Restriction map for
pFN21ASDA1892
(Circular) (Six-base) MAPSORT of: pFN21ASDA1892.seq Check: 8264 from: 1 to: 6088
pFN21ASDA1892 6088 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2163 3431 1937
Size: 226 1268 4594
Fragments arranged by size:
4594 1268 226
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 3330 4151 5535 5803 1632
Size: 647 1051 821 1384 268 1917
Fragments arranged by size:
1917 1384 1051 821 647 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2247 1184
Size: 1063 5025
AlwNI CAG_nnn'CTG
Cuts at: 2621 3831 5403 2621
Size: 1210 1572 3306
Fragments arranged by size:
3306 1572 1210
ApaI G_GGCC'C
Cuts at: 2096 2096
Size: 6088
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 3413 1870
Size: 73 271 1199 4545
Fragments arranged by size:
4545 1199 271 73
BamHI G'GATC_C
Cuts at: 2542 2989 3418 3785 2542
Size: 447 429 367 4845
Fragments arranged by size:
4845 447 429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2794 2966 3104 3130 3354 3375 3407
Size: 705 698 172 138 26 224 21 32
Cuts at: 3407 1391
Size: 4072
Fragments arranged by size:
4072 705 698 224 172 138 32 26
21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 928
Size: 971 867 4250
Fragments arranged by size:
4250 971 867
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2459 3980 5190 2459
Size: 1521 1210 3357
Fragments arranged by size:
3357 1521 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2096 4149 5305 1790
Size: 306 2053 1156 2573
Fragments arranged by size:
2573 2053 1156 306
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 2831 3819
Size: 416 53 83 399 24 827 587 988
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 988 958 827 587 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2489 1056
Size: 1433 4655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 3169 3356 2103
Size: 1066 187 4835
Fragments arranged by size:
4835 1066 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2514 3262 3397 4748 1239
Size: 1203 72 748 135 1351 2579
Fragments arranged by size:
2579 1351 1203 748 135 72
Bpu10I CC'TnA_GC
Cuts at: 2088 2088
Size: 6088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2630 3385 3879 493
Size: 711 1426 755 494 2702
Fragments arranged by size:
2702 1426 755 711 494
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3051 4521 4919 5193 5340 5751 3051
Size: 1470 398 274 147 411 3388
Fragments arranged by size:
3388 1470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 1178
Size: 30 165 30 852 30 4981
Fragments arranged by size:
4981 852 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2511 2876 1186
Size: 1325 365 4398
Fragments arranged by size:
4398 1325 365
BseYI C'CCAG_C
Cuts at: 2075 2106 2433 3368 5291 5787 2075
Size: 31 327 935 1923 496 2376
Fragments arranged by size:
2376 1923 935 496 327 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3075 3130 4149 4234 5305 3075
Size: 55 1019 85 1071 3858
Fragments arranged by size:
3858 1071 1019 85 55
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2794 2966 3075 3104 3130 3354
Size: 399 306 698 172 109 29 26 224
Cuts at: 3354 3375 3407 4149 4234 5305 1391
Size: 21 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 698 399 306 224 172
109 85 32 29 26 21
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3229 3977 5707 3229
Size: 748 1730 3610
Fragments arranged by size:
3610 1730 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2346 794
Size: 1552 4536
BstBI TT'CG_AA
Cuts at: 2007 4892 2007
Size: 2885 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2153 2394 2542 2580 2989 3418 3785 4170
Size: 181 241 148 38 409 429 367 385
Cuts at: 4170 4187 5639 5725 5737 6083 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 429 409 385 367 346 241
181 148 86 38 17 12
BstZ17I GTA'TAC
Cuts at: 2164 2164
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2022 2993 3355 513
Size: 552 957 971 362 3246
Fragments arranged by size:
3246 971 957 552 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2575 487
Size: 683 56 1349 4000
Fragments arranged by size:
4000 1349 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 2622 2835 2964 3049 3292 3604 4398 4418
Size: 142 213 129 85 243 312 794 20
Cuts at: 4418 2480
Size: 4150
Fragments arranged by size:
4150 794 312 243 213 142 129 85
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 2698 2698
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3138 4018 1353
Size: 791 994 880 3423
Fragments arranged by size:
3423 994 880 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3128 3128
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2442 2514 3262 3330 3397 4151
Size: 393 647 163 72 748 68 67 754
Cuts at: 4151 4748 5535 5803 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 754 748 647 597 393 268
163 72 68 67
EcoO109I rG'GnC_Cy
Cuts at: 2092 2283 2707 3335 2092
Size: 191 424 628 4845
Fragments arranged by size:
4845 628 424 191
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2060 5235 1678
Size: 382 3175 2531
Fragments arranged by size:
3175 2531 382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4729 4761 4803 4835 1373
Size: 32 135 32 3157 32 42 32 2626
Fragments arranged by size:
3157 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2229 2308 3083 4046 4405 4564 518
Size: 648 1063 79 775 963 359 159 2042
Fragments arranged by size:
2042 1063 963 775 648 359 159 79
MspA1I CmG'CkG
Cuts at: 1606 3504 4181 5329 5574 1606
Size: 1898 677 1148 245 2120
Fragments arranged by size:
2120 1898 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3355 513
Size: 552 957 1333 3246
Fragments arranged by size:
3246 1333 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 2485 1052
Size: 1433 4655
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3446 4991 1132
Size: 97 2217 1545 2229
Fragments arranged by size:
2229 2217 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2595 1108
Size: 1487 4601
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 2630 2630
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 2707 3335 2283
Size: 424 628 5036
Fragments arranged by size:
5036 628 424
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2092 2092
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SacI G_AGCT'C
Cuts at: 3130 3130
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SanDI GG'GwC_CC
Cuts at: 2283 2707 2283
Size: 424 5664
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2192 3436 4571 5252 5443 826
Size: 219 1147 1244 1135 681 191 1471
Fragments arranged by size:
1471 1244 1147 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2214 4192 5093 5355 820
Size: 197 926 271 1978 901 262 1553
Fragments arranged by size:
1978 1553 926 901 271 262 197
SnaBI TAC'GTA
Cuts at: 493 3385 3879 493
Size: 2892 494 2702
Fragments arranged by size:
2892 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 2738 1517
Size: 1221 4867
StyI C'CwwG_G
Cuts at: 513 1065 2022 2278 3355 3527 513
Size: 552 957 256 1077 172 3074
Fragments arranged by size:
3074 1077 957 552 256 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3305 4115 4132 4285 4470 4915 325
Size: 1255 1725 810 17 153 185 445 1498
Fragments arranged by size:
1725 1498 1255 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 6048 6080 2534
Size: 32 3482 32 2542
Fragments arranged by size:
3482 2542 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 2474 3424 2474
Size: 950 5138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2441 2441
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 2214 1943
Size: 271 5817
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspXI PsrI PvuII RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI