Restriction map for
pFN21ASDA1813
(Circular) (Six-base) MAPSORT of: pFN21ASDA1813.seq Check: 5713 from: 1 to: 6736
pFN21ASDA1813 6736 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2769 3190 3661 3838 2769
Size: 421 471 177 5667
Fragments arranged by size:
5667 471 421 177
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3505 4079 1937
Size: 1568 574 4594
Fragments arranged by size:
4594 1568 574
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2595 3026 3401 4799 6183 6451 1632
Size: 456 507 431 375 1398 1384 268 1917
Fragments arranged by size:
1917 1398 1384 507 456 431 375 268
AfeI AGC'GCT
Cuts at: 2951 2951
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 5635 6612 1203
Size: 4432 977 1327
Fragments arranged by size:
4432 1327 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3630 1184
Size: 2446 4290
AlwNI CAG_nnn'CTG
Cuts at: 2041 2618 2655 2940 3049 3190 3256 3391 3559
Size: 577 37 285 109 141 66 135 168
Cuts at: 3559 3925 4479 6051 2041
Size: 366 554 1572 2726
Fragments arranged by size:
2726 1572 577 554 366 285 168 141
135 109 66 37
ApaI G_GGCC'C
Cuts at: 2181 2579 2826 3181 3742 2181
Size: 398 247 355 561 5175
Fragments arranged by size:
5175 561 398 355 247
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 4045 4271 4423 6532 1493
Size: 2552 226 152 2109 1697
Fragments arranged by size:
2552 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2352 2487 2523 2805 2916 3317
Size: 73 195 214 135 36 282 111 401
Cuts at: 3317 3519 3525 3954 4061 1870
Size: 202 6 429 107 4545
Fragments arranged by size:
4545 429 401 282 214 202 195 135
111 107 73 36 6
AvrII C'CTAG_G
Cuts at: 2258 2875 2258
Size: 617 6119
BamHI G'GATC_C
Cuts at: 4066 4433 4066
Size: 367 6369
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 2757 4057 5512 618
Size: 325 217 627 939 31 1300 1455 1842
Fragments arranged by size:
1842 1455 1300 939 627 325 217 31
BanII G_rGCy'C
Cuts at: 1391 2181 2497 2579 2826 3181 3318 3526 3642
Size: 790 316 82 247 355 137 208 116
Cuts at: 3642 3742 3963 4055 1391
Size: 100 221 92 4072
Fragments arranged by size:
4072 790 355 316 247 221 208 137
116 100 92 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5765
BbvCI CC'TCA_GC
Cuts at: 3618 3618
Size: 6736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4927 4961 1903
Size: 34 2990 34 3678
Fragments arranged by size:
3678 2990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2166 3833 3986
Size: 107 122 71 631 605 494 1667 153
Cuts at: 3986 5347 136
Size: 1361 1525
Fragments arranged by size:
1667 1525 1361 631 605 494 153 122
107 71
BglII A'GATC_T
Cuts at: 3369 4026 6731 3369
Size: 657 2705 3374
Fragments arranged by size:
3374 2705 657
BlpI GC'TnA_GC
Cuts at: 3582 3935 4153 3582
Size: 353 218 6165
Fragments arranged by size:
6165 353 218
Bme1580I G_kGCm'C
Cuts at: 1790 2181 2246 2256 2368 2579 2760 2791 2826
Size: 391 65 10 112 211 181 31 35
Cuts at: 2826 3172 3181 3742 4797 5953 1790
Size: 346 9 561 1055 1156 2573
Fragments arranged by size:
2573 1156 1055 561 391 346 211 181
112 65 35 31 10 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 4467 5425
Size: 416 53 83 399 24 1412 1638 958
Cuts at: 5425 442
Size: 1753
Fragments arranged by size:
1753 1638 1412 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2076 2444 2839 3296 3554 3962 3968
Size: 836 1 368 395 457 258 408 6
Cuts at: 3968 4025 5396 1239
Size: 57 1371 2579
Fragments arranged by size:
2579 1371 836 457 408 395 368 258
57 6 1
Bpu10I CC'TnA_GC
Cuts at: 3618 3618
Size: 6736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3350 4861 5726 6024 3350
Size: 1511 865 298 4062
Fragments arranged by size:
4062 1511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3018 3329 5399 882
Size: 617 1519 311 2070 2219
Fragments arranged by size:
2219 2070 1519 617 311
BsaAI yAC'GTr
Cuts at: 493 1204 4033 4527 493
Size: 711 2829 494 2702
Fragments arranged by size:
2829 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2447 6730 2447
Size: 4283 2453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4543 4925 5560 275
Size: 53 83 186 936 3010 382 635 1451
Fragments arranged by size:
3010 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2307 2720 5169 5567 5841 5988 6399 2307
Size: 413 2449 398 274 147 411 2644
Fragments arranged by size:
2644 2449 413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2921 2951 3326
Size: 30 165 30 822 30 666 30 375
Cuts at: 3326 3356 1178
Size: 30 4558
Fragments arranged by size:
4558 822 666 375 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2575 2578 2581 2584 2593 2596 2842
Size: 956 433 3 3 3 9 3 246
Cuts at: 2842 3113 3347 3806 4022 1186
Size: 271 234 459 216 3900
Fragments arranged by size:
3900 956 459 433 271 246 234 216
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2245 2324 2643 2891 3602 5939 6435 2245
Size: 79 319 248 711 2337 496 2546
Fragments arranged by size:
2546 2337 711 496 319 248 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2981 3437 3722 1163
Size: 573 318 927 456 285 4177
Fragments arranged by size:
4177 927 573 456 318 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3627 4186 4947 5975 1540
Size: 275 186 1626 559 761 1028 2301
Fragments arranged by size:
2301 1626 1028 761 559 275 186
BsiHKAI G_wGCw'C
Cuts at: 2867 3318 3526 3963 4797 4882 5953 2867
Size: 451 208 437 834 85 1071 3650
Fragments arranged by size:
3650 1071 834 451 437 208 85
BsmI GAATG_Cn'
Cuts at: 4247 4340 4247
Size: 93 6643
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2246 2256 2368 2497 2579 2760
Size: 399 391 65 10 112 129 82 181
Cuts at: 2760 2791 2826 2867 3172 3181 3318 3526 3642
Size: 31 35 41 305 9 137 208 116
Cuts at: 3642 3742 3963 4055 4797 4882 5953 1391
Size: 100 221 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 305 221 208
181 137 129 116 112 100 92 85
82 65 41 35 31 10 9
BspEI T'CCGG_A
Cuts at: 2307 6399 2307
Size: 4092 2644
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2769 3190 3661 3838 4091 844
Size: 1925 421 471 177 253 3489
Fragments arranged by size:
3489 1925 471 421 253 177
BsrBI CCG'CTC
Cuts at: 1394 1823 3103 3280 3301 3778 3820 4623 1394
Size: 429 1280 177 21 477 42 803 3507
Fragments arranged by size:
3507 1280 803 477 429 177 42 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 5231 5405 65
Size: 1183 3983 174 1396
Fragments arranged by size:
3983 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2127 2844 3897 3906 5380 5567 1700
Size: 110 317 717 1053 9 1474 187 2869
Fragments arranged by size:
2869 1474 1053 717 317 187 110 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 4796 5808 4796
Size: 1012 5724
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 2372 3925 794
Size: 1247 331 1553 3605
Fragments arranged by size:
3605 1553 1247 331
BstBI TT'CG_AA
Cuts at: 2007 5540 2007
Size: 3533 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstXI CCAn_nnnn'nTGG
Cuts at: 2201 2777 2201
Size: 576 6160
BstYI r'GATC_y
Cuts at: 1972 2442 3369 4026 4066 4433 4818 4835 6287
Size: 470 927 657 40 367 385 17 1452
Cuts at: 6287 6373 6385 6731 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 927 657 470 385 367 346
86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2164 2383 2770 513
Size: 552 957 142 219 387 4479
Fragments arranged by size:
4479 957 552 387 219 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5997
Fragments arranged by size:
5997 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2341 2789 2914 4014 4252 5046 5066 2076
Size: 265 448 125 1100 238 794 20 3746
Fragments arranged by size:
3746 1100 794 448 265 238 125 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2683 2842
Size: 54 1425 50 275 18 195 658 159
Cuts at: 2842 3624 3978 4183 5072 6701 8
Size: 782 354 205 889 1629 43
Fragments arranged by size:
1629 1425 889 782 658 354 275 205
195 159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3624 4183 1537
Size: 275 1812 559 4090
Fragments arranged by size:
4090 1812 559 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2602 2614 4666 1353
Size: 1249 12 2052 3423
Fragments arranged by size:
3423 2052 1249 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2180 2441 3695 5323 5697 5843
Size: 1064 138 591 261 1254 1628 374 146
Cuts at: 5843 387
Size: 1280
Fragments arranged by size:
1628 1280 1254 1064 591 374 261 146
138
EcoICRI GAG'CTC
Cuts at: 3316 3524 3961 3316
Size: 208 437 6091
Fragments arranged by size:
6091 437 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2076 2088 2444 2595 2839 3026
Size: 393 443 1 12 356 151 244 187
Cuts at: 3026 3296 3401 3554 3962 3968 4025 4799 5396
Size: 270 105 153 408 6 57 774 597
Cuts at: 5396 6183 6451 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 774 597 443 408 393 356
270 268 244 187 153 151 105 57
12 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3140 3140
Size: 6736
EcoO109I rG'GnC_Cy
Cuts at: 2519 2575 2576 2812 2823 2989 3133 3988 2519
Size: 56 1 236 11 166 144 855 5267
Fragments arranged by size:
5267 855 236 166 144 56 11 1
EcoRI G'AATT_C
Cuts at: 4045 4423 4045
Size: 378 6358
FseI GG_CCGG'CC
Cuts at: 2131 2131
Size: 6736
FspI TGC'GCA
Cuts at: 5242 5242
Size: 6736
HaeII r_GCGC'y
Cuts at: 1678 2953 3286 3455 5883 1678
Size: 1275 333 169 2428 2531
Fragments arranged by size:
2531 2428 1275 333 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 5377 5409 5451
Size: 32 135 32 1349 32 2424 32 42
Cuts at: 5451 5483 1373
Size: 32 2626
Fragments arranged by size:
2626 2424 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4080 4326 669
Size: 988 281 2142 246 3079
Fragments arranged by size:
3079 2142 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6736
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 3230 4335 3230
Size: 1105 5631
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 3591 5850 6034 1305
Size: 1395 891 2259 184 2007
Fragments arranged by size:
2259 2007 1395 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 10
Size: 54 1425 343 195 4719
Fragments arranged by size:
4719 1425 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3890 4694 5053 5212 518
Size: 648 2724 804 359 159 2042
Fragments arranged by size:
2724 2042 804 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2239 2249 2362 2385 2566 2615 3049 3109
Size: 633 10 113 23 181 49 434 60
Cuts at: 3109 3166 3175 3187 3196 3211 3253 3274 3310
Size: 57 9 12 9 15 42 21 36
Cuts at: 3310 3391 3589 3662 3706 3772 3799 3871 4152
Size: 81 198 73 44 66 27 72 281
Cuts at: 4152 4829 5977 6222 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 434 281 245 198
181 113 81 73 72 66 60 57
49 44 42 36 27 23 21 15
12 10 9 9
NaeI GCC'GGC
Cuts at: 2129 2129
Size: 6736
NcoI C'CATG_G
Cuts at: 513 1065 2022 2770 513
Size: 552 957 748 4479
Fragments arranged by size:
4479 957 748 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NgoMIV G'CCGG_C
Cuts at: 2127 2127
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 4094 5639 1132
Size: 97 2865 1545 2229
Fragments arranged by size:
2865 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6736
PciI A'CATG_T
Cuts at: 5635 5635
Size: 6736
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6736
PfoI T'CCnGG_A
Cuts at: 3006 3633 3006
Size: 627 6109
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2519 2812 2519
Size: 293 6443
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PspOMI G'GGCC_C
Cuts at: 2177 2575 2822 3177 3738 2177
Size: 398 247 355 561 5175
Fragments arranged by size:
5175 561 398 355 247
PspXI vC'TCGA_Gb
Cuts at: 2487 2487
Size: 6736
PstI C_TGCA'G
Cuts at: 830 3195 3201 3216 3261 3564 3771 3876 3930
Size: 2365 6 15 45 303 207 105 54
Cuts at: 3930 4088 830
Size: 158 3478
Fragments arranged by size:
3478 2365 303 207 158 105 54 45
15 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2249 2566 2615 3049 3187 3196 3211 3253 3274
Size: 317 49 434 138 9 15 42 21
Cuts at: 3274 3391 3589 3871 2249
Size: 117 198 282 5114
Fragments arranged by size:
5114 434 317 282 198 138 117 49
42 21 15 9
SacI G_AGCT'C
Cuts at: 3318 3526 3963 3318
Size: 208 437 6091
Fragments arranged by size:
6091 437 208
SacII CC_GC'GG
Cuts at: 2386 2386
Size: 6736
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SanDI GG'GwC_CC
Cuts at: 2812 2812
Size: 6736
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 2713 3191 3197 3212 3257 3506 3560
Size: 219 1668 478 6 15 45 249 54
Cuts at: 3560 3767 3872 3926 4084 5219 5900 6091 826
Size: 207 105 54 158 1135 681 191 1471
Fragments arranged by size:
1668 1471 1135 681 478 249 219 207
191 158 105 54 54 45 15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 3986 3986
Size: 6736
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 2140 3956 4063 1872
Size: 268 1816 107 4545
Fragments arranged by size:
4545 1816 268 107
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2487 3329 4840 5741 6003 820
Size: 197 926 544 842 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 842 544 262 197
SnaBI TAC'GTA
Cuts at: 493 4033 4527 493
Size: 3540 494 2702
Fragments arranged by size:
3540 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6736
StyI C'CwwG_G
Cuts at: 513 1065 2022 2258 2770 2875 3294 4175 513
Size: 552 957 236 512 105 419 881 3074
Fragments arranged by size:
3074 957 881 552 512 419 236 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4763 4780 4933 5118 5563 325
Size: 1255 3183 17 153 185 445 1498
Fragments arranged by size:
3183 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4982 6694 96
Size: 274 80 33 51 1443 3005 1712 138
Fragments arranged by size:
3005 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 2750 2782 3326 3358 6696 6728 2225
Size: 32 493 32 544 32 3338 32 2233
Fragments arranged by size:
3338 2233 544 493 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2314 1316
Size: 998 5738
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2837 2171
Size: 666 6070
XhoI C'TCGA_G
Cuts at: 1943 2487 1943
Size: 544 6192
XmaI C'CCGG_G
Cuts at: 1870 2138 3954 4061 1870
Size: 268 1816 107 4545
Fragments arranged by size:
4545 1816 268 107
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FspAI KasI NarI
NruI NsiI PacI PmlI PsrI RsrII SapI SexAI
SfoI SrfI SwaI