(Circular) (Six-base) MAPSORT of: pFN21ASDA1882.seq Check: 8521 from: 1 to: 8173 pFN21ASDA1882 8173 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4746 5061 4746 Size: 315 7858 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5983 278 Size: 53 83 186 936 4447 2468 Fragments arranged by size: 4447 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3426 5516 1937 Size: 1489 2090 4594 Fragments arranged by size: 4594 2090 1489 Acc65I G'GTAC_C Cuts at: 5494 5494 Size: 8173 AclI AA'CG_TT Cuts at: 1563 6300 6673 1563 Size: 4737 373 3063 Fragments arranged by size: 4737 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2101 2877 3003 3110 4650 5015 6236 7620 Size: 469 776 126 107 1540 365 1221 1384 Cuts at: 7620 7888 1632 Size: 268 1917 Fragments arranged by size: 1917 1540 1384 1221 776 469 365 268 126 107 AfeI AGC'GCT Cuts at: 3182 3182 Size: 8173 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7976 AflIII A'CryG_T Cuts at: 1203 2703 3079 3714 5442 7072 8049 1203 Size: 1500 376 635 1728 1630 977 1327 Fragments arranged by size: 1728 1630 1500 1327 977 635 376 AgeI A'CCGG_T Cuts at: 3613 7004 3613 Size: 3391 4782 AhdI GACnn_n'nnGTC Cuts at: 3505 6902 3505 Size: 3397 4776 AleI CACnn'nnGTG Cuts at: 1166 2414 3762 5445 1166 Size: 1248 1348 1683 3894 Fragments arranged by size: 3894 1683 1348 1248 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4145 5423 5463 1184 Size: 2961 1278 40 3894 Fragments arranged by size: 3894 2961 1278 40 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3539 3571 3539 Size: 32 8141 AlwNI CAG_nnn'CTG Cuts at: 2068 2780 3387 4038 5916 7488 2068 Size: 712 607 651 1878 1572 2753 Fragments arranged by size: 2753 1878 1572 712 651 607 ApaI G_GGCC'C Cuts at: 5193 5338 5193 Size: 145 8028 ApaLI G'TGCA_C Cuts at: 5237 5438 6230 7386 5237 Size: 201 792 1156 6024 Fragments arranged by size: 6024 1156 792 201 ApoI r'AATT_y Cuts at: 1493 3119 3174 4597 5482 5708 5860 7969 1493 Size: 1626 55 1423 885 226 152 2109 1697 Fragments arranged by size: 2109 1697 1626 1423 885 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 6727 160 Size: 872 5695 1606 Fragments arranged by size: 5695 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8173 AvaI C'yCGr_G Cuts at: 1870 1943 2210 4512 4541 4618 4695 4941 5330 Size: 73 267 2302 29 77 77 246 389 Cuts at: 5330 5498 1870 Size: 168 4545 Fragments arranged by size: 4545 2302 389 267 246 168 77 77 73 29 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4170 4203 4170 Size: 33 8140 BamHI G'GATC_C Cuts at: 3352 5503 5870 3352 Size: 2151 367 5655 Fragments arranged by size: 5655 2151 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2622 2909 3616 5062 Size: 325 217 627 409 426 287 707 1446 Cuts at: 5062 5494 6949 618 Size: 432 1455 1842 Fragments arranged by size: 1842 1455 1446 707 627 432 426 409 325 287 217 BanII G_rGCy'C Cuts at: 1391 2827 3204 3582 5177 5193 5279 5338 5492 Size: 1436 377 378 1595 16 86 59 154 Cuts at: 5492 1391 Size: 4072 Fragments arranged by size: 4072 1595 1436 378 377 154 86 59 16 BbeI G_GCGC'C Cuts at: 5066 5066 Size: 8173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 2991 4012 4583 4744 928 Size: 971 466 626 1021 571 161 4357 Fragments arranged by size: 4357 1021 971 626 571 466 161 BbvCI CC'TCA_GC Cuts at: 2052 2052 Size: 8173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4487 4521 6364 6398 1903 Size: 34 2550 34 1843 34 3678 Fragments arranged by size: 3678 2550 1843 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6065 7275 6065 Size: 1210 6963 BclI T'GATC_A Cuts at: 1548 1548 Size: 8173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5133 5456 6784 Size: 107 122 71 631 605 3461 323 1328 Cuts at: 6784 136 Size: 1525 Fragments arranged by size: 3461 1525 1328 631 605 323 122 107 71 BglII A'GATC_T Cuts at: 2334 4136 8168 2334 Size: 1802 4032 2339 Fragments arranged by size: 4032 2339 1802 BlpI GC'TnA_GC Cuts at: 5590 5590 Size: 8173 Bme1580I G_kGCm'C Cuts at: 1790 2887 2914 3621 5193 5241 5338 5442 5452 Size: 1097 27 707 1572 48 97 104 10 Cuts at: 5452 6234 7390 1790 Size: 782 1156 2573 Fragments arranged by size: 2573 1572 1156 1097 782 707 104 97 48 27 10 BmgBI CAC'GTC Cuts at: 1318 2416 2960 1318 Size: 1098 544 6531 Fragments arranged by size: 6531 1098 544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3947 4345 5427 Size: 416 53 83 399 24 2530 398 1082 Cuts at: 5427 5904 6862 442 Size: 477 958 1753 Fragments arranged by size: 2530 1753 1082 958 477 416 399 398 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3675 1056 Size: 2619 5554 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2841 4294 2841 Size: 1453 6720 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 4289 4632 4940 4970 4999 5066 5171 Size: 1280 1770 343 308 30 29 67 105 Cuts at: 5171 5263 5474 6833 1239 Size: 92 211 1359 2579 Fragments arranged by size: 2579 1770 1359 1280 343 308 211 105 92 67 30 29 Bpu10I CC'TnA_GC Cuts at: 2052 2975 2052 Size: 923 7250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 6298 7163 7461 4342 Size: 1956 865 298 5054 Fragments arranged by size: 5054 1956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6836 882 Size: 617 5337 2219 Fragments arranged by size: 5337 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2323 2647 3080 4573 5443 5470 5964 Size: 711 1119 324 433 1493 870 27 494 Cuts at: 5964 493 Size: 2702 Fragments arranged by size: 2702 1493 1119 870 711 494 433 324 27 BsaBI GATnn'nnATC Cuts at: 8167 8167 Size: 8173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5063 5980 6362 6997 Size: 53 83 186 936 3530 917 382 635 Cuts at: 6997 275 Size: 1451 Fragments arranged by size: 3530 1451 936 917 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3613 5169 5376 6606 7004 7278 7425 7836 3613 Size: 1556 207 1230 398 274 147 411 3950 Fragments arranged by size: 3950 1556 1230 411 398 274 207 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3278 3308 3890 3920 5249 Size: 30 165 30 1875 30 582 30 1329 Cuts at: 5249 5279 1178 Size: 30 4072 Fragments arranged by size: 4072 1875 1329 582 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 3305 5330 1186 Size: 934 1185 2025 4029 Fragments arranged by size: 4029 2025 1185 934 BseYI C'CCAG_C Cuts at: 2169 3387 3703 4711 4966 7376 7872 2169 Size: 1218 316 1008 255 2410 496 2470 Fragments arranged by size: 2470 2410 1218 1008 496 316 255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 4125 4796 5237 5324 1163 Size: 573 609 1780 671 441 87 4012 Fragments arranged by size: 4012 1780 671 609 573 441 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5623 6384 7412 1540 Size: 275 186 3622 761 1028 2301 Fragments arranged by size: 3622 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2827 3204 5052 5241 5442 6234 6319 7390 2827 Size: 377 1848 189 201 792 85 1071 3610 Fragments arranged by size: 3610 1848 1071 792 377 201 189 85 BsmI GAATG_Cn' Cuts at: 3043 3136 5684 5777 3043 Size: 93 2548 93 5439 Fragments arranged by size: 5439 2548 93 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2104 5269 2104 Size: 3165 5008 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2827 2887 2914 3204 3582 3621 5052 Size: 399 1037 60 27 290 378 39 1431 Cuts at: 5052 5177 5193 5241 5279 5338 5442 5452 5492 Size: 125 16 48 38 59 104 10 40 Cuts at: 5492 6234 6319 7390 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1431 1071 1037 742 399 378 290 125 104 85 60 59 48 40 39 38 27 16 10 BspEI T'CCGG_A Cuts at: 7836 7836 Size: 8173 BspHI T'CATG_A Cuts at: 6062 7792 6062 Size: 1730 6443 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4062 4489 4746 5061 5259 5528 844 Size: 3218 427 257 315 198 269 3489 Fragments arranged by size: 3489 3218 427 315 269 257 198 BsrBI CCG'CTC Cuts at: 1394 1823 3775 4930 6060 1394 Size: 429 1952 1155 1130 3507 Fragments arranged by size: 3507 1952 1155 1130 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 6668 6842 65 Size: 1183 2900 2520 174 1396 Fragments arranged by size: 2900 2520 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2874 3613 4817 6817 7004 1700 Size: 110 1064 739 1204 2000 187 2869 Fragments arranged by size: 2869 2000 1204 1064 739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8173 BssSI C'ACGA_G Cuts at: 2072 5384 6233 7245 2072 Size: 3312 849 1012 3000 Fragments arranged by size: 3312 3000 1012 849 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8173 BstBI TT'CG_AA Cuts at: 2007 6977 2007 Size: 4970 3203 BstEII G'GTnAC_C Cuts at: 1184 4004 4273 1184 Size: 2820 269 5084 Fragments arranged by size: 5084 2820 269 BstXI CCAn_nnnn'nTGG Cuts at: 2512 3895 4918 5418 2512 Size: 1383 1023 500 5267 Fragments arranged by size: 5267 1383 1023 500 BstYI r'GATC_y Cuts at: 1972 2121 2334 2544 3352 4136 4441 4953 5503 Size: 149 213 210 808 784 305 512 550 Cuts at: 5503 5870 6255 6272 7724 7810 7822 8168 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 808 784 550 512 385 367 346 305 213 210 149 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3573 3763 4014 513 Size: 552 2508 190 251 4672 Fragments arranged by size: 4672 2508 552 251 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3209 3265 3568 487 Size: 683 56 1983 56 303 5092 Fragments arranged by size: 5092 1983 683 303 56 56 BtsI GCAGTG_nn' Cuts at: 2051 3269 4736 5008 5078 5689 6483 6503 2051 Size: 1218 1467 272 70 611 794 20 3721 Fragments arranged by size: 3721 1467 1218 794 611 272 70 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8138 DraI TTT'AAA Cuts at: 5477 5477 Size: 8173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7180 809 Size: 507 5864 1802 Fragments arranged by size: 5864 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2297 2876 Size: 54 1425 50 275 18 456 11 579 Cuts at: 2876 3252 3680 4377 5314 5620 6509 8138 8 Size: 376 428 697 937 306 889 1629 43 Fragments arranged by size: 1629 1425 937 889 697 579 456 428 376 306 275 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 5620 1537 Size: 275 3808 4090 Fragments arranged by size: 4090 3808 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2453 2810 3162 3765 3933 4920 5348 6103 Size: 1100 357 352 603 168 987 428 755 Cuts at: 6103 1353 Size: 3423 Fragments arranged by size: 3423 1100 987 755 603 428 357 352 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6760 7134 7280 387 Size: 1064 138 5171 374 146 1280 Fragments arranged by size: 5171 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2825 3202 2825 Size: 377 7796 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2519 2877 3003 3110 4289 4632 Size: 393 469 418 358 126 107 1179 343 Cuts at: 4632 4650 4940 4970 4999 5015 5066 5171 5263 Size: 18 290 30 29 16 51 105 92 Cuts at: 5263 5474 6236 6833 7620 7888 1239 Size: 211 762 597 787 268 1524 Fragments arranged by size: 1524 1179 787 762 597 469 418 393 358 343 290 268 211 126 107 105 92 51 30 29 18 16 EcoNI CCTnn'n_nnAGG Cuts at: 3436 3436 Size: 8173 EcoO109I rG'GnC_Cy Cuts at: 2653 3300 3970 5189 5190 5334 5335 2653 Size: 647 670 1219 1 144 1 5491 Fragments arranged by size: 5491 1219 670 647 144 1 1 EcoRI G'AATT_C Cuts at: 5482 5860 5482 Size: 378 7795 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4876 4876 Size: 8173 FspI TGC'GCA Cuts at: 6679 6679 Size: 8173 HaeII r_GCGC'y Cuts at: 1678 3184 3923 5066 7320 1678 Size: 1506 739 1143 2254 2531 Fragments arranged by size: 2531 2254 1506 1143 739 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 3491 3523 3890 Size: 32 135 32 695 32 1192 32 367 Cuts at: 3890 3922 4101 4133 4160 4192 5249 5281 6814 Size: 32 179 32 27 32 1057 32 1533 Cuts at: 6814 6846 6888 6920 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1533 1192 1057 695 367 179 135 42 32 32 32 32 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 3720 5517 5763 669 Size: 988 281 1782 1797 246 3079 Fragments arranged by size: 3079 1797 1782 988 281 246 HindIII A'AGCT_T Cuts at: 748 3629 3697 748 Size: 2881 68 5224 Fragments arranged by size: 5224 2881 68 HpaI GTT'AAC Cuts at: 5763 5763 Size: 8173 KasI G'GCGC_C Cuts at: 5062 5062 Size: 8173 KpnI G_GTAC'C Cuts at: 5498 5498 Size: 8173 MfeI C'AATT_G Cuts at: 5772 5772 Size: 8173 MluI A'CGCG_T Cuts at: 8049 8049 Size: 8173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3489 7287 7471 1305 Size: 2184 3798 184 2007 Fragments arranged by size: 3798 2184 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2299 4379 10 Size: 54 1425 343 467 2080 3804 Fragments arranged by size: 3804 2080 1425 467 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2708 3762 4400 5445 6131 6490 Size: 648 1248 294 1054 638 1045 686 359 Cuts at: 6490 6649 518 Size: 159 2042 Fragments arranged by size: 2042 1248 1054 1045 686 648 638 359 294 159 MspA1I CmG'CkG Cuts at: 1606 3829 3891 4918 5059 5589 6266 7414 7659 Size: 2223 62 1027 141 530 677 1148 245 Cuts at: 7659 1606 Size: 2120 Fragments arranged by size: 2223 2120 1148 1027 677 530 245 141 62 NaeI GCC'GGC Cuts at: 4819 4819 Size: 8173 NarI GG'CG_CC Cuts at: 5063 5063 Size: 8173 NcoI C'CATG_G Cuts at: 513 1065 3573 513 Size: 552 2508 5113 Fragments arranged by size: 5113 2508 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8173 NgoMIV G'CCGG_C Cuts at: 4817 4817 Size: 8173 NheI G'CTAG_C Cuts at: 1052 3671 1052 Size: 2619 5554 NotI GC'GGCC_GC Cuts at: 5620 5620 Size: 8173 NspI r_CATG'y Cuts at: 1132 1229 2044 2638 2707 3650 4667 5531 7076 Size: 97 815 594 69 943 1017 864 1545 Cuts at: 7076 1132 Size: 2229 Fragments arranged by size: 2229 1545 1017 943 864 815 594 97 69 PasI CC'CwG_GG Cuts at: 1348 4654 4883 1348 Size: 3306 229 4638 Fragments arranged by size: 4638 3306 229 PciI A'CATG_T Cuts at: 2703 7072 2703 Size: 4369 3804 PflMI CCAn_nnn'nTGG Cuts at: 1108 2089 2295 2452 3421 4834 1108 Size: 981 206 157 969 1413 4447 Fragments arranged by size: 4447 1413 981 969 206 157 PfoI T'CCnGG_A Cuts at: 3318 4743 4872 5043 5217 3318 Size: 1425 129 171 174 6274 Fragments arranged by size: 6274 1425 174 171 129 PmeI GTTT'AAAC Cuts at: 5477 5477 Size: 8173 PmlI CAC'GTG Cuts at: 2323 2647 4573 5443 2323 Size: 324 1926 870 5053 Fragments arranged by size: 5053 1926 870 324 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2471 2503 6242 6274 7755 7787 2471 Size: 32 3739 32 1481 32 2857 Fragments arranged by size: 3739 2857 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2653 3970 2653 Size: 1317 6856 PshAI GACnn'nnGTC Cuts at: 1613 3472 3962 1613 Size: 1859 490 5824 Fragments arranged by size: 5824 1859 490 PsiI TTA'TAA Cuts at: 5743 5743 Size: 8173 PspOMI G'GGCC_C Cuts at: 5189 5334 5189 Size: 145 8028 PstI C_TGCA'G Cuts at: 830 2385 2667 2682 4116 4440 5058 5073 5525 Size: 1555 282 15 1434 324 618 15 452 Cuts at: 5525 830 Size: 3478 Fragments arranged by size: 3478 1555 1434 618 452 324 282 15 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8173 PvuII CAG'CTG Cuts at: 3829 4918 5059 3829 Size: 1089 141 6943 Fragments arranged by size: 6943 1089 141 SacI G_AGCT'C Cuts at: 2827 3204 2827 Size: 377 7796 SalI G'TCGA_C Cuts at: 1936 5515 1936 Size: 3579 4594 SanDI GG'GwC_CC Cuts at: 3970 3970 Size: 8173 SapI GCTCTTCn'nnn_ Cuts at: 3765 4920 3765 Size: 1155 7018 SbfI CC_TGCA'GG Cuts at: 2667 4440 5525 2667 Size: 1773 1085 5315 Fragments arranged by size: 5315 1773 1085 ScaI AGT'ACT Cuts at: 2947 6421 8133 2947 Size: 3474 1712 2987 Fragments arranged by size: 3474 2987 1712 SfcI C'TryA_G Cuts at: 826 1045 2191 2381 2663 2678 3184 3337 3485 Size: 219 1146 190 282 15 506 153 148 Cuts at: 3485 4112 4436 4564 5054 5069 5521 6656 7337 Size: 627 324 128 490 15 452 1135 681 Cuts at: 7337 7528 826 Size: 191 1471 Fragments arranged by size: 1471 1146 1135 681 627 506 490 452 324 282 219 191 190 153 148 128 15 15 SfoI GGC'GCC Cuts at: 5064 5064 Size: 8173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8173 SgrAI Cr'CCGG_yG Cuts at: 1700 3613 4817 1700 Size: 1913 1204 5056 Fragments arranged by size: 5056 1913 1204 SmaI CCC'GGG Cuts at: 1872 4620 5500 1872 Size: 2748 880 4545 Fragments arranged by size: 4545 2748 880 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 6277 7178 7440 820 Size: 197 926 2378 1956 901 262 1553 Fragments arranged by size: 2378 1956 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5470 5964 493 Size: 4977 494 2702 Fragments arranged by size: 4977 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8173 SphI G_CATG'C Cuts at: 1132 2638 5531 1132 Size: 1506 2893 3774 Fragments arranged by size: 3774 2893 1506 SrfI GCCC'GGGC Cuts at: 4620 4620 Size: 8173 SspI AAT'ATT Cuts at: 5 52 4243 6097 5 Size: 47 4191 1854 2081 Fragments arranged by size: 4191 2081 1854 47 StuI AGG'CCT Cuts at: 1517 4947 5465 1517 Size: 3430 518 4225 Fragments arranged by size: 4225 3430 518 StyI C'CwwG_G Cuts at: 513 1065 2289 2626 3573 5612 513 Size: 552 1224 337 947 2039 3074 Fragments arranged by size: 3074 2039 1224 947 552 337 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3282 6200 6217 6370 6555 7000 325 Size: 1255 1702 2918 17 153 185 445 1498 Fragments arranged by size: 2918 1702 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2945 6419 8131 Size: 274 80 33 51 1443 968 3474 1712 Cuts at: 8131 96 Size: 138 Fragments arranged by size: 3474 1712 1443 968 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2753 2785 3278 3310 3616 3648 4449 4481 8133 Size: 32 493 32 306 32 801 32 3652 Cuts at: 8133 8165 2753 Size: 32 2761 Fragments arranged by size: 3652 2761 801 493 306 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5261 1316 Size: 3945 4228 XbaI T'CTAG_A Cuts at: 5509 5509 Size: 8173 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3265 4202 3265 Size: 937 7236 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8173 XmaI C'CCGG_G Cuts at: 1870 4618 5498 1870 Size: 2748 880 4545 Fragments arranged by size: 4545 2748 880 XmnI GAAnn'nnTTC Cuts at: 2114 3123 4092 6302 2114 Size: 1009 969 2210 3985 Fragments arranged by size: 3985 2210 1009 969 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5981 276 Size: 53 83 186 936 4447 2468 Fragments arranged by size: 4447 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SwaI