Restriction map for
pFN21ASDA1802
(Circular) (Six-base) MAPSORT of: pFN21ASDA1802.seq Check: 4129 from: 1 to: 7165
pFN21ASDA1802 7165 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4975 278
Size: 53 83 186 936 3439 2468
Fragments arranged by size:
3439 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2253 4508 1937
Size: 316 2255 4594
Fragments arranged by size:
4594 2255 316
Acc65I G'GTAC_C
Cuts at: 2123 4486 2123
Size: 2363 4802
AclI AA'CG_TT
Cuts at: 1563 5292 5665 1563
Size: 3729 373 3063
Fragments arranged by size:
3729 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2727 2856 2988 4101 4125 5228 6612 6880
Size: 1095 129 132 1113 24 1103 1384 268
Cuts at: 6880 1632
Size: 1917
Fragments arranged by size:
1917 1384 1113 1103 1095 268 132 129
24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6968
AflIII A'CryG_T
Cuts at: 1203 6064 7041 1203
Size: 4861 977 1327
Fragments arranged by size:
4861 1327 977
AgeI A'CCGG_T
Cuts at: 5996 5996
Size: 7165
AhdI GACnn_n'nnGTC
Cuts at: 5894 5894
Size: 7165
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7165
AlwNI CAG_nnn'CTG
Cuts at: 2336 2597 3159 3908 4908 6480 2336
Size: 261 562 749 1000 1572 3021
Fragments arranged by size:
3021 1572 1000 749 562 261
ApaLI G'TGCA_C
Cuts at: 5222 6378 5222
Size: 1156 6009
ApoI r'AATT_y
Cuts at: 1493 2138 4158 4345 4455 4474 4700 4852 6961
Size: 645 2020 187 110 19 226 152 2109
Cuts at: 6961 1493
Size: 1697
Fragments arranged by size:
2109 2020 1697 645 226 187 152 110
19
AseI AT'TA_AT
Cuts at: 160 1032 5719 160
Size: 872 4687 1606
Fragments arranged by size:
4687 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7165
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 4490 1870
Size: 73 1674 873 4545
Fragments arranged by size:
4545 1674 873 73
AvrII C'CTAG_G
Cuts at: 2921 2981 2921
Size: 60 7105
BamHI G'GATC_C
Cuts at: 4495 4862 4495
Size: 367 6798
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 4486 5941 618
Size: 325 217 627 336 2363 1455 1842
Fragments arranged by size:
2363 1842 1455 627 336 325 217
BanII G_rGCy'C
Cuts at: 1391 3339 3684 3902 4484 1391
Size: 1948 345 218 582 4072
Fragments arranged by size:
4072 1948 582 345 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3459 3490 928
Size: 971 1212 348 31 4603
Fragments arranged by size:
4603 1212 971 348 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5356 5390 1903
Size: 34 3419 34 3678
Fragments arranged by size:
3678 3419 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5057 6267 5057
Size: 1210 5955
BclI T'GATC_A
Cuts at: 1548 3382 3787 3982 1548
Size: 1834 405 195 4731
Fragments arranged by size:
4731 1834 405 195
BfrBI ATG'CAT
Cuts at: 2279 2279
Size: 7165
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5776 136
Size: 107 122 71 631 605 4104 1525
Fragments arranged by size:
4104 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3361 7160 3361
Size: 3799 3366
BlpI GC'TnA_GC
Cuts at: 3909 4582 3909
Size: 673 6492
Bme1580I G_kGCm'C
Cuts at: 1790 3670 5226 6382 1790
Size: 1880 1556 1156 2573
Fragments arranged by size:
2573 1880 1556 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3257 4896 5854
Size: 416 53 83 399 24 1840 1639 958
Cuts at: 5854 442
Size: 1753
Fragments arranged by size:
1840 1753 1639 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 2504 2619 2739 2799 3255 3315 3633
Size: 1194 71 115 120 60 456 60 318
Cuts at: 3633 4463 5825 1239
Size: 830 1362 2579
Fragments arranged by size:
2579 1362 1194 830 456 318 120 115
71 60 60
Bpu10I CC'TnA_GC
Cuts at: 2517 2544 2850 2517
Size: 27 306 6832
Fragments arranged by size:
6832 306 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3863 5290 6155 6453 3863
Size: 1427 865 298 4575
Fragments arranged by size:
4575 1427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2920 3500 5828 882
Size: 617 1421 580 2328 2219
Fragments arranged by size:
2328 2219 1421 617 580
BsaAI yAC'GTr
Cuts at: 493 1204 4462 4956 493
Size: 711 3258 494 2702
Fragments arranged by size:
3258 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7159 7159
Size: 7165
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4972 5354 5989 275
Size: 53 83 186 936 3439 382 635 1451
Fragments arranged by size:
3439 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5598 5996 6270 6417 6828 5598
Size: 398 274 147 411 5935
Fragments arranged by size:
5935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3195 3220 3225 3250 3622
Size: 30 165 30 1792 25 5 25 372
Cuts at: 3622 3652 1178
Size: 30 4691
Fragments arranged by size:
4691 1792 372 165 30 30 30 25
25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2340 2460 4124 4460 1186
Size: 1154 120 1664 336 3891
Fragments arranged by size:
3891 1664 1154 336 120
BseYI C'CCAG_C
Cuts at: 2803 3152 6368 6864 2803
Size: 349 3216 496 3104
Fragments arranged by size:
3216 3104 496 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6592
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4615 5376 6404 1540
Size: 275 186 2614 761 1028 2301
Fragments arranged by size:
2614 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3339 3902 5226 5311 6382 3339
Size: 563 1324 85 1071 4122
Fragments arranged by size:
4122 1324 1071 563 85
BsmI GAATG_Cn'
Cuts at: 2030 4676 4769 2030
Size: 2646 93 4426
Fragments arranged by size:
4426 2646 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3339 3670 3684 3902 4484 5226 5311
Size: 399 1549 331 14 218 582 742 85
Cuts at: 5311 6382 1391
Size: 1071 2174
Fragments arranged by size:
2174 1549 1071 742 582 399 331 218
85 14
BspEI T'CCGG_A
Cuts at: 6828 6828
Size: 7165
BspHI T'CATG_A
Cuts at: 5054 6784 5054
Size: 1730 5435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2413 2566 2659 3046 3613 3982 4520 844
Size: 1569 153 93 387 567 369 538 3489
Fragments arranged by size:
3489 1569 567 538 387 369 153 93
BsrBI CCG'CTC
Cuts at: 1394 1823 5052 1394
Size: 429 3229 3507
Fragments arranged by size:
3507 3229 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2074 5660 5834 65
Size: 1183 826 3586 174 1396
Fragments arranged by size:
3586 1396 1183 826 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4389 5809 5996 1700
Size: 110 2579 1420 187 2869
Fragments arranged by size:
2869 2579 1420 187 110
BsrGI T'GTAC_A
Cuts at: 96 2107 96
Size: 2011 5154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7165
BssSI C'ACGA_G
Cuts at: 5225 6237 5225
Size: 1012 6153
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7165
BstBI TT'CG_AA
Cuts at: 2007 5969 2007
Size: 3962 3203
BstEII G'GTnAC_C
Cuts at: 1184 3354 1184
Size: 2170 4995
BstXI CCAn_nnnn'nTGG
Cuts at: 2292 2790 2955 3366 2292
Size: 498 165 411 6091
Fragments arranged by size:
6091 498 411 165
BstYI r'GATC_y
Cuts at: 1972 2332 3309 3326 3361 4495 4862 5247 5264
Size: 360 977 17 35 1134 367 385 17
Cuts at: 5264 6716 6802 6814 7160 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1134 977 385 367 360 346
86 35 17 17 12
BstZ17I GTA'TAC
Cuts at: 2254 2254
Size: 7165
Bsu36I CC'TnA_GG
Cuts at: 3349 3349
Size: 7165
BtgI C'CryG_G
Cuts at: 513 1065 2022 3439 4300 513
Size: 552 957 1417 861 3378
Fragments arranged by size:
3378 1417 957 861 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3965 487
Size: 683 56 2739 3687
Fragments arranged by size:
3687 2739 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 3985 4681 5475 5495 2209
Size: 1776 696 794 20 3879
Fragments arranged by size:
3879 1776 794 696 20
ClaI AT'CG_AT
Cuts at: 1554 3585 1554
Size: 2031 5134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7130
DraI TTT'AAA
Cuts at: 4469 4469
Size: 7165
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6172 809
Size: 507 4856 1802
Fragments arranged by size:
4856 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4612 5501 7130
Size: 54 1425 50 275 18 2782 889 1629
Cuts at: 7130 8
Size: 43
Fragments arranged by size:
2782 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4612 1537
Size: 275 2800 4090
Fragments arranged by size:
4090 2800 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 2538 4101 4395 5095 1353
Size: 1005 180 1563 294 700 3423
Fragments arranged by size:
3423 1563 1005 700 294 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5752 6126 6272 387
Size: 1064 138 4163 374 146 1280
Fragments arranged by size:
4163 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3337 3900 3337
Size: 563 6602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2504 2619 2727 2739 2799 2856
Size: 393 801 71 115 108 12 60 57
Cuts at: 2856 2988 3255 3315 3633 4101 4125 4463 5228
Size: 132 267 60 318 468 24 338 765
Cuts at: 5228 5825 6612 6880 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 765 597 468 393 338
318 268 267 132 115 108 71 60
60 57 24 12
EcoO109I rG'GnC_Cy
Cuts at: 2976 3017 3521 2976
Size: 41 504 6620
Fragments arranged by size:
6620 504 41
EcoRI G'AATT_C
Cuts at: 4474 4852 4474
Size: 378 6787
EcoRV GAT'ATC
Cuts at: 3562 3562
Size: 7165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3487 3859 3487
Size: 372 6793
FspI TGC'GCA
Cuts at: 5671 5671
Size: 7165
HaeII r_GCGC'y
Cuts at: 1678 6312 1678
Size: 4634 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3301 3333 3989 4021 5806
Size: 32 135 32 1729 32 656 32 1785
Cuts at: 5806 5838 5880 5912 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1785 1729 656 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4509 4755 669
Size: 988 281 2571 246 3079
Fragments arranged by size:
3079 2571 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3371 4092 748
Size: 2623 721 3821
Fragments arranged by size:
3821 2623 721
HpaI GTT'AAC
Cuts at: 4755 4755
Size: 7165
KpnI G_GTAC'C
Cuts at: 2127 4490 2127
Size: 2363 4802
MfeI C'AATT_G
Cuts at: 4764 4764
Size: 7165
MluI A'CGCG_T
Cuts at: 7041 7041
Size: 7165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6279 6463 1305
Size: 4974 184 2007
Fragments arranged by size:
4974 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5343
Fragments arranged by size:
5343 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5123 5482 5641 518
Size: 648 3957 359 159 2042
Fragments arranged by size:
3957 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2807 2939 3388 4581 5258 6406 6651 1606
Size: 1201 132 449 1193 677 1148 245 2120
Fragments arranged by size:
2120 1201 1193 1148 677 449 245 132
NaeI GCC'GGC
Cuts at: 4391 4391
Size: 7165
NcoI C'CATG_G
Cuts at: 513 1065 2022 4300 513
Size: 552 957 2278 3378
Fragments arranged by size:
3378 2278 957 552
NdeI CA'TA_TG
Cuts at: 387 2118 387
Size: 1731 5434
NgoMIV G'CCGG_C
Cuts at: 4389 4389
Size: 7165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7165
NotI GC'GGCC_GC
Cuts at: 4612 4612
Size: 7165
NsiI A_TGCA'T
Cuts at: 2281 2281
Size: 7165
NspI r_CATG'y
Cuts at: 1132 1229 2279 3695 4523 6068 1132
Size: 97 1050 1416 828 1545 2229
Fragments arranged by size:
2229 1545 1416 1050 828 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7165
PciI A'CATG_T
Cuts at: 6064 6064
Size: 7165
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3159 1108
Size: 2051 5114
PfoI T'CCnGG_A
Cuts at: 2034 2721 3055 3088 3128 3158 2034
Size: 687 334 33 40 30 6041
Fragments arranged by size:
6041 687 334 40 33 30
PmeI GTTT'AAAC
Cuts at: 4469 4469
Size: 7165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5234 5266 6747 6779 5234
Size: 32 1481 32 5620
Fragments arranged by size:
5620 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2976 3017 3521 2976
Size: 41 504 6620
Fragments arranged by size:
6620 504 41
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7165
PsiI TTA'TAA
Cuts at: 4735 4735
Size: 7165
PstI C_TGCA'G
Cuts at: 830 4517 830
Size: 3687 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7165
PvuII CAG'CTG
Cuts at: 2807 2807
Size: 7165
SacI G_AGCT'C
Cuts at: 3339 3902 3339
Size: 563 6602
SalI G'TCGA_C
Cuts at: 1936 4507 1936
Size: 2571 4594
SanDI GG'GwC_CC
Cuts at: 2976 2976
Size: 7165
SbfI CC_TGCA'GG
Cuts at: 4517 4517
Size: 7165
ScaI AGT'ACT
Cuts at: 5413 7125 5413
Size: 1712 5453
SexAI A'CCwGG_T
Cuts at: 3517 3517
Size: 7165
SfcI C'TryA_G
Cuts at: 826 1045 2522 4070 4513 5648 6329 6520 826
Size: 219 1477 1548 443 1135 681 191 1471
Fragments arranged by size:
1548 1477 1471 1135 681 443 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7165
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7165
SmaI CCC'GGG
Cuts at: 1872 4492 1872
Size: 2620 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3878 5269 6170 6432 820
Size: 197 926 1935 1391 901 262 1553
Fragments arranged by size:
1935 1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4462 4956 493
Size: 3969 494 2702
Fragments arranged by size:
3969 2702 494
SpeI A'CTAG_T
Cuts at: 152 4043 152
Size: 3891 3274
SphI G_CATG'C
Cuts at: 1132 3695 4523 1132
Size: 2563 828 3774
Fragments arranged by size:
3774 2563 828
SspI AAT'ATT
Cuts at: 5 52 5089 5
Size: 47 5037 2081
Fragments arranged by size:
5037 2081 47
StuI AGG'CCT
Cuts at: 1517 2986 1517
Size: 1469 5696
StyI C'CwwG_G
Cuts at: 513 1065 2022 2789 2855 2888 2921 2981 2987
Size: 552 957 767 66 33 33 60 6
Cuts at: 2987 3020 3062 3095 4300 4604 513
Size: 33 42 33 1205 304 3074
Fragments arranged by size:
3074 1205 957 767 552 304 66 60
42 33 33 33 33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3166 5192 5209 5362 5547 5992 325
Size: 1255 1586 2026 17 153 185 445 1498
Fragments arranged by size:
2026 1586 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2107 2241 3927
Size: 274 80 33 51 1443 130 134 1686
Cuts at: 3927 5411 7123 96
Size: 1484 1712 138
Fragments arranged by size:
1712 1686 1484 1443 274 138 134 130
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 3195 3220 3227 3252 3399 3431 7125
Size: 32 911 25 7 25 147 32 3694
Cuts at: 7125 7157 2252
Size: 32 2260
Fragments arranged by size:
3694 2260 911 147 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7165
XbaI T'CTAG_A
Cuts at: 4058 4501 4058
Size: 443 6722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7165
XmaI C'CCGG_G
Cuts at: 1870 4490 1870
Size: 2620 4545
XmnI GAAnn'nnTTC
Cuts at: 2897 3819 5294 2897
Size: 922 1475 4768
Fragments arranged by size:
4768 1475 922
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4973 276
Size: 53 83 186 936 3439 2468
Fragments arranged by size:
3439 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI BsiWI BsmBI DraIII EcoNI FseI FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI XcmI