Restriction map for
pFN21ASDA1904
(Circular) (Six-base) MAPSORT of: pFN21ASDA1904.seq Check: 4468 from: 1 to: 7189
pFN21ASDA1904 7189 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3149 3149
Size: 7189
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3030 4999 278
Size: 53 83 186 936 1494 1969 2468
Fragments arranged by size:
2468 1969 1494 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4532 1937
Size: 2595 4594
Acc65I G'GTAC_C
Cuts at: 4510 4510
Size: 7189
AclI AA'CG_TT
Cuts at: 1563 5316 5689 1563
Size: 3753 373 3063
Fragments arranged by size:
3753 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5252 6636 6904 1632
Size: 3620 1384 268 1917
Fragments arranged by size:
3620 1917 1384 268
AfeI AGC'GCT
Cuts at: 4337 4337
Size: 7189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6992
AflIII A'CryG_T
Cuts at: 1203 2121 6088 7065 1203
Size: 918 3967 977 1327
Fragments arranged by size:
3967 1327 977 918
AgeI A'CCGG_T
Cuts at: 6020 6020
Size: 7189
AhdI GACnn_n'nnGTC
Cuts at: 5918 5918
Size: 7189
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2279
Size: 32 7157
AlwNI CAG_nnn'CTG
Cuts at: 2344 3397 3776 3794 4480 4932 6504 2344
Size: 1053 379 18 686 452 1572 3029
Fragments arranged by size:
3029 1572 1053 686 452 379 18
ApaI G_GGCC'C
Cuts at: 3808 3808
Size: 7189
ApaLI G'TGCA_C
Cuts at: 2192 3380 5246 6402 2192
Size: 1188 1866 1156 2979
Fragments arranged by size:
2979 1866 1188 1156
ApoI r'AATT_y
Cuts at: 1493 4498 4724 4876 6985 1493
Size: 3005 226 152 2109 1697
Fragments arranged by size:
3005 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5743 160
Size: 872 4711 1606
Fragments arranged by size:
4711 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
AvaI C'yCGr_G
Cuts at: 1870 1943 2473 2839 3572 3593 3737 3800 4298
Size: 73 530 366 733 21 144 63 498
Cuts at: 4298 4514 1870
Size: 216 4545
Fragments arranged by size:
4545 733 530 498 366 216 144 73
63 21
BamHI G'GATC_C
Cuts at: 4519 4886 4519
Size: 367 6822
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2312 2525 2704 3184
Size: 325 217 627 288 237 213 179 480
Cuts at: 3184 3545 4060 4181 4510 5965 618
Size: 361 515 121 329 1455 1842
Fragments arranged by size:
1842 1455 627 515 480 361 329 325
288 237 217 213 179 121
BanII G_rGCy'C
Cuts at: 1391 3611 3808 3851 4508 1391
Size: 2220 197 43 657 4072
Fragments arranged by size:
4072 2220 657 197 43
BbeI G_GCGC'C
Cuts at: 3188 3549 3188
Size: 361 6828
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2620 3335 928
Size: 971 721 715 4782
Fragments arranged by size:
4782 971 721 715
BbvCI CC'TCA_GC
Cuts at: 2510 3787 2510
Size: 1277 5912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2294 2328 3232 3266 3511 3545 4163
Size: 34 357 34 904 34 245 34 618
Cuts at: 4163 4197 5380 5414 1903
Size: 34 1183 34 3678
Fragments arranged by size:
3678 1183 904 618 357 245 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5081 6291 5081
Size: 1210 5979
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7189
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2779 3240 3887
Size: 107 122 71 631 605 1107 461 647
Cuts at: 3887 5800 136
Size: 1913 1525
Fragments arranged by size:
1913 1525 1107 647 631 605 461 122
107 71
BglII A'GATC_T
Cuts at: 2292 3621 4326 7184 2292
Size: 1329 705 2858 2297
Fragments arranged by size:
2858 2297 1329 705
BlpI GC'TnA_GC
Cuts at: 2366 3875 4606 2366
Size: 1509 731 4949
Fragments arranged by size:
4949 1509 731
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2474 2709 3384 3808 3966 5250 6406
Size: 406 278 235 675 424 158 1284 1156
Cuts at: 6406 1790
Size: 2573
Fragments arranged by size:
2573 1284 1156 675 424 406 278 235
158
BmgBI CAC'GTC
Cuts at: 1318 2932 3961 1318
Size: 1614 1029 4546
Fragments arranged by size:
4546 1614 1029
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4920 5878 442
Size: 416 53 83 399 24 3503 958 1753
Fragments arranged by size:
3503 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 3359 3830 4133 4276 5849 1239
Size: 1490 630 471 303 143 1573 2579
Fragments arranged by size:
2579 1573 1490 630 471 303 143
Bpu10I CC'TnA_GC
Cuts at: 2510 3787 3993 4193 2510
Size: 1277 206 200 5506
Fragments arranged by size:
5506 1277 206 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 3671 4199 5314 6179 6477 3023
Size: 648 528 1115 865 298 3735
Fragments arranged by size:
3735 1115 865 648 528 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 4468 5852 882
Size: 617 657 2312 1384 2219
Fragments arranged by size:
2312 2219 1384 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 4486 4980 493
Size: 711 918 2364 494 2702
Fragments arranged by size:
2702 2364 918 711 494
BsaBI GATnn'nnATC
Cuts at: 2990 7183 2990
Size: 4193 2996
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2450 2814 3027 3185
Size: 53 83 186 936 917 364 213 158
Cuts at: 3185 3546 4996 5378 6013 275
Size: 361 1450 382 635 1451
Fragments arranged by size:
1451 1450 936 917 635 382 364 361
213 186 158 83 53
BsaWI w'CCGG_w
Cuts at: 5622 6020 6294 6441 6852 5622
Size: 398 274 147 411 5959
Fragments arranged by size:
5959 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 2483 2513 4358
Size: 30 165 30 876 30 174 30 1845
Cuts at: 4358 4388 1178
Size: 30 3979
Fragments arranged by size:
3979 1845 876 174 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 3314 3775 4190 4279 4382 1186
Size: 1044 1084 461 415 89 103 3993
Fragments arranged by size:
3993 1084 1044 461 415 103 89
BseYI C'CCAG_C
Cuts at: 2350 3188 3256 3397 3818 4470 6392 6888 2350
Size: 838 68 141 421 652 1922 496 2651
Fragments arranged by size:
2651 1922 838 652 496 421 141 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 3020 1163
Size: 573 1173 111 5332
Fragments arranged by size:
5332 1173 573 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2072 2656 3891 3978 4639 5400
Size: 275 186 71 584 1235 87 661 761
Cuts at: 5400 6428 1540
Size: 1028 2301
Fragments arranged by size:
2301 1235 1028 761 661 584 275 186
87 71
BsiHKAI G_wGCw'C
Cuts at: 2196 2235 3384 3851 4146 4319 5250 5335 6406
Size: 39 1149 467 295 173 931 85 1071
Cuts at: 6406 2196
Size: 2979
Fragments arranged by size:
2979 1149 1071 931 467 295 173 85
39
BsmI GAATG_Cn'
Cuts at: 4700 4793 4700
Size: 93 7096
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2235 2474 2709 3384 3611 3808
Size: 399 406 39 239 235 675 227 197
Cuts at: 3808 3851 3966 4146 4319 4508 5250 5335 6406
Size: 43 115 180 173 189 742 85 1071
Cuts at: 6406 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 675 406 399 239 235
227 197 189 180 173 115 85 43
39
BspEI T'CCGG_A
Cuts at: 6852 6852
Size: 7189
BspHI T'CATG_A
Cuts at: 3030 3216 5078 6808 3030
Size: 186 1862 1730 3411
Fragments arranged by size:
3411 1862 1730 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 2329 3149 3914 4544 844
Size: 1364 121 820 765 630 3489
Fragments arranged by size:
3489 1364 820 765 630 121
BsrBI CCG'CTC
Cuts at: 1394 1823 3814 4230 5076 1394
Size: 429 1991 416 846 3507
Fragments arranged by size:
3507 1991 846 429 416
BsrDI GCAATG_nn'
Cuts at: 65 1248 3630 5684 5858 65
Size: 1183 2382 2054 174 1396
Fragments arranged by size:
2382 2054 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 2570 2687 2878 3368 3548 4047
Size: 110 257 503 117 191 490 180 499
Cuts at: 4047 4063 4123 4146 4385 5833 6020 1700
Size: 16 60 23 239 1448 187 2869
Fragments arranged by size:
2869 1448 503 499 490 257 239 191
187 180 117 110 60 23 16
BsrGI T'GTAC_A
Cuts at: 96 2988 4374 96
Size: 2892 1386 2911
Fragments arranged by size:
2911 2892 1386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7189
BssSI C'ACGA_G
Cuts at: 2996 5249 6261 2996
Size: 2253 1012 3924
Fragments arranged by size:
3924 2253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 2344 3776 3906 794
Size: 1406 144 1432 130 4077
Fragments arranged by size:
4077 1432 1406 144 130
BstBI TT'CG_AA
Cuts at: 2007 5993 2007
Size: 3986 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7189
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2956 3481 3643 3988 2554
Size: 402 525 162 345 5755
Fragments arranged by size:
5755 525 402 345 162
BstYI r'GATC_y
Cuts at: 1972 2292 2859 3621 4326 4429 4519 4886 5271
Size: 320 567 762 705 103 90 367 385
Cuts at: 5271 5288 6740 6826 6838 7184 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 762 705 567 385 367 346
320 103 90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2675 2892 513
Size: 552 1610 217 4810
Fragments arranged by size:
4810 1610 552 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6450
Fragments arranged by size:
6450 683 56
BtsI GCAGTG_nn'
Cuts at: 2669 3733 4132 4705 5499 5519 2669
Size: 1064 399 573 794 20 4339
Fragments arranged by size:
4339 1064 794 573 399 20
ClaI AT'CG_AT
Cuts at: 1554 3678 1554
Size: 2124 5065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7154
DraI TTT'AAA
Cuts at: 4493 4493
Size: 7189
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6196 809
Size: 507 4880 1802
Fragments arranged by size:
4880 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2130 2568
Size: 54 1425 50 275 18 239 61 438
Cuts at: 2568 2946 3601 3888 3975 4380 4636 5525 7154
Size: 378 655 287 87 405 256 889 1629
Cuts at: 7154 8
Size: 43
Fragments arranged by size:
1629 1425 889 655 438 405 378 287
275 256 239 87 61 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2069 3888 3975 4636 1537
Size: 275 257 1819 87 661 4090
Fragments arranged by size:
4090 1819 661 275 257 87
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 5119 1353
Size: 1253 2513 3423
Fragments arranged by size:
3423 2513 1253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4456 5776 6150 6296 387
Size: 1064 138 2867 1320 374 146 1280
Fragments arranged by size:
2867 1320 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3849 3849
Size: 7189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2729 3359 3830 4133 4276 5252 5849
Size: 393 1097 630 471 303 143 976 597
Cuts at: 5849 6636 6904 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1097 976 787 630 597 471 393
303 268 143
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2438 2378
Size: 60 7129
EcoO109I rG'GnC_Cy
Cuts at: 2341 3163 3733 3804 3805 2341
Size: 822 570 71 1 5725
Fragments arranged by size:
5725 822 570 71 1
EcoRI G'AATT_C
Cuts at: 4498 4876 4498
Size: 378 6811
FspI TGC'GCA
Cuts at: 3116 3281 3539 4400 5695 3116
Size: 165 258 861 1295 4610
Fragments arranged by size:
4610 1295 861 258 165
FspAI rTGC'GCAy
Cuts at: 3539 3539
Size: 7189
HaeII r_GCGC'y
Cuts at: 1678 2054 3188 3549 3871 3882 3942 4339 6336
Size: 376 1134 361 322 11 60 397 1997
Cuts at: 6336 1678
Size: 2531
Fragments arranged by size:
2531 1997 1134 397 376 361 322 60
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2822 2854 3356
Size: 32 135 32 911 32 307 32 502
Cuts at: 3356 3388 3686 3718 3743 3775 4292 4324 5830
Size: 32 298 32 25 32 517 32 1506
Cuts at: 5830 5862 5904 5936 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1506 911 517 502 307 298 135
42 32 32 32 32 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3319 3655 4533 4779 669
Size: 988 281 1381 336 878 246 3079
Fragments arranged by size:
3079 1381 988 878 336 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7189
HpaI GTT'AAC
Cuts at: 4779 4779
Size: 7189
KasI G'GCGC_C
Cuts at: 3184 3545 3184
Size: 361 6828
KpnI G_GTAC'C
Cuts at: 4514 4514
Size: 7189
MfeI C'AATT_G
Cuts at: 4788 4788
Size: 7189
MluI A'CGCG_T
Cuts at: 7065 7065
Size: 7189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2526 2738 3048 3462 6303 6487 1305
Size: 1221 212 310 414 2841 184 2007
Fragments arranged by size:
2841 2007 1221 414 310 212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 10
Size: 54 1425 343 300 5067
Fragments arranged by size:
5067 1425 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2954 2996 5147 5506 5665 518
Size: 648 1275 513 42 2151 359 159 2042
Fragments arranged by size:
2151 2042 1275 648 513 359 159 42
MspA1I CmG'CkG
Cuts at: 1606 2350 2677 2698 3773 3874 4042 4057 4074
Size: 744 327 21 1075 101 168 15 17
Cuts at: 4074 4086 4222 4605 5282 6430 6675 1606
Size: 12 136 383 677 1148 245 2120
Fragments arranged by size:
2120 1148 1075 744 677 383 327 245
168 136 101 21 17 15 12
NaeI GCC'GGC
Cuts at: 2069 2572 2689 2880 3370 3550 4065 4125 2069
Size: 503 117 191 490 180 515 60 5133
Fragments arranged by size:
5133 515 503 490 191 180 117 60
NarI GG'CG_CC
Cuts at: 3185 3546 3185
Size: 361 6828
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6637
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7189
NgoMIV G'CCGG_C
Cuts at: 2067 2570 2687 2878 3368 3548 4063 4123 2067
Size: 503 117 191 490 180 515 60 5133
Fragments arranged by size:
5133 515 503 490 191 180 117 60
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7189
NotI GC'GGCC_GC
Cuts at: 4636 4636
Size: 7189
NspI r_CATG'y
Cuts at: 1132 1229 2389 3295 4547 6092 1132
Size: 97 1160 906 1252 1545 2229
Fragments arranged by size:
2229 1545 1252 1160 906 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7189
PciI A'CATG_T
Cuts at: 6088 6088
Size: 7189
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3397 4111 1108
Size: 2289 714 4186
Fragments arranged by size:
4186 2289 714
PfoI T'CCnGG_A
Cuts at: 3336 3585 3336
Size: 249 6940
PmeI GTTT'AAAC
Cuts at: 4493 4493
Size: 7189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2311 5258 5290 6771 6803 2279
Size: 32 2947 32 1481 32 2665
Fragments arranged by size:
2947 2665 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 3163 3733 2341
Size: 822 570 5797
Fragments arranged by size:
5797 822 570
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7189
PsiI TTA'TAA
Cuts at: 4759 4759
Size: 7189
PspOMI G'GGCC_C
Cuts at: 3804 3804
Size: 7189
PstI C_TGCA'G
Cuts at: 830 2141 2340 2349 2412 2664 3619 3772 3911
Size: 1311 199 9 63 252 955 153 139
Cuts at: 3911 4139 4541 830
Size: 228 402 3478
Fragments arranged by size:
3478 1311 955 402 252 228 199 153
139 63 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7189
PvuII CAG'CTG
Cuts at: 2350 3773 3874 2350
Size: 1423 101 5665
Fragments arranged by size:
5665 1423 101
SacI G_AGCT'C
Cuts at: 3851 3851
Size: 7189
SacII CC_GC'GG
Cuts at: 2678 2678
Size: 7189
SalI G'TCGA_C
Cuts at: 1936 4531 1936
Size: 2595 4594
SanDI GG'GwC_CC
Cuts at: 3163 3733 3163
Size: 570 6619
SbfI CC_TGCA'GG
Cuts at: 2340 4541 2340
Size: 2201 4988
ScaI AGT'ACT
Cuts at: 4304 5437 7149 4304
Size: 1133 1712 4344
Fragments arranged by size:
4344 1712 1133
SfcI C'TryA_G
Cuts at: 826 1045 2137 2336 2345 2408 2660 2977 3615
Size: 219 1092 199 9 63 252 317 638
Cuts at: 3615 3768 3863 3907 4135 4537 5672 6353 6544
Size: 153 95 44 228 402 1135 681 191
Cuts at: 6544 826
Size: 1471
Fragments arranged by size:
1471 1135 1092 681 638 402 317 252
228 219 199 191 153 95 63 44
9
SfoI GGC'GCC
Cuts at: 3186 3547 3186
Size: 361 6828
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
SgrAI Cr'CCGG_yG
Cuts at: 1700 3548 1700
Size: 1848 5341
SmaI CCC'GGG
Cuts at: 1872 3802 4516 1872
Size: 1930 714 4545
Fragments arranged by size:
4545 1930 714
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3038 3593 3686 4214 5293 6194
Size: 197 926 1095 555 93 528 1079 901
Cuts at: 6194 6456 820
Size: 262 1553
Fragments arranged by size:
1553 1095 1079 926 901 555 528 262
197 93
SnaBI TAC'GTA
Cuts at: 493 4486 4980 493
Size: 3993 494 2702
Fragments arranged by size:
3993 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7189
SphI G_CATG'C
Cuts at: 1132 2389 3295 4547 1132
Size: 1257 906 1252 3774
Fragments arranged by size:
3774 1257 1252 906
SspI AAT'ATT
Cuts at: 5 52 5113 5
Size: 47 5061 2081
Fragments arranged by size:
5061 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7189
StyI C'CwwG_G
Cuts at: 513 1065 3474 3504 3636 3942 4419 4628 513
Size: 552 2409 30 132 306 477 209 3074
Fragments arranged by size:
3074 2409 552 477 306 209 132 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3221 3517 3873 5216 5233 5386 5571
Size: 1255 1641 296 356 1343 17 153 185
Cuts at: 5571 6016 325
Size: 445 1498
Fragments arranged by size:
1641 1498 1343 1255 445 356 296 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2988 3003 3090
Size: 274 80 33 51 1443 1011 15 87
Cuts at: 3090 3267 4008 4167 4302 4374 5435 7147 96
Size: 177 741 159 135 72 1061 1712 138
Fragments arranged by size:
1712 1443 1061 1011 741 274 177 159
138 135 87 80 72 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7149 7181 7149
Size: 32 7157
Tth111I GACn'n_nGTC
Cuts at: 1316 3167 3650 1316
Size: 1851 483 4855
Fragments arranged by size:
4855 1851 483
XbaI T'CTAG_A
Cuts at: 4525 4525
Size: 7189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 7189
XhoI C'TCGA_G
Cuts at: 1943 3593 1943
Size: 1650 5539
XmaI C'CCGG_G
Cuts at: 1870 3800 4514 1870
Size: 1930 714 4545
Fragments arranged by size:
4545 1930 714
XmnI GAAnn'nnTTC
Cuts at: 5318 5318
Size: 7189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3028 4997 276
Size: 53 83 186 936 1494 1969 2468
Fragments arranged by size:
2468 1969 1494 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SexAI SfiI SrfI
SwaI