Restriction map for
pFN21ASDB0018
(Circular) (Six-base) MAPSORT of: pFN21ASDB0018.seq Check: 9670 from: 1 to: 7369
pFN21ASDB0018 7369 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2182 2281 2182
Size: 99 7270
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5179 278
Size: 53 83 186 936 3643 2468
Fragments arranged by size:
3643 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3927 4662 4712 1937
Size: 1990 735 50 4594
Fragments arranged by size:
4594 1990 735 50
Acc65I G'GTAC_C
Cuts at: 2339 4690 2339
Size: 2351 5018
AclI AA'CG_TT
Cuts at: 1563 2232 5496 5869 1563
Size: 669 3264 373 3063
Fragments arranged by size:
3264 3063 669 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 2991 4364 4506 5432 6816 7084 1632
Size: 1265 94 1373 142 926 1384 268 1917
Fragments arranged by size:
1917 1384 1373 1265 926 268 142 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7172
AflIII A'CryG_T
Cuts at: 1203 2782 6268 7245 1203
Size: 1579 3486 977 1327
Fragments arranged by size:
3486 1579 1327 977
AgeI A'CCGG_T
Cuts at: 6200 6200
Size: 7369
AhdI GACnn_n'nnGTC
Cuts at: 6098 6098
Size: 7369
AleI CACnn'nnGTG
Cuts at: 1166 2525 1166
Size: 1359 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7369
AlwNI CAG_nnn'CTG
Cuts at: 2113 2197 3491 3712 5112 6684 2113
Size: 84 1294 221 1400 1572 2798
Fragments arranged by size:
2798 1572 1400 1294 221 84
ApaI G_GGCC'C
Cuts at: 4131 4131
Size: 7369
ApaLI G'TGCA_C
Cuts at: 5426 6582 5426
Size: 1156 6213
ApoI r'AATT_y
Cuts at: 1493 4678 4904 5056 7165 1493
Size: 3185 226 152 2109 1697
Fragments arranged by size:
3185 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5923 160
Size: 872 4891 1606
Fragments arranged by size:
4891 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 4506 4694 1870
Size: 73 358 2205 188 4545
Fragments arranged by size:
4545 2205 358 188 73
AvrII C'CTAG_G
Cuts at: 2062 2062
Size: 7369
BamHI G'GATC_C
Cuts at: 2757 4699 5066 2757
Size: 1942 367 5060
Fragments arranged by size:
5060 1942 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2269 2339 2515 2613 3094
Size: 325 217 627 482 70 176 98 481
Cuts at: 3094 3108 4160 4690 6145 618
Size: 14 1052 530 1455 1842
Fragments arranged by size:
1842 1455 1052 627 530 482 481 325
217 176 98 70 14
BanII G_rGCy'C
Cuts at: 1391 2972 4131 4688 1391
Size: 1581 1159 557 4072
Fragments arranged by size:
4072 1581 1159 557
BbeI G_GCGC'C
Cuts at: 2519 2617 2519
Size: 98 7271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 2999 4420 928
Size: 971 237 863 1421 3877
Fragments arranged by size:
3877 1421 971 863 237
BbvCI CC'TCA_GC
Cuts at: 2265 3020 2265
Size: 755 6614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5560 5594 1903
Size: 34 3623 34 3678
Fragments arranged by size:
3678 3623 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2093 5261 6471 2093
Size: 3168 1210 2991
Fragments arranged by size:
3168 2991 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7369
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 2611 3353
Size: 107 122 71 631 605 589 350 742
Cuts at: 3353 4043 5980 136
Size: 690 1937 1525
Fragments arranged by size:
1937 1525 742 690 631 605 589 350
122 107 71
BglII A'GATC_T
Cuts at: 7364 7364
Size: 7369
BlpI GC'TnA_GC
Cuts at: 4786 4786
Size: 7369
Bme1580I G_kGCm'C
Cuts at: 1790 2681 3896 4131 4163 5430 6586 1790
Size: 891 1215 235 32 1267 1156 2573
Fragments arranged by size:
2573 1267 1215 1156 891 235 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2675 2816 3169
Size: 416 53 83 399 24 1258 141 353
Cuts at: 3169 5100 6058 442
Size: 1931 958 1753
Fragments arranged by size:
1931 1753 1258 958 416 399 353 141
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2498 2555 3067 4581 6029 1239
Size: 896 363 57 512 1514 1448 2579
Fragments arranged by size:
2579 1514 1448 896 512 363 57
Bpu10I CC'TnA_GC
Cuts at: 2265 3020 3957 3999 4065 2265
Size: 755 937 42 66 5569
Fragments arranged by size:
5569 937 755 66 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5494 6359 6657 5494
Size: 865 298 6206
Fragments arranged by size:
6206 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2317 3126 3195 3615 4345 4368 6032
Size: 617 818 809 69 420 730 23 1664
Cuts at: 6032 882
Size: 2219
Fragments arranged by size:
2219 1664 818 809 730 617 420 69
23
BsaAI yAC'GTr
Cuts at: 493 1204 2676 2783 4666 5160 493
Size: 711 1472 107 1883 494 2702
Fragments arranged by size:
2702 1883 1472 711 494 107
BsaBI GATnn'nnATC
Cuts at: 2399 3533 7363 2399
Size: 1134 3830 2405
Fragments arranged by size:
3830 2405 1134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2516 2614 3315 5176
Size: 53 83 186 936 983 98 701 1861
Cuts at: 5176 5558 6193 275
Size: 382 635 1451
Fragments arranged by size:
1861 1451 983 936 701 635 382 186
98 83 53
BsaWI w'CCGG_w
Cuts at: 2754 2763 4308 5802 6200 6474 6621 7032 2754
Size: 9 1545 1494 398 274 147 411 3091
Fragments arranged by size:
3091 1545 1494 411 398 274 147 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3324 3354 3473 3503 3764
Size: 30 165 30 1921 30 119 30 261
Cuts at: 3764 3794 3868 3898 4509 4539 1178
Size: 30 74 30 611 30 4008
Fragments arranged by size:
4008 1921 611 261 165 119 74 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 2555 2564 2567 3248 3854 4547 4550
Size: 1366 3 9 3 681 606 693 3
Cuts at: 4550 4553 4568 4661 4664 1186
Size: 3 15 93 3 3891
Fragments arranged by size:
3891 1366 693 681 606 93 15 9
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2028 2331 2482 2796 2937 3152 3167 3303 3412
Size: 303 151 314 141 215 15 136 109
Cuts at: 3412 3659 3944 4480 6572 7068 2028
Size: 247 285 536 2092 496 2329
Fragments arranged by size:
2329 2092 536 496 314 303 285 247
215 151 141 136 109 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2098 2549 2656 2672 2705 2827 2918
Size: 573 362 451 107 16 33 122 91
Cuts at: 2918 1163
Size: 5614
Fragments arranged by size:
5614 573 451 362 122 107 91 33
16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4819 5580 6608 1540
Size: 275 186 2818 761 1028 2301
Fragments arranged by size:
2818 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5430 5515 6586 5430
Size: 85 1071 6213
Fragments arranged by size:
6213 1071 85
BsmI GAATG_Cn'
Cuts at: 4880 4973 4880
Size: 93 7276
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 2417
Size: 7369
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2681 2972 3896 4131 4163 4688 5430
Size: 399 891 291 924 235 32 525 742
Cuts at: 5430 5515 6586 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 924 891 742 525 399 291
235 85 32
BspEI T'CCGG_A
Cuts at: 2754 2763 4308 7032 2754
Size: 9 1545 2724 3091
Fragments arranged by size:
3091 2724 1545 9
BspHI T'CATG_A
Cuts at: 3286 5258 6988 3286
Size: 1972 1730 3667
Fragments arranged by size:
3667 1972 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2182 2281 4724 844
Size: 1338 99 2443 3489
Fragments arranged by size:
3489 2443 1338 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2961 4571 5256 1394
Size: 429 1138 1610 685 3507
Fragments arranged by size:
3507 1610 1138 685 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2326 5864 6038 65
Size: 1183 1078 3538 174 1396
Fragments arranged by size:
3538 1396 1183 1078 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6013 6200 1700
Size: 110 4203 187 2869
Fragments arranged by size:
4203 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7369
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7369
BssSI C'ACGA_G
Cuts at: 2525 5429 6441 2525
Size: 2904 1012 3453
Fragments arranged by size:
3453 2904 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7369
BstBI TT'CG_AA
Cuts at: 2007 6173 2007
Size: 4166 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7369
BstXI CCAn_nnnn'nTGG
Cuts at: 3091 4417 3091
Size: 1326 6043
BstYI r'GATC_y
Cuts at: 1972 2757 3528 4699 5066 5451 5468 6920 7006
Size: 785 771 1171 367 385 17 1452 86
Cuts at: 7006 7018 7364 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1171 785 771 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3104 3431 4185 4644 3104
