Restriction map for
pFN21ASDA2013
(Circular) (Six-base) MAPSORT of: pFN21ASDA2013.seq Check: 5727 from: 1 to: 6730
pFN21ASDA2013 6730 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2175 3720 2175
Size: 1545 5185
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4540 278
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 3197 4073 1937
Size: 688 572 876 4594
Fragments arranged by size:
4594 876 688 572
Acc65I G'GTAC_C
Cuts at: 2269 4051 2269
Size: 1782 4948
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2448 3031 3208 3883 4793 6177 6445 1632
Size: 816 583 177 675 910 1384 268 1917
Fragments arranged by size:
1917 1384 910 816 675 583 268 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6533
AflIII A'CryG_T
Cuts at: 1203 3544 3546 5629 6606 1203
Size: 2341 2 2083 977 1327
Fragments arranged by size:
2341 2083 1327 977 2
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 2621 5459 2621
Size: 2838 3892
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6730
AlwNI CAG_nnn'CTG
Cuts at: 2507 3292 3721 4473 6045 2507
Size: 785 429 752 1572 3192
Fragments arranged by size:
3192 1572 785 752 429
ApaI G_GGCC'C
Cuts at: 2080 2535 2691 2080
Size: 455 156 6119
Fragments arranged by size:
6119 455 156
ApaLI G'TGCA_C
Cuts at: 2798 2888 4787 5943 2798
Size: 90 1899 1156 3585
Fragments arranged by size:
3585 1899 1156 90
ApoI r'AATT_y
Cuts at: 1493 4039 4265 4417 6526 1493
Size: 2546 226 152 2109 1697
Fragments arranged by size:
2546 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5284 160
Size: 872 4252 1606
Fragments arranged by size:
4252 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 2354 2528 3785 3936 4055 1870
Size: 73 411 174 1257 151 119 4545
Fragments arranged by size:
4545 1257 411 174 151 119 73
BamHI G'GATC_C
Cuts at: 4060 4427 4060
Size: 367 6363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 2269 2377 3359 3631
Size: 325 217 627 408 74 108 982 272
Cuts at: 3631 4051 5506 618
Size: 420 1455 1842
Fragments arranged by size:
1842 1455 982 627 420 408 325 272
217 108 74
BanII G_rGCy'C
Cuts at: 1391 2080 2370 2535 2691 2946 2988 3040 4049
Size: 689 290 165 156 255 42 52 1009
Cuts at: 4049 1391
Size: 4072
Fragments arranged by size:
4072 1009 689 290 255 165 156 52
42
BbeI G_GCGC'C
Cuts at: 2199 2381 3635 2199
Size: 182 1254 5294
Fragments arranged by size:
5294 1254 182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2192 2226 4921 4955 1903
Size: 34 255 34 2695 34 3678
Fragments arranged by size:
3678 2695 255 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4622 5832 4622
Size: 1210 5520
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6730
BfrBI ATG'CAT
Cuts at: 3467 3467
Size: 6730
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 2300 2711
Size: 107 122 71 631 605 428 200 411
Cuts at: 2711 3268 3515 3593 3752 5341 136
Size: 557 247 78 159 1589 1525
Fragments arranged by size:
1589 1525 631 605 557 428 411 247
200 159 122 107 78 71
BglII A'GATC_T
Cuts at: 3579 6725 3579
Size: 3146 3584
BlpI GC'TnA_GC
Cuts at: 2414 3039 3155 3389 4147 2414
Size: 625 116 234 758 4997
Fragments arranged by size:
4997 758 625 234 116
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2394 2535 2691 2802 2892 3234 3364
Size: 290 314 141 156 111 90 342 130
Cuts at: 3364 4791 5947 1790
Size: 1427 1156 2573
Fragments arranged by size:
2573 1427 1156 342 314 290 156 141
130 111 90
BmgBI CAC'GTC
Cuts at: 1318 2326 2623 2893 3448 1318
Size: 1008 297 270 555 4600
Fragments arranged by size:
4600 1008 555 297 270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2799 4461 5419
Size: 416 53 83 399 24 1382 1662 958
Cuts at: 5419 442
Size: 1753
Fragments arranged by size:
1753 1662 1382 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3983 1056
Size: 2927 3803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 6730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2304 2454 2750 3002 3308 3410 3903
Size: 1001 64 150 296 252 306 102 493
Cuts at: 3903 5390 1239
Size: 1487 2579
Fragments arranged by size:
2579 1487 1001 493 306 296 252 150
102 64
Bpu10I CC'TnA_GC
Cuts at: 3167 3167
Size: 6730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2930 3725 4855 5720 6018 2027
Size: 903 795 1130 865 298 2739
Fragments arranged by size:
2739 1130 903 865 795 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2736 3177 5393 882
Size: 617 1237 441 2216 2219
Fragments arranged by size:
2219 2216 1237 617 441
BsaAI yAC'GTr
Cuts at: 493 1204 3547 4027 4521 493
Size: 711 2343 480 494 2702
Fragments arranged by size:
2702 2343 711 494 480
BsaBI GATnn'nnATC
Cuts at: 6724 6724
Size: 6730
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 2378 3632 4537
Size: 53 83 186 936 663 182 1254 905
Cuts at: 4537 4919 5554 275
Size: 382 635 1451
Fragments arranged by size:
1451 1254 936 905 663 635 382 186
182 83 53
BsaWI w'CCGG_w
Cuts at: 5163 5561 5835 5982 6393 5163
Size: 398 274 147 411 5500
Fragments arranged by size:
5500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2427 2457 3116 3146 1178
Size: 30 165 30 1024 30 659 30 4762
Fragments arranged by size:
4762 1024 659 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2747 3020 1186
Size: 892 669 273 4896
Fragments arranged by size:
4896 892 669 273
BseYI C'CCAG_C
Cuts at: 2073 2275 2491 2701 3774 5933 6429 2073
Size: 202 216 210 1073 2159 496 2374
Fragments arranged by size:
2374 2159 1073 496 216 210 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2420 3437 3719 1163
Size: 573 411 273 1017 282 4174
Fragments arranged by size:
4174 1017 573 411 282 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 4180 4941 5969 1540
Size: 275 186 568 1611 761 1028 2301
Fragments arranged by size:
2301 1611 1028 761 568 275 186
BsiHKAI G_wGCw'C
Cuts at: 2802 2892 2922 2946 2988 3390 3768 4791 4876
Size: 90 30 24 42 402 378 1023 85
Cuts at: 4876 5947 2802
Size: 1071 3585
Fragments arranged by size:
3585 1071 1023 402 378 90 85 42
30 24
BsmI GAATG_Cn'
Cuts at: 4241 4334 4241
Size: 93 6637
BsmBI CGTCTCn'nnnn_
Cuts at: 2548 2864 2899 2548
Size: 316 35 6379
Fragments arranged by size:
6379 316 35
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2370 2394 2535 2691 2802 2892
Size: 399 290 290 24 141 156 111 90
Cuts at: 2892 2922 2946 2988 3040 3234 3364 3390 3768
Size: 30 24 42 52 194 130 26 378
Cuts at: 3768 4049 4791 4876 5947 1391
Size: 281 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 290 290 281
194 156 141 130 111 90 85 52
42 30 26 24 24
BspEI T'CCGG_A
Cuts at: 6393 6393
Size: 6730
BspHI T'CATG_A
Cuts at: 4619 6349 4619
Size: 1730 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2637 3720 3729 4085 844
Size: 1331 462 1083 9 356 3489
Fragments arranged by size:
3489 1331 1083 462 356 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 4617 1394
Size: 429 539 2255 3507
Fragments arranged by size:
3507 2255 539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3467 5225 5399 65
Size: 1183 2219 1758 174 1396
Fragments arranged by size:
2219 1758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2290 2522 2696 2769 5374 5561 1700
Size: 110 480 232 174 73 2605 187 2869
Fragments arranged by size:
2869 2605 480 232 187 174 110 73
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6730
BssHII G'CGCG_C
Cuts at: 1925 2139 2418 1925
Size: 214 279 6237
Fragments arranged by size:
6237 279 214
BssSI C'ACGA_G
Cuts at: 4790 5802 4790
Size: 1012 5718
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 3292 3721 3733 794
Size: 1376 1122 429 12 3791
Fragments arranged by size:
3791 1376 1122 429 12
BstBI TT'CG_AA
Cuts at: 2007 5534 2007
Size: 3527 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6730
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2794 2827 3332 2728
Size: 66 33 505 6126
Fragments arranged by size:
6126 505 66 33
BstYI r'GATC_y
Cuts at: 1972 3288 3579 4060 4427 4812 4829 6281 6367
Size: 1316 291 481 367 385 17 1452 86
Cuts at: 6367 6379 6725 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1316 481 385 367 346 291
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2182 2373 2514 2556 2655 3552 3562
Size: 552 1117 191 141 42 99 897 10
Cuts at: 3562 3636 513
Size: 74 3607
Fragments arranged by size:
3607 1117 897 552 191 141 99 74
42 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5991
Fragments arranged by size:
5991 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 3149 3217 3680 4246 5040 5060 2691
Size: 458 68 463 566 794 20 4361
Fragments arranged by size:
4361 794 566 463 458 68 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6730
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6695
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DraIII CAC_nnn'GTG
Cuts at: 2806 2806
Size: 6730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5737 809
Size: 507 4421 1802
Fragments arranged by size:
4421 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2301 2517
Size: 54 1425 50 275 18 396 75 216
Cuts at: 2517 2566 2667 2814 3260 3507 3565 4177 5066
Size: 49 101 147 446 247 58 612 889
Cuts at: 5066 6695 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 612 446 396 275 247
216 147 101 75 58 54 50 49
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2566 4177 1537
Size: 275 754 1611 4090
Fragments arranged by size:
4090 1611 754 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3050 3806 3839 3848 3854 3902 4660 1353
Size: 1697 756 33 9 6 48 758 3423
Fragments arranged by size:
3423 1697 758 756 48 33 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3020 3533 5317 5691 5837 387
Size: 1064 138 1431 513 1784 374 146 1280
Fragments arranged by size:
1784 1431 1280 1064 513 374 146 138
EcoICRI GAG'CTC
Cuts at: 2944 2986 2944
Size: 42 6688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2304 2448 2454 2750 3002 3031
Size: 393 608 64 144 6 296 252 29
Cuts at: 3031 3208 3308 3410 3883 3903 4793 5390 6177
Size: 177 100 102 473 20 890 597 787
Cuts at: 6177 6445 1239
Size: 268 1524
Fragments arranged by size:
1524 890 787 608 597 473 393 296
268 252 177 144 102 100 64 29
20 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2732 2732
Size: 6730
EcoO109I rG'GnC_Cy
Cuts at: 2279 2496 2504 2687 3718 3781 2279
Size: 217 8 183 1031 63 5228
Fragments arranged by size:
5228 1031 217 183 63 8
EcoRI G'AATT_C
Cuts at: 4039 4417 4039
Size: 378 6352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3913 3913
Size: 6730
FspI TGC'GCA
Cuts at: 5236 5236
Size: 6730
HaeII r_GCGC'y
Cuts at: 1678 2199 2381 2954 3635 5877 1678
Size: 521 182 573 681 2242 2531
Fragments arranged by size:
2531 2242 681 573 521 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2607 2639 5371 5403 5445
Size: 32 135 32 1035 32 2732 32 42
Cuts at: 5445 5477 1373
Size: 32 2626
Fragments arranged by size:
2732 2626 1035 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3198 4074 4320 669
Size: 988 281 1260 876 246 3079
Fragments arranged by size:
3079 1260 988 876 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6730
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KasI G'GCGC_C
Cuts at: 2195 2377 3631 2195
Size: 182 1254 5294
Fragments arranged by size:
5294 1254 182
KpnI G_GTAC'C
Cuts at: 2273 4055 2273
Size: 1782 4948
MfeI C'AATT_G
Cuts at: 4329 4329
Size: 6730
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2719 5844 6028 1305
Size: 1414 3125 184 2007
Fragments arranged by size:
3125 2007 1414 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 3567 10
Size: 54 1425 343 471 1264 3173
Fragments arranged by size:
3173 1425 1264 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4688 5047 5206 518
Size: 648 3522 359 159 2042
Fragments arranged by size:
3522 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2073 2184 2275 2404 2413 2491 2558
Size: 432 35 111 91 129 9 78 67
Cuts at: 2558 2863 3002 3228 4146 4823 5971 6216 1606
Size: 305 139 226 918 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 432 305 245 226
139 129 111 91 78 67 35 9
NaeI GCC'GGC
Cuts at: 2292 2524 2698 2292
Size: 232 174 6324
Fragments arranged by size:
6324 232 174
NarI GG'CG_CC
Cuts at: 2196 2378 3632 2196
Size: 182 1254 5294
Fragments arranged by size:
5294 1254 182
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6178
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6730
NgoMIV G'CCGG_C
Cuts at: 2290 2522 2696 2290
Size: 232 174 6324
Fragments arranged by size:
6324 232 174
NheI G'CTAG_C
Cuts at: 1052 3979 1052
Size: 2927 3803
NotI GC'GGCC_GC
Cuts at: 4177 4177
Size: 6730
NsiI A_TGCA'T
Cuts at: 3469 3469
Size: 6730
NspI r_CATG'y
Cuts at: 1132 1229 2998 3130 4088 5633 1132
Size: 97 1769 132 958 1545 2229
Fragments arranged by size:
2229 1769 1545 958 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6730
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2507 1108
Size: 1399 5331
PfoI T'CCnGG_A
Cuts at: 2281 2281
Size: 6730
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PmlI CAC'GTG
Cuts at: 3547 3547
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4799 4831 6312 6344 4799
Size: 32 1481 32 5185
Fragments arranged by size:
5185 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2279 2496 2504 3718 3781 2279
Size: 217 8 1214 63 5228
Fragments arranged by size:
5228 1214 217 63 8
PshAI GACnn'nnGTC
Cuts at: 1613 2062 1613
Size: 449 6281
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PspOMI G'GGCC_C
Cuts at: 2076 2531 2687 2076
Size: 455 156 6119
Fragments arranged by size:
6119 455 156
PstI C_TGCA'G
Cuts at: 830 2034 2490 2634 2679 2958 3297 3378 3408
Size: 1204 456 144 45 279 339 81 30
Cuts at: 3408 3717 4082 830
Size: 309 365 3478
Fragments arranged by size:
3478 1204 456 365 339 309 279 144
81 45 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 2073 2404 2491 2863 2073
Size: 331 87 372 5940
Fragments arranged by size:
5940 372 331 87
SacI G_AGCT'C
Cuts at: 2946 2988 2946
Size: 42 6688
SacII CC_GC'GG
Cuts at: 2185 2559 2185
Size: 374 6356
SalI G'TCGA_C
Cuts at: 1936 3196 4072 1936
Size: 1260 876 4594
Fragments arranged by size:
4594 1260 876
SanDI GG'GwC_CC
Cuts at: 2496 2496
Size: 6730
SapI GCTCTTCn'nnn_
Cuts at: 3050 3050
Size: 6730
SbfI CC_TGCA'GG
Cuts at: 3378 3717 4082 3378
Size: 339 365 6026
Fragments arranged by size:
6026 365 339
ScaI AGT'ACT
Cuts at: 3872 4978 6690 3872
Size: 1106 1712 3912
Fragments arranged by size:
3912 1712 1106
SexAI A'CCwGG_T
Cuts at: 2802 2802
Size: 6730
SfcI C'TryA_G
Cuts at: 826 1045 2030 2486 2630 2675 2954 3293 3374
Size: 219 985 456 144 45 279 339 81
Cuts at: 3374 3404 3713 4078 5213 5894 6085 826
Size: 30 309 365 1135 681 191 1471
Fragments arranged by size:
1471 1135 985 681 456 365 339 309
279 219 191 144 81 45 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2300 3752 2300
Size: 1452 5278
SfoI GGC'GCC
Cuts at: 2197 2379 3633 2197
Size: 182 1254 5294
Fragments arranged by size:
5294 1254 182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 2356 2530 3787 4057 1872
Size: 484 174 1257 270 4545
Fragments arranged by size:
4545 1257 484 270 174
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2945 3740 4834 5735 5997
Size: 197 926 99 903 795 1094 901 262
Cuts at: 5997 820
Size: 1553
Fragments arranged by size:
1553 1094 926 903 901 795 262 197
99
SnaBI TAC'GTA
Cuts at: 493 4027 4521 493
Size: 3534 494 2702
Fragments arranged by size:
3534 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 4088 1132
Size: 2956 3774
SspI AAT'ATT
Cuts at: 5 52 4654 5
Size: 47 4602 2081
Fragments arranged by size:
4602 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 3942 4003 1517
Size: 1197 1228 61 4244
Fragments arranged by size:
4244 1228 1197 61
StyI C'CwwG_G
Cuts at: 513 1065 2709 2937 3943 4169 513
Size: 552 1644 228 1006 226 3074
Fragments arranged by size:
3074 1644 1006 552 228 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2335 2872 3098 3347 3643 4006 4757
Size: 1255 755 537 226 249 296 363 751
Cuts at: 4757 4774 4927 5112 5557 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 755 751 537 445 363 296
249 226 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3870 4976 6688
Size: 274 80 33 51 1443 1893 1106 1712
Cuts at: 6688 96
Size: 138
Fragments arranged by size:
1893 1712 1443 1106 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2427 2459 6690 6722 2427
Size: 32 4231 32 2435
Fragments arranged by size:
4231 2435 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6730
XbaI T'CTAG_A
Cuts at: 4066 4066
Size: 6730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2662 2860 3092 2311
Size: 351 198 232 5949
Fragments arranged by size:
5949 351 232 198
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6730
XmaI C'CCGG_G
Cuts at: 1870 2354 2528 3785 4055 1870
Size: 484 174 1257 270 4545
Fragments arranged by size:
4545 1257 484 270 174
XmnI GAAnn'nnTTC
Cuts at: 4859 4859
Size: 6730
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4538 276
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NruI PacI PspXI PsrI
RsrII SrfI SwaI