Restriction map for
pFN21ASDA1949
(Circular) (Six-base) MAPSORT of: pFN21ASDA1949.seq Check: 6537 from: 1 to: 6568
pFN21ASDA1949 6568 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2686 3911 1937
Size: 749 1225 4594
Fragments arranged by size:
4594 1225 749
Acc65I G'GTAC_C
Cuts at: 3537 3889 3537
Size: 352 6216
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3631 4631 6015 6283 1632
Size: 1999 1000 1384 268 1917
Fragments arranged by size:
1999 1917 1384 1000 268
AflII C'TTAA_G
Cuts at: 820 1017 3635 820
Size: 197 2618 3753
Fragments arranged by size:
3753 2618 197
AflIII A'CryG_T
Cuts at: 1203 5467 6444 1203
Size: 4264 977 1327
Fragments arranged by size:
4264 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6568
AlwNI CAG_nnn'CTG
Cuts at: 4311 5883 4311
Size: 1572 4996
ApaI G_GGCC'C
Cuts at: 3415 3716 3415
Size: 301 6267
ApaLI G'TGCA_C
Cuts at: 2276 4625 5781 2276
Size: 2349 1156 3063
Fragments arranged by size:
3063 2349 1156
ApoI r'AATT_y
Cuts at: 1493 3877 4103 4255 6364 1493
Size: 2384 226 152 2109 1697
Fragments arranged by size:
2384 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 2529 2649 3045 3517 3893 1870
Size: 73 436 150 120 396 472 376 4545
Fragments arranged by size:
4545 472 436 396 376 150 120 73
BamHI G'GATC_C
Cuts at: 3358 3898 4265 3358
Size: 540 367 5661
Fragments arranged by size:
5661 540 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 3537 3889 5344 618
Size: 325 217 627 277 1473 352 1455 1842
Fragments arranged by size:
1842 1473 1455 627 352 325 277 217
BanII G_rGCy'C
Cuts at: 1391 2151 2423 2500 2573 2696 3178 3199 3291
Size: 760 272 77 73 123 482 21 92
Cuts at: 3291 3415 3716 3810 3887 1391
Size: 124 301 94 77 4072
Fragments arranged by size:
4072 760 482 301 272 124 123 94
92 77 77 73 21
BbeI G_GCGC'C
Cuts at: 2068 2068
Size: 6568
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5597
BbvCI CC'TCA_GC
Cuts at: 2810 2810
Size: 6568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4460 5670 4460
Size: 1210 5358
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5179 136
Size: 107 122 71 631 605 3507 1525
Fragments arranged by size:
3507 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3555 3639 6563 3555
Size: 84 2924 3560
Fragments arranged by size:
3560 2924 84
BlpI GC'TnA_GC
Cuts at: 3985 3985
Size: 6568
Bme1580I G_kGCm'C
Cuts at: 1790 2280 3222 3415 3465 3716 3739 4629 5785
Size: 490 942 193 50 251 23 890 1156
Cuts at: 5785 1790
Size: 2573
Fragments arranged by size:
2573 1156 942 890 490 251 193 50
23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4299 5257
Size: 416 53 83 399 24 2309 573 958
Cuts at: 5257 442
Size: 1753
Fragments arranged by size:
2309 1753 958 573 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2060 2351 1056
Size: 1004 291 5273
Fragments arranged by size:
5273 1004 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2214 2279 2393 2543 2557 2604 2631
Size: 935 40 65 114 150 14 47 27
Cuts at: 2631 2740 2810 3081 3162 3273 3275 3477 3683
Size: 109 70 271 81 111 2 202 206
Cuts at: 3683 3722 3732 3794 5228 1239
Size: 39 10 62 1434 2579
Fragments arranged by size:
2579 1434 935 271 206 202 150 114
111 109 81 70 65 62 47 40
39 27 14 10 2
Bpu10I CC'TnA_GC
Cuts at: 2810 3177 2810
Size: 367 6201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4693 5558 5856 4693
Size: 865 298 5405
Fragments arranged by size:
5405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2383 2459 2740 2906 3659 5231 882
Size: 617 884 76 281 166 753 1572 2219
Fragments arranged by size:
2219 1572 884 753 617 281 166 76
BsaAI yAC'GTr
Cuts at: 493 1204 3672 3865 4359 493
Size: 711 2468 193 494 2702
Fragments arranged by size:
2702 2468 711 494 193
BsaBI GATnn'nnATC
Cuts at: 6562 6562
Size: 6568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 2081 4375 4757
Size: 53 83 186 936 532 16 2294 382
Cuts at: 4757 5392 275
Size: 635 1451
Fragments arranged by size:
2294 1451 936 635 532 382 186 83
53 16
BsaWI w'CCGG_w
Cuts at: 5001 5399 5673 5820 6231 5001
Size: 398 274 147 411 5338
Fragments arranged by size:
5338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 2105 2133 2135
Size: 30 165 30 663 30 9 28 2
Cuts at: 2135 2163 2555 2585 2590 2620 3656 3686 1178
Size: 28 392 30 5 30 1036 30 4060
Fragments arranged by size:
4060 1036 663 392 165 30 30 30
30 30 30 28 28 9 5 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2491 3179 3566 3719 1186
Size: 904 401 688 387 153 4035
Fragments arranged by size:
4035 904 688 401 387 153
BseYI C'CCAG_C
Cuts at: 2393 2482 3245 3328 3475 3524 3709 5771 6267
Size: 89 763 83 147 49 185 2062 496
Cuts at: 6267 2393
Size: 2694
Fragments arranged by size:
2694 2062 763 496 185 147 89 83
49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5995
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4018 4779 5807 1540
Size: 275 186 2017 761 1028 2301
Fragments arranged by size:
2301 2017 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2280 2573 3291 3810 4629 4714 5785 2280
Size: 293 718 519 819 85 1071 3063
Fragments arranged by size:
3063 1071 819 718 519 293 85
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
BsmBI CGTCTCn'nnnn_
Cuts at: 3587 3587
Size: 6568
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2280 2423 2500 2573 2696 3178
Size: 399 361 129 143 77 73 123 482
Cuts at: 3178 3199 3222 3291 3415 3465 3716 3739 3810
Size: 21 23 69 124 50 251 23 71
Cuts at: 3810 3887 4629 4714 5785 1391
Size: 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 361 251 143
129 124 123 85 77 77 73 71
69 50 23 23 21
BspEI T'CCGG_A
Cuts at: 6231 6231
Size: 6568
BspHI T'CATG_A
Cuts at: 3366 4457 6187 3366
Size: 1091 1730 3747
Fragments arranged by size:
3747 1730 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3923 844
Size: 3079 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 2386 2626 4455 1394
Size: 429 482 81 240 1829 3507
Fragments arranged by size:
3507 1829 482 429 240 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 5063 5237 65
Size: 1183 3815 174 1396
Fragments arranged by size:
3815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 3855 5212 5399 1700
Size: 110 257 1788 1357 187 2869
Fragments arranged by size:
2869 1788 1357 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 4628 5640 4628
Size: 1012 5556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6568
BstBI TT'CG_AA
Cuts at: 2007 5372 2007
Size: 3365 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6568
BstXI CCAn_nnnn'nTGG
Cuts at: 3353 3353
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 3358 3555 3639 3898 4265 4650 4667 6119
Size: 1386 197 84 259 367 385 17 1452
Cuts at: 6119 6205 6217 6563 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1386 385 367 346 259 197
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2513 3723 2513
Size: 1210 5358
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5059
Fragments arranged by size:
5059 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5829
Fragments arranged by size:
5829 683 56
BtsI GCAGTG_nn'
Cuts at: 3288 3430 4084 4878 4898 3288
Size: 142 654 794 20 4958
Fragments arranged by size:
4958 794 654 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2867 4015
Size: 54 1425 50 275 18 195 842 1148
Cuts at: 4015 4904 6533 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1148 889 842 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4015 1537
Size: 275 2203 4090
Fragments arranged by size:
4090 2203 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 2425 2439 2856 3267 3765 3777 4498
Size: 981 91 14 417 411 498 12 721
Cuts at: 4498 1353
Size: 3423
Fragments arranged by size:
3423 981 721 498 417 411 91 14
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5155 5529 5675 387
Size: 1064 138 3566 374 146 1280
Fragments arranged by size:
3566 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2571 3289 3808 2571
Size: 718 519 5331
Fragments arranged by size:
5331 718 519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2214 2279 2393 2543 2557 2604
Size: 393 542 40 65 114 150 14 47
Cuts at: 2604 2631 2740 2810 3081 3162 3273 3275 3477
Size: 27 109 70 271 81 111 2 202
Cuts at: 3477 3631 3683 3722 3732 3794 4631 5228 6015
Size: 154 52 39 10 62 837 597 787
Cuts at: 6015 6283 1239
Size: 268 1524
Fragments arranged by size:
1524 837 787 597 542 393 271 268
202 154 150 114 111 109 81 70
65 62 52 47 40 39 27 14
10 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2205 2258 2729 3602 2205
Size: 53 471 873 5171
Fragments arranged by size:
5171 873 471 53
EcoO109I rG'GnC_Cy
Cuts at: 3411 3412 3609 3727 3832 3411
Size: 1 197 118 105 6147
Fragments arranged by size:
6147 197 118 105 1
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 6568
FspI TGC'GCA
Cuts at: 3824 5074 3824
Size: 1250 5318
HaeII r_GCGC'y
Cuts at: 1678 2068 2119 2161 5715 1678
Size: 390 51 42 3554 2531
Fragments arranged by size:
3554 2531 390 51 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2105 2137 2398
Size: 32 135 32 494 32 7 32 261
Cuts at: 2398 2430 2440 2472 2555 2587 2590 2622 5209
Size: 32 10 32 83 32 3 32 2587
Cuts at: 5209 5241 5283 5315 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2587 494 261 135 83 42 32
32 32 32 32 32 32 32 32
32 10 7 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3445 3912 4158 669
Size: 988 281 1507 467 246 3079
Fragments arranged by size:
3079 1507 988 467 281 246
HindIII A'AGCT_T
Cuts at: 748 2171 3113 748
Size: 1423 942 4203
Fragments arranged by size:
4203 1423 942
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KasI G'GCGC_C
Cuts at: 2064 2064
Size: 6568
KpnI G_GTAC'C
Cuts at: 3541 3893 3541
Size: 352 6216
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5682 5866 1305
Size: 4377 184 2007
Fragments arranged by size:
4377 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2869 10
Size: 54 1425 343 195 842 3709
Fragments arranged by size:
3709 1425 842 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2593 4526 4885 5044 518
Size: 648 1427 1933 359 159 2042
Fragments arranged by size:
2042 1933 1427 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 3594 3984 4661 5809 6054 1606
Size: 447 1541 390 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 447 390 245
NaeI GCC'GGC
Cuts at: 2069 3857 2069
Size: 1788 4780
NarI GG'CG_CC
Cuts at: 2065 2065
Size: 6568
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5059
Fragments arranged by size:
5059 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NgoMIV G'CCGG_C
Cuts at: 2067 3855 2067
Size: 1788 4780
NheI G'CTAG_C
Cuts at: 1052 2056 2347 1052
Size: 1004 291 5273
Fragments arranged by size:
5273 1004 291
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 3926 5471 1132
Size: 97 2697 1545 2229
Fragments arranged by size:
2697 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2186 2399 2696 3060 1348
Size: 838 213 297 364 4856
Fragments arranged by size:
4856 838 364 297 213
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2719 1108
Size: 1611 4957
PfoI T'CCnGG_A
Cuts at: 2151 2608 3035 2151
Size: 457 427 5684
Fragments arranged by size:
5684 457 427
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 4637 4669 6150 6182 3197
Size: 32 1408 32 1481 32 3583
Fragments arranged by size:
3583 1481 1408 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3609 3727 3609
Size: 118 6450
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PspOMI G'GGCC_C
Cuts at: 3411 3712 3411
Size: 301 6267
PstI C_TGCA'G
Cuts at: 830 2902 3204 3514 3801 3920 830
Size: 2072 302 310 287 119 3478
Fragments arranged by size:
3478 2072 310 302 287 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2053 3594 2053
Size: 1541 5027
SacI G_AGCT'C
Cuts at: 2573 3291 3810 2573
Size: 718 519 5331
Fragments arranged by size:
5331 718 519
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SanDI GG'GwC_CC
Cuts at: 3609 3609
Size: 6568
SapI GCTCTTCn'nnn_
Cuts at: 2334 3777 2334
Size: 1443 5125
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SfcI C'TryA_G
Cuts at: 826 1045 2048 2214 2898 3200 3510 3797 3916
Size: 219 1003 166 684 302 310 287 119
Cuts at: 3916 5051 5732 5923 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 684 681 310 302 287
219 191 166 119
SfoI GGC'GCC
Cuts at: 2066 2066
Size: 6568
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 2381 3519 3895 1872
Size: 509 1138 376 4545
Fragments arranged by size:
4545 1138 509 376
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2649 3045 3635 4672 5573 5835
Size: 197 926 706 396 590 1037 901 262
Cuts at: 5835 820
Size: 1553
Fragments arranged by size:
1553 1037 926 901 706 590 396 262
197
SnaBI TAC'GTA
Cuts at: 493 3865 4359 493
Size: 3372 494 2702
Fragments arranged by size:
3372 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 4492 5
Size: 47 4440 2081
Fragments arranged by size:
4440 2081 47
StuI AGG'CCT
Cuts at: 1517 2518 2974 3187 3258 1517
Size: 1001 456 213 71 4827
Fragments arranged by size:
4827 1001 456 213 71
StyI C'CwwG_G
Cuts at: 513 1065 2022 2424 2932 3603 3828 4007 513
Size: 552 957 402 508 671 225 179 3074
Fragments arranged by size:
3074 957 671 552 508 402 225 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3073 4595 4612 4765 4950 5395 325
Size: 1255 1493 1522 17 153 185 445 1498
Fragments arranged by size:
1522 1498 1493 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4814 6526 96
Size: 274 80 33 51 1443 2837 1712 138
Fragments arranged by size:
2837 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6528 6560 6528
Size: 32 6536
Tth111I GACn'n_nGTC
Cuts at: 1316 2454 1316
Size: 1138 5430
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3012 3012
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 2649 3045 1943
Size: 706 396 5466
Fragments arranged by size:
5466 706 396
XmaI C'CCGG_G
Cuts at: 1870 2379 3517 3893 1870
Size: 509 1138 376 4545
Fragments arranged by size:
4545 1138 509 376
XmnI GAAnn'nnTTC
Cuts at: 2288 4697 2288
Size: 2409 4159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI
SrfI SwaI