Restriction map for
pFN21ASDA1810
(Circular) (Six-base) MAPSORT of: pFN21ASDA1810.seq Check: 6103 from: 1 to: 6424
pFN21ASDA1810 6424 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2861 2861
Size: 6424
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4234 278
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3767 1937
Size: 1830 4594
Acc65I G'GTAC_C
Cuts at: 3745 3745
Size: 6424
AclI AA'CG_TT
Cuts at: 1563 2260 4551 4924 1563
Size: 697 2291 373 3063
Fragments arranged by size:
3063 2291 697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 3465 4487 5871 6139 1632
Size: 670 1163 1022 1384 268 1917
Fragments arranged by size:
1917 1384 1163 1022 670 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6227
AflIII A'CryG_T
Cuts at: 1203 5323 6300 1203
Size: 4120 977 1327
Fragments arranged by size:
4120 1327 977
AgeI A'CCGG_T
Cuts at: 5255 5255
Size: 6424
AhdI GACnn_n'nnGTC
Cuts at: 5153 5153
Size: 6424
AleI CACnn'nnGTG
Cuts at: 1166 2036 2120 1166
Size: 870 84 5470
Fragments arranged by size:
5470 870 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6424
AlwNI CAG_nnn'CTG
Cuts at: 3143 3179 3191 3209 3364 3385 3593 3617 3697
Size: 36 12 18 155 21 208 24 80
Cuts at: 3697 4167 5739 3143
Size: 470 1572 3828
Fragments arranged by size:
3828 1572 470 208 155 80 36 24
21 18 12
ApaI G_GGCC'C
Cuts at: 3038 3465 3038
Size: 427 5997
ApaLI G'TGCA_C
Cuts at: 2579 4481 5637 2579
Size: 1902 1156 3366
Fragments arranged by size:
3366 1902 1156
ApoI r'AATT_y
Cuts at: 1493 3733 3959 4111 6220 1493
Size: 2240 226 152 2109 1697
Fragments arranged by size:
2240 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4978 160
Size: 872 3946 1606
Fragments arranged by size:
3946 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
AvaI C'yCGr_G
Cuts at: 1870 1943 3749 1870
Size: 73 1806 4545
Fragments arranged by size:
4545 1806 73
BamHI G'GATC_C
Cuts at: 3754 4121 3754
Size: 367 6057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2771 3146 3681 3745 5200
Size: 325 217 627 984 375 535 64 1455
Cuts at: 5200 618
Size: 1842
Fragments arranged by size:
1842 1455 984 627 535 375 325 217
64
BanII G_rGCy'C
Cuts at: 1391 2721 3038 3340 3465 3660 3675 3743 1391
Size: 1330 317 302 125 195 15 68 4072
Fragments arranged by size:
4072 1330 317 302 195 125 68 15
BbeI G_GCGC'C
Cuts at: 3685 3685
Size: 6424
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2176 928
Size: 971 277 5176
Fragments arranged by size:
5176 971 277
BbvCI CC'TCA_GC
Cuts at: 2382 2961 2382
Size: 579 5845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4615 4649 1903
Size: 34 2678 34 3678
Fragments arranged by size:
3678 2678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2706 4316 5526 2706
Size: 1610 1210 3604
Fragments arranged by size:
3604 1610 1210
BclI T'GATC_A
Cuts at: 1548 2571 1548
Size: 1023 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 3206 3469
Size: 107 122 71 631 605 385 1149 263
Cuts at: 3469 5035 136
Size: 1566 1525
Fragments arranged by size:
1566 1525 1149 631 605 385 263 122
107 71
BglII A'GATC_T
Cuts at: 6419 6419
Size: 6424
BlpI GC'TnA_GC
Cuts at: 3841 3841
Size: 6424
Bme1580I G_kGCm'C
Cuts at: 1790 2583 3038 3297 3465 3632 4485 5641 1790
Size: 793 455 259 168 167 853 1156 2573
Fragments arranged by size:
2573 1156 853 793 455 259 168 167
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6424
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4155 5113
Size: 416 53 83 399 24 1694 1044 958
Cuts at: 5113 442
Size: 1753
Fragments arranged by size:
1753 1694 1044 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2705 2988 3362 3386 3594 3719 5084
Size: 1211 255 283 374 24 208 125 1365
Cuts at: 5084 1239
Size: 2579
Fragments arranged by size:
2579 1365 1211 374 283 255 208 125
24
Bpu10I CC'TnA_GC
Cuts at: 2382 2961 3067 2382
Size: 579 106 5739
Fragments arranged by size:
5739 579 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4549 5414 5712 4549
Size: 865 298 5261
Fragments arranged by size:
5261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5087 882
Size: 617 3588 2219
Fragments arranged by size:
3588 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3721 4215 493
Size: 711 2517 494 2702
Fragments arranged by size:
2702 2517 711 494
BsaBI GATnn'nnATC
Cuts at: 6418 6418
Size: 6424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3411 3682 4231 4613
Size: 53 83 186 936 1878 271 549 382
Cuts at: 4613 5248 275
Size: 635 1451
Fragments arranged by size:
1878 1451 936 635 549 382 271 186
83 53
BsaWI w'CCGG_w
Cuts at: 4857 5255 5529 5676 6087 4857
Size: 398 274 147 411 5194
Fragments arranged by size:
5194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3063 3093 1178
Size: 30 165 30 1660 30 4509
Fragments arranged by size:
4509 1660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 3075 3228 3341 3512 3520 3650 1186
Size: 1543 346 153 113 171 8 130 3960
Fragments arranged by size:
3960 1543 346 171 153 130 113 8
BseYI C'CCAG_C
Cuts at: 2144 3062 3311 5627 6123 2144
Size: 918 249 2316 496 2445
Fragments arranged by size:
2445 2316 918 496 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 3120 1163
Size: 573 699 685 4467
Fragments arranged by size:
4467 699 685 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3874 4635 5663 1540
Size: 275 186 1873 761 1028 2301
Fragments arranged by size:
2301 1873 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 2427 2583 2721 3340 3675 4485 4570 5641
Size: 177 156 138 619 335 810 85 1071
Cuts at: 5641 2250
Size: 3033
Fragments arranged by size:
3033 1071 810 619 335 177 156 138
85
BsmI GAATG_Cn'
Cuts at: 3935 4028 3935
Size: 93 6331
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2427 2583 2721 3038 3297 3340
Size: 399 460 177 156 138 317 259 43
Cuts at: 3340 3465 3632 3660 3675 3743 4485 4570 5641
Size: 125 167 28 15 68 742 85 1071
Cuts at: 5641 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 460 399 317 259 177
167 156 138 125 85 68 43 28
15
BspEI T'CCGG_A
Cuts at: 6087 6087
Size: 6424
BspHI T'CATG_A
Cuts at: 2559 2568 4313 6043 2559
Size: 9 1745 1730 2940
Fragments arranged by size:
2940 1745 1730 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 2861 3779 844
Size: 2008 9 918 3489
Fragments arranged by size:
3489 2008 918 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3355 4311 1394
Size: 429 1532 956 3507
Fragments arranged by size:
3507 1532 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2733 4919 5093 65
Size: 1183 1485 2186 174 1396
Fragments arranged by size:
2186 1485 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3678 5068 5255 1700
Size: 110 1868 1390 187 2869
Fragments arranged by size:
2869 1868 1390 187 110
BsrGI T'GTAC_A
Cuts at: 96 2160 2499 2547 96
Size: 2064 339 48 3973
Fragments arranged by size:
3973 2064 339 48
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6424
BssSI C'ACGA_G
Cuts at: 4484 5496 4484
Size: 1012 5412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2748 3385 794
Size: 1954 637 3833
Fragments arranged by size:
3833 1954 637
BstBI TT'CG_AA
Cuts at: 2007 5228 2007
Size: 3221 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6424
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 2617
Size: 6424
BstYI r'GATC_y
Cuts at: 1972 3754 4121 4506 4523 5975 6061 6073 6419
Size: 1782 367 385 17 1452 86 12 346
Cuts at: 6419 1972
Size: 1977
Fragments arranged by size:
1977 1782 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3645 3645
Size: 6424
BtgI C'CryG_G
Cuts at: 513 1065 2457 3030 513
Size: 552 1392 573 3907
Fragments arranged by size:
3907 1392 573 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2862 487
Size: 683 56 1636 4049
Fragments arranged by size:
4049 1636 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 3401 3940 4734 4754 2043
Size: 1358 539 794 20 3713
Fragments arranged by size:
3713 1358 794 539 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2937 2972 3496 3531 529
Size: 35 2373 35 524 35 3422
Fragments arranged by size:
3422 2373 524 35 35 35
DraI TTT'AAA
Cuts at: 3728 3728
Size: 6424
DraIII CAC_nnn'GTG
Cuts at: 2458 2458
Size: 6424
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5431 809
Size: 507 4115 1802
Fragments arranged by size:
4115 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2619 3871
Size: 54 1425 50 275 18 219 570 1252
Cuts at: 3871 4760 6389 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1252 889 570 275 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3871 1537
Size: 275 2059 4090
Fragments arranged by size:
4090 2059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 2352 2360 2751 4354 1353
Size: 992 7 8 391 1603 3423
Fragments arranged by size:
3423 1603 992 391 8 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3665 5011 5385 5531 387
Size: 1064 138 2076 1346 374 146 1280
Fragments arranged by size:
2076 1346 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2719 3338 3673 2719
Size: 619 335 5470
Fragments arranged by size:
5470 619 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2450 2705 2988 3362 3386 3465
Size: 393 670 148 255 283 374 24 79
Cuts at: 3465 3594 3719 4487 5084 5871 6139 1239
Size: 129 125 768 597 787 268 1524
Fragments arranged by size:
1524 787 768 670 597 393 374 283
268 255 148 129 125 79 24
EcoO109I rG'GnC_Cy
Cuts at: 2983 3035 3461 3462 2983
Size: 52 426 1 5945
Fragments arranged by size:
5945 426 52 1
EcoRI G'AATT_C
Cuts at: 3733 4111 3733
Size: 378 6046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 6424
FspI TGC'GCA
Cuts at: 4930 4930
Size: 6424
HaeII r_GCGC'y
Cuts at: 1678 2063 3685 5571 1678
Size: 385 1622 1886 2531
Fragments arranged by size:
2531 1886 1622 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5065 5097 5139 5171 1373
Size: 32 135 32 3493 32 42 32 2626
Fragments arranged by size:
3493 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3487 3768 4014 669
Size: 988 281 1549 281 246 3079
Fragments arranged by size:
3079 1549 988 281 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6424
HpaI GTT'AAC
Cuts at: 4014 4014
Size: 6424
KasI G'GCGC_C
Cuts at: 3681 3681
Size: 6424
KpnI G_GTAC'C
Cuts at: 3749 3749
Size: 6424
MfeI C'AATT_G
Cuts at: 4023 4023
Size: 6424
MluI A'CGCG_T
Cuts at: 6300 6300
Size: 6424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5538 5722 1305
Size: 4233 184 2007
Fragments arranged by size:
4233 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 10
Size: 54 1425 343 789 3813
Fragments arranged by size:
3813 1425 789 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2120 2411 4382 4741 4900 518
Size: 648 870 84 291 1971 359 159 2042
Fragments arranged by size:
2042 1971 870 648 359 291 159 84
MspA1I CmG'CkG
Cuts at: 1606 2811 2833 3140 3840 4517 5665 5910 1606
Size: 1205 22 307 700 677 1148 245 2120
Fragments arranged by size:
2120 1205 1148 700 677 307 245 22
NaeI GCC'GGC
Cuts at: 3680 3680
Size: 6424
NarI GG'CG_CC
Cuts at: 3682 3682
Size: 6424
NcoI C'CATG_G
Cuts at: 513 1065 3030 513
Size: 552 1965 3907
Fragments arranged by size:
3907 1965 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6424
NgoMIV G'CCGG_C
Cuts at: 3678 3678
Size: 6424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6424
NotI GC'GGCC_GC
Cuts at: 3871 3871
Size: 6424
NspI r_CATG'y
Cuts at: 1132 1229 2506 3013 3782 5327 1132
Size: 97 1277 507 769 1545 2229
Fragments arranged by size:
2229 1545 1277 769 507 97
PasI CC'CwG_GG
Cuts at: 1348 2481 2980 1348
Size: 1133 499 4792
Fragments arranged by size:
4792 1133 499
PciI A'CATG_T
Cuts at: 5323 5323
Size: 6424
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2728 3191 3209 1108
Size: 1269 351 463 18 4323
Fragments arranged by size:
4323 1269 463 351 18
PmeI GTTT'AAAC
Cuts at: 3728 3728
Size: 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 6006 6038 4493
Size: 32 1481 32 4879
Fragments arranged by size:
4879 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6424
PsiI TTA'TAA
Cuts at: 3994 3994
Size: 6424
PspOMI G'GGCC_C
Cuts at: 3034 3461 3034
Size: 427 5997
PstI C_TGCA'G
Cuts at: 830 3381 3426 3693 3776 830
Size: 2551 45 267 83 3478
Fragments arranged by size:
3478 2551 267 83 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6424
PvuII CAG'CTG
Cuts at: 2811 3140 2811
Size: 329 6095
SacI G_AGCT'C
Cuts at: 2721 3340 3675 2721
Size: 619 335 5470
Fragments arranged by size:
5470 619 335
SalI G'TCGA_C
Cuts at: 1936 3766 1936
Size: 1830 4594
SbfI CC_TGCA'GG
Cuts at: 3776 3776
Size: 6424
ScaI AGT'ACT
Cuts at: 4672 6384 4672
Size: 1712 4712
SfcI C'TryA_G
Cuts at: 826 1045 3377 3422 3510 3689 3772 4907 5588
Size: 219 2332 45 88 179 83 1135 681
Cuts at: 5588 5779 826
Size: 191 1471
Fragments arranged by size:
2332 1471 1135 681 219 191 179 88
83 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 3469 3469
Size: 6424
SfoI GGC'GCC
Cuts at: 3683 3683
Size: 6424
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
SgrAI Cr'CCGG_yG
Cuts at: 1700 3678 1700
Size: 1978 4446
SmaI CCC'GGG
Cuts at: 1872 3751 1872
Size: 1879 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4528 5429 5691 820
Size: 197 926 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3721 4215 493
Size: 3228 494 2702
Fragments arranged by size:
3228 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6424
SphI G_CATG'C
Cuts at: 1132 3782 1132
Size: 2650 3774
SspI AAT'ATT
Cuts at: 5 52 4348 5
Size: 47 4296 2081
Fragments arranged by size:
4296 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 3607 1517
Size: 654 1436 4334
Fragments arranged by size:
4334 1436 654
StyI C'CwwG_G
Cuts at: 513 1065 3030 3456 3863 513
Size: 552 1965 426 407 3074
Fragments arranged by size:
3074 1965 552 426 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4451 4468 4621 4806 5251 325
Size: 1255 2871 17 153 185 445 1498
Fragments arranged by size:
2871 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 2499 2547
Size: 274 80 33 51 1443 183 339 48
Cuts at: 2547 4670 6382 96
Size: 2123 1712 138
Fragments arranged by size:
2123 1712 1443 339 274 183 138 80
51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2680 2712 3063 3095 6384 6416 2680
Size: 32 351 32 3289 32 2688
Fragments arranged by size:
3289 2688 351 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2134 2735 1316
Size: 818 601 5005
Fragments arranged by size:
5005 818 601
XbaI T'CTAG_A
Cuts at: 3760 3760
Size: 6424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2857 2146
Size: 711 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6424
XmaI C'CCGG_G
Cuts at: 1870 3749 1870
Size: 1879 4545
XmnI GAAnn'nnTTC
Cuts at: 4553 4553
Size: 6424
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4232 276
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SrfI SwaI