Restriction map for
pFN21ASDA1787
(Circular) (Six-base) MAPSORT of: pFN21ASDA1787.seq Check: 2700 from: 1 to: 9415
pFN21ASDA1787 9415 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5536 5615 5875 6016 5536
Size: 79 260 141 8935
Fragments arranged by size:
8935 260 141 79
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7225 278
Size: 53 83 186 936 5689 2468
Fragments arranged by size:
5689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6758 1937
Size: 4821 4594
Acc65I G'GTAC_C
Cuts at: 6736 6736
Size: 9415
AclI AA'CG_TT
Cuts at: 1563 7542 7915 1563
Size: 5979 373 3063
Fragments arranged by size:
5979 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2638 2727 4401 4840 5766 6616 7478
Size: 779 227 89 1674 439 926 850 862
Cuts at: 7478 8862 9130 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1674 1384 926 862 850 779 439
268 227 89
AfeI AGC'GCT
Cuts at: 2346 2346
Size: 9415
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9218
AflIII A'CryG_T
Cuts at: 1203 2275 5223 5736 8314 9291 1203
Size: 1072 2948 513 2578 977 1327
Fragments arranged by size:
2948 2578 1327 1072 977 513
AgeI A'CCGG_T
Cuts at: 8246 8246
Size: 9415
AhdI GACnn_n'nnGTC
Cuts at: 4339 8144 4339
Size: 3805 5610
AleI CACnn'nnGTG
Cuts at: 1166 4070 5480 1166
Size: 2904 1410 5101
Fragments arranged by size:
5101 2904 1410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3716 1184
Size: 2532 6883
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 9383
AlwNI CAG_nnn'CTG
Cuts at: 2470 2519 3575 4100 4751 5207 5539 5915 6344
Size: 49 1056 525 651 456 332 376 429
Cuts at: 6344 7158 8730 2470
Size: 814 1572 3155
Fragments arranged by size:
3155 1572 1056 814 651 525 456 429
376 332 49
ApaI G_GGCC'C
Cuts at: 2096 3998 4288 5727 2096
Size: 1902 290 1439 5784
Fragments arranged by size:
5784 1902 1439 290
ApaLI G'TGCA_C
Cuts at: 2617 4515 6417 7472 8628 2617
Size: 1898 1902 1055 1156 3404
Fragments arranged by size:
3404 1902 1898 1156 1055
ApoI r'AATT_y
Cuts at: 1493 3056 3293 4391 6724 6950 7102 9211 1493
Size: 1563 237 1098 2333 226 152 2109 1697
Fragments arranged by size:
2333 2109 1697 1563 1098 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3372 7969 160
Size: 872 2340 4597 1606
Fragments arranged by size:
4597 2340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9415
AvaI C'yCGr_G
Cuts at: 1870 1943 2687 3530 4018 4594 4976 5417 6183
Size: 73 744 843 488 576 382 441 766
Cuts at: 6183 6426 6586 6740 1870
Size: 243 160 154 4545
Fragments arranged by size:
4545 843 766 744 576 488 441 382
243 160 154 73
BamHI G'GATC_C
Cuts at: 6745 7112 6745
Size: 367 9048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3227 3272 5303 5527 5750
Size: 325 217 627 1440 45 2031 224 223
Cuts at: 5750 6736 8191 618
Size: 986 1455 1842
Fragments arranged by size:
2031 1842 1455 1440 986 627 325 224
223 217 45
BanII G_rGCy'C
Cuts at: 1391 2054 2096 2402 2694 2883 2903 3700 3998
Size: 663 42 306 292 189 20 797 298
Cuts at: 3998 4166 4288 5025 5727 6734 1391
Size: 168 122 737 702 1007 4072
Fragments arranged by size:
4072 1007 797 737 702 663 306 298
292 189 168 122 42 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 2715 4389 4846 5039 5680 928
Size: 971 373 443 1674 457 193 641 4663
Fragments arranged by size:
4663 1674 971 641 457 443 373 193
BbvCI CC'TCA_GC
Cuts at: 4871 4878 4871
Size: 7 9408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4804 4838 6023 6057 6099 6133 6398
Size: 34 2867 34 1185 34 42 34 265
Cuts at: 6398 6432 7606 7640 1903
Size: 34 1174 34 3678
Fragments arranged by size:
3678 2867 1185 1174 265 42 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5059 7307 8517 5059
Size: 2248 1210 5957
Fragments arranged by size:
5957 2248 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3566 5382 5620
Size: 107 122 71 631 605 1894 1816 238
Cuts at: 5620 5748 8026 136
Size: 128 2278 1525
Fragments arranged by size:
2278 1894 1816 1525 631 605 238 128
122 107 71
BglII A'GATC_T
Cuts at: 5706 9410 5706
Size: 3704 5711
BlpI GC'TnA_GC
Cuts at: 3033 3686 6832 3033
Size: 653 3146 5616
Fragments arranged by size:
5616 3146 653
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2621 3275 3903 3998 4017 4288 4511
Size: 306 525 654 628 95 19 271 223
Cuts at: 4511 4519 5532 5650 5727 5744 5841 6421 6691
Size: 8 1013 118 77 17 97 580 270
Cuts at: 6691 7476 8632 1790
Size: 785 1156 2573
Fragments arranged by size:
2573 1156 1013 785 654 628 580 525
306 271 270 223 118 97 95 77
19 17 8
BmgBI CAC'GTC
Cuts at: 1318 4081 1318
Size: 2763 6652
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2175 2349 3407
Size: 416 53 83 399 24 758 174 1058
Cuts at: 3407 3861 4739 5316 7146 8104 442
Size: 454 878 577 1830 958 1753
Fragments arranged by size:
1830 1753 1058 958 878 758 577 454
416 399 174 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2335 3203 3785 4077 4380 5066 5309 5447
Size: 1096 868 582 292 303 686 243 138
Cuts at: 5447 6263 8075 1239
Size: 816 1812 2579
Fragments arranged by size:
2579 1812 1096 868 816 686 582 303
292 243 138
Bpu10I CC'TnA_GC
Cuts at: 2110 2643 2897 3576 4871 4878 5904 6356 6543
Size: 533 254 679 1295 7 1026 452 187
Cuts at: 6543 2110
Size: 4982
Fragments arranged by size:
4982 1295 1026 679 533 452 254 187
7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3902 5687 6668 7540 8405 8703 2822
Size: 1080 1785 981 872 865 298 3534
Fragments arranged by size:
3534 1785 1080 981 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5705 6479 8078 882
Size: 617 4206 774 1599 2219
Fragments arranged by size:
4206 2219 1599 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 5739 6712 7206 493
Size: 711 4535 973 494 2702
Fragments arranged by size:
4535 2702 973 711 494
BsaBI GATnn'nnATC
Cuts at: 3119 5168 9409 3119
Size: 2049 4241 3125
Fragments arranged by size:
4241 3125 2049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3407 4023 4263 7222
Size: 53 83 186 936 1874 616 240 2959
Cuts at: 7222 7604 8239 275
Size: 382 635 1451
Fragments arranged by size:
2959 1874 1451 936 635 616 382 240
186 83 53
BsaWI w'CCGG_w
Cuts at: 5025 7848 8246 8520 8667 9078 5025
Size: 2823 398 274 147 411 5362
Fragments arranged by size:
5362 2823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2885 2915 5296 5326 1178
Size: 30 165 30 1482 30 2381 30 5267
Fragments arranged by size:
5267 2381 1482 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2468 4817 5306 5363 5381 6162 6413 6414
Size: 1282 2349 489 57 18 781 251 1
Cuts at: 6414 6446 6671 1186
Size: 32 225 3930
Fragments arranged by size:
3930 2349 1282 781 489 251 225 57
32 18 1
BseYI C'CCAG_C
Cuts at: 2096 2519 2764 3199 4504 4939 5116 5307 5532
Size: 423 245 435 1305 435 177 191 225
Cuts at: 5532 5841 5994 6248 6331 6476 6525 8618 9114
Size: 309 153 254 83 145 49 2093 496
Cuts at: 9114 2096
Size: 2397
Fragments arranged by size:
2397 2093 1305 496 435 435 423 309
254 245 225 191 177 153 145 83
49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 4172 4538 4721 5564 6232 1163
Size: 573 402 2034 366 183 843 668 4346
Fragments arranged by size:
4346 2034 843 668 573 402 366 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2299 2780 6865 7626 8654 1540
Size: 275 186 298 481 4085 761 1028 2301
Fragments arranged by size:
4085 2301 1028 761 481 298 275 186
BsiHKAI G_wGCw'C
Cuts at: 2621 2903 3541 4519 5025 6421 7476 7561 8632
Size: 282 638 978 506 1396 1055 85 1071
Cuts at: 8632 2621
Size: 3404
Fragments arranged by size:
3404 1396 1071 1055 978 638 506 282
85
BsiWI C'GTAC_G
Cuts at: 2198 5468 2198
Size: 3270 6145
BsmI GAATG_Cn'
Cuts at: 6926 7019 6926
Size: 93 9322
BsmBI CGTCTCn'nnnn_
Cuts at: 2777 3497 6144 2777
Size: 720 2647 6048
Fragments arranged by size:
6048 2647 720
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2096 2402 2621 2694 2883 2903
Size: 399 264 42 306 219 73 189 20
Cuts at: 2903 3275 3541 3700 3903 3998 4017 4166 4288
Size: 372 266 159 203 95 19 149 122
Cuts at: 4288 4511 4519 5025 5532 5650 5727 5744 5841
Size: 223 8 506 507 118 77 17 97
Cuts at: 5841 6421 6691 6734 7476 7561 8632 1391
Size: 580 270 43 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 580 507 506 399 372
306 270 266 264 223 219 203 189
159 149 122 118 97 95 85 77
73 43 42 20 19 17 8
BspEI T'CCGG_A
Cuts at: 5025 9078 5025
Size: 4053 5362
BspHI T'CATG_A
Cuts at: 2983 3075 3234 7304 9034 2983
Size: 92 159 4070 1730 3364
Fragments arranged by size:
4070 3364 1730 159 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4179 4560 4916 5536 5615 5875 6016 6204
Size: 3335 381 356 620 79 260 141 188
Cuts at: 6204 6329 6770 844
Size: 125 441 3489
Fragments arranged by size:
3489 3335 620 441 381 356 260 188
141 125 79
BsrBI CCG'CTC
Cuts at: 1394 1823 2106 2319 3243 7302 1394
Size: 429 283 213 924 4059 3507
Fragments arranged by size:
4059 3507 924 429 283 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 5211 6298 7910 8084 65
Size: 1183 3963 1087 1612 174 1396
Fragments arranged by size:
3963 1612 1396 1183 1087 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2908 3039 4676 5333 8059 8246 1700
Size: 110 1098 131 1637 657 2726 187 2869
Fragments arranged by size:
2869 2726 1637 1098 657 187 131 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9415
BssHII G'CGCG_C
Cuts at: 1925 5610 1925
Size: 3685 5730
BssSI C'ACGA_G
Cuts at: 2401 2583 3626 4813 5281 7475 8487 2401
Size: 182 1043 1187 468 2194 1012 3329
Fragments arranged by size:
3329 2194 1187 1043 1012 468 182
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4826 6344 794
Size: 4032 1518 3865
Fragments arranged by size:
4032 3865 1518
BstBI TT'CG_AA
Cuts at: 2007 8219 2007
Size: 6212 3203
BstEII G'GTnAC_C
Cuts at: 1184 3166 3320 3457 1184
Size: 1982 154 137 7142
Fragments arranged by size:
7142 1982 154 137
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 3190 4072 5044 5287 5482 2362
Size: 828 882 972 243 195 6295
Fragments arranged by size:
6295 972 882 828 243 195
BstYI r'GATC_y
Cuts at: 1972 2927 3114 5239 5706 6745 7112 7497 7514
Size: 955 187 2125 467 1039 367 385 17
Cuts at: 7514 8966 9052 9064 9410 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2125 1977 1452 1039 955 467 385 367
346 187 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3644 4113 4150 3644
Size: 469 37 8909
Fragments arranged by size:
8909 469 37
BtgI C'CryG_G
Cuts at: 513 1065 2022 2236 2382 4683 5257 5434 5680
Size: 552 957 214 146 2301 574 177 246
Cuts at: 5680 513
Size: 4248
Fragments arranged by size:
4248 2301 957 574 552 246 214 177
146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2283 487
Size: 683 56 1057 7619
Fragments arranged by size:
7619 1057 683 56
BtsI GCAGTG_nn'
Cuts at: 2383 3456 3636 4710 4733 4827 5344 5882 6194
Size: 1073 180 1074 23 94 517 538 312
Cuts at: 6194 6931 7725 7745 2383
Size: 737 794 20 4053
Fragments arranged by size:
4053 1074 1073 794 737 538 517 312
180 94 23 20
ClaI AT'CG_AT
Cuts at: 1554 3381 1554
Size: 1827 7588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4760 4795 5194 5229 529
Size: 35 4196 35 399 35 4715
Fragments arranged by size:
4715 4196 399 35 35 35
DraI TTT'AAA
Cuts at: 6719 6719
Size: 9415
DraIII CAC_nnn'GTG
Cuts at: 3679 3679
Size: 9415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2303 4268 8422 809
Size: 507 987 1965 4154 1802
Fragments arranged by size:
4154 1965 1802 987 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2777 4048 4570
Size: 54 1425 50 275 18 947 1271 522
Cuts at: 4570 4642 5098 5472 5484 6862 7751 9380 8
Size: 72 456 374 12 1378 889 1629 43
Fragments arranged by size:
1629 1425 1378 1271 947 889 522 456
374 275 72 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2777 6862 1537
Size: 275 965 4085 4090
Fragments arranged by size:
4090 4085 965 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3593 4899 7345 1353
Size: 2240 1306 2446 3423
Fragments arranged by size:
3423 2446 2240 1306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3485 3533 4223 6090 6497 8002
Size: 1064 138 1896 48 690 1867 407 1505
Cuts at: 8002 8376 8522 387
Size: 374 146 1280
Fragments arranged by size:
1896 1867 1505 1280 1064 690 407 374
146 138 48
EcoICRI GAG'CTC
Cuts at: 2901 5023 2901
Size: 2122 7293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2335 2411 2638 2727 3203 3785 4077
Size: 393 703 76 227 89 476 582 292
Cuts at: 4077 4380 4401 4840 5066 5309 5447 5766 6263
Size: 303 21 439 226 243 138 319 497
Cuts at: 6263 6616 7478 8075 8862 9130 1239
Size: 353 862 597 787 268 1524
Fragments arranged by size:
1524 862 787 703 597 582 497 476
439 393 353 319 303 292 268 243
227 226 138 89 76 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2646 5907 6387 2646
Size: 3261 480 5674
Fragments arranged by size:
5674 3261 480
EcoO109I rG'GnC_Cy
Cuts at: 2063 2092 2093 2467 2733 2997 3648 3837 3994
Size: 29 1 374 266 264 651 189 157
Cuts at: 3994 3995 4285 4973 5195 6514 6590 2063
Size: 1 290 688 222 1319 76 4888
Fragments arranged by size:
4888 1319 688 651 374 290 266 264
222 189 157 76 29 1 1
EcoRI G'AATT_C
Cuts at: 3056 3293 4391 6724 7102 3056
Size: 237 1098 2333 378 5369
Fragments arranged by size:
5369 2333 1098 378 237
FspI TGC'GCA
Cuts at: 3728 4475 7921 3728
Size: 747 3446 5222
Fragments arranged by size:
5222 3446 747
FspAI rTGC'GCAy
Cuts at: 3728 3728
Size: 9415
HaeII r_GCGC'y
Cuts at: 1678 2173 2348 3040 3524 3583 4587 8562 1678
Size: 495 175 692 484 59 1004 3975 2531
Fragments arranged by size:
3975 2531 1004 692 495 484 175 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 3128 3160 4325
Size: 32 135 32 854 32 670 32 1165
Cuts at: 4325 4357 6349 6381 6506 6538 8056 8088 8130
Size: 32 1992 32 125 32 1518 32 42
Cuts at: 8130 8162 1373
Size: 32 2626
Fragments arranged by size:
2626 1992 1518 1165 854 670 135 125
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 6759 7005 669
Size: 988 281 370 4451 246 3079
Fragments arranged by size:
4451 3079 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 3128 748
Size: 2380 7035
HpaI GTT'AAC
Cuts at: 7005 7005
Size: 9415
KpnI G_GTAC'C
Cuts at: 6740 6740
Size: 9415
MfeI C'AATT_G
Cuts at: 5268 7014 5268
Size: 1746 7669
MluI A'CGCG_T
Cuts at: 2275 9291 2275
Size: 7016 2399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2148 2430 2988 3372 4302 5217 8529 8713
Size: 843 282 558 384 930 915 3312 184
Cuts at: 8713 1305
Size: 2007
Fragments arranged by size:
3312 2007 930 915 843 558 384 282
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4050 4644 5486 10
Size: 54 1425 343 2218 594 842 3939
Fragments arranged by size:
3939 2218 1425 842 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3233 3236 4070 4430 4526 5042 5174
Size: 648 2067 3 834 360 96 516 132
Cuts at: 5174 5480 5796 5802 7373 7732 7891 518
Size: 306 316 6 1571 359 159 2042
Fragments arranged by size:
2067 2042 1571 834 648 516 360 359
316 306 159 132 96 6 3
MspA1I CmG'CkG
Cuts at: 1606 2100 2142 4186 4338 4832 5055 5259 5536
Size: 494 42 2044 152 494 223 204 277
Cuts at: 5536 6302 6529 6602 6831 7508 8656 8901 1606
Size: 766 227 73 229 677 1148 245 2120
Fragments arranged by size:
2120 2044 1148 766 677 494 494 277
245 229 227 223 204 152 73 42
NaeI GCC'GGC
Cuts at: 3041 3041
Size: 9415
NcoI C'CATG_G
Cuts at: 513 1065 2022 4683 5434 513
Size: 552 957 2661 751 4494
Fragments arranged by size:
4494 2661 957 751 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9415
NgoMIV G'CCGG_C
Cuts at: 3039 3039
Size: 9415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9415
NotI GC'GGCC_GC
Cuts at: 6862 6862
Size: 9415
NspI r_CATG'y
Cuts at: 1132 1229 6773 8318 1132
Size: 97 5544 1545 2229
Fragments arranged by size:
5544 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4997 1348
Size: 3649 5766
PciI A'CATG_T
Cuts at: 8314 8314
Size: 9415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 4751 5524 5539 6267 1108
Size: 1134 2509 773 15 728 4256
Fragments arranged by size:
4256 2509 1134 773 728 15
PfoI T'CCnGG_A
Cuts at: 2312 3885 5424 6510 2312
Size: 1573 1539 1086 5217
Fragments arranged by size:
5217 1573 1539 1086
PmeI GTTT'AAAC
Cuts at: 6719 6719
Size: 9415
PmlI CAC'GTG
Cuts at: 5739 5739
Size: 9415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 7484 7516 8997 9029 2885
Size: 32 4567 32 1481 32 3271
Fragments arranged by size:
4567 3271 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2733 2997 3837 4973 6514 6590 2467
Size: 266 264 840 1136 1541 76 5292
Fragments arranged by size:
5292 1541 1136 840 266 264 76
PshAI GACnn'nnGTC
Cuts at: 1613 2119 3464 1613
Size: 506 1345 7564
Fragments arranged by size:
7564 1345 506
PsiI TTA'TAA
Cuts at: 6985 6985
Size: 9415
PspOMI G'GGCC_C
Cuts at: 2092 3994 4284 5723 2092
Size: 1902 290 1439 5784
Fragments arranged by size:
5784 1902 1439 290
PspXI vC'TCGA_Gb
Cuts at: 2687 5417 2687
Size: 2730 6685
PstI C_TGCA'G
Cuts at: 830 2743 5203 6767 830
Size: 1913 2460 1564 3478
Fragments arranged by size:
3478 2460 1913 1564
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9415
PvuII CAG'CTG
Cuts at: 4186 5055 5536 4186
Size: 869 481 8065
Fragments arranged by size:
8065 869 481
SacI G_AGCT'C
Cuts at: 2903 5025 2903
Size: 2122 7293
SacII CC_GC'GG
Cuts at: 5260 5260
Size: 9415
SalI G'TCGA_C
Cuts at: 1936 6757 1936
Size: 4821 4594
SanDI GG'GwC_CC
Cuts at: 2997 4973 6514 6590 2997
Size: 1976 1541 76 5822
Fragments arranged by size:
5822 1976 1541 76
SapI GCTCTTCn'nnn_
Cuts at: 3593 4899 3593
Size: 1306 8109
SbfI CC_TGCA'GG
Cuts at: 6767 6767
Size: 9415
ScaI AGT'ACT
Cuts at: 3287 4777 7663 9375 3287
Size: 1490 2886 1712 3327
Fragments arranged by size:
3327 2886 1712 1490
SexAI A'CCwGG_T
Cuts at: 4125 4125
Size: 9415
SfcI C'TryA_G
Cuts at: 826 1045 2739 5199 6763 7898 8579 8770 826
Size: 219 1694 2460 1564 1135 681 191 1471
Fragments arranged by size:
2460 1694 1564 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5382 5382
Size: 9415
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9415
SgrAI Cr'CCGG_yG
Cuts at: 1700 3039 1700
Size: 1339 8076
SmaI CCC'GGG
Cuts at: 1872 4020 4978 6185 6588 6742 1872
Size: 2148 958 1207 403 154 4545
Fragments arranged by size:
4545 2148 1207 958 403 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 2801 3917 5417 5702 6647
Size: 197 926 744 114 1116 1500 285 945
Cuts at: 6647 7519 8420 8682 820
Size: 872 901 262 1553
Fragments arranged by size:
1553 1500 1116 945 926 901 872 744
285 262 197 114
SnaBI TAC'GTA
Cuts at: 493 6712 7206 493
Size: 6219 494 2702
Fragments arranged by size:
6219 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9415
SphI G_CATG'C
Cuts at: 1132 6773 1132
Size: 5641 3774
SspI AAT'ATT
Cuts at: 5 52 7339 5
Size: 47 7287 2081
Fragments arranged by size:
7287 2081 47
StuI AGG'CCT
Cuts at: 1517 2574 2922 1517
Size: 1057 348 8010
Fragments arranged by size:
8010 1057 348
StyI C'CwwG_G
Cuts at: 513 1065 2022 2694 2718 4542 4579 4683 4863
Size: 552 957 672 24 1824 37 104 180
Cuts at: 4863 5434 5941 6607 6854 513
Size: 571 507 666 247 3074
Fragments arranged by size:
3074 1824 957 672 666 571 552 507
247 180 104 37 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2527 2636 3188 3638 3797 4476 5126
Size: 1255 947 109 552 450 159 679 650
Cuts at: 5126 5321 6336 7442 7459 7612 7797 8242 325
Size: 195 1015 1106 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1106 1015 947 679 650 552
450 445 195 185 159 153 109 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 4775 7661
Size: 274 80 33 51 1443 1308 1490 2886
Cuts at: 7661 9373 96
Size: 1712 138
Fragments arranged by size:
2886 1712 1490 1443 1308 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 4423 4455 5296 5328 9375 9407 2430
Size: 32 1961 32 841 32 4047 32 2438
Fragments arranged by size:
4047 2438 1961 841 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9415
XbaI T'CTAG_A
Cuts at: 4798 6751 4798
Size: 1953 7462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 3599 4681 2131
Size: 1468 1082 6865
Fragments arranged by size:
6865 1468 1082
XhoI C'TCGA_G
Cuts at: 1943 2687 5417 1943
Size: 744 2730 5941
Fragments arranged by size:
5941 2730 744
XmaI C'CCGG_G
Cuts at: 1870 4018 4976 6183 6586 6740 1870
Size: 2148 958 1207 403 154 4545
Fragments arranged by size:
4545 2148 1207 958 403 154
XmnI GAAnn'nnTTC
Cuts at: 4727 7544 4727
Size: 2817 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7223 276
Size: 53 83 186 936 5689 2468
Fragments arranged by size:
5689 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BstZ17I EcoRV
FalI FseI KasI NarI NruI NsiI PacI PsrI
RsrII SfoI SrfI SwaI