Restriction map for
pFN21ASDB0017
(Circular) (Six-base) MAPSORT of: pFN21ASDB0017.seq Check: 2235 from: 1 to: 9949
pFN21ASDB0017 9949 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 5236 2671
Size: 2565 7384
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4871 7759 278
Size: 53 83 186 936 3335 2888 2468
Fragments arranged by size:
3335 2888 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4389 6768 7292 1937
Size: 2452 2379 524 4594
Fragments arranged by size:
4594 2452 2379 524
Acc65I G'GTAC_C
Cuts at: 7270 7270
Size: 9949
AclI AA'CG_TT
Cuts at: 1563 8076 8449 1563
Size: 6513 373 3063
Fragments arranged by size:
6513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3113 3364 3719 4160 4804 6593 8012
Size: 725 756 251 355 441 644 1789 1419
Cuts at: 8012 9396 9664 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1789 1419 1384 756 725 644 441
355 268 251
AfeI AGC'GCT
Cuts at: 5609 5790 5609
Size: 181 9768
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9752
AflIII A'CryG_T
Cuts at: 1203 2847 3773 6615 8848 9825 1203
Size: 1644 926 2842 2233 977 1327
Fragments arranged by size:
2842 2233 1644 1327 977 926
AgeI A'CCGG_T
Cuts at: 8780 8780
Size: 9949
AhdI GACnn_n'nnGTC
Cuts at: 3427 6757 8678 3427
Size: 3330 1921 4698
Fragments arranged by size:
4698 3330 1921
AleI CACnn'nnGTG
Cuts at: 1166 3776 4419 6914 1166
Size: 2610 643 2495 4201
Fragments arranged by size:
4201 2610 2495 643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3431 3592 3872 1184
Size: 2247 161 280 7261
Fragments arranged by size:
7261 2247 280 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 6620 6652 3287
Size: 32 3301 32 6584
Fragments arranged by size:
6584 3301 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2686 3355 3364 3556 5338 5581 5965 6001 7692
Size: 669 9 192 1782 243 384 36 1691
Cuts at: 7692 9264 2686
Size: 1572 3371
Fragments arranged by size:
3371 1782 1691 1572 669 384 243 192
36 9
ApaI G_GGCC'C
Cuts at: 4828 5263 4828
Size: 435 9514
ApaLI G'TGCA_C
Cuts at: 7124 8006 9162 7124
Size: 882 1156 7911
Fragments arranged by size:
7911 1156 882
ApoI r'AATT_y
Cuts at: 1493 2237 2546 2576 7258 7484 7636 9745 1493
Size: 744 309 30 4682 226 152 2109 1697
Fragments arranged by size:
4682 2109 1697 744 309 226 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4340 8503 160
Size: 872 3308 4163 1606
Fragments arranged by size:
4163 3308 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9949
AvaI C'yCGr_G
Cuts at: 1870 1943 3393 3512 4805 5143 5183 5262 5409
Size: 73 1450 119 1293 338 40 79 147
Cuts at: 5409 5987 6014 6118 6143 6195 7274 1870
Size: 578 27 104 25 52 1079 4545
Fragments arranged by size:
4545 1450 1293 1079 578 338 147 119
104 79 73 52 40 27 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4853 4886 4853
Size: 33 9916
BamHI G'GATC_C
Cuts at: 7279 7646 7279
Size: 367 9582
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2816 3398 4039 4277 4719
Size: 325 217 627 1029 582 641 238 442
Cuts at: 4719 5212 5309 5405 5653 6800 7270 8725 618
Size: 493 97 96 248 1147 470 1455 1842
Fragments arranged by size:
1842 1455 1147 1029 641 627 582 493
470 442 325 248 238 217 97 96
BanII G_rGCy'C
Cuts at: 1391 2386 2778 2941 3081 3162 4828 4956 5177
Size: 995 392 163 140 81 1666 128 221
Cuts at: 5177 5263 5763 6056 6498 7173 7268 1391
Size: 86 500 293 442 675 95 4072
Fragments arranged by size:
4072 1666 995 675 500 442 392 293
221 163 140 128 95 86 81
BbeI G_GCGC'C
Cuts at: 3402 4043 4281 5216 5313 3402
Size: 641 238 935 97 8038
Fragments arranged by size:
8038 935 641 238 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 3710 4001 4984 5291 5435 6132
Size: 971 531 1280 291 983 307 144 697
Cuts at: 6132 6667 928
Size: 535 4210
Fragments arranged by size:
4210 1280 983 971 697 535 531 307
291 144
BbvCI CC'TCA_GC
Cuts at: 5398 5398
Size: 9949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5878 5912 8140 8174 1903
Size: 34 3941 34 2228 34 3678
Fragments arranged by size:
3941 3678 2228 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4854 7841 9051 4854
Size: 2987 1210 5752
Fragments arranged by size:
5752 2987 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2299 4538 5597
Size: 107 122 71 631 605 627 2239 1059
Cuts at: 5597 5669 6113 6744 8560 136
Size: 72 444 631 1816 1525
Fragments arranged by size:
2239 1816 1525 1059 631 631 627 605
444 122 107 72 71
BglII A'GATC_T
Cuts at: 9944 9944
Size: 9949
BlpI GC'TnA_GC
Cuts at: 5537 5604 6050 7366 5537
Size: 67 446 1316 8120
Fragments arranged by size:
8120 1316 446 67
Bme1580I G_kGCm'C
Cuts at: 1790 4077 4828 5263 5454 7128 8010 9166 1790
Size: 2287 751 435 191 1674 882 1156 2573
Fragments arranged by size:
2573 2287 1674 1156 882 751 435 191
BmgBI CAC'GTC
Cuts at: 1318 3424 4696 5320 6040 6616 6709 1318
Size: 2106 1272 624 720 576 93 4558
Fragments arranged by size:
4558 2106 1272 720 624 576 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2093 3544 5326
Size: 416 53 83 399 24 676 1451 1782
Cuts at: 5326 6174 6306 7680 8638 442
Size: 848 132 1374 958 1753
Fragments arranged by size:
1782 1753 1451 1374 958 848 676 416
399 132 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4000 5191 5413 6538 4000
Size: 1191 222 1125 7411
Fragments arranged by size:
7411 1191 1125 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2462 3065 3122 4279 4283 4418 4574
Size: 1088 135 603 57 1157 4 135 156
Cuts at: 4574 4970 5161 5246 5340 5546 5824 7146 8609
Size: 396 191 85 94 206 278 1322 1463
Cuts at: 8609 1239
Size: 2579
Fragments arranged by size:
2579 1463 1322 1157 1088 603 396 278
206 191 156 135 135 94 85 57
4
Bpu10I CC'TnA_GC
Cuts at: 3161 5398 5966 3161
Size: 2237 568 7144
Fragments arranged by size:
7144 2237 568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 8074 8939 9237 8074
Size: 865 298 8786
Fragments arranged by size:
8786 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2677 3234 4843 5028 8612 882
Size: 617 1178 557 1609 185 3584 2219
Fragments arranged by size:
3584 2219 1609 1178 617 557 185
BsaAI yAC'GTr
Cuts at: 493 1204 6832 7246 7740 493
Size: 711 5628 414 494 2702
Fragments arranged by size:
5628 2702 711 494 414
BsaBI GATnn'nnATC
Cuts at: 9943 9943
Size: 9949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2691 3399 4040 4278
Size: 53 83 186 936 1158 708 641 238
Cuts at: 4278 4868 5213 5310 6255 7756 8138 8773 275
Size: 590 345 97 945 1501 382 635 1451
Fragments arranged by size:
1501 1451 1158 945 936 708 641 635
590 382 345 238 186 97 83 53
BsaWI w'CCGG_w
Cuts at: 2196 2643 4734 5487 6779 8382 8780 9054 9201
Size: 447 2091 753 1292 1603 398 274 147
Cuts at: 9201 9612 2196
Size: 411 2533
Fragments arranged by size:
2533 2091 1603 1292 753 447 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3188 3218 3287 3317 3671
Size: 30 165 30 1785 30 69 30 354
Cuts at: 3671 3701 4012 4042 4547 4577 4822 4852 4979
Size: 30 311 30 505 30 245 30 127
Cuts at: 4979 5009 5141 5171 5690 5720 5840 5870 6194
Size: 30 132 30 519 30 120 30 324
Cuts at: 6194 6224 6761 6791 1178
Size: 30 537 30 4336
Fragments arranged by size:
4336 1785 537 519 505 354 324 311
245 165 132 127 120 69 30 30
30 30 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2916 3212 3479 3482 4005 5539 5673 5738
Size: 1730 296 267 3 523 1534 134 65
Cuts at: 5738 5864 5885 5902 5971 6410 7204 1186
Size: 126 21 17 69 439 794 3931
Fragments arranged by size:
3931 1730 1534 794 523 439 296 267
134 126 69 65 21 17 3
BseYI C'CCAG_C
Cuts at: 4063 5237 5469 7109 9152 9648 4063
Size: 1174 232 1640 2043 496 4364
Fragments arranged by size:
4364 2043 1640 1174 496 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 3884 5355 5441 6434 6901 6956
Size: 573 633 1515 1471 86 993 467 55
Cuts at: 6956 7137 1163
Size: 181 3975
Fragments arranged by size:
3975 1515 1471 993 633 573 467 181
86 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2277 2303 5126 6410 7399 8160
Size: 275 186 276 26 2823 1284 989 761
Cuts at: 8160 9188 1540
Size: 1028 2301
Fragments arranged by size:
2823 2301 1284 1028 989 761 276 275
186 26
BsiHKAI G_wGCw'C
Cuts at: 2250 2778 3081 3162 4188 4226 4956 5177 5622
Size: 528 303 81 1026 38 730 221 445
Cuts at: 5622 6498 7128 8010 8095 9166 2250
Size: 876 630 882 85 1071 3033
Fragments arranged by size:
3033 1071 1026 882 876 730 630 528
445 303 221 85 81 38
BsmI GAATG_Cn'
Cuts at: 7460 7553 7460
Size: 93 9856
BsmBI CGTCTCn'nnnn_
Cuts at: 5914 5914
Size: 9949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2386 2778 2941 3081 3162 4077
Size: 399 460 136 392 163 140 81 915
Cuts at: 4077 4188 4226 4828 4956 5177 5263 5454 5622
Size: 111 38 602 128 221 86 191 168
Cuts at: 5622 5763 6056 6498 7128 7173 7268 8010 8095
Size: 141 293 442 630 45 95 742 85
Cuts at: 8095 9166 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 915 742 630 602 460 442
399 392 293 221 191 168 163 141
140 136 128 111 95 86 85 81
45 38
BspEI T'CCGG_A
Cuts at: 2196 2643 4734 5487 9612 2196
Size: 447 2091 753 4125 2533
Fragments arranged by size:
4125 2533 2091 753 447
BspHI T'CATG_A
Cuts at: 7838 9568 7838
Size: 1730 8219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 3363 5236 5814 6951 7304 844
Size: 1827 692 1873 578 1137 353 3489
Fragments arranged by size:
3489 1873 1827 1137 692 578 353
BsrBI CCG'CTC
Cuts at: 1394 1823 2299 3633 4267 5536 6174 6406 7008
Size: 429 476 1334 634 1269 638 232 602
Cuts at: 7008 7836 1394
Size: 828 3507
Fragments arranged by size:
3507 1334 1269 828 638 634 602 476
429 232
BsrDI GCAATG_nn'
Cuts at: 65 1248 8444 8618 65
Size: 1183 7196 174 1396
Fragments arranged by size:
7196 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2716 2943 5215 5323 5656 5925 6045
Size: 110 906 227 2272 108 333 269 120
Cuts at: 6045 6558 8593 8780 1700
Size: 513 2035 187 2869
Fragments arranged by size:
2869 2272 2035 906 513 333 269 227
187 120 110 108
BsrGI T'GTAC_A
Cuts at: 96 3747 5637 96
Size: 3651 1890 4408
Fragments arranged by size:
4408 3651 1890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9949
BssSI C'ACGA_G
Cuts at: 8009 9021 8009
Size: 1012 8937
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 6001 794
Size: 1529 3678 4742
Fragments arranged by size:
4742 3678 1529
BstBI TT'CG_AA
Cuts at: 2007 8753 2007
Size: 6746 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9949
BstXI CCAn_nnnn'nTGG
Cuts at: 6709 6709
Size: 9949
BstYI r'GATC_y
Cuts at: 1972 2281 5478 6429 7080 7279 7646 8031 8048
Size: 309 3197 951 651 199 367 385 17
Cuts at: 8048 9500 9586 9598 9944 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3197 1977 1452 951 651 385 367 346
309 199 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6057 6057
Size: 9949
BtgI C'CryG_G
Cuts at: 513 1065 2022 2145 2694 3000 3189 3223 4317
Size: 552 957 123 549 306 189 34 1094
Cuts at: 4317 4605 4911 5254 5293 5363 5712 513
Size: 288 306 343 39 70 349 4750
Fragments arranged by size:
4750 1094 957 552 549 349 343 306
306 288 189 123 70 39 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2590 6667 487
Size: 683 56 1364 4077 3769
Fragments arranged by size:
4077 3769 1364 683 56
BtsI GCAGTG_nn'
Cuts at: 2259 2525 3896 4112 4213 4547 5350 6884 7465
Size: 266 1371 216 101 334 803 1534 581
Cuts at: 7465 8259 8279 2259
Size: 794 20 3929
Fragments arranged by size:
3929 1534 1371 803 794 581 334 266
216 101 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9914
DraI TTT'AAA
Cuts at: 7253 7253
Size: 9949
DraIII CAC_nnn'GTG
Cuts at: 2254 2323 2254
Size: 69 9880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2669 2696 3530 8956 809
Size: 507 1353 27 834 5426 1802
Fragments arranged by size:
5426 1802 1353 834 507 27
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2300 3330
Size: 54 1425 50 275 18 195 275 1030
Cuts at: 3330 3543 3672 4314 4539 4572 5217 5670 5923
Size: 213 129 642 225 33 645 453 253
Cuts at: 5923 6631 6918 7396 8285 9914 8
Size: 708 287 478 889 1629 43
Fragments arranged by size:
1629 1425 1030 889 708 645 642 478
453 287 275 275 253 225 213 195
129 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2300 7396 1537
Size: 275 488 5096 4090
Fragments arranged by size:
5096 4090 488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 2393 2490 4094 5012 7879 1353
Size: 885 155 97 1604 918 2867 3423
Fragments arranged by size:
3423 2867 1604 918 885 155 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5489 5698 7043 7061 8536 8910
Size: 1064 138 3900 209 1345 18 1475 374
Cuts at: 8910 9056 387
Size: 146 1280
Fragments arranged by size:
3900 1475 1345 1280 1064 374 209 146
138 18
EcoICRI GAG'CTC
Cuts at: 2776 3079 3160 4954 5175 6496 2776
Size: 303 81 1794 221 1321 6229
Fragments arranged by size:
6229 1794 1321 303 221 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2357 2462 3065 3113 3122 3364
Size: 393 695 30 105 603 48 9 242
Cuts at: 3364 3719 4160 4279 4283 4418 4574 4804 4970
Size: 355 441 119 4 135 156 230 166
Cuts at: 4970 5161 5246 5340 5546 5824 6593 7146 8012
Size: 191 85 94 206 278 769 553 866
Cuts at: 8012 8609 9396 9664 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 866 787 769 695 603 597 553
441 393 355 278 268 242 230 206
191 166 156 135 119 105 94 85
48 30 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2861 3569 4020 4400 5821 2861
Size: 708 451 380 1421 6989
Fragments arranged by size:
6989 1421 708 451 380
EcoO109I rG'GnC_Cy
Cuts at: 2374 2455 4025 4049 4405 4765 4824 5118 5259
Size: 81 1570 24 356 360 59 294 141
Cuts at: 5259 6019 6084 6149 6898 2374
Size: 760 65 65 749 5425
Fragments arranged by size:
5425 1570 760 749 360 356 294 141
81 65 65 59 24
EcoRI G'AATT_C
Cuts at: 2546 7258 7636 2546
Size: 4712 378 4859
Fragments arranged by size:
4859 4712 378
EcoRV GAT'ATC
Cuts at: 7024 7024
Size: 9949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4834 4834
Size: 9949
FseI GG_CCGG'CC
Cuts at: 5929 5929
Size: 9949
FspI TGC'GCA
Cuts at: 3407 8455 3407
Size: 5048 4901
FspAI rTGC'GCAy
Cuts at: 3407 3407
Size: 9949
HaeII r_GCGC'y
Cuts at: 1678 2838 3365 3402 4043 4281 4490 5216 5313
Size: 1160 527 37 641 238 209 726 97
Cuts at: 5313 5611 5792 6739 7207 9096 1678
Size: 298 181 947 468 1889 2531
Fragments arranged by size:
2531 1889 1160 947 726 641 527 468
298 238 209 181 97 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2614 2646 3008
Size: 32 135 32 514 32 496 32 362
Cuts at: 3008 3040 3413 3445 4547 4579 4822 4854 4892
Size: 32 373 32 1102 32 243 32 38
Cuts at: 4892 4924 5141 5173 5840 5872 6380 6412 6743
Size: 32 217 32 667 32 508 32 331
Cuts at: 6743 6775 8590 8622 8664 8696 1373
Size: 32 1815 32 42 32 2626
Fragments arranged by size:
2626 1815 1102 667 514 508 496 373
362 331 243 217 135 42 38 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 7293 7539 669
Size: 988 281 1248 4107 246 3079
Fragments arranged by size:
4107 3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5084 6794 748
Size: 4336 1710 3903
Fragments arranged by size:
4336 3903 1710
HpaI GTT'AAC
Cuts at: 7539 7539
Size: 9949
KasI G'GCGC_C
Cuts at: 3398 4039 4277 5212 5309 3398
Size: 641 238 935 97 8038
Fragments arranged by size:
8038 935 641 238 97
KpnI G_GTAC'C
Cuts at: 7274 7274
Size: 9949
MfeI C'AATT_G
Cuts at: 7548 7548
Size: 9949
MluI A'CGCG_T
Cuts at: 3773 9825 3773
Size: 6052 3897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 5022 5721 5751 6237 6451 7035 7128
Size: 1137 2580 699 30 486 214 584 93
Cuts at: 7128 9063 9247 1305
Size: 1935 184 2007
Fragments arranged by size:
2580 2007 1935 1137 699 584 486 214
184 93 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 3545 4574 6920 10
Size: 54 1425 343 195 1518 1029 2346 3039
Fragments arranged by size:
3039 2346 1518 1425 1029 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 3413 3776 4419 4703 6692 6914
Size: 648 1032 1215 363 643 284 1989 222
Cuts at: 6914 7907 8266 8425 518
Size: 993 359 159 2042
Fragments arranged by size:
2042 1989 1215 1032 993 648 643 363
359 284 222 159
MspA1I CmG'CkG
Cuts at: 1606 2147 2305 2734 4002 4219 4419 4607 5402
Size: 541 158 429 1268 217 200 188 795
Cuts at: 5402 5511 6723 6847 7078 7198 7365 8042 9190
Size: 109 1212 124 231 120 167 677 1148
Cuts at: 9190 9435 1606
Size: 245 2120
Fragments arranged by size:
2120 1268 1212 1148 795 677 541 429
245 231 217 200 188 167 158 124
120 109
NaeI GCC'GGC
Cuts at: 2718 5217 5325 5658 5927 6047 6560 2718
Size: 2499 108 333 269 120 513 6107
Fragments arranged by size:
6107 2499 513 333 269 120 108
NarI GG'CG_CC
Cuts at: 3399 4040 4278 5213 5310 3399
Size: 641 238 935 97 8038
Fragments arranged by size:
8038 935 641 238 97
NcoI C'CATG_G
Cuts at: 513 1065 2022 3000 5293 513
Size: 552 957 978 2293 5169
Fragments arranged by size:
5169 2293 978 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9949
NgoMIV G'CCGG_C
Cuts at: 2716 5215 5323 5656 5925 6045 6558 2716
Size: 2499 108 333 269 120 513 6107
Fragments arranged by size:
6107 2499 513 333 269 120 108
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9949
NotI GC'GGCC_GC
Cuts at: 2300 7396 2300
Size: 5096 4853
NruI TCG'CGA
Cuts at: 3842 3842
Size: 9949
NspI r_CATG'y
Cuts at: 1132 1229 2851 3009 5195 5569 7307 8852 1132
Size: 97 1622 158 2186 374 1738 1545 2229
Fragments arranged by size:
2229 2186 1738 1622 1545 374 158 97
PasI CC'CwG_GG
Cuts at: 1348 5464 5765 6023 1348
Size: 4116 301 258 5274
Fragments arranged by size:
5274 4116 301 258
PciI A'CATG_T
Cuts at: 2847 8848 2847
Size: 6001 3948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 3001 3088 3145 3556 5067 5338 1108
Size: 1752 141 87 57 411 1511 271 5719
Fragments arranged by size:
5719 1752 1511 411 271 141 87 57
PfoI T'CCnGG_A
Cuts at: 5356 5356
Size: 9949
PmeI GTTT'AAAC
Cuts at: 7253 7253
Size: 9949
PmlI CAC'GTG
Cuts at: 6832 6832
Size: 9949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 8018 8050 9531 9563 3287
Size: 32 4699 32 1481 32 3673
Fragments arranged by size:
4699 3673 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 5118 6019 6084 6149 2374
Size: 2744 901 65 65 6174
Fragments arranged by size:
6174 2744 901 65 65
PshAI GACnn'nnGTC
Cuts at: 1613 3170 3710 1613
Size: 1557 540 7852
Fragments arranged by size:
7852 1557 540
PsiI TTA'TAA
Cuts at: 7519 7519
Size: 9949
PspOMI G'GGCC_C
Cuts at: 4824 5259 4824
Size: 435 9514
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6524 6556 6524
Size: 32 9917
PstI C_TGCA'G
Cuts at: 830 2116 2520 2682 3360 3861 4048 4107 4457
Size: 1286 404 162 678 501 187 59 350
Cuts at: 4457 4900 5157 5577 5997 6006 6330 6879 7301
Size: 443 257 420 420 9 324 549 422
Cuts at: 7301 830
Size: 3478
Fragments arranged by size:
3478 1286 678 549 501 443 422 420
420 404 350 324 257 187 162 59
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9949
PvuII CAG'CTG
Cuts at: 4219 5402 5511 4219
Size: 1183 109 8657
Fragments arranged by size:
8657 1183 109
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 9949
SacI G_AGCT'C
Cuts at: 2778 3081 3162 4956 5177 6498 2778
Size: 303 81 1794 221 1321 6229
Fragments arranged by size:
6229 1794 1321 303 221 81
SacII CC_GC'GG
Cuts at: 2148 4608 2148
Size: 2460 7489
SalI G'TCGA_C
Cuts at: 1936 7291 1936
Size: 5355 4594
SanDI GG'GwC_CC
Cuts at: 5118 6019 6084 6149 5118
Size: 901 65 65 8918
Fragments arranged by size:
8918 901 65 65
SapI GCTCTTCn'nnn_
Cuts at: 2238 2490 4094 2238
Size: 252 1604 8093
Fragments arranged by size:
8093 1604 252
SbfI CC_TGCA'GG
Cuts at: 2682 3360 4048 5577 7301 2682
Size: 678 688 1529 1724 5330
Fragments arranged by size:
5330 1724 1529 688 678
ScaI AGT'ACT
Cuts at: 4834 8197 9909 4834
Size: 3363 1712 4874
Fragments arranged by size:
4874 3363 1712
SexAI A'CCwGG_T
Cuts at: 3528 6864 3528
Size: 3336 6613
SfcI C'TryA_G
Cuts at: 826 1045 2112 2516 2678 3307 3356 3857 4044
Size: 219 1067 404 162 629 49 501 187
Cuts at: 4044 4103 4453 4591 4896 5153 5573 5818 5993
Size: 59 350 138 305 257 420 245 175
Cuts at: 5993 6002 6326 6526 6875 7297 8432 9113 9304
Size: 9 324 200 349 422 1135 681 191
Cuts at: 9304 826
Size: 1471
Fragments arranged by size:
1471 1135 1067 681 629 501 422 420
404 350 349 324 305 257 245 219
200 191 187 175 162 138 59 49
9
SfoI GGC'GCC
Cuts at: 3400 4041 4279 5214 5311 3400
Size: 641 238 935 97 8038
Fragments arranged by size:
8038 935 641 238 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9949
SmaI CCC'GGG
Cuts at: 1872 5185 5264 5411 7276 1872
Size: 3313 79 147 1865 4545
Fragments arranged by size:
4545 3313 1865 147 79
SmlI C'TyrA_G
Cuts at: 820 1017 1943 8053 8954 9216 820
Size: 197 926 6110 901 262 1553
Fragments arranged by size:
6110 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7246 7740 493
Size: 6753 494 2702
Fragments arranged by size:
6753 2702 494
SpeI A'CTAG_T
Cuts at: 152 4835 152
Size: 4683 5266
SphI G_CATG'C
Cuts at: 1132 7307 1132
Size: 6175 3774
SspI AAT'ATT
Cuts at: 5 52 7873 5
Size: 47 7821 2081
Fragments arranged by size:
7821 2081 47
StuI AGG'CCT
Cuts at: 1517 3277 3863 5136 5396 6609 1517
Size: 1760 586 1273 260 1213 4857
Fragments arranged by size:
4857 1760 1273 1213 586 260
StyI C'CwwG_G
Cuts at: 513 1065 2022 2812 3000 3144 3333 3690 5205
Size: 552 957 790 188 144 189 357 1515
Cuts at: 5205 5293 6702 7388 513
Size: 88 1409 686 3074
Fragments arranged by size:
3074 1515 1409 957 790 686 552 357
189 188 144 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2834 3563 6461 7976 7993 8146 8331
Size: 1255 1254 729 2898 1515 17 153 185
Cuts at: 8331 8776 325
Size: 445 1498
Fragments arranged by size:
2898 1515 1498 1255 1254 729 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3747 4671
Size: 274 80 33 51 1443 624 1146 924
Cuts at: 4671 4832 5637 8195 9907 96
Size: 161 805 2558 1712 138
Fragments arranged by size:
2558 1712 1443 1146 924 805 624 274
161 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 4012 4044 4205 4237 5398 5430 6842
Size: 32 792 32 161 32 1161 32 1412
Cuts at: 6842 6874 7118 7150 9909 9941 3188
Size: 32 244 32 2759 32 3196
Fragments arranged by size:
3196 2759 1412 1161 792 244 161 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2510 2650 5122 1316
Size: 1194 140 2472 6143
Fragments arranged by size:
6143 2472 1194 140
XbaI T'CTAG_A
Cuts at: 7285 7285
Size: 9949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3553 4456 4534 4885 5068 6706 7117 3553
Size: 903 78 351 183 1638 411 6385
Fragments arranged by size:
6385 1638 903 411 351 183 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9949
XmaI C'CCGG_G
Cuts at: 1870 5183 5262 5409 7274 1870
Size: 3313 79 147 1865 4545
Fragments arranged by size:
4545 3313 1865 147 79
XmnI GAAnn'nnTTC
Cuts at: 2427 2550 3218 8078 2427
Size: 123 668 4860 4298
Fragments arranged by size:
4860 4298 668 123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4869 7757 276
Size: 53 83 186 936 3335 2888 2468
Fragments arranged by size:
3335 2888 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I NsiI PacI PspXI
SfiI SrfI SwaI