Restriction map for
pFN21ASDB0014
(Circular) (Six-base) MAPSORT of: pFN21ASDB0014.seq Check: 6400 from: 1 to: 10609
pFN21ASDB0014 10609 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4528 4830 5848 6010 6610 7561 4528
Size: 302 1018 162 600 951 7576
Fragments arranged by size:
7576 1018 951 600 302 162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4368 8419 278
Size: 53 83 186 936 2832 4051 2468
Fragments arranged by size:
4051 2832 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7952 1937
Size: 6015 4594
Acc65I G'GTAC_C
Cuts at: 7930 7930
Size: 10609
AclI AA'CG_TT
Cuts at: 1563 8736 9109 1563
Size: 7173 373 3063
Fragments arranged by size:
7173 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2436 2906 3047 3728 4373 4502 5558 6899
Size: 804 470 141 681 645 129 1056 1341
Cuts at: 6899 7067 7298 7574 7658 8672 10056 10324 1632
Size: 168 231 276 84 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1056 1014 804 681 645
470 276 268 231 168 141 129 84
AfeI AGC'GCT
Cuts at: 4811 6836 4811
Size: 2025 8584
AflII C'TTAA_G
Cuts at: 820 1017 7391 820
Size: 197 6374 4038
Fragments arranged by size:
6374 4038 197
AflIII A'CryG_T
Cuts at: 1203 2941 2973 9508 10485 1203
Size: 1738 32 6535 977 1327
Fragments arranged by size:
6535 1738 1327 977 32
AgeI A'CCGG_T
Cuts at: 9440 9440
Size: 10609
AhdI GACnn_n'nnGTC
Cuts at: 3109 5383 5905 9338 3109
Size: 2274 522 3433 4380
Fragments arranged by size:
4380 3433 2274 522
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4626 7547 1184
Size: 3442 2921 4246
Fragments arranged by size:
4246 3442 2921
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2826 2858 3605 3637 2826
Size: 32 747 32 9798
Fragments arranged by size:
9798 747 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2593 2656 4822 4906 5158 5686 5791 5968 5995
Size: 63 2166 84 252 528 105 177 27
Cuts at: 5995 6286 6370 7369 8352 9924 2593
Size: 291 84 999 983 1572 3278
Fragments arranged by size:
3278 2166 1572 999 983 528 291 252
177 105 84 84 63 27
ApaI G_GGCC'C
Cuts at: 4735 6475 6610 4735
Size: 1740 135 8734
Fragments arranged by size:
8734 1740 135
ApaLI G'TGCA_C
Cuts at: 2536 8666 9822 2536
Size: 6130 1156 3323
Fragments arranged by size:
6130 3323 1156
ApoI r'AATT_y
Cuts at: 1493 2732 6037 7918 8144 8296 10405 1493
Size: 1239 3305 1881 226 152 2109 1697
Fragments arranged by size:
3305 2109 1881 1697 1239 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9163 160
Size: 872 8131 1606
Fragments arranged by size:
8131 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10609
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 3586 3920 4115 5666 6435 6468
Size: 73 1011 632 334 195 1551 769 33
Cuts at: 6468 7457 7934 1870
Size: 989 477 4545
Fragments arranged by size:
4545 1551 1011 989 769 632 477 334
195 73 33
BamHI G'GATC_C
Cuts at: 3369 3790 7939 8306 3369
Size: 421 4149 367 5672
Fragments arranged by size:
5672 4149 421 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3353 3674 6156 7835
Size: 325 217 627 335 1231 321 2482 1679
Cuts at: 7835 7930 9385 618
Size: 95 1455 1842
Fragments arranged by size:
2482 1842 1679 1455 1231 627 335 325
321 217 95
BanII G_rGCy'C
Cuts at: 1391 2289 2349 4269 4728 4735 5223 5244 5277
Size: 898 60 1920 459 7 488 21 33
Cuts at: 5277 5505 6475 6557 6610 7177 7928 1391
Size: 228 970 82 53 567 751 4072
Fragments arranged by size:
4072 1920 970 898 751 567 488 459
228 82 60 53 33 21 7
BbeI G_GCGC'C
Cuts at: 3357 7839 3357
Size: 4482 6127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2894 3073 4153 4297 5022 5171 5382
Size: 971 995 179 1080 144 725 149 211
Cuts at: 5382 5549 6266 6389 6641 928
Size: 167 717 123 252 4896
Fragments arranged by size:
4896 1080 995 971 725 717 252 211
179 167 149 144 123
BbvCI CC'TCA_GC
Cuts at: 4004 4719 5201 6171 6423 7884 4004
Size: 715 482 970 252 1461 6729
Fragments arranged by size:
6729 1461 970 715 482 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2084 2118 4691 4725 8800 8834 1903
Size: 34 147 34 2573 34 4075 34 3678
Fragments arranged by size:
4075 3678 2573 147 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8501 9711 8501
Size: 1210 9399
BclI T'GATC_A
Cuts at: 1548 5106 7263 1548
Size: 3558 2157 4894
Fragments arranged by size:
4894 3558 2157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 3266 3588
Size: 107 122 71 631 605 427 1167 322
Cuts at: 3588 4140 5288 6119 6719 7460 7622 7685 9220
Size: 552 1148 831 600 741 162 63 1535
Cuts at: 9220 136
Size: 1525
Fragments arranged by size:
1535 1525 1167 1148 831 741 631 605
600 552 427 322 162 122 107 71
63
BglII A'GATC_T
Cuts at: 3393 4767 5358 10604 3393
Size: 1374 591 5246 3398
Fragments arranged by size:
5246 3398 1374 591
BlpI GC'TnA_GC
Cuts at: 5996 8026 5996
Size: 2030 8579
Bme1580I G_kGCm'C
Cuts at: 1790 2540 3677 4735 6475 6610 7421 7815 8670
Size: 750 1137 1058 1740 135 811 394 855
Cuts at: 8670 9826 1790
Size: 1156 2573
Fragments arranged by size:
2573 1740 1156 1137 1058 855 811 750
394 135
BmgBI CAC'GTC
Cuts at: 1318 2944 1318
Size: 1626 8983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 6718 8340
Size: 416 53 83 399 24 690 4611 1622
Cuts at: 8340 9298 442
Size: 958 1753
Fragments arranged by size:
4611 1753 1622 958 690 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10609
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 5206 3206
Size: 2000 8609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2571 2663 2696 2895 3503 4283 4355 4442
Size: 1332 92 33 199 608 780 72 87
Cuts at: 4442 4673 4898 4934 4970 4991 5180 5201 5207
Size: 231 225 36 36 21 189 21 6
Cuts at: 5207 5261 5354 5402 5570 5657 5708 5960 6077
Size: 54 93 48 168 87 51 252 117
Cuts at: 6077 6107 6176 6224 6275 6359 6497 6527 6611
Size: 30 69 48 51 84 138 30 84
Cuts at: 6611 6674 6836 6887 6908 7223 7244 7256 7346
Size: 63 162 51 21 315 21 12 90
Cuts at: 7346 7499 7679 7742 9269 1239
Size: 153 180 63 1527 2579
Fragments arranged by size:
2579 1527 1332 780 608 315 252 231
225 199 189 180 168 162 153 138
117 93 92 90 87 87 84 84
72 69 63 63 54 51 51 51
48 48 36 36 33 30 30 21
21 21 21 12 6
Bpu10I CC'TnA_GC
Cuts at: 4004 4719 5201 5504 6002 6171 6212 6423 7784
Size: 715 482 303 498 169 41 211 1361
Cuts at: 7784 7884 4004
Size: 100 6729
Fragments arranged by size:
6729 1361 715 498 482 303 211 169
100 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2309 4886 5069 5228 5315 5999 7631 8734
Size: 165 2577 183 159 87 684 1632 1103
Cuts at: 8734 9599 9897 2144
Size: 865 298 2856
Fragments arranged by size:
2856 2577 1632 1103 865 684 298 183
165 159 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2978 3902 4341 6440 7493 9272 882
Size: 617 1479 924 439 2099 1053 1779 2219
Fragments arranged by size:
2219 2099 1779 1479 1053 924 617 439
BsaAI yAC'GTr
Cuts at: 493 1204 2292 2602 7906 8400 493
Size: 711 1088 310 5304 494 2702
Fragments arranged by size:
5304 2702 1088 711 494 310
BsaBI GATnn'nnATC
Cuts at: 2046 10603 2046
Size: 8557 2052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2606 3110 3354 4365
Size: 53 83 186 936 1073 504 244 1011
Cuts at: 4365 7198 7728 7836 8416 8798 9433 275
Size: 2833 530 108 580 382 635 1451
Fragments arranged by size:
2833 1451 1073 1011 936 635 580 530
504 382 244 186 108 83 53
BsaWI w'CCGG_w
Cuts at: 9042 9440 9714 9861 10272 9042
Size: 398 274 147 411 9379
Fragments arranged by size:
9379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2702 2732 3605 3635 5492
Size: 30 165 30 1299 30 873 30 1857
Cuts at: 5492 5522 5805 5835 7427 7457 1178
Size: 30 283 30 1592 30 4330
Fragments arranged by size:
4330 1857 1592 1299 873 283 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2707 3038 3506 3626 4352 4355 4568
Size: 994 527 331 468 120 726 3 213
Cuts at: 4568 4571 4589 4592 4736 4811 4814 4817 4820
Size: 3 18 3 144 75 3 3 3
Cuts at: 4820 4883 4895 4898 4901 4967 4970 5069 5072
Size: 63 12 3 3 66 3 99 3
Cuts at: 5072 5153 5282 5285 5567 5570 5633 5993 6074
Size: 81 129 3 282 3 63 360 81
Cuts at: 6074 6077 6080 6083 6188 6242 6392 6422 6641
Size: 3 3 3 105 54 150 30 219
Cuts at: 6641 6779 7022 7202 7241 7244 7253 7256 7496
Size: 138 243 180 39 3 9 3 240
Cuts at: 7496 7676 7688 1186
Size: 180 12 4107
Fragments arranged by size:
4107 994 726 527 468 360 331 282
243 240 219 213 180 180 150 144
138 129 120 105 99 81 81 75
66 63 63 54 39 30 18 12
12 9 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 5493 5946 7119 7218 7680 9812 10308 5493
Size: 453 1173 99 462 2132 496 5794
Fragments arranged by size:
5794 2132 1173 496 462 453 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 9472
Fragments arranged by size:
9472 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6418 7273 7743 8059 8820 9848
Size: 275 186 4417 855 470 316 761 1028
Cuts at: 9848 1540
Size: 2301
Fragments arranged by size:
4417 2301 1028 855 761 470 316 275
186
BsiHKAI G_wGCw'C
Cuts at: 2289 2540 2883 4248 4269 4728 5223 5244 5277
Size: 251 343 1365 21 459 495 21 33
Cuts at: 5277 5505 5757 6008 6557 8670 8755 9826 2289
Size: 228 252 251 549 2113 85 1071 3072
Fragments arranged by size:
3072 2113 1365 1071 549 495 459 343
252 251 251 228 85 33 21 21
BsiWI C'GTAC_G
Cuts at: 2598 2598
Size: 10609
BsmI GAATG_Cn'
Cuts at: 3591 7360 8120 8213 3591
Size: 3769 760 93 5987
Fragments arranged by size:
5987 3769 760 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4631 7730 4631
Size: 3099 7510
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2349 2540 2883 3677 4248 4269
Size: 399 499 60 191 343 794 571 21
Cuts at: 4269 4728 4735 5223 5244 5277 5505 5757 6008
Size: 459 7 488 21 33 228 252 251
Cuts at: 6008 6475 6557 6610 7177 7421 7815 7928 8670
Size: 467 82 53 567 244 394 113 742
Cuts at: 8670 8755 9826 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 794 742 571 567 499 488
467 459 399 394 343 252 251 244
228 191 113 85 82 60 53 33
21 21 7
BspEI T'CCGG_A
Cuts at: 10272 10272
Size: 10609
BspHI T'CATG_A
Cuts at: 4248 8498 10228 4248
Size: 4250 1730 4629
Fragments arranged by size:
4629 4250 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 3552 4528 4830 5785 5848 6010 6303
Size: 1565 1143 976 302 955 63 162 293
Cuts at: 6303 6610 6714 6747 7299 7561 7806 7903 7964
Size: 307 104 33 552 262 245 97 61
Cuts at: 7964 844
Size: 3489
Fragments arranged by size:
3489 1565 1143 976 955 552 307 302
293 262 245 162 104 97 63 61
33
BsrBI CCG'CTC
Cuts at: 1394 1823 2647 3094 3292 5698 6511 6733 7129
Size: 429 824 447 198 2406 813 222 396
Cuts at: 7129 7585 8496 1394
Size: 456 911 3507
Fragments arranged by size:
3507 2406 911 824 813 456 447 429
396 222 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 3378 5109 9104 9278 65
Size: 1183 2130 1731 3995 174 1396
Fragments arranged by size:
3995 2130 1731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3383 3743 3896 4061 4316 4545 5985
Size: 110 1573 360 153 165 255 229 1440
Cuts at: 5985 7272 9253 9440 1700
Size: 1287 1981 187 2869
Fragments arranged by size:
2869 1981 1573 1440 1287 360 255 229
187 165 153 110
BsrGI T'GTAC_A
Cuts at: 96 2433 96
Size: 2337 8272
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10609
BssSI C'ACGA_G
Cuts at: 2453 2808 4049 4082 5570 6224 6584 7002 8669
Size: 355 1241 33 1488 654 360 418 1667
Cuts at: 8669 9681 2453
Size: 1012 3381
Fragments arranged by size:
3381 1667 1488 1241 1012 654 418 360
355 33
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2458 2656 4084 4906 5791 794
Size: 1664 198 1428 822 885 5612
Fragments arranged by size:
5612 1664 1428 885 822 198
BstBI TT'CG_AA
Cuts at: 2007 9413 2007
Size: 7406 3203
BstEII G'GTnAC_C
Cuts at: 1184 4921 5845 1184
Size: 3737 924 5948
Fragments arranged by size:
5948 3737 924
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 3994 4156 2113
Size: 1881 162 8566
Fragments arranged by size:
8566 1881 162
BstYI r'GATC_y
Cuts at: 1972 3369 3393 3790 4188 4767 5337 5358 5580
Size: 1397 24 397 398 579 570 21 222
Cuts at: 5580 7011 7939 8306 8691 8708 10160 10246 10258
Size: 1431 928 367 385 17 1452 86 12
Cuts at: 10258 10604 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1431 1397 928 579 570 398
397 385 367 346 222 86 24 21
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2988 5613 6486 7095 513
Size: 552 957 966 2625 873 609 4027
Fragments arranged by size:
4027 2625 966 957 873 609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3793 7198 487
Size: 683 56 2567 3405 3898
Fragments arranged by size:
3898 3405 2567 683 56
BtsI GCAGTG_nn'
Cuts at: 3815 3914 4505 6852 8125 8919 8939 3815
Size: 99 591 2347 1273 794 20 5485
Fragments arranged by size:
5485 2347 1273 794 591 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10574
DraI TTT'AAA
Cuts at: 7913 7913
Size: 10609
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3842 4645 9616 809
Size: 507 2526 803 4971 1802
Fragments arranged by size:
4971 2526 1802 803 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3273 3894 3984
Size: 54 1425 50 275 18 1443 621 90
Cuts at: 3984 4291 4404 4566 4992 5256 5289 5709 5835
Size: 307 113 162 426 264 33 420 126
Cuts at: 5835 6120 6237 6849 6906 7740 8056 8945 10574
Size: 285 117 612 57 834 316 889 1629
Cuts at: 10574 8
Size: 43
Fragments arranged by size:
1629 1443 1425 889 834 621 612 426
420 316 307 285 275 264 162 126
117 113 90 57 54 50 43 33
18
EagI C'GGCC_G
Cuts at: 1537 1812 7740 8056 1537
Size: 275 5928 316 4090
Fragments arranged by size:
5928 4090 316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2412 2441 3015 3464 4517 4758 5046
Size: 819 240 29 574 449 1053 241 288
Cuts at: 5046 5115 5295 5433 6176 6642 7043 7665 7851
Size: 69 180 138 743 466 401 622 186
Cuts at: 7851 8539 1353
Size: 688 3423
Fragments arranged by size:
3423 1053 819 743 688 622 574 466
449 401 288 241 240 186 180 138
69 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2807 4844 5642 6389 6443 6515
Size: 1064 138 1218 2037 798 747 54 72
Cuts at: 6515 9196 9570 9716 387
Size: 2681 374 146 1280
Fragments arranged by size:
2681 2037 1280 1218 1064 798 747 374
146 138 72 54
EcoICRI GAG'CTC
Cuts at: 2287 4267 4726 5221 5242 5275 5503 6555 2287
Size: 1980 459 495 21 33 228 1052 6341
Fragments arranged by size:
6341 1980 1052 495 459 228 33 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2436 2571 2663 2696 2895 2906 3047
Size: 393 804 135 92 33 199 11 141
Cuts at: 3047 3503 3728 4283 4355 4373 4442 4502 4673
Size: 456 225 555 72 18 69 60 171
Cuts at: 4673 4898 4934 4970 4991 5180 5201 5207 5261
Size: 225 36 36 21 189 21 6 54
Cuts at: 5261 5354 5402 5558 5570 5657 5708 5960 6077
Size: 93 48 156 12 87 51 252 117
Cuts at: 6077 6107 6176 6224 6275 6359 6497 6527 6611
Size: 30 69 48 51 84 138 30 84
Cuts at: 6611 6674 6836 6887 6899 6908 7067 7223 7244
Size: 63 162 51 12 9 159 156 21
Cuts at: 7244 7256 7298 7346 7499 7574 7658 7679 7742
Size: 12 42 48 153 75 84 21 63
Cuts at: 7742 8672 9269 10056 10324 1239
Size: 930 597 787 268 1524
Fragments arranged by size:
1524 930 804 787 597 555 456 393
268 252 225 225 199 189 171 162
159 156 156 153 141 138 135 117
93 92 87 84 84 84 75 72
69 69 63 63 60 54 51 51
51 48 48 48 42 36 36 33
30 30 21 21 21 21 18 12
12 12 11 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 3485 3887 4337 4390 5552 5669 6374 7019
Size: 1323 402 450 53 1162 117 705 645
Cuts at: 7019 2162
Size: 5752
Fragments arranged by size:
5752 1323 1162 705 645 450 402 117
53
EcoO109I rG'GnC_Cy
Cuts at: 3581 3924 4453 5428 6199 6208 6472 6544 6607
Size: 343 529 975 771 9 264 72 63
Cuts at: 6607 6736 6883 7795 3581
Size: 129 147 912 6395
Fragments arranged by size:
6395 975 912 771 529 343 264 147
129 72 63 9
EcoRI G'AATT_C
Cuts at: 6037 7918 8296 6037
Size: 1881 378 8350
Fragments arranged by size:
8350 1881 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 6058 2158
Size: 3900 6709
FspI TGC'GCA
Cuts at: 3317 5825 9115 3317
Size: 2508 3290 4811
Fragments arranged by size:
4811 3290 2508
FspAI rTGC'GCAy
Cuts at: 5825 5825
Size: 10609
HaeII r_GCGC'y
Cuts at: 1678 3357 4813 4896 5128 5453 6322 6838 7114
Size: 1679 1456 83 232 325 869 516 276
Cuts at: 7114 7513 7839 9756 1678
Size: 399 326 1917 2531
Fragments arranged by size:
2531 1917 1679 1456 869 516 399 326
325 276 232 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2449 2481 2702 2734 3095
Size: 32 135 32 877 32 221 32 361
Cuts at: 3095 3127 3385 3392 3417 3424 4173 4180 4205
Size: 32 258 7 25 7 749 7 25
Cuts at: 4205 4212 5048 5080 5336 5368 5369 5401 5492
Size: 7 836 32 256 32 1 32 91
Cuts at: 5492 5524 5891 5923 6439 6471 7610 7642 9250
Size: 32 367 32 516 32 1139 32 1608
Cuts at: 9250 9282 9324 9356 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1608 1139 877 836 749 516 367
361 258 256 221 135 91 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 25 25 7
7 7 7 1
HincII GTy'rAC
Cuts at: 669 1657 1938 6703 7953 8199 669
Size: 988 281 4765 1250 246 3079
Fragments arranged by size:
4765 3079 1250 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10609
HpaI GTT'AAC
Cuts at: 8199 8199
Size: 10609
KasI G'GCGC_C
Cuts at: 3353 7835 3353
Size: 4482 6127
KpnI G_GTAC'C
Cuts at: 7934 7934
Size: 10609
MfeI C'AATT_G
Cuts at: 8208 8208
Size: 10609
MluI A'CGCG_T
Cuts at: 10485 10485
Size: 10609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 2752 2883 3885 5445 5922 6675 7517
Size: 849 598 131 1002 1560 477 753 842
Cuts at: 7517 7875 9723 9907 1305
Size: 358 1848 184 2007
Fragments arranged by size:
2007 1848 1560 1002 849 842 753 598
477 358 184 131
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3275 3986 4293 4406 4568
Size: 54 1425 343 1443 711 307 113 162
Cuts at: 4568 4994 5258 5291 5711 10
Size: 426 264 33 420 4908
Fragments arranged by size:
4908 1443 1425 711 426 420 343 307
264 162 113 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 3206 4631 6536 8567 8926 9085
Size: 648 1306 734 1425 1905 2031 359 159
Cuts at: 9085 518
Size: 2042
Fragments arranged by size:
2042 2031 1905 1425 1306 734 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2083 2653 3131 3303 3421 3706 3982 4032
Size: 477 570 478 172 118 285 276 50
Cuts at: 4032 4096 4462 4531 4606 4675 4876 4912 5254
Size: 64 366 69 75 69 201 36 342
Cuts at: 5254 5489 5635 5782 5950 6055 6220 6643 6781
Size: 235 146 147 168 105 165 423 138
Cuts at: 6781 6940 7097 7164 7222 7369 7477 7489 7657
Size: 159 157 67 58 147 108 12 168
Cuts at: 7657 7684 8025 8702 9850 10095 1606
Size: 27 341 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 570 478 477 423 366
342 341 285 276 245 235 201 172
168 168 165 159 157 147 147 146
138 118 108 105 75 69 69 67
64 58 50 36 27 12
NaeI GCC'GGC
Cuts at: 3385 3745 3898 4063 4547 3385
Size: 360 153 165 484 9447
Fragments arranged by size:
9447 484 360 165 153
NarI GG'CG_CC
Cuts at: 3354 7836 3354
Size: 4482 6127
NcoI C'CATG_G
Cuts at: 513 1065 2022 2988 6486 513
Size: 552 957 966 3498 4636
Fragments arranged by size:
4636 3498 966 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10609
NgoMIV G'CCGG_C
Cuts at: 3383 3743 3896 4061 4545 3383
Size: 360 153 165 484 9447
Fragments arranged by size:
9447 484 360 165 153
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10609
NotI GC'GGCC_GC
Cuts at: 8056 8056
Size: 10609
NspI r_CATG'y
Cuts at: 1132 1229 2977 3904 3949 6976 7967 9512 1132
Size: 97 1748 927 45 3027 991 1545 2229
Fragments arranged by size:
3027 2229 1748 1545 991 927 97 45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10609
PciI A'CATG_T
Cuts at: 2973 9508 2973
Size: 6535 4074
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2593 3154 6370 6589 1108
Size: 1485 561 3216 219 5128
Fragments arranged by size:
5128 3216 1485 561 219
PfoI T'CCnGG_A
Cuts at: 2673 2979 3372 3480 4164 4857 4968 5340 5370
Size: 306 393 108 684 693 111 372 30
Cuts at: 5370 5399 5583 6045 2673
Size: 29 184 462 7237
Fragments arranged by size:
7237 693 684 462 393 372 306 184
111 108 30 29
PmeI GTTT'AAAC
Cuts at: 7913 7913
Size: 10609
PmlI CAC'GTG
Cuts at: 2292 2292
Size: 10609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3605 3637 8678 8710 10191 10223 3281
Size: 32 292 32 5041 32 1481 32 3667
Fragments arranged by size:
5041 3667 1481 292 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4453 5428 6199 6544 6736 6883 7795 4453
Size: 975 771 345 192 147 912 7267
Fragments arranged by size:
7267 975 912 771 345 192 147
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10609
PsiI TTA'TAA
Cuts at: 8179 8179
Size: 10609
PspOMI G'GGCC_C
Cuts at: 4731 6471 6606 4731
Size: 1740 135 8734
Fragments arranged by size:
8734 1740 135
PspXI vC'TCGA_Gb
Cuts at: 7457 7457
Size: 10609
PstI C_TGCA'G
Cuts at: 830 2661 3453 3549 4394 4539 4647 4680 4827
Size: 1831 792 96 845 145 108 33 147
Cuts at: 4827 4911 5736 5787 5796 5859 5955 5976 6279
Size: 84 825 51 9 63 96 21 303
Cuts at: 6279 6669 6711 6744 6954 7296 7407 7572 7713
Size: 390 42 33 210 342 111 165 141
Cuts at: 7713 7961 830
Size: 248 3478
Fragments arranged by size:
3478 1831 845 825 792 390 342 303
248 210 165 147 145 141 111 108
96 96 84 63 51 42 33 33
21 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10609
PvuII CAG'CTG
Cuts at: 2653 3131 3421 3706 3982 4096 4606 4675 4876
Size: 478 290 285 276 114 510 69 201
Cuts at: 4876 4912 5254 5489 5635 5782 5950 6055 6643
Size: 36 342 235 146 147 168 105 588
Cuts at: 6643 6781 6940 7222 7369 7477 7489 7657 2653
Size: 138 159 282 147 108 12 168 5605
Fragments arranged by size:
5605 588 510 478 342 290 285 282
276 235 201 168 168 159 147 147
146 138 114 108 105 69 36 12
RsrII CG'GwC_CG
Cuts at: 7539 7539
Size: 10609
SacI G_AGCT'C
Cuts at: 2289 4269 4728 5223 5244 5277 5505 6557 2289
Size: 1980 459 495 21 33 228 1052 6341
Fragments arranged by size:
6341 1980 1052 495 459 228 33 21
SacII CC_GC'GG
Cuts at: 7098 7098
Size: 10609
SalI G'TCGA_C
Cuts at: 1936 7951 1936
Size: 6015 4594
SapI GCTCTTCn'nnn_
Cuts at: 3464 4517 5115 5433 6642 7043 3464
Size: 1053 598 318 1209 401 7030
Fragments arranged by size:
7030 1209 1053 598 401 318
SbfI CC_TGCA'GG
Cuts at: 4827 6711 6744 7961 4827
Size: 1884 33 1217 7475
Fragments arranged by size:
7475 1884 1217 33
ScaI AGT'ACT
Cuts at: 8857 10569 8857
Size: 1712 8897
SexAI A'CCwGG_T
Cuts at: 3888 3888
Size: 10609
SfcI C'TryA_G
Cuts at: 826 1045 2494 2657 3449 3545 4390 4535 4643
Size: 219 1449 163 792 96 845 145 108
Cuts at: 4643 4676 4823 4907 5732 5783 5792 5855 5951
Size: 33 147 84 825 51 9 63 96
Cuts at: 5951 5972 6275 6665 6707 6740 6950 7292 7403
Size: 21 303 390 42 33 210 342 111
Cuts at: 7403 7568 7709 7957 9092 9773 9964 826
Size: 165 141 248 1135 681 191 1471
Fragments arranged by size:
1471 1449 1135 845 825 792 681 390
342 303 248 219 210 191 165 163
147 145 141 111 108 96 96 84
63 51 42 33 33 21 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5288 6719 7685 5288
Size: 1431 966 8212
Fragments arranged by size:
8212 1431 966
SfoI GGC'GCC
Cuts at: 3355 7837 3355
Size: 4482 6127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10609
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10609
SmaI CCC'GGG
Cuts at: 1872 2956 3588 3922 6470 7936 1872
Size: 1084 632 334 2548 1466 4545
Fragments arranged by size:
4545 2548 1466 1084 632 334
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2324 4115 4865 5084 5243
Size: 197 926 216 165 1791 750 219 159
Cuts at: 5243 5330 5666 6014 7391 7457 7646 8713 9614
Size: 87 336 348 1377 66 189 1067 901
Cuts at: 9614 9876 820
Size: 262 1553
Fragments arranged by size:
1791 1553 1377 1067 926 901 750 348
336 262 219 216 197 189 165 159
87 66
SnaBI TAC'GTA
Cuts at: 493 7906 8400 493
Size: 7413 494 2702
Fragments arranged by size:
7413 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10609
SphI G_CATG'C
Cuts at: 1132 6976 7967 1132
Size: 5844 991 3774
Fragments arranged by size:
5844 3774 991
SrfI GCCC'GGGC
Cuts at: 6470 6470
Size: 10609
SspI AAT'ATT
Cuts at: 5 52 8533 5
Size: 47 8481 2081
Fragments arranged by size:
8481 2081 47
StuI AGG'CCT
Cuts at: 1517 2367 2681 3269 4385 7442 1517
Size: 850 314 588 1116 3057 4684
Fragments arranged by size:
4684 3057 1116 850 588 314
StyI C'CwwG_G
Cuts at: 513 1065 2022 2265 2826 2988 3270 3276 4212
Size: 552 957 243 561 162 282 6 936
Cuts at: 4212 4518 4569 4995 5431 5922 6450 6486 6558
Size: 306 51 426 436 491 528 36 72
Cuts at: 6558 7503 8048 513
Size: 945 545 3074
Fragments arranged by size:
3074 957 945 936 561 552 545 528
491 436 426 306 282 243 162 72
51 36 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2110 2529 2906 3185 3692 7556 8636
Size: 1255 530 419 377 279 507 3864 1080
Cuts at: 8636 8653 8806 8991 9436 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3864 1498 1255 1080 530 507 445 419
377 279 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 7434 7602
Size: 274 80 33 51 1443 456 5001 168
Cuts at: 7602 8855 10567 96
Size: 1253 1712 138
Fragments arranged by size:
5001 1712 1443 1253 456 274 168 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 5805 5837 10569 10601 3599
Size: 32 2174 32 4732 32 3607
Fragments arranged by size:
4732 3607 2174 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5843 1316
Size: 4527 6082
XbaI T'CTAG_A
Cuts at: 7945 7945
Size: 10609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2671 3004 3268 3478 4219 5857 6082 6565
Size: 480 333 264 210 741 1638 225 483
Cuts at: 6565 6691 2191
Size: 126 6109
Fragments arranged by size:
6109 1638 741 483 480 333 264 225
210 126
XhoI C'TCGA_G
Cuts at: 1943 4115 5666 7457 1943
Size: 2172 1551 1791 5095
Fragments arranged by size:
5095 2172 1791 1551
XmaI C'CCGG_G
Cuts at: 1870 2954 3586 3920 6468 7934 1870
Size: 1084 632 334 2548 1466 4545
Fragments arranged by size:
4545 2548 1466 1084 632 334
XmnI GAAnn'nnTTC
Cuts at: 6041 8738 6041
Size: 2697 7912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4366 8417 276
Size: 53 83 186 936 2832 4051 2468
Fragments arranged by size:
4051 2832 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I Bsu36I DraIII EcoRV
FseI NruI NsiI PacI PsrI SanDI SwaI