Restriction map for
pFN21ASDA1862
(Circular) (Six-base) MAPSORT of: pFN21ASDA1862.seq Check: 1002 from: 1 to: 7819
pFN21ASDA1862 7819 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5629 278
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5162 1937
Size: 3225 4594
Acc65I G'GTAC_C
Cuts at: 5140 5140
Size: 7819
AclI AA'CG_TT
Cuts at: 1563 5946 6319 1563
Size: 4383 373 3063
Fragments arranged by size:
4383 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2687 2870 3215 3949 3996 5882 7266 7534
Size: 1055 183 345 734 47 1886 1384 268
Cuts at: 7534 1632
Size: 1917
Fragments arranged by size:
1917 1886 1384 1055 734 345 268 183
47
AfeI AGC'GCT
Cuts at: 4482 4482
Size: 7819
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7622
AflIII A'CryG_T
Cuts at: 1203 6718 7695 1203
Size: 5515 977 1327
Fragments arranged by size:
5515 1327 977
AgeI A'CCGG_T
Cuts at: 6650 6650
Size: 7819
AhdI GACnn_n'nnGTC
Cuts at: 3277 6548 3277
Size: 3271 4548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2169 1184
Size: 985 6834
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2926 2958 2926
Size: 32 7787
AlwNI CAG_nnn'CTG
Cuts at: 2105 2836 3053 3098 3529 3630 4001 4149 4429
Size: 731 217 45 431 101 371 148 280
Cuts at: 4429 4822 4825 5562 7134 2105
Size: 393 3 737 1572 2790
Fragments arranged by size:
2790 1572 737 731 431 393 371 280
217 148 101 45 3
ApaI G_GGCC'C
Cuts at: 2691 3137 4307 5059 2691
Size: 446 1170 752 5451
Fragments arranged by size:
5451 1170 752 446
ApaLI G'TGCA_C
Cuts at: 5876 7032 5876
Size: 1156 6663
ApoI r'AATT_y
Cuts at: 1493 2622 5128 5354 5506 7615 1493
Size: 1129 2506 226 152 2109 1697
Fragments arranged by size:
2506 2109 1697 1129 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6373 160
Size: 872 5341 1606
Fragments arranged by size:
5341 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
AvaI C'yCGr_G
Cuts at: 1870 1943 2279 2327 2699 2861 3399 3683 3958
Size: 73 336 48 372 162 538 284 275
Cuts at: 3958 3988 5095 5144 1870
Size: 30 1107 49 4545
Fragments arranged by size:
4545 1107 538 372 336 284 275 162
73 49 48 30
BamHI G'GATC_C
Cuts at: 4180 5004 5149 5516 4180
Size: 824 145 367 6483
Fragments arranged by size:
6483 824 367 145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2694 4542 4993 5140 6595
Size: 325 217 627 907 1848 451 147 1455
Cuts at: 6595 618
Size: 1842
Fragments arranged by size:
1848 1842 1455 907 627 451 325 217
147
BanII G_rGCy'C
Cuts at: 1391 2327 2352 2536 2550 2691 2783 2859 3044
Size: 936 25 184 14 141 92 76 185
Cuts at: 3044 3137 3624 4008 4143 4307 4615 4800 4901
Size: 93 487 384 135 164 308 185 101
Cuts at: 4901 5059 5138 1391
Size: 158 79 4072
Fragments arranged by size:
4072 936 487 384 308 185 185 184
164 158 141 135 101 93 92 79
76 25 14
BbeI G_GCGC'C
Cuts at: 4546 4546
Size: 7819
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2804 3990 4128 4264 928
Size: 971 905 1186 138 136 4483
Fragments arranged by size:
4483 1186 971 905 138 136
BbvCI CC'TCA_GC
Cuts at: 3091 3589 3660 3091
Size: 498 71 7250
Fragments arranged by size:
7250 498 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2318 2352 4141 4175 5017 5051 6010
Size: 34 381 34 1789 34 842 34 959
Cuts at: 6010 6044 1903
Size: 34 3678
Fragments arranged by size:
3678 1789 959 842 381 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2951 5711 6921 2951
Size: 2760 1210 3849
Fragments arranged by size:
3849 2760 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3073 3577 3898
Size: 107 122 71 631 605 1401 504 321
Cuts at: 3898 4551 6430 136
Size: 653 1879 1525
Fragments arranged by size:
1879 1525 1401 653 631 605 504 321
122 107 71
BglII A'GATC_T
Cuts at: 7814 7814
Size: 7819
BlpI GC'TnA_GC
Cuts at: 2064 2372 5236 2064
Size: 308 2864 4647
Fragments arranged by size:
4647 2864 308
Bme1580I G_kGCm'C
Cuts at: 1790 2691 2744 3137 4307 4756 5059 5880 7036
Size: 901 53 393 1170 449 303 821 1156
Cuts at: 7036 1790
Size: 2573
Fragments arranged by size:
2573 1170 1156 901 821 449 393 303
53
BmgBI CAC'GTC
Cuts at: 1318 2745 4757 4975 1318
Size: 1427 2012 218 4162
Fragments arranged by size:
4162 2012 1427 218
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2877 3451 4314
Size: 416 53 83 399 24 1460 574 863
Cuts at: 4314 5550 6508 442
Size: 1236 958 1753
Fragments arranged by size:
1753 1460 1236 958 863 574 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7819
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2387 2387
Size: 7819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 2967 3120 3551 3733 3887 3937 4223
Size: 1574 154 153 431 182 154 50 286
Cuts at: 4223 4262 4666 4717 4752 5045 6479 1239
Size: 39 404 51 35 293 1434 2579
Fragments arranged by size:
2579 1574 1434 431 404 293 286 182
154 154 153 51 50 39 35
Bpu10I CC'TnA_GC
Cuts at: 3091 3589 3660 4002 4176 4311 4497 3091
Size: 498 71 342 174 135 186 6413
Fragments arranged by size:
6413 498 342 186 174 135 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3129 3145 3527 5944 6809 7107 3129
Size: 16 382 2417 865 298 3841
Fragments arranged by size:
3841 2417 865 382 298 16
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 3285 6482 882
Size: 617 554 1232 3197 2219
Fragments arranged by size:
3197 2219 1232 617 554
BsaAI yAC'GTr
Cuts at: 493 1204 5116 5610 493
Size: 711 3912 494 2702
Fragments arranged by size:
3912 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7813 7813
Size: 7819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4543 5626 6008 6643
Size: 53 83 186 936 3010 1083 382 635
Cuts at: 6643 275
Size: 1451
Fragments arranged by size:
3010 1451 1083 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4336 4351 6252 6650 6924 7071 7482 4336
Size: 15 1901 398 274 147 411 4673
Fragments arranged by size:
4673 1901 411 398 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2959 2989 4854 4884 1178
Size: 30 165 30 1556 30 1865 30 4113
Fragments arranged by size:
4113 1865 1556 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2964 3412 3577 3625 4372 4862 4938 5058
Size: 1778 448 165 48 747 490 76 120
Cuts at: 5058 1186
Size: 3947
Fragments arranged by size:
3947 1778 747 490 448 165 120 76
48
BseYI C'CCAG_C
Cuts at: 2549 2570 3030 3125 3129 3228 3261 3380 3929
Size: 21 460 95 4 99 33 119 549
Cuts at: 3929 3935 3941 4043 4534 4608 4815 4838 5038
Size: 6 6 102 491 74 207 23 200
Cuts at: 5038 7022 7518 2549
Size: 1984 496 2850
Fragments arranged by size:
2850 1984 549 496 491 460 207 200
119 102 99 95 74 33 23 21
6 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2237 3842 4185 4415 4845 4983
Size: 573 447 54 1605 343 230 430 138
Cuts at: 4983 5069 1163
Size: 86 3913
Fragments arranged by size:
3913 1605 573 447 430 343 230 138
86 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5269 6030 7058 1540
Size: 275 186 3268 761 1028 2301
Fragments arranged by size:
3268 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2859 4008 5088 5880 5965 7036 2859
Size: 1149 1080 792 85 1071 3642
Fragments arranged by size:
3642 1149 1080 1071 792 85
BsmI GAATG_Cn'
Cuts at: 3201 5330 5423 3201
Size: 2129 93 5597
Fragments arranged by size:
5597 2129 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2033 3686 2033
Size: 1653 6166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2327 2352 2536 2550 2691 2744 2783
Size: 399 537 25 184 14 141 53 39
Cuts at: 2783 2859 3044 3137 3624 4008 4143 4307 4615
Size: 76 185 93 487 384 135 164 308
Cuts at: 4615 4756 4800 4901 5059 5088 5138 5880 5965
Size: 141 44 101 158 29 50 742 85
Cuts at: 5965 7036 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 537 487 399 384 308
185 184 164 158 141 141 135 101
93 85 76 53 50 44 39 29
25 14
BspEI T'CCGG_A
Cuts at: 4336 4351 7482 4336
Size: 15 3131 4673
Fragments arranged by size:
4673 3131 15
BspHI T'CATG_A
Cuts at: 5708 7438 5708
Size: 1730 6089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3061 4157 4728 5174 844
Size: 2217 1096 571 446 3489
Fragments arranged by size:
3489 2217 1096 571 446
BsrBI CCG'CTC
Cuts at: 1394 1823 4018 4745 5014 5706 1394
Size: 429 2195 727 269 692 3507
Fragments arranged by size:
3507 2195 727 692 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 6314 6488 65
Size: 1183 5066 174 1396
Fragments arranged by size:
5066 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6463 6650 1700
Size: 110 4653 187 2869
Fragments arranged by size:
4653 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7819
BssSI C'ACGA_G
Cuts at: 2434 4663 5879 6891 2434
Size: 2229 1216 1012 3362
Fragments arranged by size:
3362 2229 1216 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2197 4429 4961 794
Size: 1403 2232 532 3652
Fragments arranged by size:
3652 2232 1403 532
BstBI TT'CG_AA
Cuts at: 2007 6623 2007
Size: 4616 3203
BstEII G'GTnAC_C
Cuts at: 1184 4715 1184
Size: 3531 4288
BstXI CCAn_nnnn'nTGG
Cuts at: 2931 3813 4240 4483 4556 2931
Size: 882 427 243 73 6194
Fragments arranged by size:
6194 882 427 243 73
BstYI r'GATC_y
Cuts at: 1972 2167 2232 3885 4180 4680 5004 5149 5516
Size: 195 65 1653 295 500 324 145 367
Cuts at: 5516 5901 5918 7370 7456 7468 7814 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1653 1452 500 385 367 346 324
295 195 145 86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2890 3138 3878 4984 2890
Size: 248 740 1106 5725
Fragments arranged by size:
5725 1106 740 248
BtgI C'CryG_G
Cuts at: 513 1065 2504 4595 513
Size: 552 1439 2091 3737
Fragments arranged by size:
3737 2091 1439 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7080
Fragments arranged by size:
7080 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3518 3859 4108 4801 5335 6129 6149 2799
Size: 719 341 249 693 534 794 20 4469
Fragments arranged by size:
4469 794 719 693 534 341 249 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7784
DraI TTT'AAA
Cuts at: 5123 5123
Size: 7819
DraIII CAC_nnn'GTG
Cuts at: 3317 3317
Size: 7819
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6826 809
Size: 507 5510 1802
Fragments arranged by size:
5510 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 4506 4690
Size: 54 1425 50 275 18 582 2094 184
Cuts at: 4690 5266 6155 7784 8
Size: 576 889 1629 43
Fragments arranged by size:
2094 1629 1425 889 582 576 275 184
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5266 1537
Size: 275 3454 4090
Fragments arranged by size:
4090 3454 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2104 2576 4816 5749 1353
Size: 751 472 2240 933 3423
Fragments arranged by size:
3423 2240 933 751 472
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6406 6780 6926 387
Size: 1064 138 4817 374 146 1280
Fragments arranged by size:
4817 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2857 4006 2857
Size: 1149 6670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2687 2813 2870 2967 3120 3215 3551
Size: 393 1055 126 57 97 153 95 336
Cuts at: 3551 3733 3887 3937 3949 3996 4223 4262 4666
Size: 182 154 50 12 47 227 39 404
Cuts at: 4666 4717 4752 5045 5882 6479 7266 7534 1239
Size: 51 35 293 837 597 787 268 1524
Fragments arranged by size:
1524 1055 837 787 597 404 393 336
293 268 227 182 154 153 126 97
95 57 51 50 47 39 35 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2361 2935 3492 4421 4496 4645 2361
Size: 574 557 929 75 149 5535
Fragments arranged by size:
5535 929 574 557 149 75
EcoO109I rG'GnC_Cy
Cuts at: 2607 2688 2771 2951 3050 3115 3133 3134 3387
Size: 81 83 180 99 65 18 1 253
Cuts at: 3387 3445 3900 3926 4086 4303 4518 4553 4601
Size: 58 455 26 160 217 215 35 48
Cuts at: 4601 4658 5055 5056 5092 2607
Size: 57 397 1 36 5334
Fragments arranged by size:
5334 455 397 253 217 215 180 160
99 83 81 65 58 57 48 36
35 26 18 1 1
EcoRI G'AATT_C
Cuts at: 5128 5506 5128
Size: 378 7441
FspI TGC'GCA
Cuts at: 6325 6325
Size: 7819
HaeII r_GCGC'y
Cuts at: 1678 2268 3707 3876 4484 4546 6966 1678
Size: 590 1439 169 608 62 2420 2531
Fragments arranged by size:
2531 2420 1439 608 590 169 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2959 2991 3263 3295 3706
Size: 32 135 32 1387 32 272 32 411
Cuts at: 3706 3738 6460 6492 6534 6566 1373
Size: 32 2722 32 42 32 2626
Fragments arranged by size:
2722 2626 1387 411 272 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5163 5409 669
Size: 988 281 3225 246 3079
Fragments arranged by size:
3225 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2713 4572 748
Size: 1965 1859 3995
Fragments arranged by size:
3995 1965 1859
HpaI GTT'AAC
Cuts at: 5409 5409
Size: 7819
KasI G'GCGC_C
Cuts at: 4542 4542
Size: 7819
KpnI G_GTAC'C
Cuts at: 5144 5144
Size: 7819
MfeI C'AATT_G
Cuts at: 5418 5418
Size: 7819
MluI A'CGCG_T
Cuts at: 7695 7695
Size: 7819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6933 7117 1305
Size: 5628 184 2007
Fragments arranged by size:
5628 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 4508 4692 10
Size: 54 1425 343 582 2094 184 3137
Fragments arranged by size:
3137 2094 1425 582 343 184 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2136 5777 6136 6295 518
Size: 648 970 3641 359 159 2042
Fragments arranged by size:
3641 2042 970 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2221 2336 2376 3030 3129 3172 3232
Size: 457 158 115 40 654 99 43 60
Cuts at: 3232 3753 3775 3856 3969 4047 4155 4201 4762
Size: 521 22 81 113 78 108 46 561
Cuts at: 4762 4822 4842 5235 5912 7060 7305 1606
Size: 60 20 393 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 561 521 457 393
245 158 115 113 108 99 81 78
60 60 46 43 40 22 20
NarI GG'CG_CC
Cuts at: 4543 4543
Size: 7819
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7267
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7819
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7819
NotI GC'GGCC_GC
Cuts at: 5266 5266
Size: 7819
NspI r_CATG'y
Cuts at: 1132 1229 5177 6722 1132
Size: 97 3948 1545 2229
Fragments arranged by size:
3948 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2173 2536 2787 3791 4215 4300 4556 1348
Size: 825 363 251 1004 424 85 256 4611
Fragments arranged by size:
4611 1004 825 424 363 256 251 85
PciI A'CATG_T
Cuts at: 6718 6718
Size: 7819
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3630 4079 1108
Size: 2522 449 4848
Fragments arranged by size:
4848 2522 449
PfoI T'CCnGG_A
Cuts at: 2923 3487 4577 5061 2923
Size: 564 1090 484 5681
Fragments arranged by size:
5681 1090 564 484
PmeI GTTT'AAAC
Cuts at: 5123 5123
Size: 7819
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3324 3356 5888 5920 7401 7433 3324
Size: 32 2532 32 1481 32 3710
Fragments arranged by size:
3710 2532 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2607 3050 3115 3445 4086 4518 4601 4658 5092
Size: 443 65 330 641 432 83 57 434
Cuts at: 5092 2607
Size: 5334
Fragments arranged by size:
5334 641 443 434 432 330 83 65
57
PshAI GACnn'nnGTC
Cuts at: 1613 2045 1613
Size: 432 7387
PsiI TTA'TAA
Cuts at: 5389 5389
Size: 7819
PspOMI G'GGCC_C
Cuts at: 2687 3133 4303 5055 2687
Size: 446 1170 752 5451
Fragments arranged by size:
5451 1170 752 446
PspXI vC'TCGA_Gb
Cuts at: 3958 3958
Size: 7819
PstI C_TGCA'G
Cuts at: 830 3360 3525 4154 4392 4422 4709 4808 4938
Size: 2530 165 629 238 30 287 99 130
Cuts at: 4938 4957 5171 830
Size: 19 214 3478
Fragments arranged by size:
3478 2530 629 287 238 214 165 130
99 30 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7819
PvuII CAG'CTG
Cuts at: 2063 2221 2336 2376 3030 3129 3232 3753 4047
Size: 158 115 40 654 99 103 521 294
Cuts at: 4047 4201 4822 4842 2063
Size: 154 621 20 5040
Fragments arranged by size:
5040 654 621 521 294 158 154 115
103 99 40 20
SacI G_AGCT'C
Cuts at: 2859 4008 2859
Size: 1149 6670
SalI G'TCGA_C
Cuts at: 1936 5161 1936
Size: 3225 4594
SanDI GG'GwC_CC
Cuts at: 3115 4086 4658 5092 3115
Size: 971 572 434 5842
Fragments arranged by size:
5842 971 572 434
SapI GCTCTTCn'nnn_
Cuts at: 2104 2576 4816 2104
Size: 472 2240 5107
Fragments arranged by size:
5107 2240 472
SbfI CC_TGCA'GG
Cuts at: 4938 5171 4938
Size: 233 7586
ScaI AGT'ACT
Cuts at: 6067 7779 6067
Size: 1712 6107
SfcI C'TryA_G
Cuts at: 826 1045 2912 3356 3521 4150 4388 4418 4705
Size: 219 1867 444 165 629 238 30 287
Cuts at: 4705 4804 4934 4953 5167 6302 6983 7174 826
Size: 99 130 19 214 1135 681 191 1471
Fragments arranged by size:
1867 1471 1135 681 629 444 287 238
219 214 191 165 130 99 30 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 3577 3577
Size: 7819
SfoI GGC'GCC
Cuts at: 4544 4544
Size: 7819
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7819
SmaI CCC'GGG
Cuts at: 1872 5146 1872
Size: 3274 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3144 3160 3542 3958 5923 6824
Size: 197 926 1201 16 382 416 1965 901
Cuts at: 6824 7086 820
Size: 262 1553
Fragments arranged by size:
1965 1553 1201 926 901 416 382 262
197 16
SnaBI TAC'GTA
Cuts at: 493 5116 5610 493
Size: 4623 494 2702
Fragments arranged by size:
4623 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7819
SphI G_CATG'C
Cuts at: 1132 5177 1132
Size: 4045 3774
SspI AAT'ATT
Cuts at: 5 52 5743 5
Size: 47 5691 2081
Fragments arranged by size:
5691 2081 47
StuI AGG'CCT
Cuts at: 1517 2367 2652 3665 4239 4491 4669 1517
Size: 850 285 1013 574 252 178 4667
Fragments arranged by size:
4667 1013 850 574 285 252 178
StyI C'CwwG_G
Cuts at: 513 1065 4549 5019 5258 513
Size: 552 3484 470 239 3074
Fragments arranged by size:
3484 3074 552 470 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 2816 3253 3622 4852 5846 5863
Size: 1255 996 240 437 369 1230 994 17
Cuts at: 5863 6016 6201 6646 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 996 994 445 437 369
240 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6065 7777 96
Size: 274 80 33 51 1443 4088 1712 138
Fragments arranged by size:
4088 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4854 4886 5057 5089 7779 7811 4854
Size: 32 171 32 2690 32 4862
Fragments arranged by size:
4862 2690 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2462 1316
Size: 1146 6673
XbaI T'CTAG_A
Cuts at: 5155 5155
Size: 7819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2764 2987 3388 2764
Size: 223 401 7195
Fragments arranged by size:
7195 401 223
XhoI C'TCGA_G
Cuts at: 1943 3958 1943
Size: 2015 5804
XmaI C'CCGG_G
Cuts at: 1870 5144 1870
Size: 3274 4545
XmnI GAAnn'nnTTC
Cuts at: 5948 5948
Size: 7819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5627 276
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PsrI RsrII SacII SexAI SrfI SwaI