Restriction map for
pFN21ASDB0033
(Circular) (Six-base) MAPSORT of: pFN21ASDB0033.seq Check: 8100 from: 1 to: 7918
pFN21ASDB0033 7918 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4543 4543
Size: 7918
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5728 278
Size: 53 83 186 936 4192 2468
Fragments arranged by size:
4192 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5261 1937
Size: 3324 4594
Acc65I G'GTAC_C
Cuts at: 2044 5239 2044
Size: 3195 4723
AclI AA'CG_TT
Cuts at: 1563 6045 6418 1563
Size: 4482 373 3063
Fragments arranged by size:
4482 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5981 7365 7633 1632
Size: 4349 1384 268 1917
Fragments arranged by size:
4349 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3731 820
Size: 197 2714 5007
Fragments arranged by size:
5007 2714 197
AflIII A'CryG_T
Cuts at: 1203 6817 7794 1203
Size: 5614 977 1327
Fragments arranged by size:
5614 1327 977
AgeI A'CCGG_T
Cuts at: 6749 6749
Size: 7918
AhdI GACnn_n'nnGTC
Cuts at: 6647 6647
Size: 7918
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2508 2518 2576 1184
Size: 1324 10 58 6526
Fragments arranged by size:
6526 1324 58 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 2588
Size: 32 7886
AlwNI CAG_nnn'CTG
Cuts at: 2088 2752 4231 4633 4843 5661 7233 2088
Size: 664 1479 402 210 818 1572 2773
Fragments arranged by size:
2773 1572 1479 818 664 402 210
ApaI G_GGCC'C
Cuts at: 5108 5108
Size: 7918
ApaLI G'TGCA_C
Cuts at: 2705 5975 7131 2705
Size: 3270 1156 3492
Fragments arranged by size:
3492 3270 1156
ApoI r'AATT_y
Cuts at: 1493 3644 4280 5203 5227 5453 5605 7714 1493
Size: 2151 636 923 24 226 152 2109 1697
Fragments arranged by size:
2151 2109 1697 923 636 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 6472 160
Size: 872 5440 1606
Fragments arranged by size:
5440 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 3272 3524 4430 4746 5243 1870
Size: 73 570 759 252 906 316 497 4545
Fragments arranged by size:
4545 906 759 570 497 316 252 73
BamHI G'GATC_C
Cuts at: 5248 5615 5248
Size: 367 7551
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2525 2651 4789 5120
Size: 325 217 627 257 481 126 2138 331
Cuts at: 5120 5175 5239 6694 618
Size: 55 64 1455 1842
Fragments arranged by size:
2138 1842 1455 627 481 331 325 257
217 126 64 55
BanII G_rGCy'C
Cuts at: 1391 3117 3125 3248 3279 3378 3432 3795 3882
Size: 1726 8 123 31 99 54 363 87
Cuts at: 3882 4769 4776 5108 5237 1391
Size: 887 7 332 129 4072
Fragments arranged by size:
4072 1726 887 363 332 129 123 99
87 54 31 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6947
BbvCI CC'TCA_GC
Cuts at: 3047 3047
Size: 7918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6109 6143 1903
Size: 34 4172 34 3678
Fragments arranged by size:
4172 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3559 5810 7020 3559
Size: 2251 1210 4457
Fragments arranged by size:
4457 2251 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6529 136
Size: 107 122 71 631 605 4857 1525
Fragments arranged by size:
4857 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2304 4164 4659 7913 2304
Size: 1860 495 3254 2309
Fragments arranged by size:
3254 2309 1860 495
BlpI GC'TnA_GC
Cuts at: 2936 5335 2936
Size: 2399 5519
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2656 2709 4061 5108 5178 5979 7135
Size: 571 295 53 1352 1047 70 801 1156
Cuts at: 7135 1790
Size: 2573
Fragments arranged by size:
2573 1352 1156 1047 801 571 295 70
53
BmgBI CAC'GTC
Cuts at: 1318 3238 4068 1318
Size: 1920 830 5168
Fragments arranged by size:
5168 1920 830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3823 4346 5649
Size: 416 53 83 399 24 2406 523 1303
Cuts at: 5649 6607 442
Size: 958 1753
Fragments arranged by size:
2406 1753 1303 958 523 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7918
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3179
Size: 7918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2585 2590 2774 3155 3572 3581 4232
Size: 965 381 5 184 381 417 9 651
Cuts at: 4232 4276 6578 1239
Size: 44 2302 2579
Fragments arranged by size:
2579 2302 965 651 417 381 381 184
44 9 5
Bpu10I CC'TnA_GC
Cuts at: 3047 3119 4937 3047
Size: 72 1818 6028
Fragments arranged by size:
6028 1818 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3368 6043 6908 7206 3011
Size: 357 2675 865 298 3723
Fragments arranged by size:
3723 2675 865 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2281 2492 3731 6581 882
Size: 617 782 211 1239 2850 2219
Fragments arranged by size:
2850 2219 1239 782 617 211
BsaAI yAC'GTr
Cuts at: 493 1204 4077 5017 5215 5709 493
Size: 711 2873 940 198 494 2702
Fragments arranged by size:
2873 2702 940 711 494 198
BsaBI GATnn'nnATC
Cuts at: 4169 7912 4169
Size: 3743 4175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2340 3159 4878 5725
Size: 53 83 186 936 807 819 1719 847
Cuts at: 5725 6107 6742 275
Size: 382 635 1451
Fragments arranged by size:
1719 1451 936 847 819 807 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2592 3723 3783 4916 6351 6749 7023 7170 7581
Size: 1131 60 1133 1435 398 274 147 411
Cuts at: 7581 2592
Size: 2929
Fragments arranged by size:
2929 1435 1133 1131 411 398 274 147
60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2514 2544 2588 2618 3464
Size: 30 165 30 1111 30 44 30 846
Cuts at: 3464 3494 3728 3758 4983 5013 1178
Size: 30 234 30 1225 30 4083
Fragments arranged by size:
4083 1225 1111 846 234 165 44 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 2783 2839 3005 3035 3125 3413 3488
Size: 1594 3 56 166 30 90 288 75
Cuts at: 3488 3524 1186
Size: 36 5580
Fragments arranged by size:
5580 1594 288 166 90 75 56 36
30 3
BseYI C'CCAG_C
Cuts at: 2506 2785 3248 7121 7617 2506
Size: 279 463 3873 496 2807
Fragments arranged by size:
3873 2807 496 463 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2654 3641 1163
Size: 573 450 468 987 5440
Fragments arranged by size:
5440 987 573 468 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3822 3928 4091 4810 5368 6129
Size: 275 186 1821 106 163 719 558 761
Cuts at: 6129 7157 1540
Size: 1028 2301
Fragments arranged by size:
2301 1821 1028 761 719 558 275 186
163 106
BsiHKAI G_wGCw'C
Cuts at: 2401 2709 3117 3263 3378 3795 5979 6064 7135
Size: 308 408 146 115 417 2184 85 1071
Cuts at: 7135 2401
Size: 3184
Fragments arranged by size:
3184 2184 1071 417 408 308 146 115
85
BsiWI C'GTAC_G
Cuts at: 3147 3147
Size: 7918
BsmI GAATG_Cn'
Cuts at: 5429 5522 5429
Size: 93 7825
BsmBI CGTCTCn'nnnn_
Cuts at: 3150 3809 4058 5018 3150
Size: 659 249 960 6050
Fragments arranged by size:
6050 960 659 249
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2401 2656 2709 3117 3125 3248
Size: 399 571 40 255 53 408 8 123
Cuts at: 3248 3263 3279 3378 3432 3795 3882 4061 4769
Size: 15 16 99 54 363 87 179 708
Cuts at: 4769 4776 5108 5178 5237 5979 6064 7135 1391
Size: 7 332 70 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 708 571 408 399 363
332 255 179 123 99 87 85 70
59 54 53 40 16 15 8 7
BspEI T'CCGG_A
Cuts at: 2592 3723 7581 2592
Size: 1131 3858 2929
Fragments arranged by size:
3858 2929 1131
BspHI T'CATG_A
Cuts at: 5807 7537 5807
Size: 1730 6188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2307 2510 2601 2940 3993 4543 5273
Size: 1385 78 203 91 339 1053 550 730
Cuts at: 5273 844
Size: 3489
Fragments arranged by size:
3489 1385 1053 730 550 339 203 91
78
BsrBI CCG'CTC
Cuts at: 1394 1823 2448 2725 5805 1394
Size: 429 625 277 3080 3507
Fragments arranged by size:
3507 3080 625 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 3004 6413 6587 65
Size: 1183 1756 3409 174 1396
Fragments arranged by size:
3409 1756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 2538 4001 6562 6749 1700
Size: 110 503 225 1463 2561 187 2869
Fragments arranged by size:
2869 2561 1463 503 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 3177 4963 96
Size: 3081 1786 3051
Fragments arranged by size:
3081 3051 1786
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7918
BssSI C'ACGA_G
Cuts at: 2697 3153 4516 5978 6990 2697
Size: 456 1363 1462 1012 3625
Fragments arranged by size:
3625 1462 1363 1012 456
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4633 4843 794
Size: 3839 210 3869
Fragments arranged by size:
3869 3839 210
BstBI TT'CG_AA
Cuts at: 2007 6722 2007
Size: 4715 3203
BstEII G'GTnAC_C
Cuts at: 1184 2122 3007 3673 1184
Size: 938 885 666 5429
Fragments arranged by size:
5429 938 885 666
BstXI CCAn_nnnn'nTGG
Cuts at: 2717 3238 4068 2717
Size: 521 830 6567
Fragments arranged by size:
6567 830 521
BstYI r'GATC_y
Cuts at: 1972 2304 3861 4164 4242 4659 5248 5615 6000
Size: 332 1557 303 78 417 589 367 385
Cuts at: 6000 6017 7469 7555 7567 7913 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1557 1452 589 417 385 367 346
332 303 86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 4481 4481
Size: 7918
BtgI C'CryG_G
Cuts at: 513 1065 2022 2127 2335 2376 2658 3264 4647
Size: 552 957 105 208 41 282 606 1383
Cuts at: 4647 5195 513
Size: 548 3236
Fragments arranged by size:
3236 1383 957 606 552 548 282 208
105 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3082 3403 487
Size: 683 56 1856 321 5002
Fragments arranged by size:
5002 1856 683 321 56
BtsI GCAGTG_nn'
Cuts at: 2113 2399 5434 6228 6248 2113
Size: 286 3035 794 20 3783
Fragments arranged by size:
3783 3035 794 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7883
DraI TTT'AAA
Cuts at: 5222 5222
Size: 7918
DraIII CAC_nnn'GTG
Cuts at: 3958 4518 3958
Size: 560 7358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2876 4883 6925 809
Size: 507 1560 2007 2042 1802
Fragments arranged by size:
2042 2007 1802 1560 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2665 2946 3809
Size: 54 1425 50 275 18 835 281 863
Cuts at: 3809 3925 4088 4233 5365 6254 7883 8
Size: 116 163 145 1132 889 1629 43
Fragments arranged by size:
1629 1425 1132 889 863 835 281 275
163 145 116 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3925 4088 5365 1537
Size: 275 2113 163 1277 4090
Fragments arranged by size:
4090 2113 1277 275 163
EarI CTCTTCn'nnn_
Cuts at: 1353 2781 3371 3725 4134 4289 4712 5848 1353
Size: 1428 590 354 409 155 423 1136 3423
Fragments arranged by size:
3423 1428 1136 590 423 409 354 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 3271 3638 3938 6505 6879
Size: 1064 138 807 875 367 300 2567 374
Cuts at: 6879 7025 387
Size: 146 1280
Fragments arranged by size:
2567 1280 1064 875 807 374 367 300
146 138
EcoICRI GAG'CTC
Cuts at: 3115 3376 3793 3115
Size: 261 417 7240
Fragments arranged by size:
7240 417 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2585 2590 2774 3155 3572 3581
Size: 393 572 381 5 184 381 417 9
Cuts at: 3581 4232 4276 5981 6578 7365 7633 1239
Size: 651 44 1705 597 787 268 1524
Fragments arranged by size:
1705 1524 787 651 597 572 417 393
381 381 268 184 44 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 3137 3167 4544 2825
Size: 312 30 1377 6199
Fragments arranged by size:
6199 1377 312 30
EcoO109I rG'GnC_Cy
Cuts at: 2085 2296 2929 5104 5105 2085
Size: 211 633 2175 1 4898
Fragments arranged by size:
4898 2175 633 211 1
EcoRI G'AATT_C
Cuts at: 3644 5203 5227 5605 3644
Size: 1559 24 378 5957
Fragments arranged by size:
5957 1559 378 24
FspI TGC'GCA
Cuts at: 2405 3995 4460 6424 2405
Size: 1590 465 1964 3899
Fragments arranged by size:
3899 1964 1590 465
FspAI rTGC'GCAy
Cuts at: 4460 4460
Size: 7918
HaeII r_GCGC'y
Cuts at: 1678 2030 7065 1678
Size: 352 5035 2531
Fragments arranged by size:
5035 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4290 4322 4389 4421 4930
Size: 32 135 32 2718 32 67 32 509
Cuts at: 4930 4962 6559 6591 6633 6665 1373
Size: 32 1597 32 42 32 2626
Fragments arranged by size:
2718 2626 1597 509 135 67 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5262 5508 669
Size: 988 281 3324 246 3079
Fragments arranged by size:
3324 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2093 4029 748
Size: 1345 1936 4637
Fragments arranged by size:
4637 1936 1345
HpaI GTT'AAC
Cuts at: 5508 5508
Size: 7918
KpnI G_GTAC'C
Cuts at: 2048 5243 2048
Size: 3195 4723
MfeI C'AATT_G
Cuts at: 5517 5517
Size: 7918
MluI A'CGCG_T
Cuts at: 7794 7794
Size: 7918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3369 3957 4141 7032 7216 1305
Size: 2064 588 184 2891 184 2007
Fragments arranged by size:
2891 2064 2007 588 184 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2667 2948 4235 10
Size: 54 1425 343 835 281 1287 3693
Fragments arranged by size:
3693 1425 1287 835 343 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2630 5876 6235 6394 518
Size: 648 900 564 3246 359 159 2042
Fragments arranged by size:
3246 2042 900 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2749 3127 3252 3616 3853 3894 4425 4492
Size: 1143 378 125 364 237 41 531 67
Cuts at: 4492 4633 4840 5334 6011 7159 7404 1606
Size: 141 207 494 677 1148 245 2120
Fragments arranged by size:
2120 1148 1143 677 531 494 378 364
245 237 207 141 125 67 41
NaeI GCC'GGC
Cuts at: 2315 4003 2315
Size: 1688 6230
NcoI C'CATG_G
Cuts at: 513 1065 2022 2127 513
Size: 552 957 105 6304
Fragments arranged by size:
6304 957 552 105
NdeI CA'TA_TG
Cuts at: 387 2322 387
Size: 1935 5983
NgoMIV G'CCGG_C
Cuts at: 2313 4001 2313
Size: 1688 6230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7918
NotI GC'GGCC_GC
Cuts at: 5365 5365
Size: 7918
NspI r_CATG'y
Cuts at: 1132 1229 5276 6821 1132
Size: 97 4047 1545 2229
Fragments arranged by size:
4047 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2101 1348
Size: 753 7165
PciI A'CATG_T
Cuts at: 6817 6817
Size: 7918
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2752 1108
Size: 1644 6274
PfoI T'CCnGG_A
Cuts at: 2685 3279 3549 4446 4506 2685
Size: 594 270 897 60 6097
Fragments arranged by size:
6097 897 594 270 60
PmeI GTTT'AAAC
Cuts at: 5222 5222
Size: 7918
PmlI CAC'GTG
Cuts at: 4077 5017 4077
Size: 940 6978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2588 2620 5987 6019 7500 7532 2588
Size: 32 3367 32 1481 32 2974
Fragments arranged by size:
3367 2974 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2929 2296
Size: 633 7285
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7918
PsiI TTA'TAA
Cuts at: 5488 5488
Size: 7918
PspOMI G'GGCC_C
Cuts at: 5104 5104
Size: 7918
PstI C_TGCA'G
Cuts at: 830 2304 2487 3360 3852 3870 4638 4758 5007
Size: 1474 183 873 492 18 768 120 249
Cuts at: 5007 5270 830
Size: 263 3478
Fragments arranged by size:
3478 1474 873 768 492 263 249 183
120 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7918
PvuII CAG'CTG
Cuts at: 2749 3252 3853 4492 4633 4840 2749
Size: 503 601 639 141 207 5827
Fragments arranged by size:
5827 639 601 503 207 141
SacI G_AGCT'C
Cuts at: 3117 3378 3795 3117
Size: 261 417 7240
Fragments arranged by size:
7240 417 261
SalI G'TCGA_C
Cuts at: 1936 5260 1936
Size: 3324 4594
SapI GCTCTTCn'nnn_
Cuts at: 3371 4712 3371
Size: 1341 6577
SbfI CC_TGCA'GG
Cuts at: 5270 5270
Size: 7918
ScaI AGT'ACT
Cuts at: 6166 7878 6166
Size: 1712 6206
SexAI A'CCwGG_T
Cuts at: 2820 3954 2820
Size: 1134 6784
SfcI C'TryA_G
Cuts at: 826 1045 2300 2308 2483 2546 3356 3679 3848
Size: 219 1255 8 175 63 810 323 169
Cuts at: 3848 3866 4634 4754 5003 5266 6401 7082 7273
Size: 18 768 120 249 263 1135 681 191
Cuts at: 7273 826
Size: 1471
Fragments arranged by size:
1471 1255 1135 810 768 681 323 263
249 219 191 175 169 120 63 18
8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7918
SmaI CCC'GGG
Cuts at: 1872 3526 5245 1872
Size: 1654 1719 4545
Fragments arranged by size:
4545 1719 1654
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3026 3383 3731 6022 6923 7185
Size: 197 926 1083 357 348 2291 901 262
Cuts at: 7185 820
Size: 1553
Fragments arranged by size:
2291 1553 1083 926 901 357 348 262
197
SnaBI TAC'GTA
Cuts at: 493 5215 5709 493
Size: 4722 494 2702
Fragments arranged by size:
4722 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7918
SphI G_CATG'C
Cuts at: 1132 5276 1132
Size: 4144 3774
SspI AAT'ATT
Cuts at: 5 52 5842 5
Size: 47 5790 2081
Fragments arranged by size:
5790 2081 47
StuI AGG'CCT
Cuts at: 1517 2570 4550 4760 1517
Size: 1053 1980 210 4675
Fragments arranged by size:
4675 1980 1053 210
StyI C'CwwG_G
Cuts at: 513 1065 2022 2127 3102 3168 3231 4761 5160
Size: 552 957 105 975 66 63 1530 399
Cuts at: 5160 5357 513
Size: 197 3074
Fragments arranged by size:
3074 1530 975 957 552 399 197 105
66 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 2651 3842 5945 5962 6115 6300
Size: 1255 897 174 1191 2103 17 153 185
Cuts at: 6300 6745 325
Size: 445 1498
Fragments arranged by size:
2103 1498 1255 1191 897 445 185 174
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 3177 4143
Size: 274 80 33 51 1443 645 555 966
Cuts at: 4143 4963 6164 7876 96
Size: 820 1201 1712 138
Fragments arranged by size:
1712 1443 1201 966 820 645 555 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 3497 3529 3728 3760 4365 4397 4502
Size: 32 951 32 199 32 605 32 105
Cuts at: 4502 4534 4983 5015 7878 7910 2514
Size: 32 449 32 2863 32 2522
Fragments arranged by size:
2863 2522 951 605 449 199 105 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7918
XbaI T'CTAG_A
Cuts at: 5254 5254
Size: 7918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3628 3628
Size: 7918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7918
XmaI C'CCGG_G
Cuts at: 1870 3524 5243 1870
Size: 1654 1719 4545
Fragments arranged by size:
4545 1719 1654
XmnI GAAnn'nnTTC
Cuts at: 3303 4439 6047 3303
Size: 1136 1608 5174
Fragments arranged by size:
5174 1608 1136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5726 276
Size: 53 83 186 936 4192 2468
Fragments arranged by size:
4192 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BstZ17I EcoRV
FalI FseI KasI NarI NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI