Restriction map for
pFN21ASDA1963
(Circular) (Six-base) MAPSORT of: pFN21ASDA1963.seq Check: 2108 from: 1 to: 5698
pFN21ASDA1963 5698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2448 2448
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2991 3041 1937
Size: 1054 50 4594
Fragments arranged by size:
4594 1054 50
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2331 4597 5574 1203
Size: 1128 2266 977 1327
Fragments arranged by size:
2266 1327 1128 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2129 2456 2914 1184
Size: 945 327 458 3968
Fragments arranged by size:
3968 945 458 327
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2474 3023 1870
Size: 73 531 549 4545
Fragments arranged by size:
4545 549 531 73
BamHI G'GATC_C
Cuts at: 2067 2361 2819 3028 3395 2067
Size: 294 458 209 367 4370
Fragments arranged by size:
4370 458 367 294 209
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2532 3019 4474 618
Size: 325 217 627 381 364 487 1455 1842
Fragments arranged by size:
1842 1455 627 487 381 364 325 217
BanII G_rGCy'C
Cuts at: 1391 2604 3017 1391
Size: 1213 413 4072
Fragments arranged by size:
4072 1213 413
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 5698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2765 928
Size: 971 866 3861
Fragments arranged by size:
3861 971 866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2166 4309 136
Size: 107 122 71 631 605 494 2143 1525
Fragments arranged by size:
2143 1525 631 605 494 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2451 3759 4915 1790
Size: 328 333 1308 1156 2573
Fragments arranged by size:
2573 1308 1156 333 328
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 4921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 2743 4358 1239
Size: 1379 125 1615 2579
Fragments arranged by size:
2579 1615 1379 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2638 4361 882
Size: 617 1139 1723 2219
Fragments arranged by size:
2219 1723 1139 617
BsaAI yAC'GTr
Cuts at: 493 1204 2446 2995 3489 493
Size: 711 1242 549 494 2702
Fragments arranged by size:
2702 1242 711 549 494
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 3505 3887 4522
Size: 53 83 186 936 636 1336 382 635
Cuts at: 4522 275
Size: 1451
Fragments arranged by size:
1451 1336 936 636 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2166 2196 1178
Size: 30 165 30 763 30 4680
Fragments arranged by size:
4680 763 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5698
BseYI C'CCAG_C
Cuts at: 2429 2823 4901 5397 2429
Size: 394 2078 496 2730
Fragments arranged by size:
2730 2078 496 394
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2339 2426 2884 1163
Size: 573 585 18 87 458 3977
Fragments arranged by size:
3977 585 573 458 87 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2234 3148 3909 4937 1540
Size: 275 186 233 914 761 1028 2301
Fragments arranged by size:
2301 1028 914 761 275 233 186
BsiHKAI G_wGCw'C
Cuts at: 2565 2604 3759 3844 4915 2565
Size: 39 1155 85 1071 3348
Fragments arranged by size:
3348 1155 1071 85 39
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2451 2565 2604 3017 3759 3844
Size: 399 328 333 114 39 413 742 85
Cuts at: 3844 4915 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 413 399 333 328 114
85 39
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 3053 844
Size: 1604 605 3489
Fragments arranged by size:
3489 1604 605
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 2182 3585 1394
Size: 429 299 60 1403 3507
Fragments arranged by size:
3507 1403 429 299 60
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2717 4342 4529 1700
Size: 110 907 1625 187 2869
Fragments arranged by size:
2869 1625 907 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 2384 2485 2545 2547 1925
Size: 459 101 60 2 5076
Fragments arranged by size:
5076 459 101 60 2
BssSI C'ACGA_G
Cuts at: 2912 3758 4770 2912
Size: 846 1012 3840
Fragments arranged by size:
3840 1012 846
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2762 794
Size: 1968 3730
BstBI TT'CG_AA
Cuts at: 2007 4502 2007
Size: 2495 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 2752
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2067 2361 2819 3028 3395 3780 3797 5249
Size: 95 294 458 209 367 385 17 1452
Cuts at: 5249 5335 5347 5693 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 458 385 367 346 294 209
95 86 17 12
BstZ17I GTA'TAC
Cuts at: 2992 2992
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 2161 2456 513
Size: 552 1096 295 3755
Fragments arranged by size:
3755 1096 552 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2231 2325 3145
Size: 54 1425 50 275 18 401 94 820
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 820 401 275 94 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2231 3145 1537
Size: 275 419 914 4090
Fragments arranged by size:
4090 914 419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2570 2609 3628 1353
Size: 1217 39 1019 3423
Fragments arranged by size:
3423 1217 1019 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2327 4285 4659 4805 387
Size: 1064 138 738 1958 374 146 1280
Fragments arranged by size:
1958 1280 1064 738 374 146 138
EcoICRI GAG'CTC
Cuts at: 2602 2602
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 2743 3761 4358 5145 5413 1239
Size: 393 986 125 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 986 787 597 393 268 125
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2369
Size: 5698
EcoO109I rG'GnC_Cy
Cuts at: 2208 2208
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2172 2464 2671 4845 1678
Size: 494 292 207 2174 2531
Fragments arranged by size:
2531 2174 494 292 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 4339 4371 4413
Size: 32 135 32 594 32 2141 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 2141 594 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 10
Size: 54 1425 343 495 3381
Fragments arranged by size:
3381 1425 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2750 3656 4015 4174 518
Size: 648 918 666 906 359 159 2042
Fragments arranged by size:
2042 918 906 666 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2163 2257 2353 2458 2683 3114 3791 4939
Size: 557 94 96 105 225 431 677 1148
Cuts at: 4939 5184 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 557 431 245 225 105
96 94
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PfoI T'CCnGG_A
Cuts at: 2607 2607
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PmlI CAC'GTG
Cuts at: 2446 2446
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2208
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2257 2353 2257
Size: 96 5602
SacI G_AGCT'C
Cuts at: 2604 2604
Size: 5698
SacII CC_GC'GG
Cuts at: 2164 2459 2164
Size: 295 5403
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SanDI GG'GwC_CC
Cuts at: 2208 2208
Size: 5698
SapI GCTCTTCn'nnn_
Cuts at: 2570 2609 2570
Size: 39 5659
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2287 2590 3046 4181 4862 5053 826
Size: 219 1242 303 456 1135 681 191 1471
Fragments arranged by size:
1471 1242 1135 681 456 303 219 191
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 2476 3025 1872
Size: 604 549 4545
Fragments arranged by size:
4545 604 549
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2995 3489 493
Size: 2502 494 2702
Fragments arranged by size:
2702 2502 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SrfI GCCC'GGGC
Cuts at: 2476 2476
Size: 5698
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 2737 1517
Size: 1220 4478
StyI C'CwwG_G
Cuts at: 513 1065 3137 513
Size: 552 2072 3074
Fragments arranged by size:
3074 2072 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 2474 3023 1870
Size: 604 549 4545
Fragments arranged by size:
4545 604 549
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SexAI SfiI SwaI XcmI