Restriction map for
pFN21ASDA1859
(Circular) (Six-base) MAPSORT of: pFN21ASDA1859.seq Check: 949 from: 1 to: 6952
pFN21ASDA1859 6952 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 6952
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4295 1937
Size: 2358 4594
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2389 3362 3452 5015 6399 6667 1632
Size: 391 366 973 90 1563 1384 268 1917
Fragments arranged by size:
1917 1563 1384 973 391 366 268 90
AfeI AGC'GCT
Cuts at: 3948 3948
Size: 6952
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 5851 6828 1203
Size: 4648 977 1327
Fragments arranged by size:
4648 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 5681 5681
Size: 6952
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6952
AlwNI CAG_nnn'CTG
Cuts at: 2275 2951 2954 3005 4695 6267 2275
Size: 676 3 51 1690 1572 2960
Fragments arranged by size:
2960 1690 1572 676 51 3
ApaI G_GGCC'C
Cuts at: 2499 2636 2819 3913 3925 2499
Size: 137 183 1094 12 5526
Fragments arranged by size:
5526 1094 183 137 12
ApaLI G'TGCA_C
Cuts at: 5009 6165 5009
Size: 1156 5796
ApoI r'AATT_y
Cuts at: 1493 2135 3740 4261 4487 4639 6748 1493
Size: 642 1605 521 226 152 2109 1697
Fragments arranged by size:
2109 1697 1605 642 521 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2373 2567 3199 3825 4277 1870
Size: 73 430 194 632 626 452 4545
Fragments arranged by size:
4545 632 626 452 430 194 73
BamHI G'GATC_C
Cuts at: 2296 3423 4035 4233 4282 4649 2296
Size: 1127 612 198 49 367 4599
Fragments arranged by size:
4599 1127 612 367 198 49
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 3636 4130 4214 4273
Size: 325 217 627 372 1477 494 84 59
Cuts at: 4273 5728 618
Size: 1455 1842
Fragments arranged by size:
1842 1477 1455 627 494 372 325 217
84 59
BanII G_rGCy'C
Cuts at: 1391 2241 2499 2636 2819 3033 3172 3763 3913
Size: 850 258 137 183 214 139 591 150
Cuts at: 3913 3925 3990 4271 1391
Size: 12 65 281 4072
Fragments arranged by size:
4072 850 591 281 258 214 183 150
139 137 65 12
BbeI G_GCGC'C
Cuts at: 4218 4218
Size: 6952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2076 2719 928
Size: 971 177 643 5161
Fragments arranged by size:
5161 971 643 177
BbvCI CC'TCA_GC
Cuts at: 2028 3487 2028
Size: 1459 5493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3884 3918 5143 5177 1903
Size: 34 1947 34 1225 34 3678
Fragments arranged by size:
3678 1947 1225 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2452 4844 6054 2452
Size: 2392 1210 3350
Fragments arranged by size:
3350 2392 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2486 2570 2618
Size: 107 122 71 631 605 814 84 48
Cuts at: 2618 2801 2969 3584 5563 136
Size: 183 168 615 1979 1525
Fragments arranged by size:
1979 1525 814 631 615 605 183 168
122 107 84 71 48
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 3015 3027 4369 3015
Size: 12 1342 5598
Fragments arranged by size:
5598 1342 12
Bme1580I G_kGCm'C
Cuts at: 1790 2499 2636 2819 2841 3913 3925 5013 6169
Size: 709 137 183 22 1072 12 1088 1156
Cuts at: 6169 1790
Size: 2573
Fragments arranged by size:
2573 1156 1088 1072 709 183 137 22
12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6952
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2814 4683 5641
Size: 416 53 83 399 24 1397 1869 958
Cuts at: 5641 442
Size: 1753
Fragments arranged by size:
1869 1753 1397 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2424 1056
Size: 1368 5584
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3132 3132
Size: 6952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3157 5612 1239
Size: 1918 2455 2579
Fragments arranged by size:
2579 2455 1918
Bpu10I CC'TnA_GC
Cuts at: 2028 2490 2505 2985 3487 2028
Size: 462 15 480 502 5493
Fragments arranged by size:
5493 502 480 462 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3202 5077 5942 6240 3202
Size: 1875 865 298 3914
Fragments arranged by size:
3914 1875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2452 2776 3764 3813 5615 882
Size: 617 953 324 988 49 1802 2219
Fragments arranged by size:
2219 1802 988 953 617 324 49
BsaAI yAC'GTr
Cuts at: 493 1204 3226 4107 4249 4743 493
Size: 711 2022 881 142 494 2702
Fragments arranged by size:
2702 2022 881 711 494 142
BsaBI GATnn'nnATC
Cuts at: 3320 3932 6946 3320
Size: 612 3014 3326
Fragments arranged by size:
3326 3014 612
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2685 3552 4119 4215
Size: 53 83 186 936 1152 867 567 96
Cuts at: 4215 4759 5141 5776 275
Size: 544 382 635 1451
Fragments arranged by size:
1451 1152 936 867 635 567 544 382
186 96 83 53
BsaWI w'CCGG_w
Cuts at: 2299 4188 5385 5783 6057 6204 6615 2299
Size: 1889 1197 398 274 147 411 2636
Fragments arranged by size:
2636 1889 1197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6727
Fragments arranged by size:
6727 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3116 3119 3475 3524 1186
Size: 1930 3 356 49 4614
Fragments arranged by size:
4614 1930 356 49 3
BseYI C'CCAG_C
Cuts at: 2463 2696 2791 3966 4169 6155 6651 2463
Size: 233 95 1175 203 1986 496 2764
Fragments arranged by size:
2764 1986 1175 496 233 203 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3018 3457 1163
Size: 573 1282 439 4658
Fragments arranged by size:
4658 1282 573 439
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2325 4402 5163 6191 1540
Size: 275 186 324 2077 761 1028 2301
Fragments arranged by size:
2301 2077 1028 761 324 275 186
BsiHKAI G_wGCw'C
Cuts at: 3990 5013 5098 6169 3990
Size: 1023 85 1071 4773
Fragments arranged by size:
4773 1071 1023 85
BsmI GAATG_Cn'
Cuts at: 4463 4556 4463
Size: 93 6859
BsmBI CGTCTCn'nnnn_
Cuts at: 2312 2312
Size: 6952
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2499 2636 2819 2841 3033 3172
Size: 399 451 258 137 183 22 192 139
Cuts at: 3172 3763 3913 3925 3990 4271 5013 5098 6169
Size: 591 150 12 65 281 742 85 1071
Cuts at: 6169 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 591 451 399 281 258
192 183 150 139 137 85 65 22
12
BspEI T'CCGG_A
Cuts at: 2299 4188 6615 2299
Size: 1889 2427 2636
Fragments arranged by size:
2636 2427 1889
BspHI T'CATG_A
Cuts at: 3561 4841 6571 3561
Size: 1280 1730 3942
Fragments arranged by size:
3942 1730 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 2738 2794 4307 844
Size: 1590 304 56 1513 3489
Fragments arranged by size:
3489 1590 1513 304 56
BsrBI CCG'CTC
Cuts at: 1394 1823 4839 1394
Size: 429 3016 3507
Fragments arranged by size:
3507 3016 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2285 5447 5621 65
Size: 1183 1037 3162 174 1396
Fragments arranged by size:
3162 1396 1183 1037 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2691 2743 5596 5783 1700
Size: 110 881 52 2853 187 2869
Fragments arranged by size:
2869 2853 881 187 110 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6952
BssHII G'CGCG_C
Cuts at: 1925 3328 1925
Size: 1403 5549
BssSI C'ACGA_G
Cuts at: 2974 5012 6024 2974
Size: 2038 1012 3902
Fragments arranged by size:
3902 2038 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2951 2954 3005 794
Size: 2157 3 51 4741
Fragments arranged by size:
4741 2157 51 3
BstBI TT'CG_AA
Cuts at: 2007 5756 2007
Size: 3749 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6952
BstXI CCAn_nnnn'nTGG
Cuts at: 2557 2680 3076 3135 2557
Size: 123 396 59 6374
Fragments arranged by size:
6374 396 123 59
BstYI r'GATC_y
Cuts at: 1972 2296 3315 3423 3711 4035 4233 4282 4649
Size: 324 1019 108 288 324 198 49 367