Size: 327 754 459 5829
Fragments arranged by size:
5829 754 459 327
BtgI C'CryG_G
Cuts at: 513 1065 2694 513
Size: 552 1629 5188
Fragments arranged by size:
5188 1629 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6630
Fragments arranged by size:
6630 683 56
BtsI GCAGTG_nn'
Cuts at: 2668 3925 3956 4885 5679 5699 2668
Size: 1257 31 929 794 20 4338
Fragments arranged by size:
4338 1257 929 794 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7369
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7334
DraI TTT'AAA
Cuts at: 4673 4673
Size: 7369
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3722 6376 809
Size: 507 2406 2654 1802
Fragments arranged by size:
2654 2406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4816 5705 7334
Size: 54 1425 50 275 18 2986 889 1629
Cuts at: 7334 8
Size: 43
Fragments arranged by size:
2986 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4816 1537
Size: 275 3004 4090
Fragments arranged by size:
4090 3004 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 3831 5299 1353
Size: 1657 821 1468 3423
Fragments arranged by size:
3423 1657 1468 821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5956 6330 6476 387
Size: 1064 138 4367 374 146 1280
Fragments arranged by size:
4367 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2498 2555 2897 2991 3067 4364
Size: 393 503 363 57 342 94 76 1297
Cuts at: 4364 4506 4581 5432 6029 6816 7084 1239
Size: 142 75 851 597 787 268 1524
Fragments arranged by size:
1524 1297 851 787 597 503 393 363
342 268 142 94 76 75 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2037 2264 3513 3836 4188 2037
Size: 227 1249 323 352 5218
Fragments arranged by size:
5218 1249 352 323 227
EcoO109I rG'GnC_Cy
Cuts at: 2058 3024 3078 3751 3968 4005 4177 4234 4324
Size: 966 54 673 217 37 172 57 90
Cuts at: 4324 4603 2058
Size: 279 4824
Fragments arranged by size:
4824 966 673 279 217 172 90 57
54 37
EcoRI G'AATT_C
Cuts at: 4678 5056 4678
Size: 378 6991
EcoRV GAT'ATC
Cuts at: 3372 3372
Size: 7369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4652 4652
Size: 7369
FspI TGC'GCA
Cuts at: 2444 5875 2444
Size: 3431 3938
HaeII r_GCGC'y
Cuts at: 1678 2503 2519 2606 2617 6516 1678
Size: 825 16 87 11 3899 2531
Fragments arranged by size:
3899 2531 825 87 16 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2912 2944 3473
Size: 32 135 32 776 32 532 32 529
Cuts at: 3473 3505 3612 3644 3868 3900 4247 4279 4637
Size: 32 107 32 224 32 347 32 358
Cuts at: 4637 4669 6010 6042 6084 6116 1373
Size: 32 1341 32 42 32 2626
Fragments arranged by size:
2626 1341 776 532 529 358 347 224
135 107 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4713 4959 669
Size: 988 281 2775 246 3079
Fragments arranged by size:
3079 2775 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7369
HpaI GTT'AAC
Cuts at: 4959 4959
Size: 7369
KasI G'GCGC_C
Cuts at: 2515 2613 2515
Size: 98 7271
KpnI G_GTAC'C
Cuts at: 2343 4694 2343
Size: 2351 5018
MfeI C'AATT_G
Cuts at: 4968 4968
Size: 7369
MluI A'CGCG_T
Cuts at: 7245 7245
Size: 7369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 3527 6483 6667 1305
Size: 1455 767 2956 184 2007
Fragments arranged by size:
2956 2007 1455 767 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5547
Fragments arranged by size:
5547 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 4166 5327 5686 5845 518
Size: 648 1359 1641 1161 359 159 2042
Fragments arranged by size:
2042 1641 1359 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2533 2841 3152 3303 3712 4073 4785 5462
Size: 927 308 311 151 409 361 712 677
Cuts at: 5462 6610 6855 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 927 712 677 409 361 311
308 245 151
NarI GG'CG_CC
Cuts at: 2516 2614 2516
Size: 98 7271
NcoI C'CATG_G
Cuts at: 513 1065 2694 513
Size: 552 1629 5188
Fragments arranged by size:
5188 1629 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7369
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7369
NotI GC'GGCC_GC
Cuts at: 4816 4816
Size: 7369
NruI TCG'CGA
Cuts at: 2151 2151
Size: 7369
NspI r_CATG'y
Cuts at: 1132 1229 4284 4727 6272 1132
Size: 97 3055 443 1545 2229
Fragments arranged by size:
3055 2229 1545 443 97
PasI CC'CwG_GG
Cuts at: 1348 3258 1348
Size: 1910 5459
PciI A'CATG_T
Cuts at: 6268 6268
Size: 7369
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 2818 3279 3712 3727 3994 1108
Size: 1668 42 461 433 15 267 4483
Fragments arranged by size:
4483 1668 461 433 267 42 15
PfoI T'CCnGG_A
Cuts at: 3444 3444
Size: 7369
PmeI GTTT'AAAC
Cuts at: 4673 4673
Size: 7369
PmlI CAC'GTG
Cuts at: 2676 2676
Size: 7369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5438 5470 6951 6983 5438
Size: 32 1481 32 5824
Fragments arranged by size:
5824 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 3751 3968 4005 4177 4324 2058
Size: 1693 217 37 172 147 5103
Fragments arranged by size:
5103 1693 217 172 147 37
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7369
PsiI TTA'TAA
Cuts at: 4939 4939
Size: 7369
PspOMI G'GGCC_C
Cuts at: 4127 4127
Size: 7369
PstI C_TGCA'G
Cuts at: 830 2193 4721 830
Size: 1363 2528 3478
Fragments arranged by size:
3478 2528 1363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7369
PvuII CAG'CTG
Cuts at: 2533 3152 3303 3712 4073 2533
Size: 619 151 409 361 5829
Fragments arranged by size:
5829 619 409 361 151
SalI G'TCGA_C
Cuts at: 1936 4711 1936
Size: 2775 4594
SanDI GG'GwC_CC
Cuts at: 4005 4324 4005
Size: 319 7050
SbfI CC_TGCA'GG
Cuts at: 2193 4721 2193
Size: 2528 4841
ScaI AGT'ACT
Cuts at: 5617 7329 5617
Size: 1712 5657
SfcI C'TryA_G
Cuts at: 826 1045 2189 3761 3826 4717 5852 6533 6724
Size: 219 1144 1572 65 891 1135 681 191
Cuts at: 6724 826
Size: 1471
Fragments arranged by size:
1572 1471 1144 1135 891 681 219 191
65
SfoI GGC'GCC
Cuts at: 2517 2615 2517
Size: 98 7271
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7369
SmaI CCC'GGG
Cuts at: 1872 2303 4508 4696 1872
Size: 431 2205 188 4545
Fragments arranged by size:
4545 2205 431 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5473 6374 6636 820
Size: 197 926 3530 901 262 1553
Fragments arranged by size:
3530 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4666 5160 493
Size: 4173 494 2702
Fragments arranged by size:
4173 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7369
SphI G_CATG'C
Cuts at: 1132 4727 1132
Size: 3595 3774
SspI AAT'ATT
Cuts at: 5 52 5293 5
Size: 47 5241 2081
Fragments arranged by size:
5241 2081 47
StuI AGG'CCT
Cuts at: 1517 3519 4384 1517
Size: 2002 865 4502
Fragments arranged by size:
4502 2002 865
StyI C'CwwG_G
Cuts at: 513 1065 2062 2694 3456 3514 3795 4155 4251
Size: 552 997 632 762 58 281 360 96
Cuts at: 4251 4275 4455 4808 513
Size: 24 180 353 3074
Fragments arranged by size:
3074 997 762 632 552 360 353 281
180 96 58 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 3027 3181 4178 5396 5413 5566
Size: 1255 1137 310 154 997 1218 17 153
Cuts at: 5566 5751 6196 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1218 1137 997 445 310 185
154 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5615 7327 96
Size: 274 80 33 51 1443 3638 1712 138
Fragments arranged by size:
3638 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2707 2739 3324 3356 7329 7361 2707
Size: 32 585 32 3973 32 2715
Fragments arranged by size:
3973 2715 585 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2360 1316
Size: 1044 6325
XbaI T'CTAG_A
Cuts at: 4705 4705
Size: 7369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3991 3991
Size: 7369
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7369
XmaI C'CCGG_G
Cuts at: 1870 2301 4506 4694 1870
Size: 431 2205 188 4545
Fragments arranged by size:
4545 2205 431 188
XmnI GAAnn'nnTTC
Cuts at: 5498 5498
Size: 7369
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5177 276
Size: 53 83 186 936 3643 2468
Fragments arranged by size:
3643 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsiWI BstZ17I
DraIII EcoICRI FseI FspAI NaeI NgoMIV NsiI PacI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI