Restriction map for
pFN21ASDA1934
(Circular) (Six-base) MAPSORT of: pFN21ASDA1934.seq Check: 6767 from: 1 to: 6073
pFN21ASDA1934 6073 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2335 3082 3416 1937
Size: 398 747 334 4594
Fragments arranged by size:
4594 747 398 334
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4136 5520 5788 1632
Size: 2504 1384 268 1917
Fragments arranged by size:
2504 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 2158 3332 3816 5388 2158
Size: 1174 484 1572 2843
Fragments arranged by size:
2843 1572 1174 484
ApaLI G'TGCA_C
Cuts at: 2843 4130 5286 2843
Size: 1287 1156 3630
Fragments arranged by size:
3630 1287 1156
ApoI r'AATT_y
Cuts at: 1493 2124 3382 3608 3760 5869 1493
Size: 631 1258 226 152 2109 1697
Fragments arranged by size:
2109 1697 1258 631 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2904 3398 1870
Size: 73 961 494 4545
Fragments arranged by size:
4545 961 494 73
AvrII C'CTAG_G
Cuts at: 2176 2176
Size: 6073
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3394 4849 618
Size: 325 217 627 1607 1455 1842
Fragments arranged by size:
1842 1607 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2392 2826 2855 2946 3392 1391
Size: 1001 434 29 91 446 4072
Fragments arranged by size:
4072 1001 446 434 91 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 2209 2556 3240 928
Size: 971 306 4 347 684 3761
Fragments arranged by size:
3761 971 684 347 306 4
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3264 3965 5175 3264
Size: 701 1210 4162
Fragments arranged by size:
4162 1210 701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2587 4684 136
Size: 107 122 71 631 605 915 2097 1525
Fragments arranged by size:
2097 1525 915 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2721 6068 2721
Size: 3347 2726
BlpI GC'TnA_GC
Cuts at: 3490 3490
Size: 6073
Bme1580I G_kGCm'C
Cuts at: 1790 2847 4134 5290 1790
Size: 1057 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 1057
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 3804 4762
Size: 416 53 83 399 24 836 1551 958
Cuts at: 4762 442
Size: 1753
Fragments arranged by size:
1753 1551 958 836 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2645 2651 2960 2999 3261 4733 1239
Size: 1406 6 309 39 262 1472 2579
Fragments arranged by size:
2579 1472 1406 309 262 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2534 4736 882
Size: 617 1035 2202 2219
Fragments arranged by size:
2219 2202 1035 617
BsaAI yAC'GTr
Cuts at: 493 1204 2636 3252 3370 3864 493
Size: 711 1432 616 118 494 2702
Fragments arranged by size:
2702 1432 711 616 494 118
BsaBI GATnn'nnATC
Cuts at: 6067 6067
Size: 6073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2709 3880 4262 4897
Size: 53 83 186 936 1176 1171 382 635
Cuts at: 4897 275
Size: 1451
Fragments arranged by size:
1451 1176 1171 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3077 4506 4904 5178 5325 5736 3077
Size: 1429 398 274 147 411 3414
Fragments arranged by size:
3414 1429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2666 2696 2781 2811 1178
Size: 30 165 30 1263 30 85 30 4440
Fragments arranged by size:
4440 1263 165 85 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3323 1186
Size: 2137 3936
BseYI C'CCAG_C
Cuts at: 3332 5276 5772 3332
Size: 1944 496 3633
Fragments arranged by size:
3633 1944 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3096 3149 1163
Size: 573 1360 53 4087
Fragments arranged by size:
4087 1360 573 53
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2667 2847 2946 4134 4219 5290 2667
Size: 180 99 1188 85 1071 3450
Fragments arranged by size:
3450 1188 1071 180 99 85
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2667 2826 2847 2855 2946 3392
Size: 399 602 275 159 21 8 91 446
Cuts at: 3392 4134 4219 5290 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 446 399 275 159
91 85 21 8
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2554 3428 844
Size: 1710 874 3489
Fragments arranged by size:
3489 1710 874
BsrBI CCG'CTC
Cuts at: 1394 1823 3960 1394
Size: 429 2137 3507
Fragments arranged by size:
3507 2137 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4568 4742 65
Size: 1183 3320 174 1396
Fragments arranged by size:
3320 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 2007 4877 2007
Size: 2870 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2692 2977 2392
Size: 300 285 5488
Fragments arranged by size:
5488 300 285
BstYI r'GATC_y
Cuts at: 1972 2450 2721 3403 3770 4155 4172 5624 5710
Size: 478 271 682 367 385 17 1452 86
Cuts at: 5710 5722 6068 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 682 478 385 367 346 271
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2927 3117 2927
Size: 190 5883
BtgI C'CryG_G
Cuts at: 513 1065 2022 2103 3238 513
Size: 552 957 81 1135 3348
Fragments arranged by size:
3348 1135 957 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5334
Fragments arranged by size:
5334 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2705 3036 3220 3589 4383 4403 2033
Size: 672 331 184 369 794 20 3703
Fragments arranged by size:
3703 794 672 369 331 184 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 3241 3520
Size: 54 1425 50 275 18 552 859 279
Cuts at: 3520 4409 6038 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 859 552 279 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2532 2603 3003 4003 1353
Size: 1179 71 400 1000 3423
Fragments arranged by size:
3423 1179 1000 400 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2944 2944
Size: 6073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2645 2651 2960 2999 3261 4136 4733
Size: 393 1013 6 309 39 262 875 597
Cuts at: 4733 5520 5788 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1013 875 787 597 393 309 268
262 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2529 2529
Size: 6073
EcoO109I rG'GnC_Cy
Cuts at: 2080 2173 3072 2080
Size: 93 899 5081
Fragments arranged by size:
5081 899 93
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2233 2740 2872 2164
Size: 69 507 132 5365
Fragments arranged by size:
5365 507 132 69
FspI TGC'GCA
Cuts at: 2164 2867 4579 2164
Size: 703 1712 3658
Fragments arranged by size:
3658 1712 703
FspAI rTGC'GCAy
Cuts at: 2164 2164
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 5220 1678
Size: 3542 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2781 2813 3038 3070 4714
Size: 32 135 32 1209 32 225 32 1644
Cuts at: 4714 4746 4788 4820 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1644 1209 225 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3663 669
Size: 988 281 1479 246 3079
Fragments arranged by size:
3079 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5187 5371 1305
Size: 3882 184 2007
Fragments arranged by size:
3882 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 3243 10
Size: 54 1425 343 552 859 2840
Fragments arranged by size:
2840 1425 859 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2660 2690 2876 4031 4390 4549
Size: 648 936 558 30 186 1155 359 159
Cuts at: 4549 518
Size: 2042
Fragments arranged by size:
2042 1155 936 648 558 359 186 159
30
MspA1I CmG'CkG
Cuts at: 1606 3489 4166 5314 5559 1606
Size: 1883 677 1148 245 2120
Fragments arranged by size:
2120 1883 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2103 3238 513
Size: 552 957 81 1135 3348
Fragments arranged by size:
3348 1135 957 552 81
NdeI CA'TA_TG
Cuts at: 387 2585 387
Size: 2198 3875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 3431 4976 1132
Size: 97 2202 1545 2229
Fragments arranged by size:
2229 2202 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2392 3357 1348
Size: 1044 965 4064
Fragments arranged by size:
4064 1044 965
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3357 1108
Size: 2249 3824
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2173
Size: 6073
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PstI C_TGCA'G
Cuts at: 830 2772 2835 3304 3425 830
Size: 1942 63 469 121 3478
Fragments arranged by size:
3478 1942 469 121 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
SacI G_AGCT'C
Cuts at: 2946 2946
Size: 6073
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SfcI C'TryA_G
Cuts at: 826 1045 2768 2831 3300 3421 4556 5237 5428
Size: 219 1723 63 469 121 1135 681 191
Cuts at: 5428 826
Size: 1471
Fragments arranged by size:
1723 1471 1135 681 469 219 191 121
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3400 1872
Size: 1528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4177 5078 5340 820
Size: 197 926 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3370 3864 493
Size: 2877 494 2702
Fragments arranged by size:
2877 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 2022 2103 2176 2208 3238 3258 3512
Size: 552 957 81 73 32 1030 20 254
Cuts at: 3512 513
Size: 3074
Fragments arranged by size:
3074 1030 957 552 254 81 73 32
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4100 4117 4270 4455 4900 325
Size: 1255 2520 17 153 185 445 1498
Fragments arranged by size:
2520 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4319 6031 96
Size: 274 80 33 51 1443 2342 1712 138
Fragments arranged by size:
2342 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 2237 1316
Size: 921 5152
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 3398 1870
Size: 1528 4545
XmnI GAAnn'nnTTC
Cuts at: 2345 2518 4202 2345
Size: 173 1684 4216
Fragments arranged by size:
4216 1684 173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI