Restriction map for
pFN21ASDA1969
(Circular) (Six-base) MAPSORT of: pFN21ASDA1969.seq Check: 9555 from: 1 to: 6457
pFN21ASDA1969 6457 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3800 1937
Size: 1863 4594
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4520 5904 6172 1632
Size: 2888 1384 268 1917
Fragments arranged by size:
2888 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 5356 6333 1203
Size: 4153 977 1327
Fragments arranged by size:
4153 1327 977
AgeI A'CCGG_T
Cuts at: 2916 3336 5288 2916
Size: 420 1952 4085
Fragments arranged by size:
4085 1952 420
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2086 1184
Size: 902 5555
AlwNI CAG_nnn'CTG
Cuts at: 4200 5772 4200
Size: 1572 4885
ApaLI G'TGCA_C
Cuts at: 2489 4514 5670 2489
Size: 2025 1156 3276
Fragments arranged by size:
3276 2025 1156
ApoI r'AATT_y
Cuts at: 1493 2150 2194 2443 2582 2694 2710 2778 3198
Size: 657 44 249 139 112 16 68 420
Cuts at: 3198 3450 3618 3766 3992 4144 6253 1493
Size: 252 168 148 226 152 2109 1697
Fragments arranged by size:
2109 1697 657 420 252 249 226 168
152 148 139 112 68 44 16
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 3782 1870
Size: 73 1839 4545
Fragments arranged by size:
4545 1839 73
BamHI G'GATC_C
Cuts at: 3787 4154 3787
Size: 367 6090
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3778 5233 618
Size: 325 217 627 1991 1455 1842
Fragments arranged by size:
1991 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2301 3776 1391
Size: 910 1475 4072
Fragments arranged by size:
4072 1475 910
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 2997 928
Size: 971 331 767 4388
Fragments arranged by size:
4388 971 767 331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4648 4682 1903
Size: 34 2711 34 3678
Fragments arranged by size:
3678 2711 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2058 4349 5559 2058
Size: 2291 1210 2956
Fragments arranged by size:
2956 2291 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5068 136
Size: 107 122 71 631 605 3396 1525
Fragments arranged by size:
3396 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6452 6452
Size: 6457
BlpI GC'TnA_GC
Cuts at: 2187 3874 2187
Size: 1687 4770
Bme1580I G_kGCm'C
Cuts at: 1790 2493 4518 5674 1790
Size: 703 2025 1156 2573
Fragments arranged by size:
2573 2025 1156 703
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2264 2880 4188
Size: 416 53 83 399 24 847 616 1308
Cuts at: 4188 5146 442
Size: 958 1753
Fragments arranged by size:
1753 1308 958 847 616 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3735 3735
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2303 2808 2976 3060 3144 3228 3312
Size: 941 123 505 168 84 84 84 84
Cuts at: 3312 3396 3480 3730 5117 1239
Size: 84 84 250 1387 2579
Fragments arranged by size:
2579 1387 941 505 250 168 123 84
84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 4582 5447 5745 2039
Size: 2543 865 298 2751
Fragments arranged by size:
2751 2543 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5120 882
Size: 617 3621 2219
Fragments arranged by size:
3621 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3754 4248 493
Size: 711 2550 494 2702
Fragments arranged by size:
2702 2550 711 494
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4264 4646 5281 275
Size: 53 83 186 936 2731 382 635 1451
Fragments arranged by size:
2731 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2916 3336 4890 5288 5562 5709 6120 2916
Size: 420 1554 398 274 147 411 3253
Fragments arranged by size:
3253 1554 420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6232
Fragments arranged by size:
6232 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 1186
Size: 991 5466
BseYI C'CCAG_C
Cuts at: 2338 5660 6156 2338
Size: 3322 496 2639
Fragments arranged by size:
3322 2639 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3339 1163
Size: 573 1603 4281
Fragments arranged by size:
4281 1603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3907 4668 5696 1540
Size: 275 186 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 4518 4603 5674 2493
Size: 2025 85 1071 3276
Fragments arranged by size:
3276 2025 1071 85
BsmI GAATG_Cn'
Cuts at: 2177 3968 4061 2177
Size: 1791 93 4573
Fragments arranged by size:
4573 1791 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2493 3776 4518 4603 5674 1391
Size: 399 511 192 1283 742 85 1071 2174
Fragments arranged by size:
2174 1283 1071 742 511 399 192 85
BspEI T'CCGG_A
Cuts at: 6120 6120
Size: 6457
BspHI T'CATG_A
Cuts at: 4346 6076 4346
Size: 1730 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3812 844
Size: 2968 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4344 1394
Size: 429 2521 3507
Fragments arranged by size:
3507 2521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4952 5126 65
Size: 1183 3704 174 1396
Fragments arranged by size:
3704 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2916 3336 5101 5288 1700
Size: 110 1106 420 1765 187 2869
Fragments arranged by size:
2869 1765 1106 420 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6457
BstBI TT'CG_AA
Cuts at: 2007 5261 2007
Size: 3254 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6457
BstYI r'GATC_y
Cuts at: 1972 3787 4154 4539 4556 6008 6094 6106 6452
Size: 1815 367 385 17 1452 86 12 346
Cuts at: 6452 1972
Size: 1977
Fragments arranged by size:
1977 1815 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4948
Fragments arranged by size:
4948 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5718
Fragments arranged by size:
5718 683 56
BtsI GCAGTG_nn'
Cuts at: 3973 4767 4787 3973
Size: 794 20 5643
Fragments arranged by size:
5643 794 20
ClaI AT'CG_AT
Cuts at: 1554 3085 1554
Size: 1531 4926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6422
DraI TTT'AAA
Cuts at: 2589 2746 3761 2589
Size: 157 1015 5285
Fragments arranged by size:
5285 1015 157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5464 809
Size: 507 4148 1802
Fragments arranged by size:
4148 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2372 3904
Size: 54 1425 50 275 18 312 230 1532
Cuts at: 3904 4793 6422 8
Size: 889 1629 43
Fragments arranged by size:
1629 1532 1425 889 312 275 230 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3904 1537
Size: 275 2092 4090
Fragments arranged by size:
4090 2092 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 4387 1353
Size: 953 2081 3423
Fragments arranged by size:
3423 2081 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5044 5418 5564 387
Size: 1064 138 3455 374 146 1280
Fragments arranged by size:
3455 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2303 2808 2976 3060 3144 3228
Size: 393 548 123 505 168 84 84 84
Cuts at: 3228 3312 3396 3480 3730 4520 5117 5904 6172
Size: 84 84 84 250 790 597 787 268
Cuts at: 6172 1239
Size: 1524
Fragments arranged by size:
1524 790 787 597 548 505 393 268
250 168 123 84 84 84 84 84
84
EcoRI G'AATT_C
Cuts at: 2150 2694 2710 2778 3198 3450 3766 4144 2150
Size: 544 16 68 420 252 316 378 4463
Fragments arranged by size:
4463 544 420 378 316 252 68 16
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 5604 1678
Size: 3926 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5098 5130 5172 5204 1373
Size: 32 135 32 3526 32 42 32 2626
Fragments arranged by size:
3526 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2684 3801 4047 669
Size: 988 281 746 1117 246 3079
Fragments arranged by size:
3079 1117 988 746 281 246
HindIII A'AGCT_T
Cuts at: 748 2739 2823 2991 3075 3327 3411 3495 748
Size: 1991 84 168 84 252 84 84 3710
Fragments arranged by size:
3710 1991 252 168 84 84 84 84
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5571 5755 1305
Size: 4266 184 2007
Fragments arranged by size:
4266 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2374 10
Size: 54 1425 343 312 230 4093
Fragments arranged by size:
4093 1425 343 312 230 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2887 4415 4774 4933 518
Size: 648 1721 1528 359 159 2042
Fragments arranged by size:
2042 1721 1528 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3873 4550 5698 5943 1606
Size: 2267 677 1148 245 2120
Fragments arranged by size:
2267 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4948
Fragments arranged by size:
4948 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 2668 3815 5360 1132
Size: 97 1439 1147 1545 2229
Fragments arranged by size:
2229 1545 1439 1147 97
PasI CC'CwG_GG
Cuts at: 1348 2075 1348
Size: 727 5730
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6457
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6457
PsiI TTA'TAA
Cuts at: 2505 4027 2505
Size: 1522 4935
PstI C_TGCA'G
Cuts at: 830 3809 830
Size: 2979 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SexAI A'CCwGG_T
Cuts at: 2232 2232
Size: 6457
SfcI C'TryA_G
Cuts at: 826 1045 3805 4940 5621 5812 826
Size: 219 2760 1135 681 191 1471
Fragments arranged by size:
2760 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 3784 1872
Size: 1912 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 4561 5462 5724 820
Size: 197 926 111 2507 901 262 1553
Fragments arranged by size:
2507 1553 926 901 262 197 111
SnaBI TAC'GTA
Cuts at: 493 3754 4248 493
Size: 3261 494 2702
Fragments arranged by size:
3261 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 2668 3815 1132
Size: 1536 1147 3774
Fragments arranged by size:
3774 1536 1147
SspI AAT'ATT
Cuts at: 5 52 2547 4381 5
Size: 47 2495 1834 2081
Fragments arranged by size:
2495 2081 1834 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6457
StyI C'CwwG_G
Cuts at: 513 1065 2022 3896 513
Size: 552 957 1874 3074
Fragments arranged by size:
3074 1874 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 4484 4501 4654 4839 5284 325
Size: 1255 654 2250 17 153 185 445 1498
Fragments arranged by size:
2250 1498 1255 654 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3017 4703 6415
Size: 274 80 33 51 1443 1040 1686 1712
Cuts at: 6415 96
Size: 138
Fragments arranged by size:
1712 1686 1443 1040 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6417 6449 6417
Size: 32 6425
Tth111I GACn'n_nGTC
Cuts at: 1316 2270 1316
Size: 954 5503
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6457
XmaI C'CCGG_G
Cuts at: 1870 3782 1870
Size: 1912 4545
XmnI GAAnn'nnTTC
Cuts at: 2630 2798 4586 2630
Size: 168 1788 4501
Fragments arranged by size:
4501 1788 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI