Restriction map for
pFN21ASDA1912
(Circular) (Six-base) MAPSORT of: pFN21ASDA1912.seq Check: 8954 from: 1 to: 6388
pFN21ASDA1912 6388 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2739 2739
Size: 6388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3246 3731 1937
Size: 1309 485 4594
Fragments arranged by size:
4594 1309 485
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3023 3523 4451 5835 6103 1632
Size: 1391 500 928 1384 268 1917
Fragments arranged by size:
1917 1391 1384 928 500 268
AflII C'TTAA_G
Cuts at: 820 1017 3590 820
Size: 197 2573 3618
Fragments arranged by size:
3618 2573 197
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 2982 5219 2982
Size: 2237 4151
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3499 3531 3499
Size: 32 6356
AlwNI CAG_nnn'CTG
Cuts at: 2335 2407 2464 2574 2814 3500 4131 5703 2335
Size: 72 57 110 240 686 631 1572 3020
Fragments arranged by size:
3020 1572 686 631 240 110 72 57
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 3697 3923 4075 6184 1493
Size: 2204 226 152 2109 1697
Fragments arranged by size:
2204 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2194 2194
Size: 6388
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 2789 3472 3713 1870
Size: 73 448 398 683 241 4545
Fragments arranged by size:
4545 683 448 398 241 73
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2904 3089 3194 3438 3709
Size: 325 217 627 1117 185 105 244 271
Cuts at: 3709 5164 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1117 627 325 271 244 217
185 105
BanII G_rGCy'C
Cuts at: 1391 2517 2526 2963 3029 3131 3412 3707 1391
Size: 1126 9 437 66 102 281 295 4072
Fragments arranged by size:
4072 1126 437 295 281 102 66 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 928
Size: 971 429 4988
Fragments arranged by size:
4988 971 429
BbvCI CC'TCA_GC
Cuts at: 2080 2080
Size: 6388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4280 5490 4280
Size: 1210 5178
BclI T'GATC_A
Cuts at: 1548 3133 1548
Size: 1585 4803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3805 3805
Size: 6388
Bme1580I G_kGCm'C
Cuts at: 1790 3092 3197 4449 5605 1790
Size: 1302 105 1252 1156 2573
Fragments arranged by size:
2573 1302 1252 1156 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2417 4119 5077
Size: 416 53 83 399 24 1000 1702 958
Cuts at: 5077 442
Size: 1753
Fragments arranged by size:
1753 1702 1000 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3537 1056
Size: 2481 3907
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2406 2406
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2495 2647 3311 3332 5048 1239
Size: 1061 195 152 664 21 1716 2579
Fragments arranged by size:
2579 1716 1061 664 195 152 21
Bpu10I CC'TnA_GC
Cuts at: 2080 2080
Size: 6388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2501 4513 5378 5676 2279
Size: 222 2012 865 298 2991
Fragments arranged by size:
2991 2012 865 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5051 882
Size: 617 3552 2219
Fragments arranged by size:
3552 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2632 2947 3685 4179 493
Size: 711 1428 315 738 494 2702
Fragments arranged by size:
2702 1428 738 711 494 315
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2982 3237 4821 5219 5493 5640 6051 2982
Size: 255 1584 398 274 147 411 3319
Fragments arranged by size:
3319 1584 411 398 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 3209 3239 3499
Size: 30 165 30 711 30 1065 30 260
Cuts at: 3499 3529 1178
Size: 30 4037
Fragments arranged by size:
4037 1065 711 260 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2186 2558 3162 1186
Size: 976 24 372 604 4412
Fragments arranged by size:
4412 976 604 372 24
BseYI C'CCAG_C
Cuts at: 3396 3561 5591 6087 3396
Size: 165 2030 496 3697
Fragments arranged by size:
3697 2030 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2574 1163
Size: 573 519 319 4977
Fragments arranged by size:
4977 573 519 319
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2091 3838 4599 5627 1540
Size: 275 186 36 54 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186 54 36
BsiHKAI G_wGCw'C
Cuts at: 2517 2526 2730 2766 2865 3105 4449 4534 5605
Size: 9 204 36 99 240 1344 85 1071
Cuts at: 5605 2517
Size: 3300
Fragments arranged by size:
3300 1344 1071 240 204 99 85 36
9
BsmI GAATG_Cn'
Cuts at: 3899 3992 3899
Size: 93 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2517 2526 2730 2766 2865 2963 3029
Size: 399 727 9 204 36 99 98 66
Cuts at: 3029 3092 3105 3131 3197 3412 3707 4449 4534
Size: 63 13 26 66 215 295 742 85
Cuts at: 4534 5605 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 727 399 295 215 204
99 98 85 66 66 63 36 26
13 9
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2463 2739 3614 3743 844
Size: 1619 276 875 129 3489
Fragments arranged by size:
3489 1619 875 276 129
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 5057 65
Size: 1183 3635 174 1396
Fragments arranged by size:
3635 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 2116 2367 2982 5032 5219 1700
Size: 110 280 26 251 615 2050 187 2869
Fragments arranged by size:
2869 2050 615 280 251 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 2194 1925
Size: 269 6119
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2574 794
Size: 1780 4608
BstBI TT'CG_AA
Cuts at: 2007 5192 2007
Size: 3185 3203
BstEII G'GTnAC_C
Cuts at: 1184 3225 1184
Size: 2041 4347
BstYI r'GATC_y
Cuts at: 1972 2283 3718 4085 4470 4487 5939 6025 6037
Size: 311 1435 367 385 17 1452 86 12
Cuts at: 6037 6383 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1435 385 367 346 311 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2100 2287 513
Size: 552 1035 187 4614
Fragments arranged by size:
4614 1035 552 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2234 487
Size: 683 56 1008 4641
Fragments arranged by size:
4641 1008 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 2418 2719 3160 3431 3904 4698 4718 2165
Size: 253 301 441 271 473 794 20 3835
Fragments arranged by size:
3835 794 473 441 301 271 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2088 3835
Size: 54 1425 50 275 18 204 54 1747
Cuts at: 3835 4724 6353 8
Size: 889 1629 43
Fragments arranged by size:
1747 1629 1425 889 275 204 54 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 2088 3835 1537
Size: 275 222 54 1747 4090
Fragments arranged by size:
4090 1747 275 222 54
EarI CTCTTCn'nnn_
Cuts at: 1353 4318 1353
Size: 2965 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 2219 3192 4975 5349 5495
Size: 1064 138 492 138 973 1783 374 146
Cuts at: 5495 387
Size: 1280
Fragments arranged by size:
1783 1280 1064 973 492 374 146 138
138
EcoICRI GAG'CTC
Cuts at: 2515 2524 2515
Size: 9 6379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2495 2647 3023 3311 3332 3523
Size: 393 668 195 152 376 288 21 191
Cuts at: 3523 4451 5048 5835 6103 1239
Size: 928 597 787 268 1524
Fragments arranged by size:
1524 928 787 668 597 393 376 288
268 195 191 152 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3280 3280
Size: 6388
EcoO109I rG'GnC_Cy
Cuts at: 2125 2341 2849 2125
Size: 216 508 5664
Fragments arranged by size:
5664 508 216
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
EcoRV GAT'ATC
Cuts at: 2302 2302
Size: 6388
FspI TGC'GCA
Cuts at: 2563 4894 2563
Size: 2331 4057
HaeII r_GCGC'y
Cuts at: 1678 2784 5535 1678
Size: 1106 2751 2531
Fragments arranged by size:
2751 2531 1106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2395 2427 3118
Size: 32 135 32 542 32 249 32 691
Cuts at: 3118 3150 3209 3241 5029 5061 5103 5135 1373
Size: 32 59 32 1788 32 42 32 2626
Fragments arranged by size:
2626 1788 691 542 249 135 59 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3732 3978 669
Size: 988 281 1794 246 3079
Fragments arranged by size:
3079 1794 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 3389 5502 5686 1305
Size: 816 1268 2113 184 2007
Fragments arranged by size:
2113 2007 1268 816 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4566
Fragments arranged by size:
4566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3240 4346 4705 4864 518
Size: 648 2074 1106 359 159 2042
Fragments arranged by size:
2074 2042 1106 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2269 2289 2574 3174 3289 3310 3804
Size: 496 167 20 285 600 115 21 494
Cuts at: 3804 4481 5629 5874 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 496 494 285 245
167 115 21 20
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 6388
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5836
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 3533 1052
Size: 2481 3907
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3331 3551 3746 5291 1132
Size: 97 2102 220 195 1545 2229
Fragments arranged by size:
2229 2102 1545 220 195 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6388
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6388
PfoI T'CCnGG_A
Cuts at: 2316 2355 3634 2316
Size: 39 1279 5070
Fragments arranged by size:
5070 1279 39
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PmlI CAC'GTG
Cuts at: 2947 2947
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3531 4457 4489 5970 6002 3499
Size: 32 926 32 1481 32 3885
Fragments arranged by size:
3885 1481 926 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2341 2849 2125
Size: 216 508 5664
Fragments arranged by size:
5664 508 216
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3564 3596 3564
Size: 32 6356
PstI C_TGCA'G
Cuts at: 830 2160 2268 2460 2736 3532 3625 3740 830
Size: 1330 108 192 276 796 93 115 3478
Fragments arranged by size:
3478 1330 796 276 192 115 108 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2269 3289 3310 2269
Size: 1020 21 5347
Fragments arranged by size:
5347 1020 21
SacI G_AGCT'C
Cuts at: 2517 2526 2517
Size: 9 6379
SacII CC_GC'GG
Cuts at: 2103 2290 2103
Size: 187 6201
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2156 2264 2456 2732 3247 3528 3621
Size: 219 1111 108 192 276 515 281 93
Cuts at: 3621 3736 4871 5552 5743 826
Size: 115 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 515 281 276 219
192 191 115 108 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 2116 1700
Size: 416 5972
SmaI CCC'GGG
Cuts at: 1872 3474 3715 1872
Size: 1602 241 4545
Fragments arranged by size:
4545 1602 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 2516 3590 4492 5393 5655
Size: 197 926 351 222 1074 902 901 262
Cuts at: 5655 820
Size: 1553
Fragments arranged by size:
1553 1074 926 902 901 351 262 222
197
SnaBI TAC'GTA
Cuts at: 493 3685 4179 493
Size: 3192 494 2702
Fragments arranged by size:
3192 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3331 3746 1132
Size: 2199 415 3774
Fragments arranged by size:
3774 2199 415
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 3827 513
Size: 552 2762 3074
Fragments arranged by size:
3074 2762 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2633 3535 4415 4432 4585 4770 5215
Size: 1255 1053 902 880 17 153 185 445
Cuts at: 5215 325
Size: 1498
Fragments arranged by size:
1498 1255 1053 902 880 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3666 4634 6346
Size: 274 80 33 51 1443 1689 968 1712
Cuts at: 6346 96
Size: 138
Fragments arranged by size:
1712 1689 1443 968 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6348 6380 6348
Size: 32 6356
Tth111I GACn'n_nGTC
Cuts at: 1316 2409 1316
Size: 1093 5295
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 2711
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3472 3713 1870
Size: 1602 241 4545
Fragments arranged by size:
4545 1602 241
XmnI GAAnn'nnTTC
Cuts at: 4517 4517
Size: 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI