Restriction map for
pFN21ASDA1950
(Circular) (Six-base) MAPSORT of: pFN21ASDA1950.seq Check: 3436 from: 1 to: 6223
pFN21ASDA1950 6223 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3298 3298
Size: 6223
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2035 3566 1937
Size: 98 1531 4594
Fragments arranged by size:
4594 1531 98
Acc65I G'GTAC_C
Cuts at: 2964 3544 2964
Size: 580 5643
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2584 2746 4286 5670 5938 1632
Size: 952 162 1540 1384 268 1917
Fragments arranged by size:
1917 1540 1384 952 268 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 5122 6099 1203
Size: 3919 977 1327
Fragments arranged by size:
3919 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 3110 1166
Size: 1944 4279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6223
AlwNI CAG_nnn'CTG
Cuts at: 2113 2748 3274 3966 5538 2113
Size: 635 526 692 1572 2798
Fragments arranged by size:
2798 1572 692 635 526
ApaI G_GGCC'C
Cuts at: 2064 2679 2755 3221 2064
Size: 615 76 466 5066
Fragments arranged by size:
5066 615 466 76
ApaLI G'TGCA_C
Cuts at: 4280 5436 4280
Size: 1156 5067
ApoI r'AATT_y
Cuts at: 1493 2660 3532 3758 3910 6019 1493
Size: 1167 872 226 152 2109 1697
Fragments arranged by size:
2109 1697 1167 872 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2234 2243 2723 3070 3548 1870
Size: 73 121 170 9 480 347 478 4545
Fragments arranged by size:
4545 480 478 347 170 121 73 9
BamHI G'GATC_C
Cuts at: 3553 3920 3553
Size: 367 5856
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2964 3104 3544 4999 618
Size: 325 217 627 1177 140 440 1455 1842
Fragments arranged by size:
1842 1455 1177 627 440 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2064 2679 2755 2811 3102 3123 3221 3542
Size: 673 615 76 56 291 21 98 321
Cuts at: 3542 1391
Size: 4072
Fragments arranged by size:
4072 673 615 321 291 98 76 56
21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2194 2228 4414 4448 1903
Size: 34 257 34 2186 34 3678
Fragments arranged by size:
3678 2186 257 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3197 4834 136
Size: 107 122 71 631 605 1525 1637 1525
Fragments arranged by size:
1637 1525 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6218 6218
Size: 6223
BlpI GC'TnA_GC
Cuts at: 2870 3176 3640 2870
Size: 306 464 5453
Fragments arranged by size:
5453 464 306
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2622 2679 2755 2770 3221 3463 4284
Size: 274 558 57 76 15 451 242 821
Cuts at: 4284 5440 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 821 558 451 274 242 76
57 15
BmgBI CAC'GTC
Cuts at: 1318 3006 1318
Size: 1688 4535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3954 4912 442
Size: 416 53 83 399 24 2537 958 1753
Fragments arranged by size:
2537 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2608 4883 1239
Size: 1226 143 2275 2579
Fragments arranged by size:
2579 2275 1226 143
Bpu10I CC'TnA_GC
Cuts at: 2225 2330 2225
Size: 105 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4348 5213 5511 4348
Size: 865 298 5060
Fragments arranged by size:
5060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2092 4886 882
Size: 617 593 2794 2219
Fragments arranged by size:
2794 2219 617 593
BsaAI yAC'GTr
Cuts at: 493 1204 2799 3520 4014 493
Size: 711 1595 721 494 2702
Fragments arranged by size:
2702 1595 721 711 494
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2631 3327 4030 4412
Size: 53 83 186 936 1098 696 703 382
Cuts at: 4412 5047 275
Size: 635 1451
Fragments arranged by size:
1451 1098 936 703 696 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2280 4656 5054 5328 5475 5886 2280
Size: 2376 398 274 147 411 2617
Fragments arranged by size:
2617 2376 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3419 3449 1178
Size: 30 165 30 2016 30 3952
Fragments arranged by size:
3952 2016 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2440 3308 3427 3460 1186
Size: 1165 89 868 119 33 3949
Fragments arranged by size:
3949 1165 868 119 89 33
BseYI C'CCAG_C
Cuts at: 2459 2548 2678 2748 3011 3339 3386 3421 5426
Size: 89 130 70 263 328 47 35 2005
Cuts at: 5426 5922 2459
Size: 496 2760
Fragments arranged by size:
2760 2005 496 328 263 130 89 70
47 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2173 2345 2362 2392 3326 1163
Size: 573 437 172 17 30 934 4060
Fragments arranged by size:
4060 934 573 437 172 30 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 3673 4434 5462 1540
Size: 275 186 1336 336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 336 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 4284 4369 5440 2811
Size: 1473 85 1071 3594
Fragments arranged by size:
3594 1473 1071 85
BsmI GAATG_Cn'
Cuts at: 3734 3827 3734
Size: 93 6130
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2622 2679 2755 2770 2811 3102
Size: 399 274 558 57 76 15 41 291
Cuts at: 3102 3123 3221 3463 3542 4284 4369 5440 1391
Size: 21 98 242 79 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 558 399 291 274 242
98 85 79 76 57 41 21 15
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 4112 5842 4112
Size: 1730 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3298 3578 844
Size: 2454 280 3489
Fragments arranged by size:
3489 2454 280
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 4110 1394
Size: 429 320 1967 3507
Fragments arranged by size:
3507 1967 429 320
BsrDI GCAATG_nn'
Cuts at: 65 1248 4718 4892 65
Size: 1183 3470 174 1396
Fragments arranged by size:
3470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2341 4867 5054 1700
Size: 110 531 2526 187 2869
Fragments arranged by size:
2869 2526 531 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 2804 4283 5295 2804
Size: 1479 1012 3732
Fragments arranged by size:
3732 1479 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6223
BstBI TT'CG_AA
Cuts at: 2007 5027 2007
Size: 3020 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6223
BstYI r'GATC_y
Cuts at: 1972 3553 3920 4305 4322 5774 5860 5872 6218
Size: 1581 367 385 17 1452 86 12 346
Cuts at: 6218 1972
Size: 1977
Fragments arranged by size:
1977 1581 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2921 3157 3443 2921
Size: 236 286 5701
Fragments arranged by size:
5701 286 236
BtgI C'CryG_G
Cuts at: 513 1065 3321 513
Size: 552 2256 3415
Fragments arranged by size:
3415 2256 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 3739 4533 4553 2036
Size: 1703 794 20 3706
Fragments arranged by size:
3706 1703 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DraIII CAC_nnn'GTG
Cuts at: 2626 2626
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2084 5230 809
Size: 507 768 3146 1802
Fragments arranged by size:
3146 1802 768 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 3255 3366
Size: 54 1425 50 275 18 324 1101 111
Cuts at: 3366 3670 4559 6188 8
Size: 304 889 1629 43
Fragments arranged by size:
1629 1425 1101 889 324 304 275 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2189 3504 4153 1353
Size: 800 36 1315 649 3423
Fragments arranged by size:
3423 1315 800 649 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2113 4810 5184 5330 387
Size: 1064 138 524 2697 374 146 1280
Fragments arranged by size:
2697 1280 1064 524 374 146 138
EcoICRI GAG'CTC
Cuts at: 2809 2809
Size: 6223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2584 2608 2746 4286 4883 5670
Size: 393 833 119 24 138 1540 597 787
Cuts at: 5670 5938 1239
Size: 268 1524
Fragments arranged by size:
1540 1524 833 787 597 393 268 138
119 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2156 2333 2702 2858 2879 3305 3442 2156
Size: 177 369 156 21 426 137 4937
Fragments arranged by size:
4937 426 369 177 156 137 21
EcoO109I rG'GnC_Cy
Cuts at: 2061 2675 2728 2752 2884 3217 3218 3271 3298
Size: 614 53 24 132 333 1 53 27
Cuts at: 3298 3379 2061
Size: 81 4905
Fragments arranged by size:
4905 614 333 132 81 53 53 27
24 1
EcoRI G'AATT_C
Cuts at: 2660 3532 3910 2660
Size: 872 378 4973
Fragments arranged by size:
4973 872 378
FspI TGC'GCA
Cuts at: 3413 4729 3413
Size: 1316 4907
HaeII r_GCGC'y
Cuts at: 1678 2685 3066 5370 1678
Size: 1007 381 2304 2531
Fragments arranged by size:
2531 2304 1007 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3419 3451 4864 4896 4938
Size: 32 135 32 1847 32 1413 32 42
Cuts at: 4938 4970 1373
Size: 32 2626
Fragments arranged by size:
2626 1847 1413 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3567 3813 669
Size: 988 281 1629 246 3079
Fragments arranged by size:
3079 1629 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6223
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KpnI G_GTAC'C
Cuts at: 2968 3548 2968
Size: 580 5643
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3096 5337 5521 1305
Size: 1791 2241 184 2007
Fragments arranged by size:
2241 2007 1791 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3257 10
Size: 54 1425 343 1425 2976
Fragments arranged by size:
2976 1425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3110 4181 4540 4699 518
Size: 648 1944 1071 359 159 2042
Fragments arranged by size:
2042 1944 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2368 2376 2445 2841 2850 3205 3280
Size: 507 255 8 69 396 9 355 75
Cuts at: 3280 3339 3639 4316 5464 5709 1606
Size: 59 300 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 507 396 355 300 255
245 75 69 59 9 8
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5671
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 2939 3079 3581 5126 1132
Size: 97 1710 140 502 1545 2229
Fragments arranged by size:
2229 1710 1545 502 140 97
PasI CC'CwG_GG
Cuts at: 1348 2770 3498 1348
Size: 1422 728 4073
Fragments arranged by size:
4073 1422 728
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2821 1108
Size: 1713 4510
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PmlI CAC'GTG
Cuts at: 2799 2799
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3298 3379 3298
Size: 81 6142
PshAI GACnn'nnGTC
Cuts at: 1613 2907 1613
Size: 1294 4929
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PspOMI G'GGCC_C
Cuts at: 2060 2675 2751 3217 2060
Size: 615 76 466 5066
Fragments arranged by size:
5066 615 466 76
PstI C_TGCA'G
Cuts at: 830 2031 2109 2277 2639 2739 2934 3279 3575
Size: 1201 78 168 362 100 195 345 296
Cuts at: 3575 830
Size: 3478
Fragments arranged by size:
3478 1201 362 345 296 195 168 100
78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
PvuII CAG'CTG
Cuts at: 2113 2368 2376 2113
Size: 255 8 5960
Fragments arranged by size:
5960 255 8
SacI G_AGCT'C
Cuts at: 2811 2811
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SapI GCTCTTCn'nnn_
Cuts at: 2153 2189 2153
Size: 36 6187
SbfI CC_TGCA'GG
Cuts at: 2739 3575 2739
Size: 836 5387
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SexAI A'CCwGG_T
Cuts at: 3300 3300
Size: 6223
SfcI C'TryA_G
Cuts at: 826 1045 2027 2105 2273 2315 2635 2735 2930
Size: 219 982 78 168 42 320 100 195
Cuts at: 2930 3275 3571 4706 5387 5578 826
Size: 345 296 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 681 345 320 296 219
195 191 168 100 78 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 2066 3550 1872
Size: 194 1484 4545
Fragments arranged by size:
4545 1484 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4327 5228 5490 820
Size: 197 926 2384 901 262 1553
Fragments arranged by size:
2384 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3520 4014 493
Size: 3027 494 2702
Fragments arranged by size:
3027 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 2387 2585 1517
Size: 645 225 198 5155
Fragments arranged by size:
5155 645 225 198
StyI C'CwwG_G
Cuts at: 513 1065 3662 513
Size: 552 2597 3074
Fragments arranged by size:
3074 2597 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3400 4250 4267 4420 4605 5050 325
Size: 1255 1820 850 17 153 185 445 1498
Fragments arranged by size:
1820 1498 1255 850 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4469 6181 96
Size: 274 80 33 51 1443 2492 1712 138
Fragments arranged by size:
2492 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2525 2557 3442 3474 6183 6215 2525
Size: 32 885 32 2709 32 2533
Fragments arranged by size:
2709 2533 885 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6223
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6223
XmaI C'CCGG_G
Cuts at: 1870 2064 3548 1870
Size: 194 1484 4545
Fragments arranged by size:
4545 1484 194
XmnI GAAnn'nnTTC
Cuts at: 4352 4352
Size: 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI