Restriction map for
pFN21ASDA1874
(Circular) (Six-base) MAPSORT of: pFN21ASDA1874.seq Check: 8333 from: 1 to: 6292
pFN21ASDA1874 6292 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 3635 1937
Size: 664 1034 4594
Fragments arranged by size:
4594 1034 664
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2661 3346 3594 4355 5739 6007 1632
Size: 744 285 685 248 761 1384 268 1917
Fragments arranged by size:
1917 1384 761 744 685 285 268 248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2437 1184
Size: 1253 5039
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 2563 2907 2976 3312 3396 3601 3827 3979
Size: 1070 344 69 336 84 205 226 152
Cuts at: 3979 6088 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1070 344 336 226 205 152
84 69
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 1870
Size: 73 1674 4545
Fragments arranged by size:
4545 1674 73
BamHI G'GATC_C
Cuts at: 2209 3622 3989 2209
Size: 1413 367 4512
Fragments arranged by size:
4512 1413 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3613 5068 618
Size: 325 217 627 1826 1455 1842
Fragments arranged by size:
1842 1826 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 3374 3611 1391
Size: 671 1312 237 4072
Fragments arranged by size:
4072 1312 671 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2649 928
Size: 971 159 591 4571
Fragments arranged by size:
4571 971 591 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 4353 5509 1790
Size: 2563 1156 2573
Fragments arranged by size:
2573 2563 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2841 3090 3174 3258 3342 4952 1239
Size: 1016 586 249 84 84 84 1610 2579
Fragments arranged by size:
2579 1610 1016 586 249 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 4417 5282 5580 2345
Size: 2072 865 298 3057
Fragments arranged by size:
3057 2072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2986 4955 882
Size: 617 1487 1969 2219
Fragments arranged by size:
2219 1969 1487 617
BsaAI yAC'GTr
Cuts at: 493 1204 3589 4083 493
Size: 711 2385 494 2702
Fragments arranged by size:
2702 2385 711 494
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4099 4481 5116 275
Size: 53 83 186 936 2566 382 635 1451
Fragments arranged by size:
2566 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3488 4725 5123 5397 5544 5955 3488
Size: 1237 398 274 147 411 3825
Fragments arranged by size:
3825 1237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 1186
Size: 1021 5271
BseYI C'CCAG_C
Cuts at: 5495 5991 5495
Size: 496 5796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2199 3068 3556 1163
Size: 573 463 869 488 3899
Fragments arranged by size:
3899 869 573 488 463
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2062 2102 3290 3374 4353 4438 5509 2062
Size: 40 1188 84 979 85 1071 2845
Fragments arranged by size:
2845 1188 1071 979 85 84 40
BsmI GAATG_Cn'
Cuts at: 2924 2933 3008 3260 3803 3896 2924
Size: 9 75 252 543 93 5320
Fragments arranged by size:
5320 543 252 93 75 9
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2102 3290 3374 3611 4353 4438
Size: 399 272 40 1188 84 237 742 85
Cuts at: 4438 5509 1391
Size: 1071 2174
Fragments arranged by size:
2174 1188 1071 742 399 272 237 85
84 40
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 4181 5911 4181
Size: 1730 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 3647 844
Size: 2002 801 3489
Fragments arranged by size:
3489 2002 801
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2848 3013 3199 4787 4961 65
Size: 1183 1600 165 186 1588 174 1396
Fragments arranged by size:
1600 1588 1396 1183 186 174 165
BsrFI r'CCGG_y
Cuts at: 1700 1810 4936 5123 1700
Size: 110 3126 187 2869
Fragments arranged by size:
3126 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2928 794
Size: 2134 4158
BstBI TT'CG_AA
Cuts at: 2007 5096 2007
Size: 3089 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstXI CCAn_nnnn'nTGG
Cuts at: 2196 2196
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 2141 2209 2660 3622 3989 4374 4391 5843
Size: 169 68 451 962 367 385 17 1452
Cuts at: 5843 5929 5941 6287 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 962 451 385 367 346 169
86 68 17 12
BstZ17I GTA'TAC
Cuts at: 2602 2602
Size: 6292
BtgI C'CryG_G
Cuts at: 513 1065 2022 2175 513
Size: 552 957 153 4630
Fragments arranged by size:
4630 957 552 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3265 3808 4602 4622 2929
Size: 336 543 794 20 4599
Fragments arranged by size:
4599 794 543 336 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2830 2865 2995 3030 529
Size: 35 2266 35 130 35 3791
Fragments arranged by size:
3791 2266 130 35 35 35
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2172 3739
Size: 54 1425 50 275 18 306 36 1567
Cuts at: 3739 4628 6257 8
Size: 889 1629 43
Fragments arranged by size:
1629 1567 1425 889 306 275 54 50
43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2100 2780 3570 4222 1353
Size: 737 10 680 790 652 3423
Fragments arranged by size:
3423 790 737 680 652 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2060 3372 2060
Size: 1312 4980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2376 2661 2841 3090 3174 3258
Size: 393 623 121 285 180 249 84 84
Cuts at: 3258 3342 3346 3594 4355 4952 5739 6007 1239
Size: 84 4 248 761 597 787 268 1524
Fragments arranged by size:
1524 787 761 623 597 393 285 268
249 248 180 121 84 84 84 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2052 2052
Size: 6292
EcoRI G'AATT_C
Cuts at: 2563 2907 2976 3312 3396 3601 3979 2563
Size: 344 69 336 84 205 378 4876
Fragments arranged by size:
4876 378 344 336 205 84 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2504 2956 2504
Size: 452 5840
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 5439 1678
Size: 3761 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3277 3309 4933 4965 5007
Size: 32 135 32 1705 32 1624 32 42
Cuts at: 5007 5039 1373
Size: 32 2626
Fragments arranged by size:
2626 1705 1624 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2321 2611 2689 3105 3526 748
Size: 1573 290 78 416 421 3514
Fragments arranged by size:
3514 1573 421 416 290 78
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2965 5406 5590 1305
Size: 1660 2441 184 2007
Fragments arranged by size:
2441 2007 1660 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 10
Size: 54 1425 343 342 4128
Fragments arranged by size:
4128 1425 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 2592 3253 4250 4609 4768 518
Size: 648 1392 34 661 997 359 159 2042
Fragments arranged by size:
2042 1392 997 661 648 359 159 34
MspA1I CmG'CkG
Cuts at: 1606 3708 4385 5533 5778 1606
Size: 2102 677 1148 245 2120
Fragments arranged by size:
2120 2102 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2175 513
Size: 552 957 153 4630
Fragments arranged by size:
4630 957 552 153
NdeI CA'TA_TG
Cuts at: 387 3250 387
Size: 2863 3429
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2296 1108
Size: 1188 5104
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2272 4361 4393 5874 5906 2240
Size: 32 2089 32 1481 32 2626
Fragments arranged by size:
2626 2089 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 2733 3168 3862 2733
Size: 435 694 5163
Fragments arranged by size:
5163 694 435
PstI C_TGCA'G
Cuts at: 830 3644 830
Size: 2814 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
SacI G_AGCT'C
Cuts at: 2062 3374 2062
Size: 1312 4980
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 3570 2090
Size: 1480 4812
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2242 3640 4775 5456 5647 826
Size: 219 1197 1398 1135 681 191 1471
Fragments arranged by size:
1471 1398 1197 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 4396 5297 5559 820
Size: 197 926 381 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 381 262 197
SnaBI TAC'GTA
Cuts at: 493 3589 4083 493
Size: 3096 494 2702
Fragments arranged by size:
3096 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2022 2175 3731 513
Size: 552 957 153 1556 3074
Fragments arranged by size:
3074 1556 957 552 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4319 4336 4489 4674 5119 325
Size: 1255 2739 17 153 185 445 1498
Fragments arranged by size:
2739 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3035 4538 6250
Size: 274 80 33 51 1443 1058 1503 1712
Cuts at: 6250 96
Size: 138
Fragments arranged by size:
1712 1503 1443 1058 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 2612 2939 3359 4421 2612
Size: 327 420 1062 4483
Fragments arranged by size:
4483 1062 420 327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI