Restriction map for
pFN21ASDA1916
(Circular) (Six-base) MAPSORT of: pFN21ASDA1916.seq Check: 858 from: 1 to: 6364
pFN21ASDA1916 6364 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2098 2098
Size: 6364
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2464 4427 5811 6079 1632
Size: 832 1963 1384 268 1917
Fragments arranged by size:
1963 1917 1384 832 268
AfeI AGC'GCT
Cuts at: 2052 2052
Size: 6364
AflII C'TTAA_G
Cuts at: 820 1017 3649 820
Size: 197 2632 3535
Fragments arranged by size:
3535 2632 197
AflIII A'CryG_T
Cuts at: 1203 5263 6240 1203
Size: 4060 977 1327
Fragments arranged by size:
4060 1327 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6364
AlwNI CAG_nnn'CTG
Cuts at: 3121 4107 5679 3121
Size: 986 1572 3806
Fragments arranged by size:
3806 1572 986
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 2299 2525 2578 2815 2954 2963 3673 3899
Size: 806 226 53 237 139 9 710 226
Cuts at: 3899 4051 6160 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 806 710 237 226 226 152
139 53 9
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 3351 3405 3689 1870
Size: 73 111 1297 54 284 4545
Fragments arranged by size:
4545 1297 284 111 73 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2603 2636 2603
Size: 33 6331
BamHI G'GATC_C
Cuts at: 3694 4061 3694
Size: 367 5997
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2185 3547 3685 5140
Size: 325 217 627 291 107 1362 138 1455
Cuts at: 5140 618
Size: 1842
Fragments arranged by size:
1842 1455 1362 627 325 291 217 138
107
BanII G_rGCy'C
Cuts at: 1391 2177 3683 1391
Size: 786 1506 4072
Fragments arranged by size:
4072 1506 786
BbeI G_GCGC'C
Cuts at: 2082 3551 2082
Size: 1469 4895
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2470 928
Size: 971 571 4822
Fragments arranged by size:
4822 971 571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3533 3567 4555 4589 1903
Size: 34 1596 34 988 34 3678
Fragments arranged by size:
3678 1596 988 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2716 4256 5466 2716
Size: 1540 1210 3614
Fragments arranged by size:
3614 1540 1210
BclI T'GATC_A
Cuts at: 1548 2881 1548
Size: 1333 5031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4975 136
Size: 107 122 71 631 605 3303 1525
Fragments arranged by size:
3303 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 3781 3781
Size: 6364
Bme1580I G_kGCm'C
Cuts at: 1790 2190 4425 5581 1790
Size: 400 2235 1156 2573
Fragments arranged by size:
2573 2235 1156 400
BmgBI CAC'GTC
Cuts at: 1318 2644 1318
Size: 1326 5038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4095 5053 442
Size: 416 53 83 399 24 2678 958 1753
Fragments arranged by size:
2678 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 3203 5024 1239
Size: 1574 390 1821 2579
Fragments arranged by size:
2579 1821 1574 390
Bpu10I CC'TnA_GC
Cuts at: 2219 2219
Size: 6364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4489 5354 5652 4489
Size: 865 298 5201
Fragments arranged by size:
5201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2986 5027 882
Size: 617 1487 2041 2219
Fragments arranged by size:
2219 2041 1487 617
BsaAI yAC'GTr
Cuts at: 493 1204 2752 3661 4155 493
Size: 711 1548 909 494 2702
Fragments arranged by size:
2702 1548 909 711 494
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3548 4171 4553
Size: 53 83 186 936 546 1469 623 382
Cuts at: 4553 5188 275
Size: 635 1451
Fragments arranged by size:
1469 1451 936 635 623 546 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2820 4797 5195 5469 5616 6027 2820
Size: 1977 398 274 147 411 3157
Fragments arranged by size:
3157 1977 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 5314
Fragments arranged by size:
5314 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6364
BseYI C'CCAG_C
Cuts at: 2074 2768 2904 5567 6063 2074
Size: 694 136 2663 496 2375
Fragments arranged by size:
2663 2375 694 496 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3107 3563 1163
Size: 573 1371 456 3964
Fragments arranged by size:
3964 1371 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3814 4575 5603 1540
Size: 275 186 1813 761 1028 2301
Fragments arranged by size:
2301 1813 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4425 4510 5581 4425
Size: 85 1071 5208
Fragments arranged by size:
5208 1071 85
BsmI GAATG_Cn'
Cuts at: 2669 3309 3875 3968 2669
Size: 640 566 93 5065
Fragments arranged by size:
5065 640 566 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3026 3026
Size: 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2190 3683 4425 4510 5581 1391
Size: 399 387 13 1493 742 85 1071 2174
Fragments arranged by size:
2174 1493 1071 742 399 387 85 13
BspEI T'CCGG_A
Cuts at: 2820 6027 2820
Size: 3207 3157
BspHI T'CATG_A
Cuts at: 3357 4253 5983 3357
Size: 896 1730 3738
Fragments arranged by size:
3738 1730 896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2098 3719 844
Size: 1254 1621 3489
Fragments arranged by size:
3489 1621 1254
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 4251 1394
Size: 429 1375 1053 3507
Fragments arranged by size:
3507 1375 1053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5008 5195 1700
Size: 110 3198 187 2869
Fragments arranged by size:
3198 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 2103 2123 1925
Size: 178 20 6166
Fragments arranged by size:
6166 178 20
BssSI C'ACGA_G
Cuts at: 3125 4424 5436 3125
Size: 1299 1012 4053
Fragments arranged by size:
4053 1299 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 2007 5168 2007
Size: 3161 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstYI r'GATC_y
Cuts at: 1972 3387 3694 4061 4446 4463 5915 6001 6013
Size: 1415 307 367 385 17 1452 86 12
Cuts at: 6013 6359 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1415 385 367 346 307 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2096 3253 513
Size: 552 957 74 1157 3624
Fragments arranged by size:
3624 1157 957 552 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2743 3031 487
Size: 683 56 1517 288 3820
Fragments arranged by size:
3820 1517 683 288 56
BtsI GCAGTG_nn'
Cuts at: 3119 3880 4674 4694 3119
Size: 761 794 20 4789
Fragments arranged by size:
4789 794 761 20
ClaI AT'CG_AT
Cuts at: 1554 2877 3165 1554
Size: 1323 288 4753
Fragments arranged by size:
4753 1323 288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3439 3668 3439
Size: 229 6135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2978 3353 5371 809
Size: 507 1662 375 2018 1802
Fragments arranged by size:
2018 1802 1662 507 375
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2754 3811
Size: 54 1425 50 275 18 612 312 1057
Cuts at: 3811 4700 6329 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1057 889 612 312 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3811 1537
Size: 275 1999 4090
Fragments arranged by size:
4090 1999 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 2915 3287 3402 4294 1353
Size: 829 733 372 115 892 3423
Fragments arranged by size:
3423 892 829 733 372 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4951 5325 5471 387
Size: 1064 138 3362 374 146 1280
Fragments arranged by size:
3362 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2464 2813 3203 4427 5024 5811 6079
Size: 393 832 349 390 1224 597 787 268
Cuts at: 6079 1239
Size: 1524
Fragments arranged by size:
1524 1224 832 787 597 393 390 349
268
EcoO109I rG'GnC_Cy
Cuts at: 3205 3205
Size: 6364
EcoRI G'AATT_C
Cuts at: 2299 3673 4051 2299
Size: 1374 378 4612
Fragments arranged by size:
4612 1374 378
EcoRV GAT'ATC
Cuts at: 3163 3163
Size: 6364
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 2030 2054 2082 3551 5511 1678
Size: 352 24 28 1469 1960 2531
Fragments arranged by size:
2531 1960 1469 352 28 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2477 2509 3158
Size: 32 135 32 626 32 247 32 649
Cuts at: 3158 3190 3379 3411 5005 5037 5079 5111 1373
Size: 32 189 32 1594 32 42 32 2626
Fragments arranged by size:
2626 1594 649 626 247 189 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3646 3708 3954 669
Size: 988 281 1708 62 246 3079
Fragments arranged by size:
3079 1708 988 281 246 62
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 2078 3547 2078
Size: 1469 4895
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2927 5478 5662 1305
Size: 1622 2551 184 2007
Fragments arranged by size:
2551 2007 1622 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 2756 10
Size: 54 1425 343 612 312 3618
Fragments arranged by size:
3618 1425 612 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2810 4322 4681 4840 518
Size: 648 1002 642 1512 359 159 2042
Fragments arranged by size:
2042 1512 1002 648 642 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2145 2287 3566 3780 4457 5605 5850
Size: 492 47 142 1279 214 677 1148 245
Cuts at: 5850 1606
Size: 2120
Fragments arranged by size:
2120 1279 1148 677 492 245 214 142
47
NarI GG'CG_CC
Cuts at: 2079 3548 2079
Size: 1469 4895
NcoI C'CATG_G
Cuts at: 513 1065 2022 3253 513
Size: 552 957 1231 3624
Fragments arranged by size:
3624 1231 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 3722 5267 1132
Size: 97 2493 1545 2229
Fragments arranged by size:
2493 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3460 1348
Size: 2112 4252
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6364
PfoI T'CCnGG_A
Cuts at: 2322 2322
Size: 6364
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3205 3205
Size: 6364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3084 3934 3084
Size: 850 5514
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 6332
PstI C_TGCA'G
Cuts at: 830 2286 3426 3716 830
Size: 1456 1140 290 3478
Fragments arranged by size:
3478 1456 1140 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
PvuII CAG'CTG
Cuts at: 2145 2287 3566 2145
Size: 142 1279 4943
Fragments arranged by size:
4943 1279 142
SacII CC_GC'GG
Cuts at: 2099 2099
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SapI GCTCTTCn'nnn_
Cuts at: 2182 2915 3287 3402 2182
Size: 733 372 115 5144
Fragments arranged by size:
5144 733 372 115
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2282 2673 3422 3712 4847 5528 5719
Size: 219 1237 391 749 290 1135 681 191
Cuts at: 5719 826
Size: 1471
Fragments arranged by size:
1471 1237 1135 749 681 391 290 219
191
SfoI GGC'GCC
Cuts at: 2080 3549 2080
Size: 1469 4895
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3353 3407 3691 1872
Size: 1481 54 284 4545
Fragments arranged by size:
4545 1481 284 54
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3649 4468 5369 5631 820
Size: 197 926 1706 819 901 262 1553
Fragments arranged by size:
1706 1553 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 3661 4155 493
Size: 3168 494 2702
Fragments arranged by size:
3168 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2022 2151 2468 3253 3803 513
Size: 552 957 129 317 785 550 3074
Fragments arranged by size:
3074 957 785 552 550 317 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 2271 4391 4408 4561 4746 5191
Size: 1255 593 98 2120 17 153 185 445
Cuts at: 5191 325
Size: 1498
Fragments arranged by size:
2120 1498 1255 593 445 185 153 98
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4610 6322 96
Size: 274 80 33 51 1443 2633 1712 138
Fragments arranged by size:
2633 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6324 6356 6324
Size: 32 6332
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2912 2912
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3351 3405 3689 1870
Size: 1481 54 284 4545
Fragments arranged by size:
4545 1481 284 54
XmnI GAAnn'nnTTC
Cuts at: 2263 3296 4493 2263
Size: 1033 1197 4134
Fragments arranged by size:
4134 1197 1033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI RsrII SacI SanDI SexAI SfiI SrfI SwaI