Restriction map for
pFN21ASDB0022
(Circular) (Six-base) MAPSORT of: pFN21ASDB0022.seq Check: 628 from: 1 to: 6289
pFN21ASDB0022 6289 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 3632 1937
Size: 993 702 4594
Fragments arranged by size:
4594 993 702
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4352 5736 6004 1632
Size: 2720 1384 268 1917
Fragments arranged by size:
2720 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 5188 6165 1203
Size: 3985 977 1327
Fragments arranged by size:
3985 1327 977
AgeI A'CCGG_T
Cuts at: 3346 5120 3346
Size: 1774 4515
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 2951 3294 1166
Size: 1785 343 4161
Fragments arranged by size:
4161 1785 343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3299 1184
Size: 2115 4174
AlwNI CAG_nnn'CTG
Cuts at: 3530 4032 5604 3530
Size: 502 1572 4215
Fragments arranged by size:
4215 1572 502
ApaI G_GGCC'C
Cuts at: 3498 3498
Size: 6289
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2706 3271 3614 1870
Size: 73 375 388 565 343 4545
Fragments arranged by size:
4545 565 388 375 343 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3561 3594 3561
Size: 33 6256
BamHI G'GATC_C
Cuts at: 3449 3619 3986 3449
Size: 170 367 5752
Fragments arranged by size:
5752 367 170
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2458 3610 5065 618
Size: 325 217 627 671 1152 1455 1842
Fragments arranged by size:
1842 1455 1152 671 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2325 2354 2394 2403 2480 2812 3054 3498
Size: 934 29 40 9 77 332 242 444
Cuts at: 3498 3545 3608 1391
Size: 47 63 4072
Fragments arranged by size:
4072 934 444 332 242 77 63 47
40 29 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5318
BbvCI CC'TCA_GC
Cuts at: 3204 3225 3537 3204
Size: 21 312 5956
Fragments arranged by size:
5956 312 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4480 4514 1903
Size: 34 2543 34 3678
Fragments arranged by size:
3678 2543 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BfrBI ATG'CAT
Cuts at: 2869 2869
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2614 4900 136
Size: 107 122 71 631 605 942 2286 1525
Fragments arranged by size:
2286 1525 942 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2893 6284 2893
Size: 3391 2898
BlpI GC'TnA_GC
Cuts at: 2126 3544 3706 2126
Size: 1418 162 4709
Fragments arranged by size:
4709 1418 162
Bme1580I G_kGCm'C
Cuts at: 1790 2463 3498 4350 5506 1790
Size: 673 1035 852 1156 2573
Fragments arranged by size:
2573 1156 1035 852 673
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2920 2945 3512
Size: 416 53 83 399 24 1503 25 567
Cuts at: 3512 4020 4978 442
Size: 508 958 1753
Fragments arranged by size:
1753 1503 958 567 508 416 399 83
53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 3372 4949 1239
Size: 1142 991 1577 2579
Fragments arranged by size:
2579 1577 1142 991
Bpu10I CC'TnA_GC
Cuts at: 3204 3225 3414 3537 3204
Size: 21 189 123 5956
Fragments arranged by size:
5956 189 123 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 4414 5279 5577 2207
Size: 2207 865 298 2919
Fragments arranged by size:
2919 2207 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 2926 3434 3459 4952 882
Size: 617 774 653 508 25 1493 2219
Fragments arranged by size:
2219 1493 774 653 617 508 25
BsaAI yAC'GTr
Cuts at: 493 1204 3292 3586 4080 493
Size: 711 2088 294 494 2702
Fragments arranged by size:
2702 2088 711 494 294
BsaBI GATnn'nnATC
Cuts at: 2042 6283 2042
Size: 4241 2048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4096 4478 5113 275
Size: 53 83 186 936 2563 382 635 1451
Fragments arranged by size:
2563 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3346 4722 5120 5394 5541 5952 3346
Size: 1376 398 274 147 411 3683
Fragments arranged by size:
3683 1376 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2354 2384 1178
Size: 30 165 30 951 30 5083
Fragments arranged by size:
5083 951 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2714 3046 1186
Size: 1216 312 332 4429
Fragments arranged by size:
4429 1216 332 312
BseYI C'CCAG_C
Cuts at: 2498 2565 2610 2638 2675 2799 2813 2907 3132
Size: 67 45 28 37 124 14 94 225
Cuts at: 3132 3400 3439 3517 5492 5988 2498
Size: 268 39 78 1975 496 2799
Fragments arranged by size:
2799 1975 496 268 225 124 94 78
67 45 39 37 28 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3066 1163
Size: 573 1330 4386
Fragments arranged by size:
4386 1330 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3739 4500 5528 1540
Size: 275 186 1738 761 1028 2301
Fragments arranged by size:
2301 1738 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 2480 2707 3054 4350 4435 5506 2394
Size: 86 227 347 1296 85 1071 3177
Fragments arranged by size:
3177 1296 1071 347 227 86 85
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 3452 3452
Size: 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2354 2394 2403 2463 2480 2707
Size: 399 535 29 40 9 60 17 227
Cuts at: 2707 2812 3054 3498 3545 3608 4350 4435 5506
Size: 105 242 444 47 63 742 85 1071
Cuts at: 5506 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 535 444 399 242 227
105 85 63 60 47 40 29 17
9
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3644 844
Size: 1994 806 3489
Fragments arranged by size:
3489 1994 806
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 4176 1394
Size: 429 242 2111 3507
Fragments arranged by size:
3507 2111 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 3106 4784 4958 65
Size: 1183 1858 1678 174 1396
Fragments arranged by size:
1858 1678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3346 4933 5120 1700
Size: 110 1536 1587 187 2869
Fragments arranged by size:
2869 1587 1536 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 2249 4349 5361 2249
Size: 2100 1012 3177
Fragments arranged by size:
3177 2100 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6289
BstBI TT'CG_AA
Cuts at: 2007 5093 2007
Size: 3086 3203
BstEII G'GTnAC_C
Cuts at: 1184 2512 2645 1184
Size: 1328 133 4828
Fragments arranged by size:
4828 1328 133
BstXI CCAn_nnnn'nTGG
Cuts at: 2915 2953 2915
Size: 38 6251
BstYI r'GATC_y
Cuts at: 1972 2043 2893 3449 3619 3986 4371 4388 5840
Size: 71 850 556 170 367 385 17 1452
Cuts at: 5840 5926 5938 6284 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 850 556 385 367 346 170
86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 2285 3531 2285
Size: 1246 5043
BtgI C'CryG_G
Cuts at: 513 1065 2022 2549 513
Size: 552 957 527 4253
Fragments arranged by size:
4253 957 552 527
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3438 487
Size: 683 56 2212 3338
Fragments arranged by size:
3338 2212 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 3012 3105 3805 4599 4619 2480
Size: 532 93 700 794 20 4150
Fragments arranged by size:
4150 794 700 532 93 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2834 5296 809
Size: 507 1518 2462 1802
Fragments arranged by size:
2462 1802 1518 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2615 2733 3736
Size: 54 1425 50 275 18 785 118 1003
Cuts at: 3736 4625 6254 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1003 889 785 275 118 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3736 1537
Size: 275 1924 4090
Fragments arranged by size:
4090 1924 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2408 3007 3548 4219 1353
Size: 1055 599 541 671 3423
Fragments arranged by size:
3423 1055 671 599 541
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2766 4876 5250 5396 387
Size: 1064 138 1177 2110 374 146 1280
Fragments arranged by size:
2110 1280 1177 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2478 3052 2392
Size: 86 574 5629
Fragments arranged by size:
5629 574 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 3372 4352 4949 5736 6004 1239
Size: 393 749 991 980 597 787 268 1524
Fragments arranged by size:
1524 991 980 787 749 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2753 2360
Size: 393 5896
EcoO109I rG'GnC_Cy
Cuts at: 2989 2989
Size: 6289
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 6289
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 2413 2914 5436 1678
Size: 735 501 2522 2531
Fragments arranged by size:
2531 2522 735 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373
Size: 32 135 32 3358 32 42 32 2626
Fragments arranged by size:
3358 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 3039 3633 3879 669
Size: 988 281 483 618 594 246 3079
Fragments arranged by size:
3079 988 618 594 483 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 5403 5587 1305
Size: 894 3204 184 2007
Fragments arranged by size:
3204 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 10
Size: 54 1425 343 903 3564
Fragments arranged by size:
3564 1425 903 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2951 3294 4247 4606 4765 518
Size: 648 1038 747 343 953 359 159 2042
Fragments arranged by size:
2042 1038 953 747 648 359 343 159
MspA1I CmG'CkG
Cuts at: 1606 2502 2551 2614 3392 3404 3548 3705 4382
Size: 896 49 63 778 12 144 157 677
Cuts at: 4382 5530 5775 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 896 778 677 245 157 144
63 49 12
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4780
Fragments arranged by size:
4780 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NsiI A_TGCA'T
Cuts at: 2871 2871
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 3647 5192 1132
Size: 97 2418 1545 2229
Fragments arranged by size:
2418 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6289
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6289
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PmlI CAC'GTG
Cuts at: 3292 3292
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6289
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 3494 3494
Size: 6289
PstI C_TGCA'G
Cuts at: 830 2487 3641 830
Size: 1657 1154 3478
Fragments arranged by size:
3478 1657 1154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 3392 3404 3548 3392
Size: 12 144 6133
Fragments arranged by size:
6133 144 12
SacI G_AGCT'C
Cuts at: 2394 2480 3054 2394
Size: 86 574 5629
Fragments arranged by size:
5629 574 86
SacII CC_GC'GG
Cuts at: 2552 2552
Size: 6289
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SapI GCTCTTCn'nnn_
Cuts at: 2408 2408
Size: 6289
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SexAI A'CCwGG_T
Cuts at: 3240 3240
Size: 6289
SfcI C'TryA_G
Cuts at: 826 1045 2483 2579 3637 4772 5453 5644 826
Size: 219 1438 96 1058 1135 681 191 1471
Fragments arranged by size:
1471 1438 1135 1058 681 219 191 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3273 3616 1872
Size: 1401 343 4545
Fragments arranged by size:
4545 1401 343
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 4393 5294 5556 820
Size: 197 926 279 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 279 262 197
SnaBI TAC'GTA
Cuts at: 493 3586 4080 493
Size: 3093 494 2702
Fragments arranged by size:
3093 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3647 1132
Size: 2515 3774
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6289
StyI C'CwwG_G
Cuts at: 513 1065 2022 2914 3728 513
Size: 552 957 892 814 3074
Fragments arranged by size:
3074 957 892 814 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2813 3284 3332 4316 4333 4486 4671
Size: 1255 1233 471 48 984 17 153 185
Cuts at: 4671 5116 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1233 984 471 445 185 153
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2235 4535
Size: 274 80 33 51 1443 249 9 2300
Cuts at: 4535 6247 96
Size: 1712 138
Fragments arranged by size:
2300 1712 1443 274 249 138 80 51
33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6249 6281 6249
Size: 32 6257
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2506 2203
Size: 303 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 3271 3614 1870
Size: 1401 343 4545
Fragments arranged by size:
4545 1401 343
XmnI GAAnn'nnTTC
Cuts at: 2297 4418 2297
Size: 2121 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PpuMI PspXI PsrI RsrII SanDI
SfiI SfoI SrfI SwaI