Restriction map for
pFN21ASDB0032
(Circular) (Six-base) MAPSORT of: pFN21ASDB0032.seq Check: 1355 from: 1 to: 6166
pFN21ASDB0032 6166 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2944 2944
Size: 6166
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2938 3976 278
Size: 53 83 186 936 1402 1038 2468
Fragments arranged by size:
2468 1402 1038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3459 3509 1937
Size: 1522 50 4594
Fragments arranged by size:
4594 1522 50
Acc65I G'GTAC_C
Cuts at: 3487 3487
Size: 6166
AclI AA'CG_TT
Cuts at: 1563 4293 4666 1563
Size: 2730 373 3063
Fragments arranged by size:
3063 2730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 3053 3265 3378 4229 5613 5881 1632
Size: 965 456 212 113 851 1384 268 1917
Fragments arranged by size:
1917 1384 965 851 456 268 212 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5969
AflIII A'CryG_T
Cuts at: 1203 5065 6042 1203
Size: 3862 977 1327
Fragments arranged by size:
3862 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 4895 4895
Size: 6166
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2136 1184
Size: 952 5214
AlwNI CAG_nnn'CTG
Cuts at: 3909 5481 3909
Size: 1572 4594
ApaLI G'TGCA_C
Cuts at: 4223 5379 4223
Size: 1156 5010
ApoI r'AATT_y
Cuts at: 1493 2393 2868 2942 3159 3475 3701 3853 5962
Size: 900 475 74 217 316 226 152 2109
Cuts at: 5962 1493
Size: 1697
Fragments arranged by size:
2109 1697 900 475 316 226 217 152
74
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 2649 3491 1870
Size: 73 706 842 4545
Fragments arranged by size:
4545 842 706 73
BamHI G'GATC_C
Cuts at: 3496 3863 3496
Size: 367 5799
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3487 4942 618
Size: 325 217 627 1700 1455 1842
Fragments arranged by size:
1842 1700 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3485 1391
Size: 2094 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3092 928
Size: 971 1193 4002
Fragments arranged by size:
4002 1193 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4357 4391 1903
Size: 34 2420 34 3678
Fragments arranged by size:
3678 2420 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 5268 4058
Size: 1210 4956
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6166
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4777 136
Size: 107 122 71 631 605 3105 1525
Fragments arranged by size:
3105 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2358 2486 6161 2358
Size: 128 3675 2363
Fragments arranged by size:
3675 2363 128
BlpI GC'TnA_GC
Cuts at: 2109 3583 2109
Size: 1474 4692
Bme1580I G_kGCm'C
Cuts at: 1790 4227 5383 1790
Size: 2437 1156 2573
Fragments arranged by size:
2573 2437 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2342 3897 4855
Size: 416 53 83 399 24 925 1555 958
Cuts at: 4855 442
Size: 1753
Fragments arranged by size:
1753 1555 958 925 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3387 4826 1239
Size: 2148 1439 2579
Fragments arranged by size:
2579 2148 1439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 4291 5156 5454 3101
Size: 1190 865 298 3813
Fragments arranged by size:
3813 1190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4829 882
Size: 617 3330 2219
Fragments arranged by size:
3330 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3463 3957 493
Size: 711 2259 494 2702
Fragments arranged by size:
2702 2259 711 494
BsaBI GATnn'nnATC
Cuts at: 3183 6160 3183
Size: 2977 3189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 3973 4355 4990
Size: 53 83 186 936 1402 1038 382 635
Cuts at: 4990 275
Size: 1451
Fragments arranged by size:
1451 1402 1038 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4599 4997 5271 5418 5829 4599
Size: 398 274 147 411 4936
Fragments arranged by size:
4936 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5941
Fragments arranged by size:
5941 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2481 1186
Size: 1295 4871
BseYI C'CCAG_C
Cuts at: 5369 5865 5369
Size: 496 5670
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 1163
Size: 573 336 5257
Fragments arranged by size:
5257 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3616 4377 5405 1540
Size: 275 186 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4227 4312 5383 4227
Size: 85 1071 5010
Fragments arranged by size:
5010 1071 85
BsmI GAATG_Cn'
Cuts at: 2931 3677 3770 2931
Size: 746 93 5327
Fragments arranged by size:
5327 746 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3485 4227 4312 5383 1391
Size: 399 1695 742 85 1071 2174
Fragments arranged by size:
2174 1695 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 4055 5785 4055
Size: 1730 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2944 3521 844
Size: 2100 577 3489
Fragments arranged by size:
3489 2100 577
BsrBI CCG'CTC
Cuts at: 1394 1823 4053 1394
Size: 429 2230 3507
Fragments arranged by size:
3507 2230 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2341 2448 2974 3036 4661 4835 65
Size: 1183 1093 107 526 62 1625 174 1396
Fragments arranged by size:
1625 1396 1183 1093 526 174 107 62
BsrFI r'CCGG_y
Cuts at: 1700 1810 4810 4997 1700
Size: 110 3000 187 2869
Fragments arranged by size:
3000 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2325 96
Size: 2229 3937
BssHII G'CGCG_C
Cuts at: 1925 2713 1925
Size: 788 5378
BssSI C'ACGA_G
Cuts at: 4226 5238 4226
Size: 1012 5154
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6166
BstBI TT'CG_AA
Cuts at: 2007 4970 2007
Size: 2963 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstYI r'GATC_y
Cuts at: 1972 2275 2358 2486 3496 3863 4248 4265 5717
Size: 303 83 128 1010 367 385 17 1452
Cuts at: 5717 5803 5815 6161 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1010 385 367 346 303 128
86 83 17 12
BstZ17I GTA'TAC
Cuts at: 3460 3460
Size: 6166
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5614
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 2459 3682 4476 4496 2459
Size: 1223 794 20 4129
Fragments arranged by size:
4129 1223 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 2791 3372 3470 2791
Size: 581 98 5487
Fragments arranged by size:
5487 581 98
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2833 3613 4502
Size: 54 1425 50 275 18 1003 780 889
Cuts at: 4502 6131 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1003 889 780 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3613 1537
Size: 275 1801 4090
Fragments arranged by size:
4090 1801 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2763 4096 1353
Size: 1410 1333 3423
Fragments arranged by size:
3423 1410 1333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4753 5127 5273 387
Size: 1064 138 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2597 3053 3265 3378 3387 4229 4826
Size: 393 965 456 212 113 9 842 597
Cuts at: 4826 5613 5881 1239
Size: 787 268 1524
Fragments arranged by size:
1524 965 842 787 597 456 393 268
212 113 9
EcoO109I rG'GnC_Cy
Cuts at: 2990 2990
Size: 6166
EcoRI G'AATT_C
Cuts at: 3159 3475 3853 3159
Size: 316 378 5472
Fragments arranged by size:
5472 378 316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3179 3179
Size: 6166
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 5313 1678
Size: 3635 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4807 4839 4881 4913 1373
Size: 32 135 32 3235 32 42 32 2626
Fragments arranged by size:
3235 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 3510 3756 669
Size: 988 281 250 1322 246 3079
Fragments arranged by size:
3079 1322 988 281 250 246
HindIII A'AGCT_T
Cuts at: 748 2130 2638 748
Size: 1382 508 4276
Fragments arranged by size:
4276 1382 508
HpaI GTT'AAC
Cuts at: 2188 3756 2188
Size: 1568 4598
KpnI G_GTAC'C
Cuts at: 3491 3491
Size: 6166
MfeI C'AATT_G
Cuts at: 3315 3765 3315
Size: 450 5716
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2237 5280 5464 1305
Size: 932 3043 184 2007
Fragments arranged by size:
3043 2007 932 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2835 10
Size: 54 1425 343 1003 3341
Fragments arranged by size:
3341 1425 1003 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4124 4483 4642 518
Size: 648 2958 359 159 2042
Fragments arranged by size:
2958 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 3356 3582 4259 5407 5652 1606
Size: 450 1300 226 677 1148 245 2120
Fragments arranged by size:
2120 1300 1148 677 450 245 226
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5614
NdeI CA'TA_TG
Cuts at: 387 2631 387
Size: 2244 3922
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6166
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 2326 3524 5069 1132
Size: 97 1097 1198 1545 2229
Fragments arranged by size:
2229 1545 1198 1097 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6166
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2831 3122 1108
Size: 1723 291 4152
Fragments arranged by size:
4152 1723 291
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2990 2990
Size: 6166
PshAI GACnn'nnGTC
Cuts at: 1613 2726 1613
Size: 1113 5053
PsiI TTA'TAA
Cuts at: 3051 3736 3051
Size: 685 5481
PspXI vC'TCGA_Gb
Cuts at: 2649 2649
Size: 6166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3334 3366 3334
Size: 32 6134
PstI C_TGCA'G
Cuts at: 830 2466 2677 3025 3518 830
Size: 1636 211 348 493 3478
Fragments arranged by size:
3478 1636 493 348 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2056 3356 2056
Size: 1300 4866
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SfcI C'TryA_G
Cuts at: 826 1045 2263 2379 2462 2673 2979 3021 3351
Size: 219 1218 116 83 211 306 42 330
Cuts at: 3351 3514 4649 5330 5521 826
Size: 163 1135 681 191 1471
Fragments arranged by size:
1471 1218 1135 681 330 306 219 211
191 163 116 83 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 3493 1872
Size: 1621 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2649 3080 4270 5171 5433 820
Size: 197 926 706 431 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 706 431 262 197
SnaBI TAC'GTA
Cuts at: 493 3463 3957 493
Size: 2970 494 2702
Fragments arranged by size:
2970 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3524 1132
Size: 2392 3774
SspI AAT'ATT
Cuts at: 5 52 3431 4090 5
Size: 47 3379 659 2081
Fragments arranged by size:
3379 2081 659 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6166
StyI C'CwwG_G
Cuts at: 513 1065 2836 3605 513
Size: 552 1771 769 3074
Fragments arranged by size:
3074 1771 769 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 3262 4193 4210 4363 4548 4993
Size: 1255 608 1074 931 17 153 185 445
Cuts at: 4993 325
Size: 1498
Fragments arranged by size:
1498 1255 1074 931 608 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 3406 4412
Size: 274 80 33 51 1443 348 1081 1006
Cuts at: 4412 6124 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1081 1006 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 6126 6158 2502
Size: 32 3592 32 2510
Fragments arranged by size:
3592 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6166
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 2649 1943
Size: 706 5460
XmaI C'CCGG_G
Cuts at: 1870 3491 1870
Size: 1621 4545
XmnI GAAnn'nnTTC
Cuts at: 2475 3346 4295 2475
Size: 871 949 4346
Fragments arranged by size:
4346 949 871
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2936 3974 276
Size: 53 83 186 936 1402 1038 2468
Fragments arranged by size:
2468 1402 1038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI