Restriction map for
pFN21ASDA1895
(Circular) (Six-base) MAPSORT of: pFN21ASDA1895.seq Check: 7361 from: 1 to: 6562
pFN21ASDA1895 6562 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3143 3143
Size: 6562
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 3905 1937
Size: 1629 339 4594
Fragments arranged by size:
4594 1629 339
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 3020 4689 5062 1563
Size: 1457 1669 373 3063
Fragments arranged by size:
3063 1669 1457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 3377 4625 6009 6277 1632
Size: 855 890 1248 1384 268 1917
Fragments arranged by size:
1917 1384 1248 890 855 268
AflII C'TTAA_G
Cuts at: 820 1017 2303 820
Size: 197 1286 5079
Fragments arranged by size:
5079 1286 197
AflIII A'CryG_T
Cuts at: 1203 3520 5461 6438 1203
Size: 2317 1941 977 1327
Fragments arranged by size:
2317 1941 1327 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 2891 1166
Size: 1725 4837
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3486 1184
Size: 2302 4260
AlwNI CAG_nnn'CTG
Cuts at: 4305 5877 4305
Size: 1572 4990
ApaLI G'TGCA_C
Cuts at: 4619 5775 4619
Size: 1156 5406
ApoI r'AATT_y
Cuts at: 1493 2570 3040 3626 3871 4097 4249 6358 1493
Size: 1077 470 586 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 1077 586 470 245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 3887 1870
Size: 73 1944 4545
Fragments arranged by size:
4545 1944 73
BamHI G'GATC_C
Cuts at: 3892 4259 3892
Size: 367 6195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3883 5338 618
Size: 325 217 627 2096 1455 1842
Fragments arranged by size:
2096 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3881 1391
Size: 2490 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 928
Size: 971 333 5258
Fragments arranged by size:
5258 971 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4753 4787 1903
Size: 34 2816 34 3678
Fragments arranged by size:
3678 2816 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6562
BfrBI ATG'CAT
Cuts at: 3688 3688
Size: 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5173 136
Size: 107 122 71 631 605 3501 1525
Fragments arranged by size:
3501 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6557 6557
Size: 6562
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 4623 5779 1790
Size: 2833 1156 2573
Fragments arranged by size:
2833 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2838 2919 4293
Size: 416 53 83 399 24 1421 81 1374
Cuts at: 4293 5251 442
Size: 958 1753
Fragments arranged by size:
1753 1421 1374 958 416 399 83 81
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3763 3763
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2747 5222 1239
Size: 1100 408 2475 2579
Fragments arranged by size:
2579 2475 1100 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4687 5552 5850 4687
Size: 865 298 5399
Fragments arranged by size:
5399 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 2157 5225 882
Size: 617 611 47 3068 2219
Fragments arranged by size:
3068 2219 617 611 47
BsaAI yAC'GTr
Cuts at: 493 1204 3859 4353 493
Size: 711 2655 494 2702
Fragments arranged by size:
2702 2655 711 494
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4369 4751 5386 275
Size: 53 83 186 936 2836 382 635 1451
Fragments arranged by size:
2836 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2101 4995 5393 5667 5814 6225 2101
Size: 2894 398 274 147 411 2438
Fragments arranged by size:
2894 2438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6337
Fragments arranged by size:
6337 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 1186
Size: 989 5573
BseYI C'CCAG_C
Cuts at: 2781 5765 6261 2781
Size: 2984 496 3082
Fragments arranged by size:
3082 2984 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2868 1163
Size: 573 1132 4857
Fragments arranged by size:
4857 1132 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4012 4773 5801 1540
Size: 275 186 2011 761 1028 2301
Fragments arranged by size:
2301 2011 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 4623 4708 5779 2091
Size: 2532 85 1071 2874
Fragments arranged by size:
2874 2532 1071 85
BsmI GAATG_Cn'
Cuts at: 2451 4073 4166 2451
Size: 1622 93 4847
Fragments arranged by size:
4847 1622 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3083 3192 3083
Size: 109 6453
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 3881 4623 4708 5779 1391
Size: 399 301 1790 742 85 1071 2174
Fragments arranged by size:
2174 1790 1071 742 399 301 85
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 4451 6181 4451
Size: 1730 4832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 3917 844
Size: 2299 774 3489
Fragments arranged by size:
3489 2299 774
BsrBI CCG'CTC
Cuts at: 1394 1823 4449 1394
Size: 429 2626 3507
Fragments arranged by size:
3507 2626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5057 5231 65
Size: 1183 3809 174 1396
Fragments arranged by size:
3809 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5206 5393 1700
Size: 110 3396 187 2869
Fragments arranged by size:
3396 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6562
BstBI TT'CG_AA
Cuts at: 2007 5366 2007
Size: 3359 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 2534 3219 3449 2534
Size: 685 230 5647
Fragments arranged by size:
5647 685 230
BstYI r'GATC_y
Cuts at: 1972 3892 4259 4644 4661 6113 6199 6211 6557
Size: 1920 367 385 17 1452 86 12 346
Cuts at: 6557 1972
Size: 1977
Fragments arranged by size:
1977 1920 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3567 3567
Size: 6562
Bsu36I CC'TnA_GG
Cuts at: 3489 3489
Size: 6562
BtgI C'CryG_G
Cuts at: 513 1065 3753 3794 513
Size: 552 2688 41 3281
Fragments arranged by size:
3281 2688 552 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 4078 4872 4892 4078
Size: 794 20 5748
Fragments arranged by size:
5748 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3121 3156 529
Size: 35 2557 35 3935
Fragments arranged by size:
3935 2557 35 35
DraI TTT'AAA
Cuts at: 3148 3866 3148
Size: 718 5844
DraIII CAC_nnn'GTG
Cuts at: 2318 3419 2318
Size: 1101 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3344 5569 809
Size: 507 2028 2225 1802
Fragments arranged by size:
2225 2028 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2473 3439 3681
Size: 54 1425 50 275 18 643 966 242
Cuts at: 3681 4009 4898 6527 8
Size: 328 889 1629 43
Fragments arranged by size:
1629 1425 966 889 643 328 275 242
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4009 1537
Size: 275 2197 4090
Fragments arranged by size:
4090 2197 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3261 3324 4492 1353
Size: 1908 63 1168 3423
Fragments arranged by size:
3423 1908 1168 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5149 5523 5669 387
Size: 1064 138 3560 374 146 1280
Fragments arranged by size:
3560 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2487 2747 3377 4625 5222 6009
Size: 393 707 148 260 630 1248 597 787
Cuts at: 6009 6277 1239
Size: 268 1524
Fragments arranged by size:
1524 1248 787 707 630 597 393 268
260 148
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2417 2417
Size: 6562
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 5709 1678
Size: 4031 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2365 2397 2537 2569 2667
Size: 32 135 32 793 32 140 32 98
Cuts at: 2667 2699 2892 2924 5203 5235 5277 5309 1373
Size: 32 193 32 2279 32 42 32 2626
Fragments arranged by size:
2626 2279 793 193 140 135 98 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3906 4152 669
Size: 988 281 1968 246 3079
Fragments arranged by size:
3079 1968 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2630 748
Size: 1882 4680
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 2586 4161 2586
Size: 1575 4987
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5676 5860 1305
Size: 4371 184 2007
Fragments arranged by size:
4371 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2475 3441 3683 10
Size: 54 1425 343 643 966 242 2889
Fragments arranged by size:
2889 1425 966 643 343 242 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2891 3447 4520 4879 5038 518
Size: 648 1725 556 1073 359 159 2042
Fragments arranged by size:
2042 1725 1073 648 556 359 159
MspA1I CmG'CkG
Cuts at: 1606 3978 4655 5803 6048 1606
Size: 2372 677 1148 245 2120
Fragments arranged by size:
2372 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3753 3794 513
Size: 552 2688 41 3281
Fragments arranged by size:
3281 2688 552 41
NdeI CA'TA_TG
Cuts at: 387 3448 3561 387
Size: 3061 113 3388
Fragments arranged by size:
3388 3061 113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NsiI A_TGCA'T
Cuts at: 3690 3690
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 3179 3692 3920 5465 1132
Size: 97 1950 513 228 1545 2229
Fragments arranged by size:
2229 1950 1545 513 228 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6562
PciI A'CATG_T
Cuts at: 5461 5461
Size: 6562
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3419 1108
Size: 2311 4251
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PstI C_TGCA'G
Cuts at: 830 3914 830
Size: 3084 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SapI GCTCTTCn'nnn_
Cuts at: 3261 3324 3261
Size: 63 6499
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 2134 4810 6522 2134
Size: 2676 1712 2174
Fragments arranged by size:
2676 2174 1712
SfcI C'TryA_G
Cuts at: 826 1045 3910 5045 5726 5917 826
Size: 219 2865 1135 681 191 1471
Fragments arranged by size:
2865 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 4666 5567 5829 820
Size: 197 926 360 2363 901 262 1553
Fragments arranged by size:
2363 1553 926 901 360 262 197
SnaBI TAC'GTA
Cuts at: 493 3859 4353 493
Size: 3366 494 2702
Fragments arranged by size:
3366 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3692 3920 1132
Size: 2560 228 3774
Fragments arranged by size:
3774 2560 228
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6562
StyI C'CwwG_G
Cuts at: 513 1065 2386 2440 3308 3753 3794 4001 513
Size: 552 1321 54 868 445 41 207 3074
Fragments arranged by size:
3074 1321 868 552 445 207 54 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4589 4606 4759 4944 5389 325
Size: 1255 1215 1794 17 153 185 445 1498
Fragments arranged by size:
1794 1498 1255 1215 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2132 2430 4808
Size: 274 80 33 51 1443 155 298 2378
Cuts at: 4808 6520 96
Size: 1712 138
Fragments arranged by size:
2378 1712 1443 298 274 155 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 6522 6554 2681
Size: 32 3809 32 2689
Fragments arranged by size:
3809 2689 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 2156 3898 2156
Size: 1742 4820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6562
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 3522 4691 3522
Size: 1169 5393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI