Restriction map for
pFN21ASDB0025
(Circular) (Six-base) MAPSORT of: pFN21ASDB0025.seq Check: 6983 from: 1 to: 6421
pFN21ASDB0025 6421 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2122 2122
Size: 6421
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2106 4231 278
Size: 53 83 186 936 570 2125 2468
Fragments arranged by size:
2468 2125 936 570 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 3435 3764 1937
Size: 169 1329 329 4594
Fragments arranged by size:
4594 1329 329 169
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2721 3604 3651 4484 5868 6136 1632
Size: 1089 883 47 833 1384 268 1917
Fragments arranged by size:
1917 1384 1089 883 833 268 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 2763 5320 6297 1203
Size: 1560 2557 977 1327
Fragments arranged by size:
2557 1560 1327 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 2742 5150 2742
Size: 2408 4013
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 3431 4164 5736 3431
Size: 733 1572 4116
Fragments arranged by size:
4116 1572 733
ApaLI G'TGCA_C
Cuts at: 3521 4478 5634 3521
Size: 957 1156 4308
Fragments arranged by size:
4308 1156 957
ApoI r'AATT_y
Cuts at: 1493 3730 3956 4108 6217 1493
Size: 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 3746 1870
Size: 73 1803 4545
Fragments arranged by size:
4545 1803 73
BamHI G'GATC_C
Cuts at: 2049 3675 3751 4118 2049
Size: 1626 76 367 4352
Fragments arranged by size:
4352 1626 367 76
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2434 2911 3392 3742
Size: 325 217 627 513 134 477 481 350
Cuts at: 3742 5197 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 513 481 477 350 325
217 134
BanII G_rGCy'C
Cuts at: 1391 2148 3740 1391
Size: 757 1592 4072
Fragments arranged by size:
4072 1592 757
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3045 3367 3467 928
Size: 971 1146 322 100 3882
Fragments arranged by size:
3882 1146 971 322 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3195 4313 5523 3195
Size: 1118 1210 4093
Fragments arranged by size:
4093 1210 1118
BclI T'GATC_A
Cuts at: 1548 2310 1548
Size: 762 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3630 5032 136
Size: 107 122 71 631 605 1958 1402 1525
Fragments arranged by size:
1958 1525 1402 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 3168 3838 3168
Size: 670 5751
Bme1580I G_kGCm'C
Cuts at: 1790 2437 3395 3525 4482 5638 1790
Size: 647 958 130 957 1156 2573
Fragments arranged by size:
2573 1156 958 957 647 130
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4152 5110 442
Size: 416 53 83 399 24 2735 958 1753
Fragments arranged by size:
2735 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5081 1239
Size: 3842 2579
Bpu10I CC'TnA_GC
Cuts at: 2628 2628
Size: 6421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4546 5411 5709 4546
Size: 865 298 5258
Fragments arranged by size:
5258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2333 2825 5084 882
Size: 617 834 492 2259 2219
Fragments arranged by size:
2259 2219 834 617 492
BsaAI yAC'GTr
Cuts at: 493 1204 2253 2416 3718 4212 493
Size: 711 1049 163 1302 494 2702
Fragments arranged by size:
2702 1302 1049 711 494 163
BsaBI GATnn'nnATC
Cuts at: 2054 6415 2054
Size: 4361 2060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 3372 4228 4610
Size: 53 83 186 936 570 1269 856 382
Cuts at: 4610 5245 275
Size: 635 1451
Fragments arranged by size:
1451 1269 936 856 635 570 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4854 5252 5526 5673 6084 4854
Size: 398 274 147 411 5191
Fragments arranged by size:
5191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2055 2085 2899 2929 1178
Size: 30 165 30 652 30 814 30 4670
Fragments arranged by size:
4670 814 652 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 3120 3300 3492 3675 1186
Size: 1516 418 180 192 183 3932
Fragments arranged by size:
3932 1516 418 192 183 180
BseYI C'CCAG_C
Cuts at: 5624 6120 5624
Size: 496 5925
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 2826 3095 1163
Size: 573 335 755 269 4489
Fragments arranged by size:
4489 755 573 335 269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3871 4632 5660 1540
Size: 275 186 1870 761 1028 2301
Fragments arranged by size:
2301 1870 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3525 4482 4567 5638 2148
Size: 1377 957 85 1071 2931
Fragments arranged by size:
2931 1377 1071 957 85
BsmI GAATG_Cn'
Cuts at: 2251 3932 4025 2251
Size: 1681 93 4647
Fragments arranged by size:
4647 1681 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2437 3395 3525 3740 4482 4567
Size: 399 358 289 958 130 215 742 85
Cuts at: 4567 5638 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 958 742 399 358 289 215
130 85
BspEI T'CCGG_A
Cuts at: 6084 6084
Size: 6421
BspHI T'CATG_A
Cuts at: 2148 2241 2502 2952 4310 6040 2148
Size: 93 261 450 1358 1730 2529
Fragments arranged by size:
2529 1730 1358 450 261 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 3265 3776 844
Size: 1278 1143 511 3489
Fragments arranged by size:
3489 1278 1143 511
BsrBI CCG'CTC
Cuts at: 1394 1823 4308 1394
Size: 429 2485 3507
Fragments arranged by size:
3507 2485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2724 4916 5090 65
Size: 1183 1476 2192 174 1396
Fragments arranged by size:
2192 1476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3000 3395 5065 5252 1700
Size: 110 1190 395 1670 187 2869
Fragments arranged by size:
2869 1670 1190 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 2520 96
Size: 2424 3997
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 2093 4481 5493 2093
Size: 2388 1012 3021
Fragments arranged by size:
3021 2388 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6421
BstBI TT'CG_AA
Cuts at: 2007 5225 2007
Size: 3218 3203
BstEII G'GTnAC_C
Cuts at: 1184 3334 1184
Size: 2150 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 2257
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 2049 3051 3675 3751 4118 4503 4520 5972
Size: 77 1002 624 76 367 385 17 1452
Cuts at: 5972 6058 6070 6416 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1002 624 385 367 346 86
77 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 3413 3413
Size: 6421
BtgI C'CryG_G
Cuts at: 513 1065 2272 3319 513
Size: 552 1207 1047 3615
Fragments arranged by size:
3615 1207 1047 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5682
Fragments arranged by size:
5682 683 56
BtsI GCAGTG_nn'
Cuts at: 2838 3937 4731 4751 2838
Size: 1099 794 20 4508
Fragments arranged by size:
4508 1099 794 20
ClaI AT'CG_AT
Cuts at: 1554 3642 1554
Size: 2088 4333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3468 3725 3468
Size: 257 6164
DraIII CAC_nnn'GTG
Cuts at: 2430 3325 2430
Size: 895 5526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2390 5428 809
Size: 507 1074 3038 1802
Fragments arranged by size:
3038 1802 1074 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 2379 2616
Size: 54 1425 50 275 18 487 62 237
Cuts at: 2616 2964 3144 3868 4757 6386 8
Size: 348 180 724 889 1629 43
Fragments arranged by size:
1629 1425 889 724 487 348 275 237
180 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4351 1353
Size: 2998 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2843 5008 5382 5528 387
Size: 1064 138 1254 2165 374 146 1280
Fragments arranged by size:
2165 1280 1254 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 6421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2721 3604 3651 4484 5081 5868 6136
Size: 393 1089 883 47 833 597 787 268
Cuts at: 6136 1239
Size: 1524
Fragments arranged by size:
1524 1089 883 833 787 597 393 268
47
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 3690 2057
Size: 1633 4788
EcoO109I rG'GnC_Cy
Cuts at: 2515 3238 3417 3601 2515
Size: 723 179 184 5335
Fragments arranged by size:
5335 723 184 179
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 6421
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 5568 1678
Size: 3890 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2665 2697 2728 2760 3667
Size: 32 135 32 1093 32 31 32 907
Cuts at: 3667 3699 5062 5094 5136 5168 1373
Size: 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 1093 907 135 42 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 2761 3765 4011 669
Size: 988 281 547 276 1004 246 3079
Fragments arranged by size:
3079 1004 988 547 281 276 246
HindIII A'AGCT_T
Cuts at: 748 3170 748
Size: 2422 3999
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2275 3094 3183 5535 5719 1305
Size: 970 819 89 2352 184 2007
Fragments arranged by size:
2352 2007 970 819 184 89
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2966 10
Size: 54 1425 343 1134 3465
Fragments arranged by size:
3465 1425 1134 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2306 4379 4738 4897 518
Size: 648 1089 51 2073 359 159 2042
Fragments arranged by size:
2073 2042 1089 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2798 3837 4514 5662 5907 1606
Size: 1192 1039 677 1148 245 2120
Fragments arranged by size:
2120 1192 1148 1039 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2272 513
Size: 552 1207 4662
Fragments arranged by size:
4662 1207 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 2527 2849 3313 3779 5324 1132
Size: 97 1298 322 464 466 1545 2229
Fragments arranged by size:
2229 1545 1298 466 464 322 97
PasI CC'CwG_GG
Cuts at: 1348 3527 1348
Size: 2179 4242
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 1108
Size: 1026 5395
PfoI T'CCnGG_A
Cuts at: 3208 3688 3208
Size: 480 5941
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PmlI CAC'GTG
Cuts at: 2253 2416 2253
Size: 163 6258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2515 3238 3417 3601 2515
Size: 723 179 184 5335
Fragments arranged by size:
5335 723 184 179
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PstI C_TGCA'G
Cuts at: 830 3773 830
Size: 2943 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6421
PvuII CAG'CTG
Cuts at: 2798 2798
Size: 6421
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SanDI GG'GwC_CC
Cuts at: 3238 3238
Size: 6421
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SexAI A'CCwGG_T
Cuts at: 2426 2754 2426
Size: 328 6093
SfcI C'TryA_G
Cuts at: 826 1045 3769 4904 5585 5776 826
Size: 219 2724 1135 681 191 1471
Fragments arranged by size:
2724 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4525 5426 5688 820
Size: 197 926 2582 901 262 1553
Fragments arranged by size:
2582 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3718 4212 493
Size: 3225 494 2702
Fragments arranged by size:
3225 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6421
StyI C'CwwG_G
Cuts at: 513 1065 2166 2272 2280 2388 2439 3860 513
Size: 552 1101 106 8 108 51 1421 3074
Fragments arranged by size:
3074 1421 1101 552 108 106 51 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 2237 2452 4448 4465 4618 4803
Size: 1255 525 132 215 1996 17 153 185
Cuts at: 4803 5248 325
Size: 445 1498
Fragments arranged by size:
1996 1498 1255 525 445 215 185 153
132 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 4667 6379
Size: 274 80 33 51 1443 543 2147 1712
Cuts at: 6379 96
Size: 138
Fragments arranged by size:
2147 1712 1443 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 2178 2210 2899 2931 6381 6413 2055
Size: 32 91 32 689 32 3450 32 2063
Fragments arranged by size:
3450 2063 689 91 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3431 1316
Size: 2115 4306
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2600 2188
Size: 412 6009
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6421
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 4550 4550
Size: 6421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2104 4229 276
Size: 53 83 186 936 570 2125 2468
Fragments arranged by size:
2468 2125 936 570 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI
SwaI