Restriction map for
pFN21ASDA1951
(Circular) (Six-base) MAPSORT of: pFN21ASDA1951.seq Check: 6379 from: 1 to: 6739
pFN21ASDA1951 6739 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3346 3501 3346
Size: 155 6584
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4549 278
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3111 4082 1937
Size: 1174 971 4594
Fragments arranged by size:
4594 1174 971
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4802 6186 6454 1632
Size: 3170 1384 268 1917
Fragments arranged by size:
3170 1917 1384 268
AfeI AGC'GCT
Cuts at: 3629 3629
Size: 6739
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 5638 6615 1203
Size: 4435 977 1327
Fragments arranged by size:
4435 1327 977
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 3019 3146 3405 3441 3507 3534 1166
Size: 1853 127 259 36 66 27 4371
Fragments arranged by size:
4371 1853 259 127 66 36 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2280 2532 3423 3509 1184
Size: 1096 252 891 86 4414
Fragments arranged by size:
4414 1096 891 252 86
AlwNI CAG_nnn'CTG
Cuts at: 2464 3943 4482 6054 2464
Size: 1479 539 1572 3149
Fragments arranged by size:
3149 1572 1479 539
ApaI G_GGCC'C
Cuts at: 2343 3001 3166 3423 2343
Size: 658 165 257 5659
Fragments arranged by size:
5659 658 257 165
ApaLI G'TGCA_C
Cuts at: 3594 3895 4796 5952 3594
Size: 301 901 1156 4381
Fragments arranged by size:
4381 1156 901 301
ApoI r'AATT_y
Cuts at: 1493 4048 4274 4426 6535 1493
Size: 2555 226 152 2109 1697
Fragments arranged by size:
2555 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 3810 4064 1870
Size: 73 534 1333 254 4545
Fragments arranged by size:
4545 1333 534 254 73
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 2672 3265 3399 4060
Size: 325 217 627 536 349 593 134 661
Cuts at: 4060 5515 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 661 627 593 536 349 325
217 134
BanII G_rGCy'C
Cuts at: 1391 2343 2391 2610 2839 2884 3001 3166 3309
Size: 952 48 219 229 45 117 165 143
Cuts at: 3309 3423 3644 3817 4008 4058 1391
Size: 114 221 173 191 50 4072
Fragments arranged by size:
4072 952 229 221 219 191 173 165
143 117 114 50 48 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3034 928
Size: 971 1135 4633
Fragments arranged by size:
4633 1135 971
BbvCI CC'TCA_GC
Cuts at: 2040 2334 2872 3379 3566 2040
Size: 294 538 507 187 5213
Fragments arranged by size:
5213 538 507 294 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3173 3207 4930 4964 1903
Size: 34 1236 34 1723 34 3678
Fragments arranged by size:
3678 1723 1236 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6739
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5350 136
Size: 107 122 71 631 605 3678 1525
Fragments arranged by size:
3678 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6734 6734
Size: 6739
BlpI GC'TnA_GC
Cuts at: 2529 4156 2529
Size: 1627 5112
Bme1580I G_kGCm'C
Cuts at: 1790 2343 2675 3001 3166 3268 3423 3598 3899
Size: 553 332 326 165 102 155 175 301
Cuts at: 3899 4800 5956 1790
Size: 901 1156 2573
Fragments arranged by size:
2573 1156 901 553 332 326 301 175
165 155 102
BmgBI CAC'GTC
Cuts at: 1318 3599 1318
Size: 2281 4458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3992 4470 5428
Size: 416 53 83 399 24 2575 478 958
Cuts at: 5428 442
Size: 1753
Fragments arranged by size:
2575 1753 958 478 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2330 3282 3855 5399 1239
Size: 1010 81 952 573 1544 2579
Fragments arranged by size:
2579 1544 1010 952 573 81
Bpu10I CC'TnA_GC
Cuts at: 2040 2334 2838 2872 3379 3566 2040
Size: 294 504 34 507 187 5213
Fragments arranged by size:
5213 507 504 294 187 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3083 3971 4864 5729 6027 3083
Size: 888 893 865 298 3795
Fragments arranged by size:
3795 893 888 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3725 3905 5402 882
Size: 617 2226 180 1497 2219
Fragments arranged by size:
2226 2219 1497 617 180
BsaAI yAC'GTr
Cuts at: 493 1204 3481 4036 4530 493
Size: 711 2277 555 494 2702
Fragments arranged by size:
2702 2277 711 555 494
BsaBI GATnn'nnATC
Cuts at: 3847 6733 3847
Size: 2886 3853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 4546 4928 5563
Size: 53 83 186 936 1900 1113 382 635
Cuts at: 5563 275
Size: 1451
Fragments arranged by size:
1900 1451 1113 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 1178
Size: 30 165 30 1266 30 5218
Fragments arranged by size:
5218 1266 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2294 2666 2714 2732 2735 2753 2789
Size: 1105 3 372 48 18 3 18 36
Cuts at: 2789 1186
Size: 5136
Fragments arranged by size:
5136 1105 372 48 36 18 18 3
3
BseYI C'CCAG_C
Cuts at: 2448 2505 2832 2859 3292 3342 3904 3936 5942
Size: 57 327 27 433 50 562 32 2006
Cuts at: 5942 6438 2448
Size: 496 2749
Fragments arranged by size:
2749 2006 562 496 433 327 57 50
32 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3429 3963 1163
Size: 573 1693 534 3939
Fragments arranged by size:
3939 1693 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2201 2391 2630 3365 3598 3644 3817 3899 4800
Size: 190 239 735 233 46 173 82 901
Cuts at: 4800 4885 5956 2201
Size: 85 1071 2984
Fragments arranged by size:
2984 1071 901 735 239 233 190 173
85 82 46
BsmI GAATG_Cn'
Cuts at: 2363 4250 4343 2363
Size: 1887 93 4759
Fragments arranged by size:
4759 1887 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2343 2391 2610 2630 2675 2839
Size: 399 411 142 48 219 20 45 164
Cuts at: 2839 2884 3001 3166 3268 3309 3365 3423 3598
Size: 45 117 165 102 41 56 58 175
Cuts at: 3598 3644 3817 3899 4008 4058 4800 4885 5956
Size: 46 173 82 109 50 742 85 1071
Cuts at: 5956 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 411 399 219 175 173
165 164 142 117 109 102 85 82
58 56 50 48 46 45 45 41
20
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 4628 6358 4628
Size: 1730 5009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 3346 3501 3657 4094 844
Size: 2194 308 155 156 437 3489
Fragments arranged by size:
3489 2194 437 308 156 155
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2713 3179 5234 5408 65
Size: 1183 1465 466 2055 174 1396
Fragments arranged by size:
2055 1465 1396 1183 466 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2886 2895 2976 3156 3237 3251 3402
Size: 110 1076 9 81 180 81 14 151
Cuts at: 3402 3660 5383 5570 1700
Size: 258 1723 187 2869
Fragments arranged by size:
2869 1723 1076 258 187 180 151 110
81 81 14 9
BsrGI T'GTAC_A
Cuts at: 96 3023 96
Size: 2927 3812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2260 3073 794
Size: 1466 813 4460
Fragments arranged by size:
4460 1466 813
BstBI TT'CG_AA
Cuts at: 2007 5543 2007
Size: 3536 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 3008 3536 3008
Size: 528 6211
BstYI r'GATC_y
Cuts at: 1972 2433 4069 4436 4821 4838 6290 6376 6388
Size: 461 1636 367 385 17 1452 86 12
Cuts at: 6388 6734 1972
Size: 346 1977
Fragments arranged by size:
1977 1636 1452 461 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2649 2649
Size: 6739
BtgI C'CryG_G
Cuts at: 513 1065 2022 2781 3121 3774 513
Size: 552 957 759 340 653 3478
Fragments arranged by size:
3478 957 759 653 552 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3042 3068 3170 3901 487
Size: 683 56 1816 26 102 731 3325
Fragments arranged by size:
3325 1816 731 683 102 56 26
BtsI GCAGTG_nn'
Cuts at: 2085 2567 2938 3168 4255 5049 5069 2085
Size: 482 371 230 1087 794 20 3755
Fragments arranged by size:
3755 1087 794 482 371 230 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2532 2567 529
Size: 35 1968 35 4701
Fragments arranged by size:
4701 1968 35 35
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3118 3558
Size: 54 1425 50 275 18 216 1072 440
Cuts at: 3558 3677 3757 4186 5075 6704 8
Size: 119 80 429 889 1629 43
Fragments arranged by size:
1629 1425 1072 889 440 429 275 216
119 80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2721 2754 4669 1353
Size: 824 544 33 1915 3423
Fragments arranged by size:
3423 1915 824 544 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2914 2959 5326 5700 5846 387
Size: 1064 138 1325 45 2367 374 146 1280
Fragments arranged by size:
2367 1325 1280 1064 374 146 138 45
EcoICRI GAG'CTC
Cuts at: 2389 3642 3815 2389
Size: 1253 173 5313
Fragments arranged by size:
5313 1253 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2330 3282 3855 4802 5399 6186
Size: 393 617 81 952 573 947 597 787
Cuts at: 6186 6454 1239
Size: 268 1524
Fragments arranged by size:
1524 952 947 787 617 597 573 393
268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3273 3273
Size: 6739
EcoO109I rG'GnC_Cy
Cuts at: 2155 2339 2340 3420 3516 2155
Size: 184 1 1080 96 5378
Fragments arranged by size:
5378 1080 184 96 1
EcoRI G'AATT_C
Cuts at: 4048 4426 4048
Size: 378 6361
FspI TGC'GCA
Cuts at: 3485 5245 3485
Size: 1760 4979
HaeII r_GCGC'y
Cuts at: 1678 3631 3950 5886 1678
Size: 1953 319 1936 2531
Fragments arranged by size:
2531 1953 1936 319
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5380 5412 5454 5486 1373
Size: 32 135 32 3808 32 42 32 2626
Fragments arranged by size:
3808 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2091 2124 4083 4329 669
Size: 988 281 153 33 1959 246 3079
Fragments arranged by size:
3079 1959 988 281 246 153 33
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 3669 3996 5853 6037 1305
Size: 1389 975 327 1857 184 2007
Fragments arranged by size:
2007 1857 1389 975 327 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3560 10
Size: 54 1425 343 1728 3189
Fragments arranged by size:
3189 1728 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2403 3006 3019 3146 3405 3441 3507
Size: 648 1237 603 13 127 259 36 66
Cuts at: 3507 3534 3591 4697 5056 5215 518
Size: 27 57 1106 359 159 2042
Fragments arranged by size:
2042 1237 1106 648 603 359 259 159
127 66 57 36 27 13
MspA1I CmG'CkG
Cuts at: 1606 2828 3123 3302 3346 3441 3580 3762 3799
Size: 1222 295 179 44 95 139 182 37
Cuts at: 3799 3967 4155 4832 5980 6225 1606
Size: 168 188 677 1148 245 2120
Fragments arranged by size:
2120 1222 1148 677 295 245 188 182
179 168 139 95 44 37
NaeI GCC'GGC
Cuts at: 2888 3253 2888
Size: 365 6374
NcoI C'CATG_G
Cuts at: 513 1065 2022 2781 3774 513
Size: 552 957 759 993 3478
Fragments arranged by size:
3478 993 957 759 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NgoMIV G'CCGG_C
Cuts at: 2886 3251 2886
Size: 365 6374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 2263 4097 5642 1132
Size: 97 1034 1834 1545 2229
Fragments arranged by size:
2229 1834 1545 1034 97
PasI CC'CwG_GG
Cuts at: 1348 2381 1348
Size: 1033 5706
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3613 3943 1108
Size: 2505 330 3904
Fragments arranged by size:
3904 2505 330
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PmlI CAC'GTG
Cuts at: 3481 3481
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 4309 4309
Size: 6739
PspOMI G'GGCC_C
Cuts at: 2339 2997 3162 3419 2339
Size: 658 165 257 5659
Fragments arranged by size:
5659 658 257 165
PstI C_TGCA'G
Cuts at: 830 2945 2985 3654 3966 4091 830
Size: 2115 40 669 312 125 3478
Fragments arranged by size:
3478 2115 669 312 125 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2828 3346 3580 3967 2828
Size: 518 234 387 5600
Fragments arranged by size:
5600 518 387 234
SacI G_AGCT'C
Cuts at: 2391 3644 3817 2391
Size: 1253 173 5313
Fragments arranged by size:
5313 1253 173
SacII CC_GC'GG
Cuts at: 3124 3124
Size: 6739
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 4987 6699 4987
Size: 1712 5027
SfcI C'TryA_G
Cuts at: 826 1045 2941 2981 3650 3830 3962 4087 5222
Size: 219 1896 40 669 180 132 125 1135
Cuts at: 5222 5903 6094 826
Size: 681 191 1471
Fragments arranged by size:
1896 1471 1135 681 669 219 191 180
132 125 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 2895 3156 3237 3402 3660 1700
Size: 1195 261 81 165 258 4779
Fragments arranged by size:
4779 1195 261 258 165 81
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3062 3950 4843 5744 6006 820
Size: 197 926 1119 888 893 901 262 1553
Fragments arranged by size:
1553 1119 926 901 893 888 262 197
SnaBI TAC'GTA
Cuts at: 493 4036 4530 493
Size: 3543 494 2702
Fragments arranged by size:
3543 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 4663 5
Size: 47 4611 2081
Fragments arranged by size:
4611 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2022 2781 3036 3774 4178 513
Size: 552 957 759 255 738 404 3074
Fragments arranged by size:
3074 957 759 738 552 404 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2476 2690 2942 4766 4783 4936 5121
Size: 1255 896 214 252 1824 17 153 185
Cuts at: 5121 5566 325
Size: 445 1498
Fragments arranged by size:
1824 1498 1255 896 445 252 214 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3023 3412 4985
Size: 274 80 33 51 1443 1046 389 1573
Cuts at: 4985 6697 96
Size: 1712 138
Fragments arranged by size:
1712 1573 1443 1046 389 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 6699 6731 2669
Size: 32 3998 32 2677
Fragments arranged by size:
3998 2677 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4547 276
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PpuMI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI