Restriction map for
pFN21ASDB0024
(Circular) (Six-base) MAPSORT of: pFN21ASDB0024.seq Check: 1097 from: 1 to: 8470
pFN21ASDB0024 8470 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6280 278
Size: 53 83 186 936 4744 2468
Fragments arranged by size:
4744 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5754 5813 1937
Size: 3817 59 4594
Fragments arranged by size:
4594 3817 59
Acc65I G'GTAC_C
Cuts at: 4124 5791 4124
Size: 1667 6803
AclI AA'CG_TT
Cuts at: 1563 2890 4959 6597 6970 1563
Size: 1327 2069 1638 373 3063
Fragments arranged by size:
3063 2069 1638 1327 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 3264 3312 3965 4077 4608 4908 4935
Size: 563 1069 48 653 112 531 300 27
Cuts at: 4935 5578 6533 7917 8185 1632
Size: 643 955 1384 268 1917
Fragments arranged by size:
1917 1384 1069 955 653 643 563 531
300 268 112 48 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8273
AflIII A'CryG_T
Cuts at: 1203 5334 7369 8346 1203
Size: 4131 2035 977 1327
Fragments arranged by size:
4131 2035 1327 977
AgeI A'CCGG_T
Cuts at: 7301 7301
Size: 8470
AhdI GACnn_n'nnGTC
Cuts at: 2771 3718 7199 2771
Size: 947 3481 4042
Fragments arranged by size:
4042 3481 947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3791 4883 1184
Size: 2607 1092 4771
Fragments arranged by size:
4771 2607 1092
AlwNI CAG_nnn'CTG
Cuts at: 5062 6213 7785 5062
Size: 1151 1572 5747
Fragments arranged by size:
5747 1572 1151
ApaI G_GGCC'C
Cuts at: 5038 5038
Size: 8470
ApaLI G'TGCA_C
Cuts at: 6527 7683 6527
Size: 1156 7314
ApoI r'AATT_y
Cuts at: 1493 2327 4102 5779 6005 6157 8266 1493
Size: 834 1775 1677 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 1677 834 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2049 7024 160
Size: 872 1017 4975 1606
Fragments arranged by size:
4975 1606 1017 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8470
AvaI C'yCGr_G
Cuts at: 1870 1943 2910 4448 5795 1870
Size: 73 967 1538 1347 4545
Fragments arranged by size:
4545 1538 1347 967 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4108 4141 4108
Size: 33 8437
BamHI G'GATC_C
Cuts at: 2726 3701 5800 6167 2726
Size: 975 2099 367 5029
Fragments arranged by size:
5029 2099 975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4124 4805 5791 7246 618
Size: 325 217 627 2337 681 986 1455 1842
Fragments arranged by size:
2337 1842 1455 986 681 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4366 5038 5789 1391
Size: 2975 672 751 4072
Fragments arranged by size:
4072 2975 751 672
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 3757 4509 4657 5308 928
Size: 971 248 1610 752 148 651 4090
Fragments arranged by size:
4090 1610 971 752 651 248 148
BbvCI CC'TCA_GC
Cuts at: 3676 3676
Size: 8470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6661 6695 1903
Size: 34 4724 34 3678
Fragments arranged by size:
4724 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2533 3415 5364 6362 7572 2533
Size: 882 1949 998 1210 3431
Fragments arranged by size:
3431 1949 1210 998 882
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4095 7081 136
Size: 107 122 71 631 605 2423 2986 1525
Fragments arranged by size:
2986 2423 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3599 5194 8465 3599
Size: 1595 3271 3604
Fragments arranged by size:
3604 3271 1595
BlpI GC'TnA_GC
Cuts at: 5470 5887 5470
Size: 417 8053
Bme1580I G_kGCm'C
Cuts at: 1790 5038 6531 7687 1790
Size: 3248 1493 1156 2573
Fragments arranged by size:
3248 2573 1493 1156
BmgBI CAC'GTC
Cuts at: 1318 5335 5600 1318
Size: 4017 265 4188
Fragments arranged by size:
4188 4017 265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 4173 4705
Size: 416 53 83 399 24 1083 1673 532
Cuts at: 4705 5032 6201 7159 442
Size: 327 1169 958 1753
Fragments arranged by size:
1753 1673 1169 1083 958 532 416 399
327 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3197 3971 1056
Size: 2141 774 5555
Fragments arranged by size:
5555 2141 774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 8470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2456 4983 5632 7130 1239
Size: 986 231 2527 649 1498 2579
Fragments arranged by size:
2579 2527 1498 986 649 231
Bpu10I CC'TnA_GC
Cuts at: 3676 4599 3676
Size: 923 7547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 3209 4454 4617 5432 6595 7460 7758 2528
Size: 681 1245 163 815 1163 865 298 3240
Fragments arranged by size:
3240 1245 1163 865 815 681 298 163
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 7133 882
Size: 617 669 4965 2219
Fragments arranged by size:
4965 2219 669 617
BsaAI yAC'GTr
Cuts at: 493 1204 4774 5767 6261 493
Size: 711 3570 993 494 2702
Fragments arranged by size:
3570 2702 993 711 494
BsaBI GATnn'nnATC
Cuts at: 5079 8464 5079
Size: 3385 5085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5742 6277 6659 7294
Size: 53 83 186 936 4209 535 382 635
Cuts at: 7294 275
Size: 1451
Fragments arranged by size:
4209 1451 936 635 535 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2323 5044 6903 7301 7575 7722 8133 2323
Size: 2721 1859 398 274 147 411 2660
Fragments arranged by size:
2721 2660 1859 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 4330 4360 1178
Size: 30 165 30 687 30 2210 30 5288
Fragments arranged by size:
5288 2210 687 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3413 4335 4338 4920 5619 1186
Size: 2227 922 3 582 699 4037
Fragments arranged by size:
4037 2227 922 699 582 3
BseYI C'CCAG_C
Cuts at: 2199 5031 5111 7673 8169 2199
Size: 2832 80 2562 496 2500
Fragments arranged by size:
2832 2562 2500 496 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 2616 1163
Size: 573 583 297 7017
Fragments arranged by size:
7017 583 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5920 6681 7709 1540
Size: 275 186 3919 761 1028 2301
Fragments arranged by size:
3919 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 3073 6531 6616 7687 2571
Size: 502 3458 85 1071 3354
Fragments arranged by size:
3458 3354 1071 502 85
BsmI GAATG_Cn'
Cuts at: 4764 5156 5981 6074 4764
Size: 392 825 93 7160
Fragments arranged by size:
7160 825 392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2571 3073 4366 5038 5789 6531 6616
Size: 399 781 502 1293 672 751 742 85
Cuts at: 6616 7687 1391
Size: 1071 2174
Fragments arranged by size:
2174 1293 1071 781 751 742 672 502
399 85
BspEI T'CCGG_A
Cuts at: 8133 8133
Size: 8470
BspHI T'CATG_A
Cuts at: 4245 4972 5083 6359 8089 4245
Size: 727 111 1276 1730 4626
Fragments arranged by size:
4626 1730 1276 727 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5825 844
Size: 4981 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5418 6357 1394
Size: 429 3595 939 3507
Fragments arranged by size:
3595 3507 939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5356 6965 7139 65
Size: 1183 4108 1609 174 1396
Fragments arranged by size:
4108 1609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7114 7301 1700
Size: 110 5304 187 2869
Fragments arranged by size:
5304 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8470
BssSI C'ACGA_G
Cuts at: 3815 5631 6530 7542 3815
Size: 1816 899 1012 4743
Fragments arranged by size:
4743 1816 1012 899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8470
BstBI TT'CG_AA
Cuts at: 2007 7274 2007
Size: 5267 3203
BstEII G'GTnAC_C
Cuts at: 1184 5121 1184
Size: 3937 4533
BstXI CCAn_nnnn'nTGG
Cuts at: 2090 4228 4718 2090
Size: 2138 490 5842
Fragments arranged by size:
5842 2138 490
BstYI r'GATC_y
Cuts at: 1972 2726 2953 3599 3701 3928 4836 5194 5800
Size: 754 227 646 102 227 908 358 606
Cuts at: 5800 6167 6552 6569 8021 8107 8119 8465 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 908 754 646 606 385 367
358 346 227 227 102 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2515 2515
Size: 8470
BtgI C'CryG_G
Cuts at: 513 1065 3009 513
Size: 552 1944 5974
Fragments arranged by size:
5974 1944 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4140 4856 487
Size: 683 56 2914 716 4101
Fragments arranged by size:
4101 2914 716 683 56
BtsI GCAGTG_nn'
Cuts at: 2095 4250 4865 5986 6780 6800 2095
Size: 2155 615 1121 794 20 3765
Fragments arranged by size:
3765 2155 1121 794 615 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8435
DraI TTT'AAA
Cuts at: 5211 5774 5211
Size: 563 7907
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7477 809
Size: 507 6161 1802
Fragments arranged by size:
6161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4161 5917 6806
Size: 54 1425 50 275 18 2331 1756 889
Cuts at: 6806 8435 8
Size: 1629 43
Fragments arranged by size:
2331 1756 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5917 1537
Size: 275 4105 4090
Fragments arranged by size:
4105 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3153 3288 3342 3775 4488 4793 6400 1353
Size: 1800 135 54 433 713 305 1607 3423
Fragments arranged by size:
3423 1800 1607 713 433 305 135 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7057 7431 7577 387
Size: 1064 138 5468 374 146 1280
Fragments arranged by size:
5468 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2225 2456 3264 3312 3965 4077
Size: 393 563 30 231 808 48 653 112
Cuts at: 4077 4608 4908 4935 4983 5578 5632 6533 7130
Size: 531 300 27 48 595 54 901 597
Cuts at: 7130 7917 8185 1239
Size: 787 268 1524
Fragments arranged by size:
1524 901 808 787 653 597 595 563
531 393 300 268 231 112 54 48
48 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 3284 5614 2267
Size: 1017 2330 5123
Fragments arranged by size:
5123 2330 1017
EcoO109I rG'GnC_Cy
Cuts at: 2079 5664 2079
Size: 3585 4885
EcoRI G'AATT_C
Cuts at: 5779 6157 5779
Size: 378 8092
EcoRV GAT'ATC
Cuts at: 2275 2275
Size: 8470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2867 3203 4499 4574 2867
Size: 336 1296 75 6763
Fragments arranged by size:
6763 1296 336 75
FspI TGC'GCA
Cuts at: 6976 6976
Size: 8470
HaeII r_GCGC'y
Cuts at: 1678 7617 1678
Size: 5939 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2219 2251 2264
Size: 32 135 32 518 32 97 32 13
Cuts at: 2264 2296 2757 2789 3704 3736 3920 3952 4258
Size: 32 461 32 915 32 184 32 306
Cuts at: 4258 4290 4366 4398 4878 4910 7111 7143 7185
Size: 32 76 32 480 32 2201 32 42
Cuts at: 7185 7217 1373
Size: 32 2626
Fragments arranged by size:
2626 2201 915 518 480 461 306 184
135 97 76 42 32 32 32 32
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3341 5697 5814 6060 669
Size: 988 281 1403 2356 117 246 3079
Fragments arranged by size:
3079 2356 1403 988 281 246 117
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8470
HpaI GTT'AAC
Cuts at: 5697 6060 5697
Size: 363 8107
KpnI G_GTAC'C
Cuts at: 4128 5795 4128
Size: 1667 6803
MfeI C'AATT_G
Cuts at: 3614 3989 5379 6069 3614
Size: 375 1390 690 6015
Fragments arranged by size:
6015 1390 690 375
MluI A'CGCG_T
Cuts at: 8346 8346
Size: 8470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4958 5516 7584 7768 1305
Size: 3653 558 2068 184 2007
Fragments arranged by size:
3653 2068 2007 558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4163 10
Size: 54 1425 343 2331 4317
Fragments arranged by size:
4317 2331 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 3773 3878 3954 4404 6428 6787
Size: 648 897 1710 105 76 450 2024 359
Cuts at: 6787 6946 518
Size: 159 2042
Fragments arranged by size:
2042 2024 1710 897 648 450 359 159
105 76
MspA1I CmG'CkG
Cuts at: 1606 2122 3680 5684 5886 6563 7711 7956 1606
Size: 516 1558 2004 202 677 1148 245 2120
Fragments arranged by size:
2120 2004 1558 1148 677 516 245 202
NcoI C'CATG_G
Cuts at: 513 1065 3009 513
Size: 552 1944 5974
Fragments arranged by size:
5974 1944 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8470
NheI G'CTAG_C
Cuts at: 1052 3193 3967 1052
Size: 2141 774 5555
Fragments arranged by size:
5555 2141 774
NotI GC'GGCC_GC
Cuts at: 5917 5917
Size: 8470
NspI r_CATG'y
Cuts at: 1132 1229 5828 7373 1132
Size: 97 4599 1545 2229
Fragments arranged by size:
4599 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4609 5551 1348
Size: 3261 942 4267
Fragments arranged by size:
4267 3261 942
PciI A'CATG_T
Cuts at: 7369 7369
Size: 8470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4903 1108
Size: 3795 4675
PfoI T'CCnGG_A
Cuts at: 4896 4896
Size: 8470
PmeI GTTT'AAAC
Cuts at: 5774 5774
Size: 8470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6539 6571 8052 8084 6539
Size: 32 1481 32 6925
Fragments arranged by size:
6925 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 5664 2079
Size: 3585 4885
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8470
PsiI TTA'TAA
Cuts at: 6040 6040
Size: 8470
PspOMI G'GGCC_C
Cuts at: 5034 5034
Size: 8470
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3636 3668 3636
Size: 32 8438
PstI C_TGCA'G
Cuts at: 830 2363 4597 5822 830
Size: 1533 2234 1225 3478
Fragments arranged by size:
3478 2234 1533 1225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8470
PvuII CAG'CTG
Cuts at: 2122 3680 5684 2122
Size: 1558 2004 4908
Fragments arranged by size:
4908 2004 1558
SalI G'TCGA_C
Cuts at: 1936 5812 1936
Size: 3876 4594
SanDI GG'GwC_CC
Cuts at: 2079 2079
Size: 8470
SbfI CC_TGCA'GG
Cuts at: 2363 5822 2363
Size: 3459 5011
ScaI AGT'ACT
Cuts at: 3846 6718 8430 3846
Size: 2872 1712 3886
Fragments arranged by size:
3886 2872 1712
SfcI C'TryA_G
Cuts at: 826 1045 2359 2802 4041 4593 5054 5461 5818
Size: 219 1314 443 1239 552 461 407 357
Cuts at: 5818 6953 7634 7825 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1314 1239 1135 681 552 461 443
407 357 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8470
SmaI CCC'GGG
Cuts at: 1872 5797 1872
Size: 3925 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 3188 4433 4632 5411 6574
Size: 197 926 600 645 1245 199 779 1163
Cuts at: 6574 7475 7737 820
Size: 901 262 1553
Fragments arranged by size:
1553 1245 1163 926 901 779 645 600
262 199 197
SnaBI TAC'GTA
Cuts at: 493 5767 6261 493
Size: 5274 494 2702
Fragments arranged by size:
5274 2702 494
SpeI A'CTAG_T
Cuts at: 152 2822 152
Size: 2670 5800
SphI G_CATG'C
Cuts at: 1132 5828 1132
Size: 4696 3774
SspI AAT'ATT
Cuts at: 5 52 3310 6394 5
Size: 47 3258 3084 2081
Fragments arranged by size:
3258 3084 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 4098 1517
Size: 1062 1519 5889
Fragments arranged by size:
5889 1519 1062
StyI C'CwwG_G
Cuts at: 513 1065 2580 3009 3285 3755 5909 513
Size: 552 1515 429 276 470 2154 3074
Fragments arranged by size:
3074 2154 1515 552 470 429 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2244 3761 4134 4954 5370 5519 6497
Size: 1255 664 1517 373 820 416 149 978
Cuts at: 6497 6514 6667 6852 7297 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1517 1498 1255 978 820 664 445 416
373 185 153 149 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3844 5130 5638
Size: 274 80 33 51 1443 1867 1286 508
Cuts at: 5638 6716 8428 96
Size: 1078 1712 138
Fragments arranged by size:
1867 1712 1443 1286 1078 508 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3693 3725 3803 3835 8430 8462 3693
Size: 32 78 32 4595 32 3701
Fragments arranged by size:
4595 3701 78 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8470
XbaI T'CTAG_A
Cuts at: 2158 2374 3493 3667 5806 2158
Size: 216 1119 174 2139 4822
Fragments arranged by size:
4822 2139 1119 216 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4847 4847
Size: 8470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8470
XmaI C'CCGG_G
Cuts at: 1870 5795 1870
Size: 3925 4545
XmnI GAAnn'nnTTC
Cuts at: 2370 4948 5106 6599 2370
Size: 2578 158 1493 4241
Fragments arranged by size:
4241 2578 1493 158
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6278 276
Size: 53 83 186 936 4744 2468
Fragments arranged by size:
4744 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI