Restriction map for
pFN21ASDB0021
(Circular) (Six-base) MAPSORT of: pFN21ASDB0021.seq Check: 2384 from: 1 to: 5710
pFN21ASDB0021 5710 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 3053 1937
Size: 223 893 4594
Fragments arranged by size:
4594 893 223
Acc65I G'GTAC_C
Cuts at: 2492 3031 2492
Size: 539 5171
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 5157 5425 1632
Size: 2141 1384 268 1917
Fragments arranged by size:
2141 1917 1384 268
AfeI AGC'GCT
Cuts at: 2333 2678 2333
Size: 345 5365
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2126 1184
Size: 942 4768
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2237 2519 2854 2876 2933 3035 1870
Size: 73 294 282 335 22 57 102 4545
Fragments arranged by size:
4545 335 294 282 102 73 57 22
AvrII C'CTAG_G
Cuts at: 2560 2560
Size: 5710
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2215 2371 2492 2719 3031
Size: 325 217 627 428 156 121 227 312
Cuts at: 3031 4486 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 428 325 312 227 217
156 121
BanII G_rGCy'C
Cuts at: 1391 2646 2934 3029 1391
Size: 1255 288 95 4072
Fragments arranged by size:
4072 1255 288 95
BbeI G_GCGC'C
Cuts at: 2219 2723 2219
Size: 504 5206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2506 928
Size: 971 607 4132
Fragments arranged by size:
4132 971 607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2974 3008 3901 3935 1903
Size: 34 1037 34 893 34 3678
Fragments arranged by size:
3678 1037 893 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2550 2562 2709
Size: 107 122 71 631 605 878 12 147
Cuts at: 2709 2880 2996 4321 136
Size: 171 116 1325 1525
Fragments arranged by size:
1525 1325 878 631 605 171 147 122
116 107 71 12
BglII A'GATC_T
Cuts at: 2073 2940 5705 2073
Size: 867 2765 2078
Fragments arranged by size:
2765 2078 867
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2669 3771 4927 1790
Size: 390 489 1102 1156 2573
Fragments arranged by size:
2573 1156 1102 489 390
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2412 2464 3001 4370 1239
Size: 818 355 52 537 1369 2579
Fragments arranged by size:
2579 1369 818 537 355 52
Bpu10I CC'TnA_GC
Cuts at: 2165 2165
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 3835 4700 4998 2723
Size: 1112 865 298 3435
Fragments arranged by size:
3435 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2134 2306 2801 4373 882
Size: 617 635 172 495 1572 2219
Fragments arranged by size:
2219 1572 635 617 495 172
BsaAI yAC'GTr
Cuts at: 493 1204 3007 3501 493
Size: 711 1803 494 2702
Fragments arranged by size:
2702 1803 711 494
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2216 2511 2720 3517
Size: 53 83 186 936 683 295 209 797
Cuts at: 3517 3899 4534 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 797 683 635 382 295 209
186 83 53
BsaWI w'CCGG_w
Cuts at: 2917 4143 4541 4815 4962 5373 2917
Size: 1226 398 274 147 411 3254
Fragments arranged by size:
3254 1226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2326 2793 2813 2912 2921 1186
Size: 1140 467 20 99 9 3975
Fragments arranged by size:
3975 1140 467 99 20 9
BseYI C'CCAG_C
Cuts at: 2204 2268 2505 2704 4913 5409 2204
Size: 64 237 199 2209 496 2505
Fragments arranged by size:
2505 2209 496 237 199 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 2592 3160 3921 4949 1540
Size: 275 186 252 339 568 761 1028 2301
Fragments arranged by size:
2301 1028 761 568 339 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2987 3771 3856 4927 2987
Size: 784 85 1071 3770
Fragments arranged by size:
3770 1071 784 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
BsmBI CGTCTCn'nnnn_
Cuts at: 2347 2347
Size: 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2646 2669 2934 2987 3029 3771
Size: 399 390 466 23 265 53 42 742
Cuts at: 3771 3856 4927 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 399 390 265 85
53 42 23
BspEI T'CCGG_A
Cuts at: 2917 5373 2917
Size: 2456 3254
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 844
Size: 2221 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2294 2737 2913 3597 1394
Size: 429 471 443 176 684 3507
Fragments arranged by size:
3507 684 471 443 429 176
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 2245 2264 2374 2587 2956 4354
Size: 110 385 50 19 110 213 369 1398
Cuts at: 4354 4541 1700
Size: 187 2869
Fragments arranged by size:
2869 1398 385 369 213 187 110 110
50 19
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2537 2619 1925
Size: 612 82 5016
Fragments arranged by size:
5016 612 82
BssSI C'ACGA_G
Cuts at: 2295 2529 3770 4782 2295
Size: 234 1241 1012 3223
Fragments arranged by size:
3223 1241 1012 234
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 2007 4514 2007
Size: 2507 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2073 2940 3040 3407 3792 3809 5261 5347
Size: 101 867 100 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 867 385 367 346 101 100
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2354 2382 2658 513
Size: 552 957 332 28 276 3565
Fragments arranged by size:
3565 957 552 332 276 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 487
Size: 683 56 804 4167
Fragments arranged by size:
4167 804 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2250 2306
Size: 54 1425 50 275 18 363 57 56
Cuts at: 2306 2379 2475 2589 3157 4046 5675 8
Size: 73 96 114 568 889 1629 43
Fragments arranged by size:
1629 1425 889 568 363 275 114 96
73 57 56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 2589 3157 1537
Size: 275 438 339 568 4090
Fragments arranged by size:
4090 568 438 339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 3640 1353
Size: 1536 751 3423
Fragments arranged by size:
3423 1536 751
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2430 2438 4297 4671 4817 387
Size: 1064 138 841 8 1859 374 146 1280
Fragments arranged by size:
1859 1280 1064 841 374 146 138 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2412 2464 3001 3773 4370 5157
Size: 393 425 355 52 537 772 597 787
Cuts at: 5157 5425 1239
Size: 268 1524
Fragments arranged by size:
1524 787 772 597 537 425 393 355
268 52
EcoO109I rG'GnC_Cy
Cuts at: 2208 2572 2208
Size: 364 5346
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 2144 4216 2144
Size: 2072 3638
HaeII r_GCGC'y
Cuts at: 1678 2151 2219 2335 2680 2723 4857 1678
Size: 473 68 116 345 43 2134 2531
Fragments arranged by size:
2531 2134 473 345 116 68 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373
Size: 32 135 32 2779 32 42 32 2626
Fragments arranged by size:
2779 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2215 2719 2215
Size: 504 5206
KpnI G_GTAC'C
Cuts at: 2496 3035 2496
Size: 539 5171
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 10
Size: 54 1425 343 549 3339
Fragments arranged by size:
3339 1425 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3668 4027 4186 518
Size: 648 2502 359 159 2042
Fragments arranged by size:
2502 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2356 2633 2660 2708 3126 3803 4951
Size: 598 152 277 27 48 418 677 1148
Cuts at: 4951 5196 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 598 418 277 245 152
48 27
NaeI GCC'GGC
Cuts at: 2247 2266 2247
Size: 19 5691
NarI GG'CG_CC
Cuts at: 2216 2720 2216
Size: 504 5206
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4201
Fragments arranged by size:
4201 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NgoMIV G'CCGG_C
Cuts at: 2245 2264 2245
Size: 19 5691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2572 2208
Size: 364 5346
PshAI GACnn'nnGTC
Cuts at: 1613 2393 1613
Size: 780 4930
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
RsrII CG'GwC_CG
Cuts at: 2915 2915
Size: 5710
SacII CC_GC'GG
Cuts at: 2357 2661 2357
Size: 304 5406
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2208 2208
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 3058 4193 4874 5065 826
Size: 219 2013 1135 681 191 1471
Fragments arranged by size:
2013 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2217 2721 2217
Size: 504 5206
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 2239 2878 2935 3037 1872
Size: 367 639 57 102 4545
Fragments arranged by size:
4545 639 367 102 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 3814 4715 4977 820
Size: 197 926 795 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3007 3501 493
Size: 2514 494 2702
Fragments arranged by size:
2702 2514 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SrfI GCCC'GGGC
Cuts at: 2935 2935
Size: 5710
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2022 2560 3149 513
Size: 552 957 538 589 3074
Fragments arranged by size:
3074 957 589 552 538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 3737 3754 3907 4092 4537 325
Size: 1255 750 1407 17 153 185 445 1498
Fragments arranged by size:
1498 1407 1255 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2265 2265
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 2237 2876 2933 3035 1870
Size: 367 639 57 102 4545
Fragments arranged by size:
4545 639 367 102 57
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII SacI SapI SexAI SfiI SwaI