Restriction map for
pFN21ASDA1869
(Circular) (Six-base) MAPSORT of: pFN21ASDA1869.seq Check: 6063 from: 1 to: 7519
pFN21ASDA1869 7519 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4491 4491
Size: 7519
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 4728 5329 278
Size: 53 83 186 936 2392 800 601 2468
Fragments arranged by size:
2468 2392 936 800 601 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2730 3558 4862 1937
Size: 793 828 1304 4594
Fragments arranged by size:
4594 1304 828 793
Acc65I G'GTAC_C
Cuts at: 4840 4840
Size: 7519
AclI AA'CG_TT
Cuts at: 1563 5646 6019 1563
Size: 4083 373 3063
Fragments arranged by size:
4083 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3395 4769 5582 6966 7234 1632
Size: 1253 510 1374 813 1384 268 1917
Fragments arranged by size:
1917 1384 1374 1253 813 510 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7322
AflIII A'CryG_T
Cuts at: 1203 6418 7395 1203
Size: 5215 977 1327
Fragments arranged by size:
5215 1327 977
AgeI A'CCGG_T
Cuts at: 6350 6350
Size: 7519
AhdI GACnn_n'nnGTC
Cuts at: 6248 6248
Size: 7519
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7519
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 7487
AlwNI CAG_nnn'CTG
Cuts at: 3654 3884 5262 6834 3654
Size: 230 1378 1572 4339
Fragments arranged by size:
4339 1572 1378 230
ApaI G_GGCC'C
Cuts at: 2643 3286 4007 4254 4600 4770 2643
Size: 643 721 247 346 170 5392
Fragments arranged by size:
5392 721 643 346 247 170
ApaLI G'TGCA_C
Cuts at: 5576 6732 5576
Size: 1156 6363
ApoI r'AATT_y
Cuts at: 1493 4828 5054 5206 7315 1493
Size: 3335 226 152 2109 1697
Fragments arranged by size:
3335 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6073 160
Size: 872 5041 1606
Fragments arranged by size:
5041 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7519
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 2799 2832 3177 4124 4295 4493
Size: 73 310 546 33 345 947 171 198
Cuts at: 4493 4617 4627 4658 4688 4844 1870
Size: 124 10 31 30 156 4545
Fragments arranged by size:
4545 947 546 345 310 198 171 156
124 73 33 31 30 10
BamHI G'GATC_C
Cuts at: 3134 4849 5216 3134
Size: 1715 367 5437
Fragments arranged by size:
5437 1715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 3872 4840 6295 618
Size: 325 217 627 521 1564 968 1455 1842
Fragments arranged by size:
1842 1564 1455 968 627 521 325 217
BanII G_rGCy'C
Cuts at: 1391 2643 3286 3417 3957 4007 4014 4254 4380
Size: 1252 643 131 540 50 7 240 126
Cuts at: 4380 4419 4600 4770 4838 1391
Size: 39 181 170 68 4072
Fragments arranged by size:
4072 1252 643 540 240 181 170 131
126 68 50 39 7
BbeI G_GCGC'C
Cuts at: 3876 3876
Size: 7519
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2968 928
Size: 971 1069 5479
Fragments arranged by size:
5479 1069 971
BbvCI CC'TCA_GC
Cuts at: 4475 4475
Size: 7519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 5710 5744 1903
Size: 34 1985 34 1754 34 3678
Fragments arranged by size:
3678 1985 1754 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5411 6621 5411
Size: 1210 6309
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3851 4477 6130
Size: 107 122 71 631 605 2179 626 1653
Cuts at: 6130 136
Size: 1525
Fragments arranged by size:
2179 1653 1525 631 626 605 122 107
71
BglII A'GATC_T
Cuts at: 7514 7514
Size: 7519
BlpI GC'TnA_GC
Cuts at: 4936 4936
Size: 7519
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2313 2643 3286 3583 4007 4095 4239
Size: 245 278 330 643 297 424 88 144
Cuts at: 4239 4254 4491 4600 4770 5580 6736 1790
Size: 15 237 109 170 810 1156 2573
Fragments arranged by size:
2573 1156 810 643 424 330 297 278
245 237 170 144 109 88 15
BmgBI CAC'GTC
Cuts at: 1318 4486 1318
Size: 3168 4351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 2943 3756
Size: 416 53 83 399 24 1122 404 813
Cuts at: 3756 4732 5250 6208 442
Size: 976 518 958 1753
Fragments arranged by size:
1753 1122 976 958 813 518 416 404
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4483 4483
Size: 7519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 3524 3631 4343 4624 6179 1239
Size: 953 1332 107 712 281 1555 2579
Fragments arranged by size:
2579 1555 1332 953 712 281 107
Bpu10I CC'TnA_GC
Cuts at: 3411 4475 3411
Size: 1064 6455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5644 6509 6807 5644
Size: 865 298 6356
Fragments arranged by size:
6356 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2715 2755 3174 3901 6182 882
Size: 617 1216 40 419 727 2281 2219
Fragments arranged by size:
2281 2219 1216 727 617 419 40
BsaAI yAC'GTr
Cuts at: 493 1204 4816 5310 493
Size: 711 3612 494 2702
Fragments arranged by size:
3612 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3690 7513 3690
Size: 3823 3696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3873 3925 4049 4557
Size: 53 83 186 936 2340 52 124 508
Cuts at: 4557 4725 5326 5708 6343 275
Size: 168 601 382 635 1451
Fragments arranged by size:
2340 1451 936 635 601 508 382 186
168 124 83 53 52
BsaWI w'CCGG_w
Cuts at: 2232 4081 5952 6350 6624 6771 7182 2232
Size: 1849 1871 398 274 147 411 2569
Fragments arranged by size:
2569 1871 1849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 2712 2742 1178
Size: 30 165 30 849 30 430 30 5955
Fragments arranged by size:
5955 849 430 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3088 3160 3616 3827 4762 1186
Size: 1902 72 456 211 935 3943
Fragments arranged by size:
3943 1902 935 456 211 72
BseYI C'CCAG_C
Cuts at: 2643 2770 3698 3802 4442 4552 6722 7218 2643
Size: 127 928 104 640 110 2170 496 2944
Fragments arranged by size:
2944 2170 928 640 496 127 110 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 3162 3478 4064 4328 1163
Size: 573 1065 361 316 586 264 4354
Fragments arranged by size:
4354 1065 586 573 361 316 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4801 4969 5730 6758 1540
Size: 275 186 2800 168 761 1028 2301
Fragments arranged by size:
2800 2301 1028 761 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 5580 5665 6736 5580
Size: 85 1071 6363
Fragments arranged by size:
6363 1071 85
BsmI GAATG_Cn'
Cuts at: 5030 5123 5030
Size: 93 7426
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2313 2643 3286 3417 3583 3957
Size: 399 245 278 330 643 131 166 374
Cuts at: 3957 4007 4014 4095 4239 4254 4380 4419 4491
Size: 50 7 81 144 15 126 39 72
Cuts at: 4491 4600 4770 4838 5580 5665 6736 1391
Size: 109 170 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 374 330 278
245 170 166 144 131 126 109 85
81 72 68 50 39 15 7
BspEI T'CCGG_A
Cuts at: 4081 7182 4081
Size: 3101 4418
BspHI T'CATG_A
Cuts at: 5408 7138 5408
Size: 1730 5789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2955 3804 4350 4491 4874 844
Size: 2111 849 546 141 383 3489
Fragments arranged by size:
3489 2111 849 546 383 141
BsrBI CCG'CTC
Cuts at: 1394 1823 2658 2958 3819 4224 4544 5406 1394
Size: 429 835 300 861 405 320 862 3507
Fragments arranged by size:
3507 862 861 835 429 405 320 300
BsrDI GCAATG_nn'
Cuts at: 65 1248 6014 6188 65
Size: 1183 4766 174 1396
Fragments arranged by size:
4766 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3573 4144 4683 6163 6350 1700
Size: 110 1763 571 539 1480 187 2869
Fragments arranged by size:
2869 1763 1480 571 539 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7519
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7519
BssSI C'ACGA_G
Cuts at: 4457 5579 6591 4457
Size: 1122 1012 5385
Fragments arranged by size:
5385 1122 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3863 794
Size: 3069 4450
BstBI TT'CG_AA
Cuts at: 2007 6323 2007
Size: 4316 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7519
BstXI CCAn_nnnn'nTGG
Cuts at: 2157 3424 2157
Size: 1267 6252
BstYI r'GATC_y
Cuts at: 1972 3134 3671 4849 5216 5601 5618 7070 7156
Size: 1162 537 1178 367 385 17 1452 86
Cuts at: 7156 7168 7514 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1178 1162 537 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3714 3994 4305 513
Size: 552 2649 280 311 3727
Fragments arranged by size:
3727 2649 552 311 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6780
Fragments arranged by size:
6780 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 5035 5829 5849 3466
Size: 1569 794 20 5136
Fragments arranged by size:
5136 1569 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7484
DraI TTT'AAA
Cuts at: 4823 4823
Size: 7519
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3100 6526 809
Size: 507 1784 3426 1802
Fragments arranged by size:
3426 1802 1784 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2556 2953 3843
Size: 54 1425 50 275 18 726 397 890
Cuts at: 3843 3852 4588 4798 4966 5855 7484 8
Size: 9 736 210 168 889 1629 43
Fragments arranged by size:
1629 1425 890 889 736 726 397 275
210 168 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4798 4966 1537
Size: 275 2986 168 4090
Fragments arranged by size:
4090 2986 275 168
EarI CTCTTCn'nnn_
Cuts at: 1353 3178 3423 5449 1353
Size: 1825 245 2026 3423
Fragments arranged by size:
3423 2026 1825 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2650 4525 4578 6106 6480 6626
Size: 1064 138 1061 1875 53 1528 374 146
Cuts at: 6626 387
Size: 1280
Fragments arranged by size:
1875 1528 1280 1064 1061 374 146 138
53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2885 3395 3524 3631 4343 4624
Size: 393 560 693 510 129 107 712 281
Cuts at: 4624 4769 5582 6179 6966 7234 1239
Size: 145 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 712 693 597 560 510
393 281 268 145 129 107
EcoNI CCTnn'n_nnAGG
Cuts at: 3124 3598 3124
Size: 474 7045
EcoO109I rG'GnC_Cy
Cuts at: 2107 2167 2640 3087 3282 3283 3407 3591 3622
Size: 60 473 447 195 1 124 184 31
Cuts at: 3622 3793 4003 4171 4452 4597 4634 4655 4767
Size: 171 210 168 281 145 37 21 112
Cuts at: 4767 2107
Size: 4859
Fragments arranged by size:
4859 473 447 281 210 195 184 171
168 145 124 112 60 37 31 21
1
EcoRI G'AATT_C
Cuts at: 4828 5206 4828
Size: 378 7141
EcoRV GAT'ATC
Cuts at: 3664 3664
Size: 7519
FseI GG_CCGG'CC
Cuts at: 4687 4687
Size: 7519
FspI TGC'GCA
Cuts at: 6025 6025
Size: 7519
HaeII r_GCGC'y
Cuts at: 1678 3876 4406 6666 1678
Size: 2198 530 2260 2531
Fragments arranged by size:
2531 2260 2198 530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2712 2744 6160 6192 6234
Size: 32 135 32 1140 32 3416 32 42
Cuts at: 6234 6266 1373
Size: 32 2626
Fragments arranged by size:
3416 2626 1140 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4863 5109 669
Size: 988 281 2925 246 3079
Fragments arranged by size:
3079 2925 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2867 748
Size: 2119 5400
HpaI GTT'AAC
Cuts at: 5109 5109
Size: 7519
KasI G'GCGC_C
Cuts at: 3872 3872
Size: 7519
KpnI G_GTAC'C
Cuts at: 4844 4844
Size: 7519
MfeI C'AATT_G
Cuts at: 5118 5118
Size: 7519
MluI A'CGCG_T
Cuts at: 7395 7395
Size: 7519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 6633 6817 1305
Size: 1269 4059 184 2007
Fragments arranged by size:
4059 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 3845 10
Size: 54 1425 343 726 1287 3684
Fragments arranged by size:
3684 1425 1287 726 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3341 5477 5836 5995 518
Size: 648 2175 2136 359 159 2042
Fragments arranged by size:
2175 2136 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2594 3223 3236 4474 4935 5612 6760 7005
Size: 988 629 13 1238 461 677 1148 245
Cuts at: 7005 1606
Size: 2120
Fragments arranged by size:
2120 1238 1148 988 677 629 461 245
13
NaeI GCC'GGC
Cuts at: 4685 4685
Size: 7519
NarI GG'CG_CC
Cuts at: 3873 3873
Size: 7519
NcoI C'CATG_G
Cuts at: 513 1065 3714 513
Size: 552 2649 4318
Fragments arranged by size:
4318 2649 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7519
NgoMIV G'CCGG_C
Cuts at: 4683 4683
Size: 7519
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7519
NotI GC'GGCC_GC
Cuts at: 4966 4966
Size: 7519
NspI r_CATG'y
Cuts at: 1132 1229 4877 6422 1132
Size: 97 3648 1545 2229
Fragments arranged by size:
3648 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2412 2891 3764 3868 4176 1348
Size: 1064 479 873 104 308 4691
Fragments arranged by size:
4691 1064 873 479 308 104
PciI A'CATG_T
Cuts at: 6418 6418
Size: 7519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 2710 3632 1108
Size: 1236 366 922 4995
Fragments arranged by size:
4995 1236 922 366
PfoI T'CCnGG_A
Cuts at: 3423 3423
Size: 7519
PmeI GTTT'AAAC
Cuts at: 4823 4823
Size: 7519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 5588 5620 7101 7133 3044
Size: 32 2512 32 1481 32 3430
Fragments arranged by size:
3430 2512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 3407 3622 3793 4171 4634 4655 2107
Size: 1300 215 171 378 463 21 4971
Fragments arranged by size:
4971 1300 463 378 215 171 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7519
PsiI TTA'TAA
Cuts at: 5089 5089
Size: 7519
PspOMI G'GGCC_C
Cuts at: 2639 3282 4003 4250 4596 4766 2639
Size: 643 721 247 346 170 5392
Fragments arranged by size:
5392 721 643 346 247 170
PspXI vC'TCGA_Gb
Cuts at: 4617 4617
Size: 7519
PstI C_TGCA'G
Cuts at: 830 2194 2493 4871 830
Size: 1364 299 2378 3478
Fragments arranged by size:
3478 2378 1364 299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7519
PvuII CAG'CTG
Cuts at: 2594 2594
Size: 7519
SalI G'TCGA_C
Cuts at: 1936 4861 1936
Size: 2925 4594
SanDI GG'GwC_CC
Cuts at: 4171 4655 4171
Size: 484 7035
SbfI CC_TGCA'GG
Cuts at: 4871 4871
Size: 7519
ScaI AGT'ACT
Cuts at: 5767 7479 5767
Size: 1712 5807
SfcI C'TryA_G
Cuts at: 826 1045 2190 2489 2619 3442 4867 6002 6683
Size: 219 1145 299 130 823 1425 1135 681
Cuts at: 6683 6874 826
Size: 191 1471
Fragments arranged by size:
1471 1425 1145 1135 823 681 299 219
191 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 3851 3851
Size: 7519
SfoI GGC'GCC
Cuts at: 3874 3874
Size: 7519
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7519
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7519
SmaI CCC'GGG
Cuts at: 1872 2255 4297 4660 4846 1872
Size: 383 2042 363 186 4545
Fragments arranged by size:
4545 2042 383 363 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4617 5623 6524 6786 820
Size: 197 926 2674 1006 901 262 1553
Fragments arranged by size:
2674 1553 1006 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4816 5310 493
Size: 4323 494 2702
Fragments arranged by size:
4323 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7519
SphI G_CATG'C
Cuts at: 1132 4877 1132
Size: 3745 3774
SspI AAT'ATT
Cuts at: 5 52 5443 5
Size: 47 5391 2081
Fragments arranged by size:
5391 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 2927 1517
Size: 1191 219 6109
Fragments arranged by size:
6109 1191 219
StyI C'CwwG_G
Cuts at: 513 1065 2635 2709 2838 3349 3714 4026 4206
Size: 552 1570 74 129 511 365 312 180
Cuts at: 4206 4958 513
Size: 752 3074
Fragments arranged by size:
3074 1570 752 552 511 365 312 180
129 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 2999 3139 3266 5546 5563 5716
Size: 1255 978 441 140 127 2280 17 153
Cuts at: 5716 5901 6346 325
Size: 185 445 1498
Fragments arranged by size:
2280 1498 1255 978 445 441 185 153
140 127 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 5765 7477
Size: 274 80 33 51 1443 603 3185 1712
Cuts at: 7477 96
Size: 138
Fragments arranged by size:
3185 1712 1443 603 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2685 2717 2840 2872 7479 7511 2252
Size: 32 401 32 123 32 4607 32 2260
Fragments arranged by size:
4607 2260 401 123 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2726 1316
Size: 1410 6109
XbaI T'CTAG_A
Cuts at: 4855 4855
Size: 7519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3633 3655 3633
Size: 22 7497
XhoI C'TCGA_G
Cuts at: 1943 4617 1943
Size: 2674 4845
XmaI C'CCGG_G
Cuts at: 1870 2253 4295 4658 4844 1870
Size: 383 2042 363 186 4545
Fragments arranged by size:
4545 2042 383 363 186
XmnI GAAnn'nnTTC
Cuts at: 2450 5648 2450
Size: 3198 4321
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 4726 5327 276
Size: 53 83 186 936 2392 800 601 2468
Fragments arranged by size:
2468 2392 936 800 601 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI FalI FspAI NruI NsiI PacI
PmlI PsrI RsrII SacI SacII SapI SexAI SrfI
SwaI