Restriction map for
pFN21ASDB0041
(Circular) (Six-base) MAPSORT of: pFN21ASDB0041.seq Check: 8035 from: 1 to: 6118
pFN21ASDB0041 6118 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 2483 3308 3439 2483
Size: 825 131 5162
Fragments arranged by size:
5162 825 131
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2760 2802 3201 4181 5565 5833 1632
Size: 1128 42 399 980 1384 268 1917
Fragments arranged by size:
1917 1384 1128 980 399 268 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 2178 4847 2178
Size: 2669 3449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2439 1184
Size: 1255 4863
AlwNI CAG_nnn'CTG
Cuts at: 2654 3861 5433 2654
Size: 1207 1572 3339
Fragments arranged by size:
3339 1572 1207
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2894 3427 3653 3805 5914 1493
Size: 1401 533 226 152 2109 1697
Fragments arranged by size:
2109 1697 1401 533 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 3324 3443 1870
Size: 73 250 1131 119 4545
Fragments arranged by size:
4545 1131 250 119 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2467 2500 2467
Size: 33 6085
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2483 3308 3439 4894
Size: 325 217 627 246 450 825 131 1455
Cuts at: 4894 618
Size: 1842
Fragments arranged by size:
1842 1455 825 627 450 325 246 217
131
BanII G_rGCy'C
Cuts at: 1391 3437 1391
Size: 2046 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2169 928
Size: 971 270 4877
Fragments arranged by size:
4877 971 270
BbvCI CC'TCA_GC
Cuts at: 2629 2629
Size: 6118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2521 3239 4010 5220 2521
Size: 718 771 1210 3419
Fragments arranged by size:
3419 1210 771 718
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2534 6113 2534
Size: 3579 2539
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2036 4179 5335 1790
Size: 246 2143 1156 2573
Fragments arranged by size:
2573 2143 1156 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2631 2790 4778 1239
Size: 1392 159 1988 2579
Fragments arranged by size:
2579 1988 1392 159
Bpu10I CC'TnA_GC
Cuts at: 2629 2629
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2271 2541 2551 3017 4243 5108 5406 2271
Size: 270 10 466 1226 865 298 2983
Fragments arranged by size:
2983 1226 865 466 298 270 10
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3160 4781 882
Size: 617 1661 1621 2219
Fragments arranged by size:
2219 1661 1621 617
BsaAI yAC'GTr
Cuts at: 493 1204 3415 3909 493
Size: 711 2211 494 2702
Fragments arranged by size:
2702 2211 711 494
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2653 2683 2821 2851 1178
Size: 30 165 30 1250 30 138 30 4445
Fragments arranged by size:
4445 1250 165 138 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3273 3363 1186
Size: 2087 90 3941
Fragments arranged by size:
3941 2087 90
BseYI C'CCAG_C
Cuts at: 2293 5321 5817 2293
Size: 3028 496 2594
Fragments arranged by size:
3028 2594 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 1163
Size: 573 903 4642
Fragments arranged by size:
4642 903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 2815 4179 4264 5335 2730
Size: 85 1364 85 1071 3513
Fragments arranged by size:
3513 1364 1071 85 85
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2730 2815 3437 4179 4264 5335
Size: 399 246 694 85 622 742 85 1071
Cuts at: 5335 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 694 622 399 246 85
85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2650 3473 844
Size: 1806 823 3489
Fragments arranged by size:
3489 1806 823
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 4005 1394
Size: 429 205 1977 3507
Fragments arranged by size:
3507 1977 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4613 4787 65
Size: 1183 3365 174 1396
Fragments arranged by size:
3365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 2007 4922 2007
Size: 2915 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 2079 2534 3085 3448 3815 4200 4217 5669
Size: 107 455 551 363 367 385 17 1452
Cuts at: 5669 5755 5767 6113 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 551 455 385 367 363 346
107 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2224 2343 2604 3232 3238 3274 513
Size: 552 1159 119 261 628 6 36 3357
Fragments arranged by size:
3357 1159 628 552 261 119 36 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2622 3315 3634 4428 4448 2622
Size: 693 319 794 20 4292
Fragments arranged by size:
4292 794 693 319 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3385 3420 529
Size: 35 2821 35 3227
Fragments arranged by size:
3227 2821 35 35
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 3565 4454
Size: 54 1425 50 275 18 1405 330 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1405 889 330 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2940 4048 1353
Size: 1587 1108 3423
Fragments arranged by size:
3423 1587 1108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 4705 5079 5225 387
Size: 1064 138 501 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 501 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2631 2760 2790 2802 3201 4181 4778
Size: 393 999 129 30 12 399 980 597
Cuts at: 4778 5565 5833 1239
Size: 787 268 1524
Fragments arranged by size:
1524 999 980 787 597 399 393 268
129 30 12
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3011 3107 3011
Size: 96 6022
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2187 2196 2219 2519
Size: 32 135 32 592 23 9 23 300
Cuts at: 2519 2551 2653 2685 4759 4791 4833 4865 1373
Size: 32 102 32 2074 32 42 32 2626
Fragments arranged by size:
2626 2074 592 300 135 102 42 32
32 32 32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 2487 3312 3443 2487
Size: 825 131 5162
Fragments arranged by size:
5162 825 131
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2850 4076 4435 4594 518
Size: 648 1684 1226 359 159 2042
Fragments arranged by size:
2042 1684 1226 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2654 3534 4211 5359 5604 1606
Size: 1048 880 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 880 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2343 2604 513
Size: 552 1278 261 4027
Fragments arranged by size:
4027 1278 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 3233 1108
Size: 1236 889 3993
Fragments arranged by size:
3993 1236 889
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspXI vC'TCGA_Gb
Cuts at: 3324 3324
Size: 6118
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2654 2654
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 3466 4601 5282 5473 826
Size: 219 2421 1135 681 191 1471
Fragments arranged by size:
2421 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2193 2286 2556 2566 2996 3324
Size: 197 926 250 93 270 10 430 328
Cuts at: 3324 4222 5123 5385 820
Size: 898 901 262 1553
Fragments arranged by size:
1553 926 901 898 430 328 270 262
250 197 93 10
SnaBI TAC'GTA
Cuts at: 493 3415 3909 493
Size: 2922 494 2702
Fragments arranged by size:
2922 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 3139 4042 5
Size: 47 3087 903 2081
Fragments arranged by size:
3087 2081 903 47
StuI AGG'CCT
Cuts at: 1517 2686 1517
Size: 1169 4949
StyI C'CwwG_G
Cuts at: 513 1065 2070 2343 2604 2825 3557 513
Size: 552 1005 273 261 221 732 3074
Fragments arranged by size:
3074 1005 732 552 273 261 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 4145 4162 4315 4500 4945 325
Size: 1255 605 1960 17 153 185 445 1498
Fragments arranged by size:
1960 1498 1255 605 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2743 4364 6076
Size: 274 80 33 51 1443 766 1621 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
1712 1621 1443 766 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2821 2853 6078 6110 2821
Size: 32 3225 32 2829
Fragments arranged by size:
3225 2829 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 2193 3324 1943
Size: 250 1131 4737
Fragments arranged by size:
4737 1131 250
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI