Restriction map for
pFN21ASDA1993
(Circular) (Six-base) MAPSORT of: pFN21ASDA1993.seq Check: 6264 from: 1 to: 6241
pFN21ASDA1993 6241 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3030 3030
Size: 6241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 2802 3129 3356 3562 2802
Size: 327 227 206 5481
Fragments arranged by size:
5481 327 227 206
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2345 2478 3420 4304 5688 5956 1632
Size: 710 3 133 942 884 1384 268 1917
Fragments arranged by size:
1917 1384 942 884 710 268 133 3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 2732 1166
Size: 1566 4675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2618 1184
Size: 1434 4807
AlwNI CAG_nnn'CTG
Cuts at: 3043 3521 3984 5556 3043
Size: 478 463 1572 3728
Fragments arranged by size:
3728 1572 478 463
ApaLI G'TGCA_C
Cuts at: 2386 4298 5454 2386
Size: 1912 1156 3173
Fragments arranged by size:
3173 1912 1156
ApoI r'AATT_y
Cuts at: 1493 3507 3550 3776 3928 6037 1493
Size: 2014 43 226 152 2109 1697
Fragments arranged by size:
2109 2014 1697 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2468 3083 3102 3425 3566 1870
Size: 73 126 399 615 19 323 141 4545
Fragments arranged by size:
4545 615 399 323 141 126 73 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2901 2934 3266 3299 2901
Size: 33 332 33 5843
Fragments arranged by size:
5843 332 33 33
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2802 3129 3356 3562 5017
Size: 325 217 627 1015 327 227 206 1455
Cuts at: 5017 618
Size: 1842
Fragments arranged by size:
1842 1455 1015 627 327 325 227 217
206
BanII G_rGCy'C
Cuts at: 1391 2170 2855 3056 3560 1391
Size: 779 685 201 504 4072
Fragments arranged by size:
4072 779 685 504 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2520 3327 6236 2520
Size: 807 2909 2525
Fragments arranged by size:
2909 2525 807
BlpI GC'TnA_GC
Cuts at: 2969 3658 2969
Size: 689 5552
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2390 2631 2995 4302 5458 1790
Size: 280 320 241 364 1307 1156 2573
Fragments arranged by size:
2573 1307 1156 364 320 280 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2507 3972 4930
Size: 416 53 83 399 24 1090 1465 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
1753 1465 1090 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 3299 4901 1239
Size: 1616 444 1602 2579
Fragments arranged by size:
2579 1616 1602 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4366 5231 5529 4366
Size: 865 298 5078
Fragments arranged by size:
5078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2679 2694 4904 882
Size: 617 1180 15 2210 2219
Fragments arranged by size:
2219 2210 1180 617 15
BsaAI yAC'GTr
Cuts at: 493 1204 3445 3538 4032 493
Size: 711 2241 93 494 2702
Fragments arranged by size:
2702 2241 711 494 93
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4048 4430 5065 275
Size: 53 83 186 936 2515 382 635 1451
Fragments arranged by size:
2515 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2574 4674 5072 5346 5493 5904 2574
Size: 2100 398 274 147 411 2911
Fragments arranged by size:
2911 2100 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2768 2798 2867 2897 1178
Size: 30 165 30 1365 30 69 30 4522
Fragments arranged by size:
4522 1365 165 69 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2517 2636 2773 2997 3124 1186
Size: 1331 119 137 224 127 4303
Fragments arranged by size:
4303 1331 224 137 127 119
BseYI C'CCAG_C
Cuts at: 2591 2765 5444 5940 2591
Size: 174 2679 496 2892
Fragments arranged by size:
2892 2679 496 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3008 3068 1163
Size: 573 1272 60 4336
Fragments arranged by size:
4336 1272 573 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3691 4452 5480 1540
Size: 275 186 760 930 761 1028 2301
Fragments arranged by size:
2301 1028 930 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 2390 2871 4302 4387 5458 2390
Size: 481 1431 85 1071 3173
Fragments arranged by size:
3173 1431 1071 481 85
BsmI GAATG_Cn'
Cuts at: 3752 3845 3752
Size: 93 6148
BsmBI CGTCTCn'nnnn_
Cuts at: 2576 2576
Size: 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2170 2390 2631 2855 2871 2995
Size: 399 280 100 220 241 224 16 124
Cuts at: 2995 3056 3560 4302 4387 5458 1391
Size: 61 504 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 280 241 224
220 124 100 85 61 16
BspEI T'CCGG_A
Cuts at: 2574 5904 2574
Size: 3330 2911
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3030 3042 3596 844
Size: 2186 12 554 3489
Fragments arranged by size:
3489 2186 554 12
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4736 4910 65
Size: 1183 3488 174 1396
Fragments arranged by size:
3488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 4885 5072 1700
Size: 110 950 2125 187 2869
Fragments arranged by size:
2869 2125 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 2140 96
Size: 2044 4197
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2183 794
Size: 1389 4852
BstBI TT'CG_AA
Cuts at: 2007 5045 2007
Size: 3038 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 2394 2520 3327 3571 3938 4323 4340 5792
Size: 422 126 807 244 367 385 17 1452
Cuts at: 5792 5878 5890 6236 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 807 422 385 367 346 244
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2638 513
Size: 552 1573 4116
Fragments arranged by size:
4116 1573 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5502
Fragments arranged by size:
5502 683 56
BtsI GCAGTG_nn'
Cuts at: 2375 2572 2653 2847 3757 4551 4571 2375
Size: 197 81 194 910 794 20 4045
Fragments arranged by size:
4045 910 794 197 194 81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 529
Size: 35 2665 35 3506
Fragments arranged by size:
3506 2665 35 35
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DraIII CAC_nnn'GTG
Cuts at: 3517 3517
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3688 4577 6206
Size: 54 1425 50 275 18 1858 889 1629
Cuts at: 6206 8
Size: 43
Fragments arranged by size:
1858 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 4171 1353
Size: 1101 1717 3423
Fragments arranged by size:
3423 1717 1101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 4828 5202 5348 387
Size: 1064 138 510 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 510 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2345 2478 2855 3299 3420 4304
Size: 393 710 3 133 377 444 121 884
Cuts at: 4304 4901 5688 5956 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 884 787 710 597 444 393 377
268 133 121 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3047 3047
Size: 6241
EcoO109I rG'GnC_Cy
Cuts at: 3040 3040
Size: 6241
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2198 3209 2198
Size: 1011 5230
FspI TGC'GCA
Cuts at: 2606 4747 2606
Size: 2141 4100
HaeII r_GCGC'y
Cuts at: 1678 5388 1678
Size: 3710 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2245 2277 2423 2455 2512
Size: 32 135 32 673 32 146 32 57
Cuts at: 2512 2544 2768 2800 4882 4914 4956 4988 1373
Size: 32 224 32 2082 32 42 32 2626
Fragments arranged by size:
2626 2082 673 224 146 135 57 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 3585 3831 669
Size: 988 281 499 1148 246 3079
Fragments arranged by size:
3079 1148 988 499 281 246
HindIII A'AGCT_T
Cuts at: 748 2598 2925 748
Size: 1850 327 4064
Fragments arranged by size:
4064 1850 327
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KpnI G_GTAC'C
Cuts at: 2806 3133 3360 3566 2806
Size: 327 227 206 5481
Fragments arranged by size:
5481 327 227 206
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2407 2781 2799 5355 5539 1305
Size: 1102 374 18 2556 184 2007
Fragments arranged by size:
2556 2007 1102 374 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4419
Fragments arranged by size:
4419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 2813 3303 4199 4558 4717 518
Size: 648 1566 81 490 896 359 159 2042
Fragments arranged by size:
2042 1566 896 648 490 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2320 2640 2769 3321 3657 4334 5482 5727
Size: 714 320 129 552 336 677 1148 245
Cuts at: 5727 1606
Size: 2120
Fragments arranged by size:
2120 1148 714 677 552 336 320 245
129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5689
NdeI CA'TA_TG
Cuts at: 387 3216 387
Size: 2829 3412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 3208 3599 5144 1132
Size: 97 1979 391 1545 2229
Fragments arranged by size:
2229 1979 1545 391 97
PasI CC'CwG_GG
Cuts at: 1348 2788 1348
Size: 1440 4801
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6241
PfoI T'CCnGG_A
Cuts at: 2205 3497 2205
Size: 1292 4949
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2118 2350 3039 3593 830
Size: 1288 232 689 554 3478
Fragments arranged by size:
3478 1288 689 554 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2320 2769 2320
Size: 449 5792
SacII CC_GC'GG
Cuts at: 2641 2641
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SbfI CC_TGCA'GG
Cuts at: 2118 3039 3593 2118
Size: 921 554 4766
Fragments arranged by size:
4766 921 554
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2114 2346 3035 3589 4724 5405 5596
Size: 219 1069 232 689 554 1135 681 191
Cuts at: 5596 826
Size: 1471
Fragments arranged by size:
1471 1135 1069 689 681 554 232 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3085 3104 3568 1872
Size: 1213 19 464 4545
Fragments arranged by size:
4545 1213 464 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4345 5246 5508 820
Size: 197 926 2402 901 262 1553
Fragments arranged by size:
2402 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3538 4032 493
Size: 3045 494 2702
Fragments arranged by size:
3045 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6241
StyI C'CwwG_G
Cuts at: 513 1065 3352 3680 513
Size: 552 2287 328 3074
Fragments arranged by size:
3074 2287 552 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2780 3402 4268 4285 4438 4623 5068
Size: 1255 1200 622 866 17 153 185 445
Cuts at: 5068 325
Size: 1498
Fragments arranged by size:
1498 1255 1200 866 622 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2140 3258
Size: 274 80 33 51 1443 96 67 1118
Cuts at: 3258 4487 6199 96
Size: 1229 1712 138
Fragments arranged by size:
1712 1443 1229 1118 274 138 96 80
67 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2113 2145 2867 2899 6201 6233 2113
Size: 32 722 32 3302 32 2121
Fragments arranged by size:
3302 2121 722 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6241
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3083 3102 3566 1870
Size: 1213 19 464 4545
Fragments arranged by size:
4545 1213 464 19
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI