Restriction map for
pFN21ASDA2008
(Circular) (Six-base) MAPSORT of: pFN21ASDA2008.seq Check: 8979 from: 1 to: 7105
pFN21ASDA2008 7105 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3908 4139 4198 3908
Size: 231 59 6815
Fragments arranged by size:
6815 231 59
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2141 4915 278
Size: 53 83 186 936 605 2774 2468
Fragments arranged by size:
2774 2468 936 605 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3813 3873 4448 1937
Size: 1876 60 575 4594
Fragments arranged by size:
4594 1876 575 60
Acc65I G'GTAC_C
Cuts at: 3058 4426 3058
Size: 1368 5737
AclI AA'CG_TT
Cuts at: 1563 5232 5605 1563
Size: 3669 373 3063
Fragments arranged by size:
3669 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 3053 3676 4291 5168 6552 6820 1632
Size: 1328 93 623 615 877 1384 268 1917
Fragments arranged by size:
1917 1384 1328 877 623 615 268 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6908
AflIII A'CryG_T
Cuts at: 1203 2919 3801 6004 6981 1203
Size: 1716 882 2203 977 1327
Fragments arranged by size:
2203 1716 1327 977 882
AgeI A'CCGG_T
Cuts at: 3177 5936 3177
Size: 2759 4346
AhdI GACnn_n'nnGTC
Cuts at: 5834 5834
Size: 7105
AleI CACnn'nnGTG
Cuts at: 1166 2201 1166
Size: 1035 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 3695 1184
Size: 1143 1368 4594
Fragments arranged by size:
4594 1368 1143
AlwNI CAG_nnn'CTG
Cuts at: 2437 2836 2848 2881 3589 3592 3968 4848 6420
Size: 399 12 33 708 3 376 880 1572
Cuts at: 6420 2437
Size: 3122
Fragments arranged by size:
3122 1572 880 708 399 376 33 12
3
ApaI G_GGCC'C
Cuts at: 2216 2952 3641 2216
Size: 736 689 5680
Fragments arranged by size:
5680 736 689
ApaLI G'TGCA_C
Cuts at: 5162 6318 5162
Size: 1156 5949
ApoI r'AATT_y
Cuts at: 1493 4414 4640 4792 6901 1493
Size: 2921 226 152 2109 1697
Fragments arranged by size:
2921 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5659 160
Size: 872 4627 1606
Fragments arranged by size:
4627 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 2931 3530 4031 4430 1870
Size: 73 273 715 599 501 399 4545
Fragments arranged by size:
4545 715 599 501 399 273 73
AvrII C'CTAG_G
Cuts at: 2953 2953
Size: 7105
BamHI G'GATC_C
Cuts at: 2382 3921 4435 4802 2382
Size: 1539 514 367 4685
Fragments arranged by size:
4685 1539 514 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2497 2597 2686 3058
Size: 325 217 627 379 331 100 89 372
Cuts at: 3058 3180 3500 3535 4210 4426 5881 618
Size: 122 320 35 675 216 1455 1842
Fragments arranged by size:
1842 1455 675 627 379 372 331 325
320 217 216 122 100 89 35
BanII G_rGCy'C
Cuts at: 1391 2216 2952 3360 3641 3961 4001 4042 4161
Size: 825 736 408 281 320 40 41 119
Cuts at: 4161 4424 1391
Size: 263 4072
Fragments arranged by size:
4072 825 736 408 320 281 263 119
41 40
BbeI G_GCGC'C
Cuts at: 2170 2170
Size: 7105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5296 5330 1903
Size: 34 3359 34 3678
Fragments arranged by size:
3678 3359 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4997 6207 4997
Size: 1210 5895
BclI T'GATC_A
Cuts at: 1548 2025 1548
Size: 477 6628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2684 2816 3851
Size: 107 122 71 631 605 1012 132 1035
Cuts at: 3851 4091 4100 5716 136
Size: 240 9 1616 1525
Fragments arranged by size:
1616 1525 1035 1012 631 605 240 132
122 107 71 9
BglII A'GATC_T
Cuts at: 7100 7100
Size: 7105
BlpI GC'TnA_GC
Cuts at: 4522 4522
Size: 7105
Bme1580I G_kGCm'C
Cuts at: 1790 2216 2554 2689 2952 3641 4215 5166 6322
Size: 426 338 135 263 689 574 951 1156
Cuts at: 6322 1790
Size: 2573
Fragments arranged by size:
2573 1156 951 689 574 426 338 263
135
BmgBI CAC'GTC
Cuts at: 1318 2920 1318
Size: 1602 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2496 2806 3277
Size: 416 53 83 399 24 1079 310 471
Cuts at: 3277 4836 5794 442
Size: 1559 958 1753
Fragments arranged by size:
1753 1559 1079 958 471 416 399 310
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3775 3775
Size: 7105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2315 2680 3431 3671 3730 3935 3945
Size: 1037 39 365 751 240 59 205 10
Cuts at: 3945 5765 1239
Size: 1820 2579
Fragments arranged by size:
2579 1820 1037 751 365 240 205 59
39 10
Bpu10I CC'TnA_GC
Cuts at: 2527 2527
Size: 7105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4184 5230 6095 6393 4184
Size: 1046 865 298 4896
Fragments arranged by size:
4896 1046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 5768 882
Size: 617 575 3694 2219
Fragments arranged by size:
3694 2219 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 3802 4402 4896 493
Size: 711 2598 600 494 2702
Fragments arranged by size:
2702 2598 711 600 494
BsaBI GATnn'nnATC
Cuts at: 7099 7099
Size: 7105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2138 2167 2229 4191
Size: 53 83 186 936 605 29 62 1962
Cuts at: 4191 4912 5294 5929 275
Size: 721 382 635 1451
Fragments arranged by size:
1962 1451 936 721 635 605 382 186
83 62 53 29
BsaWI w'CCGG_w
Cuts at: 3177 3258 5538 5936 6210 6357 6768 3177
Size: 81 2280 398 274 147 411 3514
Fragments arranged by size:
3514 2280 411 398 274 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6880
Fragments arranged by size:
6880 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2921 3248 3368 3845 1186
Size: 1735 327 120 477 4446
Fragments arranged by size:
4446 1735 477 327 120
BseYI C'CCAG_C
Cuts at: 2829 2848 3762 4263 6308 6804 2829
Size: 19 914 501 2045 496 3130
Fragments arranged by size:
3130 2045 914 501 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2318 2441 2973 2987 3233 4105
Size: 573 561 21 123 532 14 246 872
Cuts at: 4105 1163
Size: 4163
Fragments arranged by size:
4163 872 573 561 532 246 123 21
14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4555 5316 6344 1540
Size: 275 186 2554 761 1028 2301
Fragments arranged by size:
2554 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2609 3360 3961 5166 5251 6322 2609
Size: 751 601 1205 85 1071 3392
Fragments arranged by size:
3392 1205 1071 751 601 85
BsmI GAATG_Cn'
Cuts at: 4616 4709 4616
Size: 93 7012
BsmBI CGTCTCn'nnnn_
Cuts at: 2145 2410 3359 2145
Size: 265 949 5891
Fragments arranged by size:
5891 949 265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2554 2609 2689 2952 3360 3641
Size: 399 426 338 55 80 263 408 281
Cuts at: 3641 3961 4001 4042 4161 4215 4424 5166 5251
Size: 320 40 41 119 54 209 742 85
Cuts at: 5251 6322 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 426 408 399 338 320
281 263 209 119 85 80 55 54
41 40
BspEI T'CCGG_A
Cuts at: 6768 6768
Size: 7105
BspHI T'CATG_A
Cuts at: 2781 3300 4994 6724 2781
Size: 519 1694 1730 3162
Fragments arranged by size:
3162 1730 1694 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2340 3226 3908 4139 4198 4293 4460
Size: 1415 81 886 682 231 59 95 167
Cuts at: 4460 844
Size: 3489
Fragments arranged by size:
3489 1415 886 682 231 167 95 81
59
BsrBI CCG'CTC
Cuts at: 1394 1823 2380 3085 3919 4992 1394
Size: 429 557 705 834 1073 3507
Fragments arranged by size:
3507 1073 834 705 557 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5600 5774 65
Size: 1183 4352 174 1396
Fragments arranged by size:
4352 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 2366 2478 3177 4316 4338 5749
Size: 110 511 45 112 699 1139 22 1411
Cuts at: 5749 5936 1700
Size: 187 2869
Fragments arranged by size:
2869 1411 1139 699 511 187 112 110
45 22
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7105
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7105
BssSI C'ACGA_G
Cuts at: 3230 3719 5165 6177 3230
Size: 489 1446 1012 4158
Fragments arranged by size:
4158 1446 1012 489
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2604 3592 4087 794
Size: 1810 988 495 3812
Fragments arranged by size:
3812 1810 988 495
BstBI TT'CG_AA
Cuts at: 2007 5909 2007
Size: 3902 3203
BstEII G'GTnAC_C
Cuts at: 1184 2071 1184
Size: 887 6218
BstXI CCAn_nnnn'nTGG
Cuts at: 3406 3406
Size: 7105
BstYI r'GATC_y
Cuts at: 1972 2382 2433 2877 3492 3921 4435 4802 5187
Size: 410 51 444 615 429 514 367 385
Cuts at: 5187 5204 6656 6742 6754 7100 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 615 514 444 429 410 385
367 346 86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2224 3007 3127 3411 3496 3556 4141
Size: 552 1159 783 120 284 85 60 585
Cuts at: 4141 513
Size: 3477
Fragments arranged by size:
3477 1159 783 585 552 284 120 85
60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2398 3643 487
Size: 683 56 1172 1245 3949
Fragments arranged by size:
3949 1245 1172 683 56
BtsI GCAGTG_nn'
Cuts at: 2311 2975 4621 5415 5435 2311
Size: 664 1646 794 20 3981
Fragments arranged by size:
3981 1646 794 664 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7070
DraI TTT'AAA
Cuts at: 4409 4409
Size: 7105
DraIII CAC_nnn'GTG
Cuts at: 2504 2604 2504
Size: 100 7005
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2908 6112 809
Size: 507 1592 3204 1802
Fragments arranged by size:
3204 1802 1592 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 3408 4101
Size: 54 1425 50 275 18 456 1122 693
Cuts at: 4101 4270 4552 5441 7070 8
Size: 169 282 889 1629 43
Fragments arranged by size:
1629 1425 1122 889 693 456 282 275
169 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4552 1537
Size: 275 2740 4090
Fragments arranged by size:
4090 2740 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3614 4310 5035 1353
Size: 2261 696 725 3423
Fragments arranged by size:
3423 2261 725 696
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2990 5692 6066 6212 387
Size: 1064 138 1401 2702 374 146 1280
Fragments arranged by size:
2702 1401 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3358 3959 3358
Size: 601 6504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2315 2680 2960 3053 3431 3671
Size: 393 644 39 365 280 93 378 240
Cuts at: 3671 3676 3730 3935 3945 4291 5168 5765 6552
Size: 5 54 205 10 346 877 597 787
Cuts at: 6552 6820 1239
Size: 268 1524
Fragments arranged by size:
1524 877 787 644 597 393 378 365
346 280 268 240 205 93 54 39
10 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2627 2903 3977 4289 2627
Size: 276 1074 312 5443
Fragments arranged by size:
5443 1074 312 276
EcoO109I rG'GnC_Cy
Cuts at: 2212 2213 2935 2949 3637 3638 4065 4116 2212
Size: 1 722 14 688 1 427 51 5201
Fragments arranged by size:
5201 722 688 427 51 14 1 1
EcoRI G'AATT_C
Cuts at: 4414 4792 4414
Size: 378 6727
FseI GG_CCGG'CC
Cuts at: 2370 2370
Size: 7105
FspI TGC'GCA
Cuts at: 2237 3596 5611 2237
Size: 1359 2015 3731
Fragments arranged by size:
3731 2015 1359
HaeII r_GCGC'y
Cuts at: 1678 2170 2822 2836 6252 1678
Size: 492 652 14 3416 2531
Fragments arranged by size:
3416 2531 652 492 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2055 2087 2125 2157 5746
Size: 32 135 32 483 32 38 32 3589
Cuts at: 5746 5778 5820 5852 1373
Size: 32 42 32 2626
Fragments arranged by size:
3589 2626 483 135 42 38 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 3814 4449 4695 669
Size: 988 281 97 1779 635 246 3079
Fragments arranged by size:
3079 1779 988 635 281 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7105
HpaI GTT'AAC
Cuts at: 4695 4695
Size: 7105
KasI G'GCGC_C
Cuts at: 2166 2166
Size: 7105
KpnI G_GTAC'C
Cuts at: 3062 4430 3062
Size: 1368 5737
MfeI C'AATT_G
Cuts at: 4704 4704
Size: 7105
MluI A'CGCG_T
Cuts at: 6981 6981
Size: 7105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 4212 4347 6219 6403 1305
Size: 1128 1779 135 1872 184 2007
Fragments arranged by size:
2007 1872 1779 1128 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 4272 10
Size: 54 1425 343 456 1984 2843
Fragments arranged by size:
2843 1984 1425 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2417 3305 3410 5063 5422 5581
Size: 648 1035 216 888 105 1653 359 159
Cuts at: 5581 518
Size: 2042
Fragments arranged by size:
2042 1653 1035 888 648 359 216 159
105
MspA1I CmG'CkG
Cuts at: 1606 2226 2556 2653 2760 3523 3589 4311 4521
Size: 620 330 97 107 763 66 722 210
Cuts at: 4521 5198 6346 6591 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 763 722 677 620 330 245
210 107 97 66
NaeI GCC'GGC
Cuts at: 2323 2368 2480 4318 4340 2323
Size: 45 112 1838 22 5088
Fragments arranged by size:
5088 1838 112 45 22
NarI GG'CG_CC
Cuts at: 2167 2167
Size: 7105
NcoI C'CATG_G
Cuts at: 513 1065 3007 3496 513
Size: 552 1942 489 4122
Fragments arranged by size:
4122 1942 552 489
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7105
NgoMIV G'CCGG_C
Cuts at: 2321 2366 2478 4316 4338 2321
Size: 45 112 1838 22 5088
Fragments arranged by size:
5088 1838 112 45 22
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7105
NotI GC'GGCC_GC
Cuts at: 4552 4552
Size: 7105
NspI r_CATG'y
Cuts at: 1132 1229 4463 6008 1132
Size: 97 3234 1545 2229
Fragments arranged by size:
3234 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2360 1348
Size: 1012 6093
PciI A'CATG_T
Cuts at: 6004 6004
Size: 7105
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 2836 1108
Size: 1635 93 5377
Fragments arranged by size:
5377 1635 93
PmeI GTTT'AAAC
Cuts at: 4409 4409
Size: 7105
PmlI CAC'GTG
Cuts at: 3802 3802
Size: 7105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5174 5206 6687 6719 5174
Size: 32 1481 32 5560
Fragments arranged by size:
5560 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2935 2935
Size: 7105
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7105
PsiI TTA'TAA
Cuts at: 4675 4675
Size: 7105
PspOMI G'GGCC_C
Cuts at: 2212 2948 3637 2212
Size: 736 689 5680
Fragments arranged by size:
5680 736 689
PspXI vC'TCGA_Gb
Cuts at: 4031 4031
Size: 7105
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4232 4241 4264 4273 4232
Size: 9 23 9 7064
Fragments arranged by size:
7064 23 9 9
PstI C_TGCA'G
Cuts at: 830 3045 3138 3684 3956 4209 4290 4457 830
Size: 2215 93 546 272 253 81 167 3478
Fragments arranged by size:
3478 2215 546 272 253 167 93 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7105
PvuII CAG'CTG
Cuts at: 3589 4311 3589
Size: 722 6383
SacI G_AGCT'C
Cuts at: 3360 3961 3360
Size: 601 6504
SacII CC_GC'GG
Cuts at: 2227 2227
Size: 7105
SalI G'TCGA_C
Cuts at: 1936 3812 4447 1936
Size: 1876 635 4594
Fragments arranged by size:
4594 1876 635
SapI GCTCTTCn'nnn_
Cuts at: 4310 4310
Size: 7105
SbfI CC_TGCA'GG
Cuts at: 4457 4457
Size: 7105
ScaI AGT'ACT
Cuts at: 5353 7065 5353
Size: 1712 5393
SfcI C'TryA_G
Cuts at: 826 1045 3041 3134 3680 3952 4205 4286 4453
Size: 219 1996 93 546 272 253 81 167
Cuts at: 4453 5588 6269 6460 826
Size: 1135 681 191 1471
Fragments arranged by size:
1996 1471 1135 681 546 272 253 219
191 167 93 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 4100 4100
Size: 7105
SfoI GGC'GCC
Cuts at: 2168 2168
Size: 7105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7105
SmaI CCC'GGG
Cuts at: 1872 4432 1872
Size: 2560 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4031 4199 5209 6110 6372 820
Size: 197 926 2088 168 1010 901 262 1553
Fragments arranged by size:
2088 1553 1010 926 901 262 197 168
SnaBI TAC'GTA
Cuts at: 493 4402 4896 493
Size: 3909 494 2702
Fragments arranged by size:
3909 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7105
SphI G_CATG'C
Cuts at: 1132 4463 1132
Size: 3331 3774
SspI AAT'ATT
Cuts at: 5 52 5029 5
Size: 47 4977 2081
Fragments arranged by size:
4977 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7105
StyI C'CwwG_G
Cuts at: 513 1065 2953 3007 3496 4544 513
Size: 552 1888 54 489 1048 3074
Fragments arranged by size:
3074 1888 1048 552 489 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2515 2704 2873 4040 5132 5149 5302
Size: 1255 935 189 169 1167 1092 17 153
Cuts at: 5302 5487 5932 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1167 1092 935 445 189 185
169 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3507 3894 5351
Size: 274 80 33 51 1443 1530 387 1457
Cuts at: 5351 7063 96
Size: 1712 138
Fragments arranged by size:
1712 1530 1457 1443 387 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2982 3014 7065 7097 2982
Size: 32 4051 32 2990
Fragments arranged by size:
4051 2990 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7105
XbaI T'CTAG_A
Cuts at: 4441 4441
Size: 7105
XhoI C'TCGA_G
Cuts at: 1943 4031 1943
Size: 2088 5017
XmaI C'CCGG_G
Cuts at: 1870 4430 1870
Size: 2560 4545
XmnI GAAnn'nnTTC
Cuts at: 5234 5234
Size: 7105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2139 4913 276
Size: 53 83 186 936 605 2774 2468
Fragments arranged by size:
2774 2468 936 605 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FalI FspAI NruI NsiI PacI PfoI
RsrII SanDI SexAI SrfI SwaI XcmI