Cuts at: 4649 5034 5051 6503 6589 6601 6947 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1019 385 367 346 324 324
288 198 108 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3120 513
Size: 552 957 1098 4345
Fragments arranged by size:
4345 1098 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3784 4035 487
Size: 683 56 2558 251 3404
Fragments arranged by size:
3404 2558 683 251 56
BtsI GCAGTG_nn'
Cuts at: 3009 4132 4468 5262 5282 3009
Size: 1123 336 794 20 4679
Fragments arranged by size:
4679 1123 794 336 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3864 3899 529
Size: 35 3300 35 3582
Fragments arranged by size:
3582 3300 35 35
DraI TTT'AAA
Cuts at: 4256 4256
Size: 6952
DraIII CAC_nnn'GTG
Cuts at: 2431 2431
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2890 3064 3980 5959 809
Size: 507 1574 174 916 1979 1802
Fragments arranged by size:
1979 1802 1574 916 507 174
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2433 2907
Size: 54 1425 50 275 18 492 111 474
Cuts at: 2907 2970 3891 4399 5288 6917 8
Size: 63 921 508 889 1629 43
Fragments arranged by size:
1629 1425 921 889 508 492 474 275
111 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2322 4399 1537
Size: 275 510 2077 4090
Fragments arranged by size:
4090 2077 510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 4882 1353
Size: 747 2782 3423
Fragments arranged by size:
3423 2782 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5539 5913 6059 387
Size: 1064 138 3950 374 146 1280
Fragments arranged by size:
3950 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3988 3988
Size: 6952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2389 3157 3362 3452 5015 5612
Size: 393 391 366 768 205 90 1563 597
Cuts at: 5612 6399 6667 1239
Size: 787 268 1524
Fragments arranged by size:
1563 1524 787 768 597 393 391 366
268 205 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 3037 3260 3596 4030 2279
Size: 758 223 336 434 5201
Fragments arranged by size:
5201 758 434 336 223
EcoO109I rG'GnC_Cy
Cuts at: 2495 2632 2633 2815 2816 2849 3204 3751 3787
Size: 137 1 182 1 33 355 547 36
Cuts at: 3787 3909 3921 2495
Size: 122 12 5526
Fragments arranged by size:
5526 547 355 182 137 122 36 33
12 1 1
EcoRI G'AATT_C
Cuts at: 2135 3740 4261 4639 2135
Size: 1605 521 378 4448
Fragments arranged by size:
4448 1605 521 378
FspI TGC'GCA
Cuts at: 5458 5458
Size: 6952
HaeII r_GCGC'y
Cuts at: 1678 2646 2906 3950 4218 6099 1678
Size: 968 260 1044 268 1881 2531
Fragments arranged by size:
2531 1881 1044 968 268 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4104 4136 5593 5625 5667
Size: 32 135 32 2532 32 1457 32 42
Cuts at: 5667 5699 1373
Size: 32 2626
Fragments arranged by size:
2626 2532 1457 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4296 4542 669
Size: 988 281 2358 246 3079
Fragments arranged by size:
3079 2358 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2035 2203 748
Size: 1287 168 5497
Fragments arranged by size:
5497 1287 168
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KasI G'GCGC_C
Cuts at: 4214 4214
Size: 6952
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 6066 6250 1305
Size: 1080 3681 184 2007
Fragments arranged by size:
3681 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 2909 2972 10
Size: 54 1425 343 603 474 63 3990
Fragments arranged by size:
3990 1425 603 474 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3078 3560 4910 5269 5428 518
Size: 648 1912 482 1350 359 159 2042
Fragments arranged by size:
2042 1912 1350 648 482 359 159
MspA1I CmG'CkG
Cuts at: 1606 2293 2381 2390 2951 3002 3122 4368 5045
Size: 687 88 9 561 51 120 1246 677
Cuts at: 5045 6193 6438 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1246 1148 687 677 561 245 120
88 51 9
NaeI GCC'GGC
Cuts at: 2745 2745
Size: 6952
NarI GG'CG_CC
Cuts at: 4215 4215
Size: 6952
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5443
Fragments arranged by size:
5443 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NgoMIV G'CCGG_C
Cuts at: 2743 2743
Size: 6952
NheI G'CTAG_C
Cuts at: 1052 2420 1052
Size: 1368 5584
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 3340 3905 4310 5855 1132
Size: 97 2111 565 405 1545 2229
Fragments arranged by size:
2229 2111 1545 565 405 97
PasI CC'CwG_GG
Cuts at: 1348 2241 3033 3626 1348
Size: 893 792 593 4674
Fragments arranged by size:
4674 893 792 593
PciI A'CATG_T
Cuts at: 5851 5851
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 2431 1108
Size: 1167 156 5629
Fragments arranged by size:
5629 1167 156
PfoI T'CCnGG_A
Cuts at: 4229 4229
Size: 6952
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PmlI CAC'GTG
Cuts at: 4107 4107
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 6534 6566 5021
Size: 32 1481 32 5407
Fragments arranged by size:
5407 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2849 3204 3787 2849
Size: 355 583 6014
Fragments arranged by size:
6014 583 355
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PspOMI G'GGCC_C
Cuts at: 2495 2632 2815 3909 3921 2495
Size: 137 183 1094 12 5526
Fragments arranged by size:
5526 1094 183 137 12
PstI C_TGCA'G
Cuts at: 830 3264 4304 830
Size: 2434 1040 3478
Fragments arranged by size:
3478 2434 1040
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 2293 2951 3002 2293
Size: 658 51 6243
Fragments arranged by size:
6243 658 51
SacI G_AGCT'C
Cuts at: 3990 3990
Size: 6952
SacII CC_GC'GG
Cuts at: 3123 3123
Size: 6952
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SfcI C'TryA_G
Cuts at: 826 1045 2059 3260 4300 5435 6116 6307 826
Size: 219 1014 1201 1040 1135 681 191 1471
Fragments arranged by size:
1471 1201 1135 1040 1014 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2969 2969
Size: 6952
SfoI GGC'GCC
Cuts at: 4216 4216
Size: 6952
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 3827 4279 1872
Size: 1955 452 4545
Fragments arranged by size:
4545 1955 452
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3217 5056 5957 6219 820
Size: 197 926 1274 1839 901 262 1553
Fragments arranged by size:
1839 1553 1274 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4249 4743 493
Size: 3756 494 2702
Fragments arranged by size:
3756 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 4310 1132
Size: 3178 3774
SspI AAT'ATT
Cuts at: 5 52 4876 5
Size: 47 4824 2081
Fragments arranged by size:
4824 2081 47
StuI AGG'CCT
Cuts at: 1517 2217 1517
Size: 700 6252
StyI C'CwwG_G
Cuts at: 513 1065 2022 2958 3499 3510 4391 513
Size: 552 957 936 541 11 881 3074
Fragments arranged by size:
3074 957 936 881 552 541 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2911 3145 3654 4979 4996 5149 5334
Size: 1255 1331 234 509 1325 17 153 185
Cuts at: 5334 5779 325
Size: 445 1498
Fragments arranged by size:
1498 1331 1325 1255 509 445 234 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4068 5198 6910
Size: 274 80 33 51 1443 2091 1130 1712
Cuts at: 6910 96
Size: 138
Fragments arranged by size:
2091 1712 1443 1130 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6912 6944 6912
Size: 32 6920
Tth111I GACn'n_nGTC
Cuts at: 1316 2093 1316
Size: 777 6175
XbaI T'CTAG_A
Cuts at: 4288 4288
Size: 6952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2965 2849
Size: 116 6836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 3825 4277 1870
Size: 1955 452 4545
Fragments arranged by size:
4545 1955 452
XmnI GAAnn'nnTTC
Cuts at: 5081 5081
Size: 6952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SanDI SapI SexAI SrfI SwaI