Restriction map for
pFN21ASDA1971
(Circular) (Six-base) MAPSORT of: pFN21ASDA1971.seq Check: 2872 from: 1 to: 7156
pFN21ASDA1971 7156 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4108 4108
Size: 7156
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4966 278
Size: 53 83 186 936 3430 2468
Fragments arranged by size:
3430 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4499 1937
Size: 2562 4594
Acc65I G'GTAC_C
Cuts at: 4477 4477
Size: 7156
AclI AA'CG_TT
Cuts at: 1563 2679 5283 5656 1563
Size: 1116 2604 373 3063
Fragments arranged by size:
3063 2604 1116 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2886 2907 3023 3543 4301 5219 6603 6871
Size: 1254 21 116 520 758 918 1384 268
Cuts at: 6871 1632
Size: 1917
Fragments arranged by size:
1917 1384 1254 918 758 520 268 116
21
AfeI AGC'GCT
Cuts at: 2330 2330
Size: 7156
AflII C'TTAA_G
Cuts at: 820 1017 4171 820
Size: 197 3154 3805
Fragments arranged by size:
3805 3154 197
AflIII A'CryG_T
Cuts at: 1203 6055 7032 1203
Size: 4852 977 1327
Fragments arranged by size:
4852 1327 977
AgeI A'CCGG_T
Cuts at: 5987 5987
Size: 7156
AhdI GACnn_n'nnGTC
Cuts at: 5885 5885
Size: 7156
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7156
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 2126
Size: 32 7124
AlwNI CAG_nnn'CTG
Cuts at: 2455 3424 3447 3707 4899 6471 2455
Size: 969 23 260 1192 1572 3140
Fragments arranged by size:
3140 1572 1192 969 260 23
ApaI G_GGCC'C
Cuts at: 2200 2211 2200
Size: 11 7145
ApaLI G'TGCA_C
Cuts at: 5213 6369 5213
Size: 1156 6000
ApoI r'AATT_y
Cuts at: 1493 3114 3195 3221 3385 4465 4691 4843 6952
Size: 1621 81 26 164 1080 226 152 2109
Cuts at: 6952 1493
Size: 1697
Fragments arranged by size:
2109 1697 1621 1080 226 164 152 81
26
AseI AT'TA_AT
Cuts at: 160 1032 2672 5710 160
Size: 872 1640 3038 1606
Fragments arranged by size:
3038 1640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7156
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2210 2216 2393 3054 4481 1870
Size: 73 147 120 6 177 661 1427 4545
Fragments arranged by size:
4545 1427 661 177 147 120 73 6
AvrII C'CTAG_G
Cuts at: 2827 2827
Size: 7156
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2149 2182 3529 3562 2149
Size: 33 1347 33 5743
Fragments arranged by size:
5743 1347 33 33
BamHI G'GATC_C
Cuts at: 4486 4853 4486
Size: 367 6789
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2097 2507 4477 5932
Size: 325 217 627 272 38 410 1970 1455
Cuts at: 5932 618
Size: 1842
Fragments arranged by size:
1970 1842 1455 627 410 325 272 217
38
BanII G_rGCy'C
Cuts at: 1391 2091 2200 2211 2217 2223 2283 2394 4057
Size: 700 109 11 6 6 60 111 1663
Cuts at: 4057 4189 4475 1391
Size: 132 286 4072
Fragments arranged by size:
4072 1663 700 286 132 111 109 60
11 6 6
BbeI G_GCGC'C
Cuts at: 2511 2511
Size: 7156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6185
BbvCI CC'TCA_GC
Cuts at: 2538 2538
Size: 7156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4082 4116 5347 5381 1903
Size: 34 2145 34 1231 34 3678
Fragments arranged by size:
3678 2145 1231 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5048 6258 5048
Size: 1210 5946
BclI T'GATC_A
Cuts at: 1548 3898 1548
Size: 2350 4806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 2095 5767
Size: 107 122 71 631 605 380 43 3672
Cuts at: 5767 136
Size: 1525
Fragments arranged by size:
3672 1525 631 605 380 122 107 71
43
BglII A'GATC_T
Cuts at: 3133 7151 3133
Size: 4018 3138
BlpI GC'TnA_GC
Cuts at: 4104 4573 4104
Size: 469 6687
Bme1580I G_kGCm'C
Cuts at: 1790 2200 2211 5217 6373 1790
Size: 410 11 3006 1156 2573
Fragments arranged by size:
3006 2573 1156 410 11
BmgBI CAC'GTC
Cuts at: 1318 2362 4079 1318
Size: 1044 1717 4395
Fragments arranged by size:
4395 1717 1044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4887 5845 442
Size: 416 53 83 399 24 3470 958 1753
Fragments arranged by size:
3470 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3053 1056
Size: 1997 5159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 4208 3482
Size: 726 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2345 2873 3446 4041 4361 5816 1239
Size: 830 276 528 573 595 320 1455 2579
Fragments arranged by size:
2579 1455 830 595 573 528 320 276
Bpu10I CC'TnA_GC
Cuts at: 2538 2538
Size: 7156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4005 4373 5281 6146 6444 4005
Size: 368 908 865 298 4717
Fragments arranged by size:
4717 908 865 368 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5819 882
Size: 617 4320 2219
Fragments arranged by size:
4320 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4115 4453 4947 493
Size: 711 2911 338 494 2702
Fragments arranged by size:
2911 2702 711 494 338
BsaBI GATnn'nnATC
Cuts at: 3649 7150 3649
Size: 3501 3655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2508 4963 5345 5980
Size: 53 83 186 936 975 2455 382 635
Cuts at: 5980 275
Size: 1451
Fragments arranged by size:
2455 1451 975 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3468 4194 5589 5987 6261 6408 6819 3468
Size: 726 1395 398 274 147 411 3805
Fragments arranged by size:
3805 1395 726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 1178
Size: 30 165 30 723 30 6178
Fragments arranged by size:
6178 723 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3605 3936 3939 3942 3984 4179 4372 4424
Size: 2419 331 3 3 42 195 193 52
Cuts at: 4424 1186
Size: 3918
Fragments arranged by size:
3918 2419 331 195 193 52 42 3
3
BseYI C'CCAG_C
Cuts at: 2055 2253 3738 4313 6359 6855 2055
Size: 198 1485 575 2046 496 2356
Fragments arranged by size:
2356 2046 1485 575 496 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6583
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2164 2274 2493 2553 4606 5367
Size: 275 186 163 110 219 60 2053 761
Cuts at: 5367 6395 1540
Size: 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 219 186 163
110 60
BsiHKAI G_wGCw'C
Cuts at: 4006 4189 4248 5217 5302 6373 4006
Size: 183 59 969 85 1071 4789
Fragments arranged by size:
4789 1071 969 183 85 59
BsmI GAATG_Cn'
Cuts at: 2964 4667 4760 2964
Size: 1703 93 5360
Fragments arranged by size:
5360 1703 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2245 2905 2245
Size: 660 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2200 2211 2217 2223 2283 2394
Size: 399 301 109 11 6 6 60 111
Cuts at: 2394 4006 4057 4189 4248 4475 5217 5302 6373
Size: 1612 51 132 59 227 742 85 1071
Cuts at: 6373 1391
Size: 2174
Fragments arranged by size:
2174 1612 1071 742 399 301 227 132
111 109 85 60 59 51 11 6
6
BspEI T'CCGG_A
Cuts at: 3468 4194 6819 3468
Size: 726 2625 3805
Fragments arranged by size:
3805 2625 726
BspHI T'CATG_A
Cuts at: 3072 5045 6775 3072
Size: 1973 1730 3453
Fragments arranged by size:
3453 1973 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3729 4108 4511 844
Size: 2885 379 403 3489
Fragments arranged by size:
3489 2885 403 379
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 5043 1394
Size: 429 761 2459 3507
Fragments arranged by size:
3507 2459 761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5651 5825 65
Size: 1183 4403 174 1396
Fragments arranged by size:
4403 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2100 2258 2295 4210 5800 5987
Size: 110 252 38 158 37 1915 1590 187
Cuts at: 5987 1700
Size: 2869
Fragments arranged by size:
2869 1915 1590 252 187 158 110 38
37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7156
BssHII G'CGCG_C
Cuts at: 1925 2289 2422 2593 2595 1925
Size: 364 133 171 2 6486
Fragments arranged by size:
6486 364 171 133 2
BssSI C'ACGA_G
Cuts at: 5216 6228 5216
Size: 1012 6144
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 794
Size: 1310 5846
BstBI TT'CG_AA
Cuts at: 2007 5960 2007
Size: 3953 3203
BstEII G'GTnAC_C
Cuts at: 1184 2716 1184
Size: 1532 5624
BstXI CCAn_nnnn'nTGG
Cuts at: 2662 4445 2662
Size: 1783 5373
BstYI r'GATC_y
Cuts at: 1972 3133 3444 4145 4486 4853 5238 5255 6707
Size: 1161 311 701 341 367 385 17 1452
Cuts at: 6707 6793 6805 7151 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1161 701 385 367 346 341
311 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2229 2229
Size: 7156
BtgI C'CryG_G
Cuts at: 513 1065 2022 2274 2284 2339 2661 2721 513
Size: 552 957 252 10 55 322 60 4948
Fragments arranged by size:
4948 957 552 322 252 60 55 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6417
Fragments arranged by size:
6417 683 56
BtsI GCAGTG_nn'
Cuts at: 3419 3932 4672 5466 5486 3419
Size: 513 740 794 20 5089
Fragments arranged by size:
5089 794 740 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7121
DraI TTT'AAA
Cuts at: 3096 3658 4460 3096
Size: 562 802 5792
Fragments arranged by size:
5792 802 562
DraIII CAC_nnn'GTG
Cuts at: 3769 3769
Size: 7156
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6163 809
Size: 507 4847 1802
Fragments arranged by size:
4847 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2315 2414
Size: 54 1425 50 275 18 441 44 99
Cuts at: 2414 2490 2550 4435 4603 5492 7121 8
Size: 76 60 1885 168 889 1629 43
Fragments arranged by size:
1885 1629 1425 889 441 275 168 99
76 60 54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2271 2490 2550 4603 1537
Size: 275 459 219 60 2053 4090
Fragments arranged by size:
4090 2053 459 275 219 60
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2534 2981 3582 5086 1353
Size: 731 450 447 601 1504 3423
Fragments arranged by size:
3423 1504 731 601 450 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2627 5743 6117 6263 387
Size: 1064 138 1038 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 1038 374 146 138
EcoICRI GAG'CTC
Cuts at: 4187 4187
Size: 7156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2345 2873 2886 2907 3023 3446
Size: 393 437 276 528 13 21 116 423
Cuts at: 3446 3543 4041 4301 4361 5219 5816 6603 6871
Size: 97 498 260 60 858 597 787 268
Cuts at: 6871 1239
Size: 1524
Fragments arranged by size:
1524 858 787 597 528 498 437 423
393 276 268 260 116 97 60 21
13
EcoNI CCTnn'n_nnAGG
Cuts at: 4171 4171
Size: 7156
EcoO109I rG'GnC_Cy
Cuts at: 2074 2196 2207 2376 2429 2831 4429 2074
Size: 122 11 169 53 402 1598 4801
Fragments arranged by size:
4801 1598 402 169 122 53 11
EcoRI G'AATT_C
Cuts at: 4465 4843 4465
Size: 378 6778
EcoRV GAT'ATC
Cuts at: 2914 2914
Size: 7156
FseI GG_CCGG'CC
Cuts at: 2262 2262
Size: 7156
FspI TGC'GCA
Cuts at: 5662 5662
Size: 7156
HaeII r_GCGC'y
Cuts at: 1678 2312 2332 2408 2447 2511 6303 1678
Size: 634 20 76 39 64 3792 2531
Fragments arranged by size:
3792 2531 634 76 64 39 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2980 3012 3118 3150 3914
Size: 32 135 32 1408 32 106 32 764
Cuts at: 3914 3946 5797 5829 5871 5903 1373
Size: 32 1851 32 42 32 2626
Fragments arranged by size:
2626 1851 1408 764 135 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4500 4746 669
Size: 988 281 2562 246 3079
Fragments arranged by size:
3079 2562 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 748
Size: 2044 5112
HpaI GTT'AAC
Cuts at: 4746 4746
Size: 7156
KasI G'GCGC_C
Cuts at: 2507 2507
Size: 7156
KpnI G_GTAC'C
Cuts at: 4481 4481
Size: 7156
MfeI C'AATT_G
Cuts at: 4755 4755
Size: 7156
MluI A'CGCG_T
Cuts at: 7032 7032
Size: 7156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 3246 3664 6270 6454 1305
Size: 1704 237 418 2606 184 2007
Fragments arranged by size:
2606 2007 1704 418 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4437 10
Size: 54 1425 343 2605 2729
Fragments arranged by size:
2729 2605 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5114 5473 5632 518
Size: 648 3948 359 159 2042
Fragments arranged by size:
3948 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2253 2276 2286 2341 2467 3424 4572
Size: 573 74 23 10 55 126 957 1148
Cuts at: 4572 5249 6397 6642 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1148 957 677 573 245 126
74 55 23 10
NaeI GCC'GGC
Cuts at: 2260 4212 2260
Size: 1952 5204
NarI GG'CG_CC
Cuts at: 2508 2508
Size: 7156
NcoI C'CATG_G
Cuts at: 513 1065 2022 2661 513
Size: 552 957 639 5008
Fragments arranged by size:
5008 957 639 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7156
NgoMIV G'CCGG_C
Cuts at: 2258 4210 2258
Size: 1952 5204
NheI G'CTAG_C
Cuts at: 1052 3049 1052
Size: 1997 5159
NotI GC'GGCC_GC
Cuts at: 4603 4603
Size: 7156
NspI r_CATG'y
Cuts at: 1132 1229 4514 6059 1132
Size: 97 3285 1545 2229
Fragments arranged by size:
3285 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7156
PciI A'CATG_T
Cuts at: 6055 6055
Size: 7156
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 3424 1108
Size: 1605 711 4840
Fragments arranged by size:
4840 1605 711
PfoI T'CCnGG_A
Cuts at: 2067 2577 4141 2067
Size: 510 1564 5082
Fragments arranged by size:
5082 1564 510
PmeI GTTT'AAAC
Cuts at: 4460 4460
Size: 7156
PmlI CAC'GTG
Cuts at: 4115 4115
Size: 7156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2126 2158 5225 5257 6738 6770 2126
Size: 32 3067 32 1481 32 2512
Fragments arranged by size:
3067 2512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2429 2831 4429 2429
Size: 402 1598 5156
Fragments arranged by size:
5156 1598 402
PshAI GACnn'nnGTC
Cuts at: 1613 2829 1613
Size: 1216 5940
PsiI TTA'TAA
Cuts at: 3287 4726 3287
Size: 1439 5717
PspOMI G'GGCC_C
Cuts at: 2196 2207 2196
Size: 11 7145
PstI C_TGCA'G
Cuts at: 830 2451 3375 4508 830
Size: 1621 924 1133 3478
Fragments arranged by size:
3478 1621 1133 924
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7156
PvuII CAG'CTG
Cuts at: 2253 2467 3424 2253
Size: 214 957 5985
Fragments arranged by size:
5985 957 214
RsrII CG'GwC_CG
Cuts at: 2065 2065
Size: 7156
SacI G_AGCT'C
Cuts at: 4189 4189
Size: 7156
SacII CC_GC'GG
Cuts at: 2277 2287 2342 2277
Size: 10 55 7091
Fragments arranged by size:
7091 55 10
SalI G'TCGA_C
Cuts at: 1936 4498 1936
Size: 2562 4594
SanDI GG'GwC_CC
Cuts at: 2429 2429
Size: 7156
SbfI CC_TGCA'GG
Cuts at: 4508 4508
Size: 7156
ScaI AGT'ACT
Cuts at: 2744 5404 7116 2744
Size: 2660 1712 2784
Fragments arranged by size:
2784 2660 1712
SfcI C'TryA_G
Cuts at: 826 1045 2447 3371 4504 5639 6320 6511 826
Size: 219 1402 924 1133 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 1133 924 681 219 191
SfoI GGC'GCC
Cuts at: 2509 2509
Size: 7156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7156
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7156
SmaI CCC'GGG
Cuts at: 1872 4483 1872
Size: 2611 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3054 4020 4171 4352 5260 6161
Size: 197 926 1111 966 151 181 908 901
Cuts at: 6161 6423 820
Size: 262 1553
Fragments arranged by size:
1553 1111 966 926 908 901 262 197
181 151
SnaBI TAC'GTA
Cuts at: 493 4453 4947 493
Size: 3960 494 2702
Fragments arranged by size:
3960 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7156
SphI G_CATG'C
Cuts at: 1132 4514 1132
Size: 3382 3774
SspI AAT'ATT
Cuts at: 5 52 5080 5
Size: 47 5028 2081
Fragments arranged by size:
5028 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7156
StyI C'CwwG_G
Cuts at: 513 1065 2022 2661 2827 4122 4595 513
Size: 552 957 639 166 1295 473 3074
Fragments arranged by size:
3074 1295 957 639 552 473 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2047 5183 5200 5353 5538 5983 325
Size: 1255 467 3136 17 153 185 445 1498
Fragments arranged by size:
3136 1498 1255 467 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 5402 7114
Size: 274 80 33 51 1443 765 2660 1712
Cuts at: 7114 96
Size: 138
Fragments arranged by size:
2660 1712 1443 765 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3004 3036 7116 7148 3004
Size: 32 4080 32 3012
Fragments arranged by size:
4080 3012 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7156
XbaI T'CTAG_A
Cuts at: 4492 4492
Size: 7156
XhoI C'TCGA_G
Cuts at: 1943 3054 1943
Size: 1111 6045
XmaI C'CCGG_G
Cuts at: 1870 4481 1870
Size: 2611 4545
XmnI GAAnn'nnTTC
Cuts at: 3036 3135 5285 3036
Size: 99 2150 4907
Fragments arranged by size:
4907 2150 99
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4964 276
Size: 53 83 186 936 3430 2468
Fragments arranged by size:
3430 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BfrBI BsiWI BstZ17I FalI FspAI NruI NsiI
PacI PspXI PsrI SapI SexAI SfiI SrfI SwaI
XcmI