Restriction map for
pFN21ASDB0004
(Circular) (Six-base) MAPSORT of: pFN21ASDB0004.seq Check: 1890 from: 1 to: 7987
pFN21ASDB0004 7987 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4305 4563 4305
Size: 258 7729
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4674 5797 278
Size: 53 83 186 936 3138 1123 2468
Fragments arranged by size:
3138 2468 1123 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5330 1937
Size: 3393 4594
Acc65I G'GTAC_C
Cuts at: 5308 5308
Size: 7987
AclI AA'CG_TT
Cuts at: 1563 6114 6487 1563
Size: 4551 373 3063
Fragments arranged by size:
4551 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 3583 6050 7434 7702 1632
Size: 1069 882 2467 1384 268 1917
Fragments arranged by size:
2467 1917 1384 1069 882 268
AfeI AGC'GCT
Cuts at: 4473 4473
Size: 7987
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7790
AflIII A'CryG_T
Cuts at: 1203 6886 7863 1203
Size: 5683 977 1327
Fragments arranged by size:
5683 1327 977
AgeI A'CCGG_T
Cuts at: 2794 6818 2794
Size: 4024 3963
AhdI GACnn_n'nnGTC
Cuts at: 6716 6716
Size: 7987
AleI CACnn'nnGTG
Cuts at: 1166 2495 4299 1166
Size: 1329 1804 4854
Fragments arranged by size:
4854 1804 1329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7987
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 7955
AlwNI CAG_nnn'CTG
Cuts at: 2508 3195 3207 3343 3568 3664 5113 5205 5730
Size: 687 12 136 225 96 1449 92 525
Cuts at: 5730 7302 2508
Size: 1572 3193
Fragments arranged by size:
3193 1572 1449 687 525 225 136 96
92 12
ApaI G_GGCC'C
Cuts at: 2528 2681 3513 5271 2528
Size: 153 832 1758 5244
Fragments arranged by size:
5244 1758 832 153
ApaLI G'TGCA_C
Cuts at: 3067 4397 6044 7200 3067
Size: 1330 1647 1156 3854
Fragments arranged by size:
3854 1647 1330 1156
ApoI r'AATT_y
Cuts at: 1493 5296 5522 5674 7783 1493
Size: 3803 226 152 2109 1697
Fragments arranged by size:
3803 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6541 160
Size: 872 5509 1606
Fragments arranged by size:
5509 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7987
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 2854 2971 3251 3259 3506 3512
Size: 73 693 218 117 280 8 247 6
Cuts at: 3512 4783 4831 5312 1870
Size: 1271 48 481 4545
Fragments arranged by size:
4545 1271 693 481 280 247 218 117
73 48 8 6
BamHI G'GATC_C
Cuts at: 2550 5317 5684 2550
Size: 2767 367 4853
Fragments arranged by size:
4853 2767 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2645 2797 2939 3764 3980
Size: 325 217 627 858 152 142 825 216
Cuts at: 3980 4100 4208 4293 4742 5308 6763 618
Size: 120 108 85 449 566 1455 1842
Fragments arranged by size:
1842 1455 858 825 627 566 449 325
217 216 152 142 120 108 85
BanII G_rGCy'C
Cuts at: 1391 2313 2376 2451 2528 2681 2845 2855 3153
Size: 922 63 75 77 153 164 10 298
Cuts at: 3153 3513 4038 4311 4854 4977 5271 5306 1391
Size: 360 525 273 543 123 294 35 4072
Fragments arranged by size:
4072 922 543 525 360 298 294 273
164 153 123 77 75 63 35 10
BbeI G_GCGC'C
Cuts at: 3768 3984 3768
Size: 216 7771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3589 4330 928
Size: 971 1690 741 4585
Fragments arranged by size:
4585 1690 971 741
BbvCI CC'TCA_GC
Cuts at: 3336 4610 3336
Size: 1274 6713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6178 6212 1903
Size: 34 4241 34 3678
Fragments arranged by size:
4241 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5879 7089 5879
Size: 1210 6777
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7987
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3198 3233 3376
Size: 107 122 71 631 605 1526 35 143
Cuts at: 3376 4285 4757 4935 6598 136
Size: 909 472 178 1663 1525
Fragments arranged by size:
1663 1526 1525 909 631 605 472 178
143 122 107 71 35
BglII A'GATC_T
Cuts at: 7982 7982
Size: 7987
BlpI GC'TnA_GC
Cuts at: 2144 4010 5404 2144
Size: 1866 1394 4727
Fragments arranged by size:
4727 1866 1394
Bme1580I G_kGCm'C
Cuts at: 1790 2528 2681 3071 3513 4211 4296 4401 4745
Size: 738 153 390 442 698 85 105 344
Cuts at: 4745 5271 6048 7204 1790
Size: 526 777 1156 2573
Fragments arranged by size:
2573 1156 777 738 698 526 442 390
344 153 105 85
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4732 5718 6676
Size: 416 53 83 399 24 3315 986 958
Cuts at: 6676 442
Size: 1753
Fragments arranged by size:
3315 1753 986 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7987
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4510 4510
Size: 7987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2936 3016 3923 4505 4520 4700 4747 6647
Size: 1697 80 907 582 15 180 47 1900
Cuts at: 6647 1239
Size: 2579
Fragments arranged by size:
2579 1900 1697 907 582 180 80 47
15
Bpu10I CC'TnA_GC
Cuts at: 3336 3686 4610 3336
Size: 350 924 6713
Fragments arranged by size:
6713 924 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6112 6977 7275 6112
Size: 865 298 6824
Fragments arranged by size:
6824 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6650 882
Size: 617 5151 2219
Fragments arranged by size:
5151 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3397 3618 3798 4813 5020 5284 5778
Size: 711 2193 221 180 1015 207 264 494
Cuts at: 5778 493
Size: 2702
Fragments arranged by size:
2702 2193 1015 711 494 264 221 207
180
BsaBI GATnn'nnATC
Cuts at: 7981 7981
Size: 7987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3765 3981 4335 4539
Size: 53 83 186 936 2232 216 354 204
Cuts at: 4539 4671 4884 5794 6176 6811 275
Size: 132 213 910 382 635 1451
Fragments arranged by size:
2232 1451 936 910 635 382 354 216
213 204 186 132 83 53
BsaWI w'CCGG_w
Cuts at: 2794 6420 6818 7092 7239 7650 2794
Size: 3626 398 274 147 411 3131
Fragments arranged by size:
3626 3131 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2616 2646 2991 3021 4073
Size: 30 165 30 1213 30 345 30 1052
Cuts at: 4073 4103 4361 4391 1178
Size: 30 258 30 4774
Fragments arranged by size:
4774 1213 1052 345 258 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2420 2423 2432 2441 2444 2447 3842
Size: 982 252 3 9 9 3 3 1395
Cuts at: 3842 4046 1186
Size: 204 5127
Fragments arranged by size:
5127 1395 982 252 204 9 9 3
3 3
BseYI C'CCAG_C
Cuts at: 2460 2662 2680 2781 2811 2859 2864 3188 3351
Size: 202 18 101 30 48 5 324 163
Cuts at: 3351 3667 4085 4439 4863 5127 7190 7686 2460
Size: 316 418 354 424 264 2063 496 2761
Fragments arranged by size:
2761 2063 496 424 418 354 324 316
264 202 163 101 48 30 18 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2041 2164 2468 3110 3566 3777 3902
Size: 573 305 123 304 642 456 211 125
Cuts at: 3902 4445 5000 5017 5042 5084 1163
Size: 543 555 17 25 42 4066
Fragments arranged by size:
4066 642 573 555 543 456 305 304
211 125 123 42 25 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2076 2595 3716 5276 5437
Size: 275 186 33 42 519 1121 1560 161
Cuts at: 5437 6198 7226 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1560 1121 1028 761 519 275 186
161 42 33
BsiHKAI G_wGCw'C
Cuts at: 2093 3071 3104 4311 4401 6048 6133 7204 2093
Size: 978 33 1207 90 1647 85 1071 2876
Fragments arranged by size:
2876 1647 1207 1071 978 90 85 33
BsmI GAATG_Cn'
Cuts at: 5498 5591 5498
Size: 93 7894
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2313 2376 2451 2528 2681 2845
Size: 399 303 220 63 75 77 153 164
Cuts at: 2845 2855 3071 3104 3153 3513 4038 4211 4296
Size: 10 216 33 49 360 525 173 85
Cuts at: 4296 4311 4401 4745 4854 4977 5271 5306 6048
Size: 15 90 344 109 123 294 35 742
Cuts at: 6048 6133 7204 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 525 399 360 344 303
294 220 216 173 164 153 123 109
90 85 85 77 75 63 49 35
33 15 10
BspEI T'CCGG_A
Cuts at: 7650 7650
Size: 7987
BspHI T'CATG_A
Cuts at: 5876 7606 5876
Size: 1730 6257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2327 4305 4563 4952 5022 5098 5342 844
Size: 1483 1978 258 389 70 76 244 3489
Fragments arranged by size:
3489 1978 1483 389 258 244 76 70
BsrBI CCG'CTC
Cuts at: 1394 1823 2441 3712 3730 4489 4918 5239 5874
Size: 429 618 1271 18 759 429 321 635
Cuts at: 5874 1394
Size: 3507
Fragments arranged by size:
3507 1271 759 635 618 429 429 321
18
BsrDI GCAATG_nn'
Cuts at: 65 1248 6482 6656 65
Size: 1183 5234 174 1396
Fragments arranged by size:
5234 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 2594 2794 2828 2957 3842 3977
Size: 110 265 519 200 34 129 885 135
Cuts at: 3977 5144 5255 6631 6818 1700
Size: 1167 111 1376 187 2869
Fragments arranged by size:
2869 1376 1167 885 519 265 200 187
135 129 111 110 34
BsrGI T'GTAC_A
Cuts at: 96 4824 96
Size: 4728 3259
BssHII G'CGCG_C
Cuts at: 1925 4452 4642 1925
Size: 2527 190 5270
Fragments arranged by size:
5270 2527 190
BssSI C'ACGA_G
Cuts at: 4922 6047 7059 4922
Size: 1125 1012 5850
Fragments arranged by size:
5850 1125 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3207 3568 3664 4944 794
Size: 2413 361 96 1280 3837
Fragments arranged by size:
3837 2413 1280 361 96
BstBI TT'CG_AA
Cuts at: 2007 6791 2007
Size: 4784 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7987
BstXI CCAn_nnnn'nTGG
Cuts at: 3143 3811 3143
Size: 668 7319
BstYI r'GATC_y
Cuts at: 1972 2550 5317 5684 6069 6086 7538 7624 7636
Size: 578 2767 367 385 17 1452 86 12
Cuts at: 7636 7982 1972
Size: 346 1977
Fragments arranged by size:
2767 1977 1452 578 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2559 2802 3374 2559
Size: 243 572 7172
Fragments arranged by size:
7172 572 243
BtgI C'CryG_G
Cuts at: 513 1065 2022 2028 2367 2496 2546 2928 3531
Size: 552 957 6 339 129 50 382 603
Cuts at: 3531 3804 4235 4380 4458 4542 4854 4909 513
Size: 273 431 145 78 84 312 55 3591
Fragments arranged by size:
3591 957 603 552 431 382 339 312
273 145 129 84 78 55 50 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5159 487
Size: 683 56 3933 3315
Fragments arranged by size:
3933 3315 683 56
BtsI GCAGTG_nn'
Cuts at: 2393 2983 3160 3299 3313 3569 3635 4333 5030
Size: 590 177 139 14 256 66 698 697
Cuts at: 5030 5503 6297 6317 2393
Size: 473 794 20 4063
Fragments arranged by size:
4063 794 698 697 590 473 256 177
139 66 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7987
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7952
DraI TTT'AAA
Cuts at: 5291 5291
Size: 7987
DraIII CAC_nnn'GTG
Cuts at: 2608 2608
Size: 7987
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6994 809
Size: 507 5678 1802
Fragments arranged by size:
5678 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2073 2077
Size: 54 1425 50 275 18 195 48 4
Cuts at: 2077 2364 2499 2592 2994 3713 3909 3966 4277
Size: 287 135 93 402 719 196 57 311
Cuts at: 4277 4286 4446 4603 4878 5434 6323 7952 8
Size: 9 160 157 275 556 889 1629 43
Fragments arranged by size:
1629 1425 889 719 556 402 311 287
275 275 196 195 160 157 135 93
57 54 50 48 43 18 9 4
EagI C'GGCC_G
Cuts at: 1537 1812 2073 2592 3713 5434 1537
Size: 275 261 519 1121 1721 4090
Fragments arranged by size:
4090 1721 1121 519 275 261
EarI CTCTTCn'nnn_
Cuts at: 1353 2916 3860 4517 5917 1353
Size: 1563 944 657 1400 3423
Fragments arranged by size:
3423 1563 1400 944 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2459 3722 6574 6948 7094 387
Size: 1064 138 870 1263 2852 374 146 1280
Fragments arranged by size:
2852 1280 1263 1064 870 374 146 138
EcoICRI GAG'CTC
Cuts at: 4309 4309
Size: 7987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2701 2936 3016 3583 3923 4505 4520
Size: 393 1069 235 80 567 340 582 15
Cuts at: 4520 4700 4747 6050 6647 7434 7702 1239
Size: 180 47 1303 597 787 268 1524
Fragments arranged by size:
1524 1303 1069 787 597 582 567 393
340 268 235 180 80 47 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2201
Size: 7987
EcoO109I rG'GnC_Cy
Cuts at: 2278 2524 2563 2677 2707 2868 4780 5110 5223
Size: 246 39 114 30 161 1912 330 113
Cuts at: 5223 5268 2278
Size: 45 4997
Fragments arranged by size:
4997 1912 330 246 161 114 113 45
39 30
EcoRI G'AATT_C
Cuts at: 5296 5674 5296
Size: 378 7609
FseI GG_CCGG'CC
Cuts at: 2079 2079
Size: 7987
FspI TGC'GCA
Cuts at: 6493 6493
Size: 7987
HaeII r_GCGC'y
Cuts at: 1678 2086 3259 3693 3768 3908 3984 4004 4475
Size: 408 1173 434 75 140 76 20 471
Cuts at: 4475 4534 7134 1678
Size: 59 2600 2531
Fragments arranged by size:
2600 2531 1173 471 434 408 140 76
75 59 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2876 2908 6628 6660 6702
Size: 32 135 32 1304 32 3720 32 42
Cuts at: 6702 6734 1373
Size: 32 2626
Fragments arranged by size:
3720 2626 1304 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4042 5331 5577 669
Size: 988 281 2104 1289 246 3079
Fragments arranged by size:
3079 2104 1289 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7987
HpaI GTT'AAC
Cuts at: 5577 5577
Size: 7987
KasI G'GCGC_C
Cuts at: 3764 3980 3764
Size: 216 7771
KpnI G_GTAC'C
Cuts at: 5312 5312
Size: 7987
MfeI C'AATT_G
Cuts at: 5586 5586
Size: 7987
MluI A'CGCG_T
Cuts at: 7863 7863
Size: 7987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2014 4293 7101 7285 1305
Size: 709 2279 2808 184 2007
Fragments arranged by size:
2808 2279 2007 709 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2366 2501 3911 3968
Size: 54 1425 343 195 339 135 1410 57
Cuts at: 3968 4279 10
Size: 311 3718
Fragments arranged by size:
3718 1425 1410 343 339 311 195 135
57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3395 4127 4299 4574 4982 5945
Size: 648 1329 900 732 172 275 408 963
Cuts at: 5945 6304 6463 518
Size: 359 159 2042
Fragments arranged by size:
2042 1329 963 900 732 648 408 359
275 172 159
MspA1I CmG'CkG
Cuts at: 1606 2178 2322 2684 2785 3355 3406 3533 3871
Size: 572 144 362 101 570 51 127 338
Cuts at: 3871 3880 4263 4599 4665 4911 5278 5403 6080
Size: 9 383 336 66 246 367 125 677
Cuts at: 6080 7228 7473 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 572 570 383 367 362
338 336 246 245 144 127 125 101
66 51 9
NaeI GCC'GGC
Cuts at: 2077 2596 2830 2959 3844 5146 2077
Size: 519 234 129 885 1302 4918
Fragments arranged by size:
4918 1302 885 519 234 129
NarI GG'CG_CC
Cuts at: 3765 3981 3765
Size: 216 7771
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6478
Fragments arranged by size:
6478 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7987
NgoMIV G'CCGG_C
Cuts at: 2075 2594 2828 2957 3842 5144 2075
Size: 519 234 129 885 1302 4918
Fragments arranged by size:
4918 1302 885 519 234 129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7987
NotI GC'GGCC_GC
Cuts at: 5434 5434
Size: 7987
NspI r_CATG'y
Cuts at: 1132 1229 3394 3826 4175 5345 6890 1132
Size: 97 2165 432 349 1170 1545 2229
Fragments arranged by size:
2229 2165 1545 1170 432 349 97
PasI CC'CwG_GG
Cuts at: 1348 2703 2848 1348
Size: 1355 145 6487
Fragments arranged by size:
6487 1355 145
PciI A'CATG_T
Cuts at: 6886 6886
Size: 7987
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4558 4816 5167 1108
Size: 3450 258 351 3928
Fragments arranged by size:
3928 3450 351 258
PfoI T'CCnGG_A
Cuts at: 2574 3135 4185 4250 4515 2574
Size: 561 1050 65 265 6046
Fragments arranged by size:
6046 1050 561 265 65
PmeI GTTT'AAAC
Cuts at: 5291 5291
Size: 7987
PmlI CAC'GTG
Cuts at: 3397 3618 3798 4813 5020 3397
Size: 221 180 1015 207 6364
Fragments arranged by size:
6364 1015 221 207 180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 6056 6088 7569 7601 4073
Size: 32 1951 32 1481 32 4459
Fragments arranged by size:
4459 1951 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2707 4780 5110 5223 2278
Size: 429 2073 330 113 5042
Fragments arranged by size:
5042 2073 429 330 113
PshAI GACnn'nnGTC
Cuts at: 1613 3217 1613
Size: 1604 6383
PsiI TTA'TAA
Cuts at: 5557 5557
Size: 7987
PspOMI G'GGCC_C
Cuts at: 2524 2677 3509 5267 2524
Size: 153 832 1758 5244
Fragments arranged by size:
5244 1758 832 153
PspXI vC'TCGA_Gb
Cuts at: 4831 4831
Size: 7987
PstI C_TGCA'G
Cuts at: 830 2742 2790 2837 3660 4008 5339 830
Size: 1912 48 47 823 348 1331 3478
Fragments arranged by size:
3478 1912 1331 823 348 48 47
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7987
PvuII CAG'CTG
Cuts at: 2322 2684 2785 3880 4263 4665 2322
Size: 362 101 1095 383 402 5644
Fragments arranged by size:
5644 1095 402 383 362 101
SacI G_AGCT'C
Cuts at: 4311 4311
Size: 7987
SacII CC_GC'GG
Cuts at: 3534 4912 3534
Size: 1378 6609
SalI G'TCGA_C
Cuts at: 1936 5329 1936
Size: 3393 4594
SanDI GG'GwC_CC
Cuts at: 2707 4780 5223 2707
Size: 2073 443 5471
Fragments arranged by size:
5471 2073 443
SapI GCTCTTCn'nnn_
Cuts at: 4517 4517
Size: 7987
SbfI CC_TGCA'GG
Cuts at: 3660 5339 3660
Size: 1679 6308
ScaI AGT'ACT
Cuts at: 6235 7947 6235
Size: 1712 6275
SexAI A'CCwGG_T
Cuts at: 3050 3720 3771 3050
Size: 670 51 7266
Fragments arranged by size:
7266 670 51
SfcI C'TryA_G
Cuts at: 826 1045 2738 2786 2833 3656 4004 5335 6470
Size: 219 1693 48 47 823 348 1331 1135
Cuts at: 6470 7151 7342 826
Size: 681 191 1471
Fragments arranged by size:
1693 1471 1331 1135 823 681 348 219
191 48 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 3233 4285 3233
Size: 1052 6935
SfoI GGC'GCC
Cuts at: 3766 3982 3766
Size: 216 7771
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7987
SgrAI Cr'CCGG_yG
Cuts at: 1700 2794 3977 1700
Size: 1094 1183 5710
Fragments arranged by size:
5710 1183 1094
SmaI CCC'GGG
Cuts at: 1872 2638 3261 3508 5314 1872
Size: 766 623 247 1806 4545
Fragments arranged by size:
4545 1806 766 623 247
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4831 6091 6992 7254 820
Size: 197 926 2888 1260 901 262 1553
Fragments arranged by size:
2888 1553 1260 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5284 5778 493
Size: 4791 494 2702
Fragments arranged by size:
4791 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7987
SphI G_CATG'C
Cuts at: 1132 3394 4175 5345 1132
Size: 2262 781 1170 3774
Fragments arranged by size:
3774 2262 1170 781
SrfI GCCC'GGGC
Cuts at: 3261 3261
Size: 7987
SspI AAT'ATT
Cuts at: 5 52 5911 5
Size: 47 5859 2081
Fragments arranged by size:
5859 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 3963 4760 5009 5192 1517
Size: 693 1753 797 249 183 4312
Fragments arranged by size:
4312 1753 797 693 249 183
StyI C'CwwG_G
Cuts at: 513 1065 2022 2313 3432 5004 5217 5426 513
Size: 552 957 291 1119 1572 213 209 3074
Fragments arranged by size:
3074 1572 1119 957 552 291 213 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3173 3874 4088 5174 6014 6031 6184
Size: 1255 1593 701 214 1086 840 17 153
Cuts at: 6184 6369 6814 325
Size: 185 445 1498
Fragments arranged by size:
1593 1498 1255 1086 840 701 445 214
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4824 6233 7945
Size: 274 80 33 51 1443 2847 1409 1712
Cuts at: 7945 96
Size: 138
Fragments arranged by size:
2847 1712 1443 1409 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 2616 2648 2991 3023 3782 3814 7947
Size: 32 301 32 343 32 759 32 4133
Cuts at: 7947 7979 2283
Size: 32 2291
Fragments arranged by size:
4133 2291 759 343 301 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4361 1316
Size: 3045 4942
XbaI T'CTAG_A
Cuts at: 5323 5323
Size: 7987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3811 3961 4456 3811
Size: 150 495 7342
Fragments arranged by size:
7342 495 150
XhoI C'TCGA_G
Cuts at: 1943 4831 1943
Size: 2888 5099
XmaI C'CCGG_G
Cuts at: 1870 2636 3259 3506 5312 1870
Size: 766 623 247 1806 4545
Fragments arranged by size:
4545 1806 766 623 247
XmnI GAAnn'nnTTC
Cuts at: 6116 6116
Size: 7987
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4672 5795 276
Size: 53 83 186 936 3138 1123 2468
Fragments arranged by size:
3138 2468 1123 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PsrI RsrII SwaI