Restriction map for
pFN21ASDB0010
(Circular) (Six-base) MAPSORT of: pFN21ASDB0010.seq Check: 3498 from: 1 to: 6586
pFN21ASDB0010 6586 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4396 278
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3360 3929 1937
Size: 1423 569 4594
Fragments arranged by size:
4594 1423 569
Acc65I G'GTAC_C
Cuts at: 2153 3907 2153
Size: 1754 4832
AclI AA'CG_TT
Cuts at: 1563 4713 5086 1563
Size: 3150 373 3063
Fragments arranged by size:
3150 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 3292 4649 6033 6301 1632
Size: 942 718 1357 1384 268 1917
Fragments arranged by size:
1917 1384 1357 942 718 268
AfeI AGC'GCT
Cuts at: 3134 3467 3134
Size: 333 6253
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6389
AflIII A'CryG_T
Cuts at: 1203 5485 6462 1203
Size: 4282 977 1327
Fragments arranged by size:
4282 1327 977
AgeI A'CCGG_T
Cuts at: 5417 5417
Size: 6586
AhdI GACnn_n'nnGTC
Cuts at: 5315 5315
Size: 6586
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6586
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2196
Size: 32 6554
AlwNI CAG_nnn'CTG
Cuts at: 2168 2971 3040 3491 4329 5901 2168
Size: 803 69 451 838 1572 2853
Fragments arranged by size:
2853 1572 838 803 451 69
ApaI G_GGCC'C
Cuts at: 2124 2361 2531 2124
Size: 237 170 6179
Fragments arranged by size:
6179 237 170
ApaLI G'TGCA_C
Cuts at: 4643 5799 4643
Size: 1156 5430
ApoI r'AATT_y
Cuts at: 1493 3651 3895 4121 4273 6382 1493
Size: 2158 244 226 152 2109 1697
Fragments arranged by size:
2158 2109 1697 244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5140 160
Size: 872 4108 1606
Fragments arranged by size:
4108 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 3261 3911 1870
Size: 73 783 535 650 4545
Fragments arranged by size:
4545 783 650 535 73
BamHI G'GATC_C
Cuts at: 3459 3916 4283 3459
Size: 457 367 5762
Fragments arranged by size:
5762 457 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2787 2976 3907 5362
Size: 325 217 627 366 634 189 931 1455
Cuts at: 5362 618
Size: 1842
Fragments arranged by size:
1842 1455 931 634 627 366 325 217
189
BanII G_rGCy'C
Cuts at: 1391 2124 2227 2361 2531 2961 3644 3751 3905
Size: 733 103 134 170 430 683 107 154
Cuts at: 3905 1391
Size: 4072
Fragments arranged by size:
4072 733 683 430 170 154 134 107
103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2726 3274 928
Size: 971 827 548 4240
Fragments arranged by size:
4240 971 827 548
BbvCI CC'TCA_GC
Cuts at: 2324 2324
Size: 6586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4777 4811 1903
Size: 34 2840 34 3678
Fragments arranged by size:
3678 2840 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2058 3076 4478 5688 2058
Size: 1018 1402 1210 2956
Fragments arranged by size:
2956 1402 1210 1018
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6586
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 3625 5197
Size: 107 122 71 631 605 1036 917 1572
Cuts at: 5197 136
Size: 1525
Fragments arranged by size:
1572 1525 1036 917 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3528 6581 3528
Size: 3053 3533
BlpI GC'TnA_GC
Cuts at: 2847 4003 2847
Size: 1156 5430
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2361 2531 2979 3129 4647 5803 1790
Size: 334 237 170 448 150 1518 1156 2573
Fragments arranged by size:
2573 1518 1156 448 334 237 170 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 4317 5275
Size: 416 53 83 399 24 661 2239 958
Cuts at: 5275 442
Size: 1753
Fragments arranged by size:
2239 1753 958 661 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2154 2154
Size: 6586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2553 3264 3416 3424 3651 3795 5246 1239
Size: 1314 711 152 8 227 144 1451 2579
Fragments arranged by size:
2579 1451 1314 711 227 152 144 8
Bpu10I CC'TnA_GC
Cuts at: 2324 2715 3192 2324
Size: 391 477 5718
Fragments arranged by size:
5718 477 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3029 3041 4711 5576 5874 3029
Size: 12 1670 865 298 3741
Fragments arranged by size:
3741 1670 865 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5249 882
Size: 617 3750 2219
Fragments arranged by size:
3750 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2665 3883 4377 493
Size: 711 1461 1218 494 2702
Fragments arranged by size:
2702 1461 1218 711 494
BsaBI GATnn'nnATC
Cuts at: 6580 6580
Size: 6586
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4393 4775 5410 275
Size: 53 83 186 936 2860 382 635 1451
Fragments arranged by size:
2860 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 2288 5019 5417 5691 5838 6249 2061
Size: 227 2731 398 274 147 411 2398
Fragments arranged by size:
2731 2398 411 398 274 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 3101 3131 3315
Size: 30 165 30 1149 30 519 30 184
Cuts at: 3315 3345 1178
Size: 30 4419
Fragments arranged by size:
4419 1149 519 184 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2389 2501 2573 2603 2969 2990 3339 3342
Size: 1203 112 72 30 366 21 349 3
Cuts at: 3342 3798 1186
Size: 456 3974
Fragments arranged by size:
3974 1203 456 366 349 112 72 30
21 3
BseYI C'CCAG_C
Cuts at: 2312 2971 3074 3154 5789 6285 2312
Size: 659 103 80 2635 496 2613
Fragments arranged by size:
2635 2613 659 496 103 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3035 1163
Size: 573 1299 4714
Fragments arranged by size:
4714 1299 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4036 4797 5825 1540
Size: 275 186 2035 761 1028 2301
Fragments arranged by size:
2301 2035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2961 4647 4732 5803 2961
Size: 1686 85 1071 3744
Fragments arranged by size:
3744 1686 1071 85
BsiWI C'GTAC_G
Cuts at: 2174 2174
Size: 6586
BsmI GAATG_Cn'
Cuts at: 4097 4190 4097
Size: 93 6493
BsmBI CGTCTCn'nnnn_
Cuts at: 3580 3580
Size: 6586
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2227 2361 2531 2961 2979 3129
Size: 399 334 103 134 170 430 18 150
Cuts at: 3129 3644 3751 3905 4647 4732 5803 1391
Size: 515 107 154 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 430 399 334 170
154 150 134 107 103 85 18
BspEI T'CCGG_A
Cuts at: 6249 6249
Size: 6586
BspHI T'CATG_A
Cuts at: 3024 4475 6205 3024
Size: 1451 1730 3405
Fragments arranged by size:
3405 1730 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3941 844
Size: 3097 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4473 1394
Size: 429 2650 3507
Fragments arranged by size:
3507 2650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 3560 5081 5255 65
Size: 1183 999 1313 1521 174 1396
Fragments arranged by size:
1521 1396 1313 1183 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2871 5230 5417 1700
Size: 110 1061 2359 187 2869
Fragments arranged by size:
2869 2359 1061 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6586
BssHII G'CGCG_C
Cuts at: 1925 2092 1925
Size: 167 6419
BssSI C'ACGA_G
Cuts at: 2864 4646 5658 2864
Size: 1782 1012 3792
Fragments arranged by size:
3792 1782 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2983 3491 794
Size: 2189 508 3889
Fragments arranged by size:
3889 2189 508
BstBI TT'CG_AA
Cuts at: 2007 5390 2007
Size: 3383 3203
BstEII G'GTnAC_C
Cuts at: 1184 3226 3413 1184
Size: 2042 187 4357
Fragments arranged by size:
4357 2042 187
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 3610 2521
Size: 1089 5497
BstYI r'GATC_y
Cuts at: 1972 2830 3459 3528 3916 4283 4668 4685 6137
Size: 858 629 69 388 367 385 17 1452
Cuts at: 6137 6223 6235 6581 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 858 629 388 385 367 346
86 69 17 12
BstZ17I GTA'TAC
Cuts at: 3361 3361
Size: 6586
Bsu36I CC'TnA_GG
Cuts at: 2372 3237 2372
Size: 865 5721
BtgI C'CryG_G
Cuts at: 513 1065 2706 513
Size: 552 1641 4393
Fragments arranged by size:
4393 1641 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5847
Fragments arranged by size:
5847 683 56
BtsI GCAGTG_nn'
Cuts at: 2263 4102 4896 4916 2263
Size: 1839 794 20 3933
Fragments arranged by size:
3933 1839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6551
DraI TTT'AAA
Cuts at: 3890 3890
Size: 6586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5593 809
Size: 507 4277 1802
Fragments arranged by size:
4277 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2700 3617
Size: 54 1425 50 275 18 220 650 917
Cuts at: 3617 3741 4033 4922 6551 8
Size: 124 292 889 1629 43
Fragments arranged by size:
1629 1425 917 889 650 292 275 220
124 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4033 1537
Size: 275 2221 4090
Fragments arranged by size:
4090 2221 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 4516 1353
Size: 1197 1966 3423
Fragments arranged by size:
3423 1966 1197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5173 5547 5693 387
Size: 1064 138 3584 374 146 1280
Fragments arranged by size:
3584 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2959 2959
Size: 6586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2553 2574 3264 3292 3416 3424 3651
Size: 393 921 21 690 28 124 8 227
Cuts at: 3651 3795 4649 5246 6033 6301 1239
Size: 144 854 597 787 268 1524
Fragments arranged by size:
1524 921 854 787 690 597 393 268
227 144 124 28 21 8
EcoO109I rG'GnC_Cy
Cuts at: 2120 2121 2212 2348 2357 2527 2528 2722 3626
Size: 1 91 136 9 170 1 194 904
Cuts at: 3626 3772 2120
Size: 146 4934
Fragments arranged by size:
4934 904 194 170 146 136 91 9
1 1
EcoRI G'AATT_C
Cuts at: 3651 3895 4273 3651
Size: 244 378 5964
Fragments arranged by size:
5964 378 244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3869 3869
Size: 6586
FspI TGC'GCA
Cuts at: 5092 5092
Size: 6586
HaeII r_GCGC'y
Cuts at: 1678 2872 3136 3469 5733 1678
Size: 1194 264 333 2264 2531
Fragments arranged by size:
2531 2264 1194 333 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2552 2584 5227
Size: 32 135 32 578 32 370 32 2643
Cuts at: 5227 5259 5301 5333 1373
Size: 32 42 32 2626
Fragments arranged by size:
2643 2626 578 370 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3930 4176 669
Size: 988 281 1992 246 3079
Fragments arranged by size:
3079 1992 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6586
HpaI GTT'AAC
Cuts at: 4176 4176
Size: 6586
KpnI G_GTAC'C
Cuts at: 2157 3911 2157
Size: 1754 4832
MfeI C'AATT_G
Cuts at: 4185 4185
Size: 6586
MluI A'CGCG_T
Cuts at: 6462 6462
Size: 6586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2364 2442 2502 3845 5700 5884 1305
Size: 1059 78 60 1343 1855 184 2007
Fragments arranged by size:
2007 1855 1343 1059 184 78 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2702 3743 10
Size: 54 1425 343 870 1041 2853
Fragments arranged by size:
2853 1425 1041 870 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2883 3377 4544 4903 5062 518
Size: 648 1717 494 1167 359 159 2042
Fragments arranged by size:
2042 1717 1167 648 494 359 159
MspA1I CmG'CkG
Cuts at: 1606 2971 2989 3019 3037 4002 4679 5827 6072
Size: 1365 18 30 18 965 677 1148 245
Cuts at: 6072 1606
Size: 2120
Fragments arranged by size:
2120 1365 1148 965 677 245 30 18
18
NaeI GCC'GGC
Cuts at: 2873 2873
Size: 6586
NcoI C'CATG_G
Cuts at: 513 1065 2706 513
Size: 552 1641 4393
Fragments arranged by size:
4393 1641 552
NdeI CA'TA_TG
Cuts at: 387 2446 2880 387
Size: 2059 434 4093
Fragments arranged by size:
4093 2059 434
NgoMIV G'CCGG_C
Cuts at: 2871 2871
Size: 6586
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6586
NotI GC'GGCC_GC
Cuts at: 4033 4033
Size: 6586
NruI TCG'CGA
Cuts at: 2202 2202
Size: 6586
NspI r_CATG'y
Cuts at: 1132 1229 2089 2100 2924 3521 3944 5489 1132
Size: 97 860 11 824 597 423 1545 2229
Fragments arranged by size:
2229 1545 860 824 597 423 97 11
PasI CC'CwG_GG
Cuts at: 1348 2108 2354 3319 3673 1348
Size: 760 246 965 354 4261
Fragments arranged by size:
4261 965 760 354 246
PciI A'CATG_T
Cuts at: 5485 5485
Size: 6586
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3455 1108
Size: 2347 4239
PmeI GTTT'AAAC
Cuts at: 3890 3890
Size: 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4655 4687 6168 6200 4655
Size: 32 1481 32 5041
Fragments arranged by size:
5041 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2722 2212
Size: 510 6076
PshAI GACnn'nnGTC
Cuts at: 1613 2430 1613
Size: 817 5769
PsiI TTA'TAA
Cuts at: 4156 4156
Size: 6586
PspOMI G'GGCC_C
Cuts at: 2120 2357 2527 2120
Size: 237 170 6179
Fragments arranged by size:
6179 237 170
PstI C_TGCA'G
Cuts at: 830 2232 2988 3091 3480 3487 3938 830
Size: 1402 756 103 389 7 451 3478
Fragments arranged by size:
3478 1402 756 451 389 103 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6586
PvuII CAG'CTG
Cuts at: 2971 2989 3037 2971
Size: 18 48 6520
Fragments arranged by size:
6520 48 18
RsrII CG'GwC_CG
Cuts at: 2035 2035
Size: 6586
SacI G_AGCT'C
Cuts at: 2961 2961
Size: 6586
SalI G'TCGA_C
Cuts at: 1936 3928 1936
Size: 1992 4594
SanDI GG'GwC_CC
Cuts at: 2722 2722
Size: 6586
SbfI CC_TGCA'GG
Cuts at: 3480 3938 3480
Size: 458 6128
ScaI AGT'ACT
Cuts at: 4834 6546 4834
Size: 1712 4874
SfcI C'TryA_G
Cuts at: 826 1045 2228 2984 3087 3476 3483 3934 5069
Size: 219 1183 756 103 389 7 451 1135
Cuts at: 5069 5750 5941 826
Size: 681 191 1471
Fragments arranged by size:
1471 1183 1135 756 681 451 389 219
191 103 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 3625 3625
Size: 6586
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6586
SmaI CCC'GGG
Cuts at: 1872 3913 1872
Size: 2041 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3044 3056 4690 5591 5853 820
Size: 197 926 1101 12 1634 901 262 1553
Fragments arranged by size:
1634 1553 1101 926 901 262 197 12
SnaBI TAC'GTA
Cuts at: 493 3883 4377 493
Size: 3390 494 2702
Fragments arranged by size:
3390 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6586
SphI G_CATG'C
Cuts at: 1132 2089 2100 3944 1132
Size: 957 11 1844 3774
Fragments arranged by size:
3774 1844 957 11
SspI AAT'ATT
Cuts at: 5 52 4510 5
Size: 47 4458 2081
Fragments arranged by size:
4458 2081 47
StuI AGG'CCT
Cuts at: 1517 3506 1517
Size: 1989 4597
StyI C'CwwG_G
Cuts at: 513 1065 2126 2706 3409 3775 4025 513
Size: 552 1061 580 703 366 250 3074
Fragments arranged by size:
3074 1061 703 580 552 366 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3586 3706 3742 4613 4630 4783 4968
Size: 1255 2006 120 36 871 17 153 185
Cuts at: 4968 5413 325
Size: 445 1498
Fragments arranged by size:
2006 1498 1255 871 445 185 153 120
36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2892 4832 6544
Size: 274 80 33 51 1443 915 1940 1712
Cuts at: 6544 96
Size: 138
Fragments arranged by size:
1940 1712 1443 915 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 3315 3347 6546 6578 3101
Size: 32 182 32 3199 32 3109
Fragments arranged by size:
3199 3109 182 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2566 1316
Size: 1250 5336
XbaI T'CTAG_A
Cuts at: 3922 3922
Size: 6586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2451 3736 2451
Size: 1285 5301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6586
XmaI C'CCGG_G
Cuts at: 1870 3911 1870
Size: 2041 4545
XmnI GAAnn'nnTTC
Cuts at: 4715 4715
Size: 6586
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4394 276
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI DraIII EcoNI
EcoRV FseI FspAI KasI NarI NsiI PacI PfoI
PmlI PspXI PsrI SacII SapI SexAI SfoI SrfI
SwaI