Restriction map for
pFN21ASDA1896
(Circular) (Six-base) MAPSORT of: pFN21ASDA1896.seq Check: 2769 from: 1 to: 6232
pFN21ASDA1896 6232 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3186 3486 3575 1937
Size: 1249 300 89 4594
Fragments arranged by size:
4594 1249 300 89
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3050 3518 4295 5679 5947 1632
Size: 1418 468 777 1384 268 1917
Fragments arranged by size:
1917 1418 1384 777 468 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2784 3237 1184
Size: 1600 453 4179
Fragments arranged by size:
4179 1600 453
AlwNI CAG_nnn'CTG
Cuts at: 2035 2126 2401 2724 3285 3975 5547 2035
Size: 91 275 323 561 690 1572 2720
Fragments arranged by size:
2720 1572 690 561 323 275 91
ApaI G_GGCC'C
Cuts at: 2483 2483
Size: 6232
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2294 2445 2897 3541 3767 3919 6028 1493
Size: 801 151 452 644 226 152 2109 1697
Fragments arranged by size:
2109 1697 801 644 452 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2573 3557 1870
Size: 73 630 984 4545
Fragments arranged by size:
4545 984 630 73
BamHI G'GATC_C
Cuts at: 3093 3562 3929 3093
Size: 469 367 5396
Fragments arranged by size:
5396 469 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2114 3269 3553 5008
Size: 325 217 627 286 41 1155 284 1455
Cuts at: 5008 618
Size: 1842
Fragments arranged by size:
1842 1455 1155 627 325 286 284 217
41
BanII G_rGCy'C
Cuts at: 1391 2483 3551 1391
Size: 1092 1068 4072
Fragments arranged by size:
4072 1092 1068
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3038 3059 928
Size: 971 1139 21 4101
Fragments arranged by size:
4101 1139 971 21
BbvCI CC'TCA_GC
Cuts at: 2178 2330 2178
Size: 152 6080
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2725 4124 5334 2725
Size: 1399 1210 3623
Fragments arranged by size:
3623 1399 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2483 4293 5449 1790
Size: 693 1810 1156 2573
Fragments arranged by size:
2573 1810 1156 693
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2055 1056
Size: 999 5233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2152 2277 4892 1239
Size: 829 84 125 2615 2579
Fragments arranged by size:
2615 2579 829 125 84
Bpu10I CC'TnA_GC
Cuts at: 2178 2330 2178
Size: 152 6080
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2732 3182 4357 5222 5520 2435
Size: 297 450 1175 865 298 3147
Fragments arranged by size:
3147 1175 865 450 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2130 4895 882
Size: 617 631 2765 2219
Fragments arranged by size:
2765 2219 631 617
BsaAI yAC'GTr
Cuts at: 493 1204 3481 3529 4023 493
Size: 711 2277 48 494 2702
Fragments arranged by size:
2702 2277 711 494 48
BsaBI GATnn'nnATC
Cuts at: 2516 6226 2516
Size: 3710 2522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3361 4665 5063 5337 5484 5895 3361
Size: 1304 398 274 147 411 3698
Fragments arranged by size:
3698 1304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 2669 1186
Size: 1354 129 4749
Fragments arranged by size:
4749 1354 129
BseYI C'CCAG_C
Cuts at: 2069 2119 3148 3460 5435 5931 2069
Size: 50 1029 312 1975 496 2370
Fragments arranged by size:
2370 1975 1029 496 312 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2710 3536 1163
Size: 573 974 826 3859
Fragments arranged by size:
3859 974 826 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 3682 4443 5471 1540
Size: 275 186 100 1581 761 1028 2301
Fragments arranged by size:
2301 1581 1028 761 275 186 100
BsiHKAI G_wGCw'C
Cuts at: 2757 3258 4293 4378 5449 2757
Size: 501 1035 85 1071 3540
Fragments arranged by size:
3540 1071 1035 501 85
BsmI GAATG_Cn'
Cuts at: 2596 3078 3208 3743 3836 2596
Size: 482 130 535 93 4992
Fragments arranged by size:
4992 535 482 130 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3185 3185
Size: 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2483 2757 3258 3551 4293 4378 5449
Size: 399 693 274 501 293 742 85 1071
Cuts at: 5449 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 693 501 399 293 274
85
BspEI T'CCGG_A
Cuts at: 3361 5895 3361
Size: 2534 3698
BspHI T'CATG_A
Cuts at: 2317 3447 4121 5851 2317
Size: 1130 674 1730 2698
Fragments arranged by size:
2698 1730 1130 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 2732 2752 3587 844
Size: 1386 502 20 835 3489
Fragments arranged by size:
3489 1386 835 502 20
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 4727 4901 65
Size: 1183 1612 1867 174 1396
Fragments arranged by size:
1867 1612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3167 4876 5063 1700
Size: 110 1357 1709 187 2869
Fragments arranged by size:
2869 1709 1357 187 110
BsrGI T'GTAC_A
Cuts at: 96 2248 3423 96
Size: 2152 1175 2905
Fragments arranged by size:
2905 2152 1175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 2960 4292 5304 2960
Size: 1332 1012 3888
Fragments arranged by size:
3888 1332 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 794
Size: 1607 4625
BstBI TT'CG_AA
Cuts at: 2007 5036 2007
Size: 3029 3203
BstEII G'GTnAC_C
Cuts at: 1184 3367 1184
Size: 2183 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 3148 2209
Size: 939 5293
BstYI r'GATC_y
Cuts at: 1972 3093 3562 3929 4314 4331 5783 5869 5881
Size: 1121 469 367 385 17 1452 86 12
Cuts at: 5881 6227 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1121 469 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2077 2184 2559 513
Size: 552 1012 107 375 4186
Fragments arranged by size:
4186 1012 552 375 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2980 487
Size: 683 56 1754 3739
Fragments arranged by size:
3739 1754 683 56
BtsI GCAGTG_nn'
Cuts at: 3231 3748 4542 4562 3231
Size: 517 794 20 4901
Fragments arranged by size:
4901 794 517 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2590 2625 529
Size: 35 2026 35 4136
Fragments arranged by size:
4136 2026 35 35
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DraIII CAC_nnn'GTG
Cuts at: 2560 2689 2560
Size: 129 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2414 3182 5239 809
Size: 507 1098 768 2057 1802
Fragments arranged by size:
2057 1802 1098 768 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3099 3114 3289
Size: 54 1425 50 275 18 1269 15 175
Cuts at: 3289 3297 3679 4568 6197 8
Size: 8 382 889 1629 43
Fragments arranged by size:
1629 1425 1269 889 382 275 175 54
50 43 18 15 8
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3389 4162 1353
Size: 2036 773 3423
Fragments arranged by size:
3423 2036 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2152 2277 3050 3518 4295 4892
Size: 393 436 84 125 773 468 777 597
Cuts at: 4892 5679 5947 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 777 773 597 468 436 393
268 125 84
EcoO109I rG'GnC_Cy
Cuts at: 2266 2479 2480 2979 3025 3324 3411 2266
Size: 213 1 499 46 299 87 5087
Fragments arranged by size:
5087 499 299 213 87 46 1
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
EcoRV GAT'ATC
Cuts at: 2424 2424
Size: 6232
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2926 3047 3360 5379 1678
Size: 1248 121 313 2019 2531
Fragments arranged by size:
2531 2019 1248 313 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4873 4905 4947 4979 1373
Size: 32 135 32 3301 32 42 32 2626
Fragments arranged by size:
3301 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3822 669
Size: 988 281 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2149 748
Size: 1401 4831
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2197 5346 5530 1305
Size: 892 3149 184 2007
Fragments arranged by size:
3149 2007 892 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3101 3291 3299 10
Size: 54 1425 343 1269 190 8 2943
Fragments arranged by size:
2943 1425 1269 343 190 54 8
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2813 4190 4549 4708 518
Size: 648 1326 321 1377 359 159 2042
Fragments arranged by size:
2042 1377 1326 648 359 321 159
MspA1I CmG'CkG
Cuts at: 1606 3295 3464 3648 4325 5473 5718 1606
Size: 1689 169 184 677 1148 245 2120
Fragments arranged by size:
2120 1689 1148 677 245 184 169
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 6232
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5680
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 2051 1052
Size: 999 5233
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3148 3590 5135 1132
Size: 97 1919 442 1545 2229
Fragments arranged by size:
2229 1919 1545 442 97
PasI CC'CwG_GG
Cuts at: 1348 2064 3508 1348
Size: 716 1444 4072
Fragments arranged by size:
4072 1444 716
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2523 2560 1108
Size: 1415 37 4780
Fragments arranged by size:
4780 1415 37
PfoI T'CCnGG_A
Cuts at: 2634 2634
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2979 3025 3324 2266
Size: 713 46 299 5174
Fragments arranged by size:
5174 713 299 46
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 2479 2479
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2227 3216 3584 830
Size: 1397 989 368 3478
Fragments arranged by size:
3478 1397 989 368
PvuI CG_AT'CG
Cuts at: 2001 2101 2001
Size: 100 6132
PvuII CAG'CTG
Cuts at: 3295 3464 3295
Size: 169 6063
RsrII CG'GwC_CG
Cuts at: 3402 3402
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 3065 4480 6192 3065
Size: 1415 1712 3105
Fragments arranged by size:
3105 1712 1415
SexAI A'CCwGG_T
Cuts at: 2685 2685
Size: 6232
SfcI C'TryA_G
Cuts at: 826 1045 2223 3212 3580 4715 5396 5587 826
Size: 219 1178 989 368 1135 681 191 1471
Fragments arranged by size:
1471 1178 1135 989 681 368 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 2747 3197 4336 5237 5499
Size: 197 926 507 297 450 1139 901 262
Cuts at: 5499 820
Size: 1553
Fragments arranged by size:
1553 1139 926 901 507 450 297 262
197
SnaBI TAC'GTA
Cuts at: 493 3529 4023 493
Size: 3036 494 2702
Fragments arranged by size:
3036 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2982 3671 513
Size: 552 1917 689 3074
Fragments arranged by size:
3074 1917 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 2606 3419 4259 4276 4429 4614
Size: 1255 481 545 813 840 17 153 185
Cuts at: 4614 5059 325
Size: 445 1498
Fragments arranged by size:
1498 1255 840 813 545 481 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 3063 3423
Size: 274 80 33 51 1443 271 815 360
Cuts at: 3423 4478 6190 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 815 360 274 271 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2127 2206 2209 2899 3094 3109 3307 2127
Size: 79 3 690 195 15 198 5052
Fragments arranged by size:
5052 690 198 195 79 15 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 2445 4361 2445
Size: 1916 4316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI