Restriction map for
pFN21ASDA1994
(Circular) (Six-base) MAPSORT of: pFN21ASDA1994.seq Check: 4905 from: 1 to: 7948
pFN21ASDA1994 7948 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2887 4330 2887
Size: 1443 6505
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5758 278
Size: 53 83 186 936 4222 2468
Fragments arranged by size:
4222 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3625 5291 1937
Size: 1688 1666 4594
Fragments arranged by size:
4594 1688 1666
Acc65I G'GTAC_C
Cuts at: 2156 5269 2156
Size: 3113 4835
AclI AA'CG_TT
Cuts at: 1563 6075 6448 1563
Size: 4512 373 3063
Fragments arranged by size:
4512 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 3692 3771 3966 4260 5123 6011 7395
Size: 566 1494 79 195 294 863 888 1384
Cuts at: 7395 7663 1632
Size: 268 1917
Fragments arranged by size:
1917 1494 1384 888 863 566 294 268
195 79
AfeI AGC'GCT
Cuts at: 2077 2077
Size: 7948
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7751
AflIII A'CryG_T
Cuts at: 1203 6847 7824 1203
Size: 5644 977 1327
Fragments arranged by size:
5644 1327 977
AgeI A'CCGG_T
Cuts at: 6779 6779
Size: 7948
AhdI GACnn_n'nnGTC
Cuts at: 6677 6677
Size: 7948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4494 1184
Size: 3310 4638
AlwNI CAG_nnn'CTG
Cuts at: 4184 4868 5691 7263 4184
Size: 684 823 1572 4869
Fragments arranged by size:
4869 1572 823 684
ApaLI G'TGCA_C
Cuts at: 6005 7161 6005
Size: 1156 6792
ApoI r'AATT_y
Cuts at: 1493 2935 4669 5071 5257 5483 5635 7744 1493
Size: 1442 1734 402 186 226 152 2109 1697
Fragments arranged by size:
2109 1734 1697 1442 402 226 186 152
AseI AT'TA_AT
Cuts at: 160 1032 6502 160
Size: 872 5470 1606
Fragments arranged by size:
5470 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
AvaI C'yCGr_G
Cuts at: 1870 1943 5273 1870
Size: 73 3330 4545
Fragments arranged by size:
4545 3330 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2148 2181 2148
Size: 33 7915
BamHI G'GATC_C
Cuts at: 5278 5645 5278
Size: 367 7581
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2709 3883 5189 5269
Size: 325 217 627 369 553 1174 1306 80
Cuts at: 5269 6724 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1306 1174 627 553 369 325
217 80
BanII G_rGCy'C
Cuts at: 1391 2526 3276 3496 4779 4937 5228 5267 1391
Size: 1135 750 220 1283 158 291 39 4072
Fragments arranged by size:
4072 1283 1135 750 291 220 158 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3698 5111 928
Size: 971 1799 1413 3765
Fragments arranged by size:
3765 1799 1413 971
BbvCI CC'TCA_GC
Cuts at: 2613 2613
Size: 7948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6139 6173 1903
Size: 34 4202 34 3678
Fragments arranged by size:
4202 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4106 5840 7050 3761
Size: 345 1734 1210 4659
Fragments arranged by size:
4659 1734 1210 345
BclI T'GATC_A
Cuts at: 1548 2652 1548
Size: 1104 6844
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6559 136
Size: 107 122 71 631 605 4887 1525
Fragments arranged by size:
4887 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2382 2464 3651 7943 2382
Size: 82 1187 4292 2387
Fragments arranged by size:
4292 2387 1187 82
BlpI GC'TnA_GC
Cuts at: 2295 4083 5365 2295
Size: 1788 1282 4878
Fragments arranged by size:
4878 1788 1282
Bme1580I G_kGCm'C
Cuts at: 1790 2712 3886 5192 6009 7165 1790
Size: 922 1174 1306 817 1156 2573
Fragments arranged by size:
2573 1306 1174 1156 922 817
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2786 3198 4032
Size: 416 53 83 399 24 1369 412 834
Cuts at: 4032 4341 4702 5128 5679 6637 442
Size: 309 361 426 551 958 1753
Fragments arranged by size:
1753 1369 958 834 551 426 416 412
399 361 309 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7948
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3657 4954 3657
Size: 1297 6651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3102 3843 5102 6608 1239
Size: 1863 741 1259 1506 2579
Fragments arranged by size:
2579 1863 1506 1259 741
Bpu10I CC'TnA_GC
Cuts at: 2613 4731 5121 5227 2613
Size: 2118 390 106 5334
Fragments arranged by size:
5334 2118 390 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2770 3133 3660 4437 6073 6938 7236 2770
Size: 363 527 777 1636 865 298 3482
Fragments arranged by size:
3482 1636 865 777 527 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 6611 882
Size: 617 1041 4071 2219
Fragments arranged by size:
4071 2219 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 5245 5739 493
Size: 711 4041 494 2702
Fragments arranged by size:
4041 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7942 7942
Size: 7948
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 5755 6137 6772
Size: 53 83 186 936 630 3592 382 635
Cuts at: 6772 275
Size: 1451
Fragments arranged by size:
3592 1451 936 635 630 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4028 6381 6779 7053 7200 7611 4028
Size: 2353 398 274 147 411 4365
Fragments arranged by size:
4365 2353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2664 2694 3540 3570 4348
Size: 30 165 30 1261 30 846 30 778
Cuts at: 4348 4378 4405 4435 1178
Size: 30 27 30 4691
Fragments arranged by size:
4691 1261 846 778 165 30 30 30
30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2527 3846 4287 4927 1186
Size: 1341 1319 441 640 4207
Fragments arranged by size:
4207 1341 1319 640 441
BseYI C'CCAG_C
Cuts at: 3831 4126 4425 5076 5145 5178 5208 7151 7647
Size: 295 299 651 69 33 30 1943 496
Cuts at: 7647 3831
Size: 4132
Fragments arranged by size:
4132 1943 651 496 299 295 69 33
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3530 4130 4268 4788 1163
Size: 573 1794 600 138 520 4323
Fragments arranged by size:
4323 1794 600 573 520 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 5398 6159 7187 1540
Size: 275 186 54 3343 761 1028 2301
Fragments arranged by size:
3343 2301 1028 761 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2908 3416 4297 4366 4937 6009 6094 7165 2908
Size: 508 881 69 571 1072 85 1071 3691
Fragments arranged by size:
3691 1072 1071 881 571 508 85 69
BsmI GAATG_Cn'
Cuts at: 2668 4557 5459 5552 2668
Size: 1889 902 93 5064
Fragments arranged by size:
5064 1889 902 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2526 2712 2908 3276 3416 3496 3886
Size: 399 736 186 196 368 140 80 390
Cuts at: 3886 4297 4366 4779 4937 5192 5228 5267 6009
Size: 411 69 413 158 255 36 39 742
Cuts at: 6009 6094 7165 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 413 411 399 390
368 255 196 186 158 140 85 80
69 39 36
BspEI T'CCGG_A
Cuts at: 4028 7611 4028
Size: 3583 4365
BspHI T'CATG_A
Cuts at: 5837 7567 5837
Size: 1730 6218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2144 2887 3340 4144 4330 5303 844
Size: 1300 743 453 804 186 973 3489
Fragments arranged by size:
3489 1300 973 804 743 453 186
BsrBI CCG'CTC
Cuts at: 1394 1823 5835 1394
Size: 429 4012 3507
Fragments arranged by size:
4012 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2106 3133 4026 6443 6617 65
Size: 1183 858 1027 893 2417 174 1396
Fragments arranged by size:
2417 1396 1183 1027 893 858 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 6592 6779 1700
Size: 110 349 4433 187 2869
Fragments arranged by size:
4433 2869 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 3991 96
Size: 3895 4053
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7948
BssSI C'ACGA_G
Cuts at: 6008 7020 6008
Size: 1012 6936
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4136 4184 4868 794
Size: 3342 48 684 3874
Fragments arranged by size:
3874 3342 684 48
BstBI TT'CG_AA
Cuts at: 2007 6752 2007
Size: 4745 3203
BstEII G'GTnAC_C
Cuts at: 1184 2726 1184
Size: 1542 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 4160 4160
Size: 7948
BstYI r'GATC_y
Cuts at: 1972 2382 2464 2481 3651 4987 5278 5645 6030
Size: 410 82 17 1170 1336 291 367 385
Cuts at: 6030 6047 7499 7585 7597 7943 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1336 1170 410 385 367 346
291 86 82 17 17 12
BstZ17I GTA'TAC
Cuts at: 3626 3626
Size: 7948
BtgI C'CryG_G
Cuts at: 513 1065 2049 513
Size: 552 984 6412
Fragments arranged by size:
6412 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3744 5202 487
Size: 683 56 2518 1458 3233
Fragments arranged by size:
3233 2518 1458 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 4185 4251 5464 6258 6278 3166
Size: 1019 66 1213 794 20 4836
Fragments arranged by size:
4836 1213 1019 794 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7913
DraI TTT'AAA
Cuts at: 3280 5252 3280
Size: 1972 5976
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6955 809
Size: 507 5639 1802
Fragments arranged by size:
5639 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2754 4162
Size: 54 1425 50 275 18 222 702 1408
Cuts at: 4162 5112 5395 6284 7913 8
Size: 950 283 889 1629 43
Fragments arranged by size:
1629 1425 1408 950 889 702 283 275
222 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2052 5395 1537
Size: 275 240 3343 4090
Fragments arranged by size:
4090 3343 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 2476 2538 5878 1353
Size: 1123 62 3340 3423
Fragments arranged by size:
3423 3340 1123 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2187 2250 2340 6535 6909 7055
Size: 1064 138 598 63 90 4195 374 146
Cuts at: 7055 387
Size: 1280
Fragments arranged by size:
4195 1280 1064 598 374 146 138 90
63
EcoICRI GAG'CTC
Cuts at: 4935 4935
Size: 7948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 3102 3692 3771 3843 3966 4260
Size: 393 566 904 590 79 72 123 294
Cuts at: 4260 5102 5123 6011 6608 7395 7663 1239
Size: 842 21 888 597 787 268 1524
Fragments arranged by size:
1524 904 888 842 787 597 590 566
393 294 268 123 79 72 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2288
Size: 7948
EcoO109I rG'GnC_Cy
Cuts at: 5035 5035
Size: 7948
EcoRI G'AATT_C
Cuts at: 5257 5635 5257
Size: 378 7570
FspI TGC'GCA
Cuts at: 6454 6454
Size: 7948
HaeII r_GCGC'y
Cuts at: 1678 2079 2136 7095 1678
Size: 401 57 4959 2531
Fragments arranged by size:
4959 2531 401 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3958 3990 4327 4359 4919
Size: 32 135 32 2386 32 337 32 560
Cuts at: 4919 4951 6589 6621 6663 6695 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 2386 1638 560 337 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 3229 4413 4643 5292 5538
Size: 988 281 1033 258 1184 230 649 246
Cuts at: 5538 669
Size: 3079
Fragments arranged by size:
3079 1184 1033 988 649 281 258 246
230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7948
HpaI GTT'AAC
Cuts at: 2971 3229 4413 5538 2971
Size: 258 1184 1125 5381
Fragments arranged by size:
5381 1184 1125 258
KpnI G_GTAC'C
Cuts at: 2160 5273 2160
Size: 3113 4835
MfeI C'AATT_G
Cuts at: 4382 4737 5547 4382
Size: 355 810 6783
Fragments arranged by size:
6783 810 355
MluI A'CGCG_T
Cuts at: 7824 7824
Size: 7948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2743 3598 7062 7246 1305
Size: 1438 855 3464 184 2007
Fragments arranged by size:
3464 2007 1438 855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 4164 5114 10
Size: 54 1425 343 924 1408 950 2844
Fragments arranged by size:
2844 1425 1408 950 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5906 6265 6424 518
Size: 648 4740 359 159 2042
Fragments arranged by size:
4740 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2063 2121 2299 3176 4102 4772 4900
Size: 445 12 58 178 877 926 670 128
Cuts at: 4900 5080 5149 5364 6041 7189 7434 1606
Size: 180 69 215 677 1148 245 2120
Fragments arranged by size:
2120 1148 926 877 677 670 445 245
215 180 178 128 69 58 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7396
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7948
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7948
NotI GC'GGCC_GC
Cuts at: 2052 5395 2052
Size: 3343 4605
NspI r_CATG'y
Cuts at: 1132 1229 5306 6851 1132
Size: 97 4077 1545 2229
Fragments arranged by size:
4077 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7948
PciI A'CATG_T
Cuts at: 6847 6847
Size: 7948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3580 1108
Size: 2472 5476
PfoI T'CCnGG_A
Cuts at: 2562 2562
Size: 7948
PmeI GTTT'AAAC
Cuts at: 5252 5252
Size: 7948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6017 6049 7530 7562 6017
Size: 32 1481 32 6403
Fragments arranged by size:
6403 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5035 5035
Size: 7948
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7948
PsiI TTA'TAA
Cuts at: 5518 5518
Size: 7948
PstI C_TGCA'G
Cuts at: 830 2068 2233 2304 3181 4048 4141 4246 4483
Size: 1238 165 71 877 867 93 105 237
Cuts at: 4483 5154 5300 830
Size: 671 146 3478
Fragments arranged by size:
3478 1238 877 867 671 237 165 146
105 93 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7948
PvuII CAG'CTG
Cuts at: 2299 3176 4102 5080 5149 2299
Size: 877 926 978 69 5098
Fragments arranged by size:
5098 978 926 877 69
SacI G_AGCT'C
Cuts at: 4937 4937
Size: 7948
SacII CC_GC'GG
Cuts at: 2052 2052
Size: 7948
SalI G'TCGA_C
Cuts at: 1936 5290 1936
Size: 3354 4594
SbfI CC_TGCA'GG
Cuts at: 4141 5300 4141
Size: 1159 6789
ScaI AGT'ACT
Cuts at: 2914 6196 7908 2914
Size: 3282 1712 2954
Fragments arranged by size:
3282 2954 1712
SfcI C'TryA_G
Cuts at: 826 1045 2064 2229 2300 2916 3177 3336 4044
Size: 219 1019 165 71 616 261 159 708
Cuts at: 4044 4137 4242 4479 5150 5296 6431 7112 7303
Size: 93 105 237 671 146 1135 681 191
Cuts at: 7303 826
Size: 1471
Fragments arranged by size:
1471 1135 1019 708 681 671 616 261
237 219 191 165 159 146 105 93
71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7948
SmaI CCC'GGG
Cuts at: 1872 5275 1872
Size: 3403 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2785 3112 3675 4452 6052 6953
Size: 197 926 842 327 563 777 1600 901
Cuts at: 6953 7215 820
Size: 262 1553
Fragments arranged by size:
1600 1553 926 901 842 777 563 327
262 197
SnaBI TAC'GTA
Cuts at: 493 5245 5739 493
Size: 4752 494 2702
Fragments arranged by size:
4752 2702 494
SpeI A'CTAG_T
Cuts at: 152 2951 3416 4358 152
Size: 2799 465 942 3742
Fragments arranged by size:
3742 2799 942 465
SphI G_CATG'C
Cuts at: 1132 5306 1132
Size: 4174 3774
SspI AAT'ATT
Cuts at: 5 52 3049 4216 5872 5
Size: 47 2997 1167 1656 2081
Fragments arranged by size:
2997 2081 1656 1167 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7948
StyI C'CwwG_G
Cuts at: 513 1065 3019 3559 5387 513
Size: 552 1954 540 1828 3074
Fragments arranged by size:
3074 1954 1828 552 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5192 5975 5992 6145 6330 6775 325
Size: 1255 3612 783 17 153 185 445 1498
Fragments arranged by size:
3612 1498 1255 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2912 2954 3038
Size: 274 80 33 51 1443 935 42 84
Cuts at: 3038 3661 3991 6194 7906 96
Size: 623 330 2203 1712 138
Fragments arranged by size:
2203 1712 1443 935 623 330 274 138
84 80 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2664 2696 4053 4080 4085 4112 7908 7940 2664
Size: 32 1357 27 5 27 3796 32 2672
Fragments arranged by size:
3796 2672 1357 32 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7948
XbaI T'CTAG_A
Cuts at: 2798 5284 2798
Size: 2486 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2720 3817 5153 2720
Size: 1097 1336 5515
Fragments arranged by size:
5515 1336 1097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7948
XmaI C'CCGG_G
Cuts at: 1870 5273 1870
Size: 3403 4545
XmnI GAAnn'nnTTC
Cuts at: 4462 4555 6077 4462
Size: 93 1522 6333
Fragments arranged by size:
6333 1522 93
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5756 276
Size: 53 83 186 936 4222 2468
Fragments arranged by size:
4222 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI