: 1524 999 980 787 597 399 393 268 129 30 12 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3011 3107 3011 Size: 96 6022 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2187 2196 2219 2519 Size: 32 135 32 592 23 9 23 300 Cuts at: 2519 2551 2653 2685 4759 4791 4833 4865 1373 Size: 32 102 32 2074 32 42 32 2626 Fragments arranged by size: 2626 2074 592 300 135 102 42 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 2487 3312 3443 2487 Size: 825 131 5162 Fragments arranged by size: 5162 825 131 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2850 4076 4435 4594 518 Size: 648 1684 1226 359 159 2042 Fragments arranged by size: 2042 1684 1226 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2654 3534 4211 5359 5604 1606 Size: 1048 880 677 1148 245 2120 Fragments arranged by size: 2120 1148 1048 880 677 245 NcoI C'CATG_G Cuts at: 513 1065 2343 2604 513 Size: 552 1278 261 4027 Fragments arranged by size: 4027 1278 552 261 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 3233 1108 Size: 1236 889 3993 Fragments arranged by size: 3993 1236 889 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspXI vC'TCGA_Gb Cuts at: 3324 3324 Size: 6118 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2654 2654 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 3466 4601 5282 5473 826 Size: 219 2421 1135 681 191 1471 Fragments arranged by size: 2421 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2193 2286 2556 2566 2996 3324 Size: 197 926 250 93 270 10 430 328 Cuts at: 3324 4222 5123 5385 820 Size: 898 901 262 1553 Fragments arranged by size: 1553 926 901 898 430 328 270 262 250 197 93 10 SnaBI TAC'GTA Cuts at: 493 3415 3909 493 Size: 2922 494 2702 Fragments arranged by size: 2922 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 3139 4042 5 Size: 47 3087 903 2081 Fragments arranged by size: 3087 2081 903 47 StuI AGG'CCT Cuts at: 1517 2686 1517 Size: 1169 4949 StyI C'CwwG_G Cuts at: 513 1065 2070 2343 2604 2825 3557 513 Size: 552 1005 273 261 221 732 3074 Fragments arranged by size: 3074 1005 732 552 273 261 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2185 4145 4162 4315 4500 4945 325 Size: 1255 605 1960 17 153 185 445 1498 Fragments arranged by size: 1960 1498 1255 605 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2743 4364 6076 Size: 274 80 33 51 1443 766 1621 1712 Cuts at: 6076 96 Size: 138 Fragments arranged by size: 1712 1621 1443 766 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2821 2853 6078 6110 2821 Size: 32 3225 32 2829 Fragments arranged by size: 3225 2829 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 2193 3324 1943 Size: 250 1131 4737 Fragments arranged by size: 4737 1131 250 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI