Restriction map for
pFN21ASDA1972
(Circular) (Six-base) MAPSORT of: pFN21ASDA1972.seq Check: 7752 from: 1 to: 6457
pFN21ASDA1972 6457 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2938 3800 1937
Size: 1001 862 4594
Fragments arranged by size:
4594 1001 862
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2744 3035 3549 4520 5904 6172 1632
Size: 1112 291 514 971 1384 268 1917
Fragments arranged by size:
1917 1384 1112 971 514 291 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 5356 6333 1203
Size: 4153 977 1327
Fragments arranged by size:
4153 1327 977
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3380 1184
Size: 2196 4261
AlwNI CAG_nnn'CTG
Cuts at: 2077 2669 2722 4200 5772 2077
Size: 592 53 1478 1572 2762
Fragments arranged by size:
2762 1572 1478 592 53
ApaLI G'TGCA_C
Cuts at: 4514 5670 4514
Size: 1156 5301
ApoI r'AATT_y
Cuts at: 1493 2562 3170 3491 3766 3992 4144 6253 1493
Size: 1069 608 321 275 226 152 2109 1697
Fragments arranged by size:
2109 1697 1069 608 321 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 3159 3782 1870
Size: 73 1216 623 4545
Fragments arranged by size:
4545 1216 623 73
AvrII C'CTAG_G
Cuts at: 2113 2638 2113
Size: 525 5932
BamHI G'GATC_C
Cuts at: 3787 4154 3787
Size: 367 6090
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 3778 5233 618
Size: 325 217 627 339 1652 1455 1842
Fragments arranged by size:
1842 1652 1455 627 339 325 217
BanII G_rGCy'C
Cuts at: 1391 3776 1391
Size: 2385 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2201 2235 3579 3613 4648 4682 1903
Size: 34 264 34 1344 34 1035 34 3678
Fragments arranged by size:
3678 1344 1035 264 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4349 5559 3596
Size: 753 1210 4494
Fragments arranged by size:
4494 1210 753
BclI T'GATC_A
Cuts at: 1548 2374 1548
Size: 826 5631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5068 136
Size: 107 122 71 631 605 3396 1525
Fragments arranged by size:
3396 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3018 6452 3018
Size: 3434 3023
BlpI GC'TnA_GC
Cuts at: 3874 3874
Size: 6457
Bme1580I G_kGCm'C
Cuts at: 1790 2902 4518 5674 1790
Size: 1112 1616 1156 2573
Fragments arranged by size:
2573 1616 1156 1112
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 2896 4188
Size: 416 53 83 399 24 1078 401 1292
Cuts at: 4188 5146 442
Size: 958 1753
Fragments arranged by size:
1753 1292 1078 958 416 401 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2531 3707 5117 1239
Size: 903 389 1176 1410 2579
Fragments arranged by size:
2579 1410 1176 903 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2584 2986 4582 5447 5745 2584
Size: 402 1596 865 298 3296
Fragments arranged by size:
3296 1596 865 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3683 5120 882
Size: 617 2184 1437 2219
Fragments arranged by size:
2219 2184 1437 617
BsaAI yAC'GTr
Cuts at: 493 1204 3754 4248 493
Size: 711 2550 494 2702
Fragments arranged by size:
2702 2550 711 494
BsaBI GATnn'nnATC
Cuts at: 2373 6451 2373
Size: 4078 2379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3135 3559 4264 4646
Size: 53 83 186 936 1602 424 705 382
Cuts at: 4646 5281 275
Size: 635 1451
Fragments arranged by size:
1602 1451 936 705 635 424 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4890 5288 5562 5709 6120 4890
Size: 398 274 147 411 5227
Fragments arranged by size:
5227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3288 3318 1178
Size: 30 165 30 1885 30 4317
Fragments arranged by size:
4317 1885 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3046 1186
Size: 1860 4597
BseYI C'CCAG_C
Cuts at: 3150 5660 6156 3150
Size: 2510 496 3451
Fragments arranged by size:
3451 2510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2241 1163
Size: 573 505 5379
Fragments arranged by size:
5379 573 505
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3907 4668 5696 1540
Size: 275 186 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 4518 4603 5674 2100
Size: 2418 85 1071 2883
Fragments arranged by size:
2883 2418 1071 85
BsmI GAATG_Cn'
Cuts at: 3968 4061 3968
Size: 93 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2902 3776 4518 4603 5674 1391
Size: 399 310 802 874 742 85 1071 2174
Fragments arranged by size:
2174 1071 874 802 742 399 310 85
BspEI T'CCGG_A
Cuts at: 6120 6120
Size: 6457
BspHI T'CATG_A
Cuts at: 2293 3181 4346 6076 2293
Size: 888 1165 1730 2674
Fragments arranged by size:
2674 1730 1165 888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 3701 3812 844
Size: 1810 1047 111 3489
Fragments arranged by size:
3489 1810 1047 111
BsrBI CCG'CTC
Cuts at: 1394 1823 4344 1394
Size: 429 2521 3507
Fragments arranged by size:
3507 2521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2525 3001 4952 5126 65
Size: 1183 1277 476 1951 174 1396
Fragments arranged by size:
1951 1396 1277 1183 476 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2832 3234 5101 5288 1700
Size: 110 1022 402 1867 187 2869
Fragments arranged by size:
2869 1867 1022 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2669 794
Size: 1875 4582
BstBI TT'CG_AA
Cuts at: 2007 5261 2007
Size: 3254 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6457
BstYI r'GATC_y
Cuts at: 1972 2479 3018 3787 4154 4539 4556 6008 6094
Size: 507 539 769 367 385 17 1452 86
Cuts at: 6094 6106 6452 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 769 539 507 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2139 2252 513
Size: 552 1074 113 4718
Fragments arranged by size:
4718 1074 552 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5718
Fragments arranged by size:
5718 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 3973 4767 4787 2229
Size: 1744 794 20 3899
Fragments arranged by size:
3899 1744 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6422
DraI TTT'AAA
Cuts at: 2574 2928 3351 3761 2574
Size: 354 423 410 5270
Fragments arranged by size:
5270 423 410 354
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 5464 809
Size: 507 1521 2627 1802
Fragments arranged by size:
2627 1802 1521 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2267 2649
Size: 54 1425 50 275 18 238 199 382
Cuts at: 2649 3904 4793 6422 8
Size: 1255 889 1629 43
Fragments arranged by size:
1629 1425 1255 889 382 275 238 199
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3904 1537
Size: 275 2092 4090
Fragments arranged by size:
4090 2092 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 4387 1353
Size: 735 2299 3423
Fragments arranged by size:
3423 2299 735
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5044 5418 5564 387
Size: 1064 138 3455 374 146 1280
Fragments arranged by size:
3455 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2531 2744 3035 3549 3707 4520
Size: 393 510 389 213 291 514 158 813
Cuts at: 4520 5117 5904 6172 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 813 787 597 514 510 393 389
291 268 213 158
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2069
Size: 6457
EcoO109I rG'GnC_Cy
Cuts at: 2257 2288 2666 2257
Size: 31 378 6048
Fragments arranged by size:
6048 378 31
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 6457
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 5604 1678
Size: 3926 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3010 3041 3042 3073 3197
Size: 32 135 32 1438 31 1 31 124
Cuts at: 3197 3229 5098 5130 5172 5204 1373
Size: 32 1869 32 42 32 2626
Fragments arranged by size:
2626 1869 1438 135 124 42 32 32
32 32 32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2085 2191 3801 4047 669
Size: 988 281 147 106 1610 246 3079
Fragments arranged by size:
3079 1610 988 281 246 147 106
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6457
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 3635 4056 3635
Size: 421 6036
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 2418 5571 5755 1305
Size: 1011 102 3153 184 2007
Fragments arranged by size:
3153 2007 1011 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2070 10
Size: 54 1425 343 238 4397
Fragments arranged by size:
4397 1425 343 238 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 4415 4774 4933 518
Size: 648 1581 1668 359 159 2042
Fragments arranged by size:
2042 1668 1581 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2495 2944 3241 3364 3527 3685 3873 4550
Size: 889 449 297 123 163 158 188 677
Cuts at: 4550 5698 5943 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 889 677 449 297 245 188
163 158 123
NaeI GCC'GGC
Cuts at: 3236 3236
Size: 6457
NcoI C'CATG_G
Cuts at: 513 1065 2139 513
Size: 552 1074 4831
Fragments arranged by size:
4831 1074 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NgoMIV G'CCGG_C
Cuts at: 3234 3234
Size: 6457
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 3815 5360 1132
Size: 97 2586 1545 2229
Fragments arranged by size:
2586 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6457
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6457
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2257 2288 2666 2257
Size: 31 378 6048
Fragments arranged by size:
6048 378 31
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6457
PsiI TTA'TAA
Cuts at: 2501 4027 2501
Size: 1526 4931
PstI C_TGCA'G
Cuts at: 830 2635 3418 3809 830
Size: 1805 783 391 3478
Fragments arranged by size:
3478 1805 783 391
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
PvuII CAG'CTG
Cuts at: 2495 2944 3241 3364 3527 3685 2495
Size: 449 297 123 163 158 5267
Fragments arranged by size:
5267 449 297 163 158 123
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SanDI GG'GwC_CC
Cuts at: 2257 2257
Size: 6457
SapI GCTCTTCn'nnn_
Cuts at: 2088 2088
Size: 6457
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SfcI C'TryA_G
Cuts at: 826 1045 2631 2714 3414 3805 4940 5621 5812
Size: 219 1586 83 700 391 1135 681 191
Cuts at: 5812 826
Size: 1471
Fragments arranged by size:
1586 1471 1135 700 681 391 219 191
83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 3784 1872
Size: 1912 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2599 2965 3159 4561 5462 5724
Size: 197 926 656 366 194 1402 901 262
Cuts at: 5724 820
Size: 1553
Fragments arranged by size:
1553 1402 926 901 656 366 262 197
194
SnaBI TAC'GTA
Cuts at: 493 3754 4248 493
Size: 3261 494 2702
Fragments arranged by size:
3261 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6457
StyI C'CwwG_G
Cuts at: 513 1065 2065 2113 2139 2273 2300 2638 3548
Size: 552 1000 48 26 134 27 338 910
Cuts at: 3548 3896 513
Size: 348 3074
Fragments arranged by size:
3074 1000 910 552 348 338 134 48
27 26
SwaI ATTT'AAAT
Cuts at: 2574 2574
Size: 6457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2210 3294 4484 4501 4654 4839 5284
Size: 1255 630 1084 1190 17 153 185 445
Cuts at: 5284 325
Size: 1498
Fragments arranged by size:
1498 1255 1190 1084 630 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4703 6415 96
Size: 274 80 33 51 1443 2726 1712 138
Fragments arranged by size:
2726 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2110 2142 6417 6449 2110
Size: 32 4275 32 2118
Fragments arranged by size:
4275 2118 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6457
XbaI T'CTAG_A
Cuts at: 3722 3793 3722
Size: 71 6386
XhoI C'TCGA_G
Cuts at: 1943 3159 1943
Size: 1216 5241
XmaI C'CCGG_G
Cuts at: 1870 3782 1870
Size: 1912 4545
XmnI GAAnn'nnTTC
Cuts at: 4586 4586
Size: 6457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI XcmI