Restriction map for
pFN21ASDA1785
(Circular) (Six-base) MAPSORT of: pFN21ASDA1785.seq Check: 915 from: 1 to: 6580
pFN21ASDA1785 6580 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4390 278
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2790 3923 1937
Size: 853 1133 4594
Fragments arranged by size:
4594 1133 853
Acc65I G'GTAC_C
Cuts at: 3519 3901 3519
Size: 382 6198
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 3454 4643 6027 6295 1632
Size: 698 1124 1189 1384 268 1917
Fragments arranged by size:
1917 1384 1189 1124 698 268
AflII C'TTAA_G
Cuts at: 820 1017 3440 820
Size: 197 2423 3960
Fragments arranged by size:
3960 2423 197
AflIII A'CryG_T
Cuts at: 1203 3506 5479 6456 1203
Size: 2303 1973 977 1327
Fragments arranged by size:
2303 1973 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2232 2604 3642 3804 1184
Size: 1048 372 1038 162 3960
Fragments arranged by size:
3960 1048 1038 372 162
AlwNI CAG_nnn'CTG
Cuts at: 2597 3074 4323 5895 2597
Size: 477 1249 1572 3282
Fragments arranged by size:
3282 1572 1249 477
ApaLI G'TGCA_C
Cuts at: 3675 4637 5793 3675
Size: 962 1156 4462
Fragments arranged by size:
4462 1156 962
ApoI r'AATT_y
Cuts at: 1493 2195 3079 3533 3748 3889 4115 4267 6376
Size: 702 884 454 215 141 226 152 2109
Cuts at: 6376 1493
Size: 1697
Fragments arranged by size:
2109 1697 884 702 454 226 215 152
141
AseI AT'TA_AT
Cuts at: 160 1032 2757 2993 5134 160
Size: 872 1725 236 2141 1606
Fragments arranged by size:
2141 1725 1606 872 236
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 3905 1870
Size: 73 112 1850 4545
Fragments arranged by size:
4545 1850 112 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3222 3255 3222
Size: 33 6547
BamHI G'GATC_C
Cuts at: 3139 3910 4277 3139
Size: 771 367 5442
Fragments arranged by size:
5442 771 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2178 3519 3901 5356 618
Size: 325 217 627 391 1341 382 1455 1842
Fragments arranged by size:
1842 1455 1341 627 391 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2240 2275 3899 1391
Size: 849 35 1624 4072
Fragments arranged by size:
4072 1624 849 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4771 4805 1903
Size: 34 2834 34 3678
Fragments arranged by size:
3678 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6580
BfrBI ATG'CAT
Cuts at: 3093 3093
Size: 6580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2803 6575 2803
Size: 3772 2808
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 2181 3679 4641 5797 1790
Size: 391 1498 962 1156 2573
Fragments arranged by size:
2573 1498 1156 962 391
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4311 5269 442
Size: 416 53 83 399 24 2894 958 1753
Fragments arranged by size:
2894 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5240 1239
Size: 4001 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4705 5570 5868 4705
Size: 865 298 5417
Fragments arranged by size:
5417 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 882
Size: 617 3744 2219
Fragments arranged by size:
3744 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2475 3877 4371 493
Size: 711 1271 1402 494 2702
Fragments arranged by size:
2702 1402 1271 711 494
BsaBI GATnn'nnATC
Cuts at: 6574 6574
Size: 6580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4387 4769 5404 275
Size: 53 83 186 936 2854 382 635 1451
Fragments arranged by size:
2854 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5013 5411 5685 5832 6243 5013
Size: 398 274 147 411 5350
Fragments arranged by size:
5350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2331 2361 1178
Size: 30 165 30 928 30 5397
Fragments arranged by size:
5397 928 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2367 1186
Size: 937 244 5399
Fragments arranged by size:
5399 937 244
BseYI C'CCAG_C
Cuts at: 3226 5783 6279 3226
Size: 2557 496 3527
Fragments arranged by size:
3527 2557 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2610 2955 1163
Size: 573 874 345 4788
Fragments arranged by size:
4788 874 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3679 4641 4726 5797 3679
Size: 962 85 1071 4462
Fragments arranged by size:
4462 1071 962 85
BsmI GAATG_Cn'
Cuts at: 2985 4091 4184 2985
Size: 1106 93 5381
Fragments arranged by size:
5381 1106 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2240 2275 3679 3899 4641 4726
Size: 399 391 59 35 1404 220 742 85
Cuts at: 4726 5797 1391
Size: 1071 2174
Fragments arranged by size:
2174 1404 1071 742 399 391 220 85
59 35
BspEI T'CCGG_A
Cuts at: 6243 6243
Size: 6580
BspHI T'CATG_A
Cuts at: 2613 3651 4469 6199 2613
Size: 1038 818 1730 2994
Fragments arranged by size:
2994 1730 1038 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3935 844
Size: 3091 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4467 1394
Size: 429 2644 3507
Fragments arranged by size:
3507 2644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2216 2576 2844 3776 5075 5249 65
Size: 1183 968 360 268 932 1299 174 1396
Fragments arranged by size:
1396 1299 1183 968 932 360 268 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5224 5411 1700
Size: 110 3414 187 2869
Fragments arranged by size:
3414 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 3795 4640 5652 3795
Size: 845 1012 4723
Fragments arranged by size:
4723 1012 845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3267 794
Size: 2473 4107
BstBI TT'CG_AA
Cuts at: 2007 5384 2007
Size: 3377 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2089
Size: 6580
BstYI r'GATC_y
Cuts at: 1972 2026 2803 3139 3910 4277 4662 4679 6131
Size: 54 777 336 771 367 385 17 1452
Cuts at: 6131 6217 6229 6575 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 777 771 385 367 346 336
86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5071
Fragments arranged by size:
5071 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3788 4096 4890 4910 2221
Size: 1567 308 794 20 3891
Fragments arranged by size:
3891 1567 794 308 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2691 2726 529
Size: 35 2127 35 4383
Fragments arranged by size:
4383 2127 35 35
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2401 4027
Size: 54 1425 50 275 18 333 238 1626
Cuts at: 4027 4916 6545 8
Size: 889 1629 43
Fragments arranged by size:
1629 1626 1425 889 333 275 238 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 2934 3483 4510 1353
Size: 1032 549 549 1027 3423
Fragments arranged by size:
3423 1032 1027 549 549
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 3454 4643 5240 6027 6295 1239
Size: 393 698 1124 1189 597 787 268 1524
Fragments arranged by size:
1524 1189 1124 787 698 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3343 3343
Size: 6580
EcoRI G'AATT_C
Cuts at: 2195 3889 4267 2195
Size: 1694 378 4508
Fragments arranged by size:
4508 1694 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3484 3484
Size: 6580
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 3060 5727 1678
Size: 1382 2667 2531
Fragments arranged by size:
2667 2531 1382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2331 2363 3045 3077 5221
Size: 32 135 32 759 32 682 32 2144
Cuts at: 5221 5253 5295 5327 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2144 759 682 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 3924 4170 669
Size: 988 281 412 1574 246 3079
Fragments arranged by size:
3079 1574 988 412 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6580
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3523 3905 3523
Size: 382 6198
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5694 5878 1305
Size: 4389 184 2007
Fragments arranged by size:
4389 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2403 10
Size: 54 1425 343 571 4187
Fragments arranged by size:
4187 1425 571 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2586 2618 3684 3781 4538 4897 5056
Size: 648 1420 32 1066 97 757 359 159
Cuts at: 5056 518
Size: 2042
Fragments arranged by size:
2042 1420 1066 757 648 359 159 97
32
MspA1I CmG'CkG
Cuts at: 1606 3320 3996 4673 5821 6066 1606
Size: 1714 676 677 1148 245 2120
Fragments arranged by size:
2120 1714 1148 677 676 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5071
Fragments arranged by size:
5071 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NsiI A_TGCA'T
Cuts at: 3095 3095
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 3510 3938 5483 1132
Size: 97 2281 428 1545 2229
Fragments arranged by size:
2281 2229 1545 428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 3506 5479 3506
Size: 1973 4607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6580
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3609 1613
Size: 1996 4584
PsiI TTA'TAA
Cuts at: 3825 4150 3825
Size: 325 6255
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 6548
PstI C_TGCA'G
Cuts at: 830 2299 3932 830
Size: 1469 1633 3478
Fragments arranged by size:
3478 1633 1469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 3320 3320
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SapI GCTCTTCn'nnn_
Cuts at: 2934 2934
Size: 6580
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SfcI C'TryA_G
Cuts at: 826 1045 2295 3203 3928 5063 5744 5935 826
Size: 219 1250 908 725 1135 681 191 1471
Fragments arranged by size:
1471 1250 1135 908 725 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3440 4684 5585 5847 820
Size: 197 926 1497 1244 901 262 1553
Fragments arranged by size:
1553 1497 1244 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3877 4371 493
Size: 3384 494 2702
Fragments arranged by size:
3384 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 2707 2869 4504 5
Size: 47 2655 162 1635 2081
Fragments arranged by size:
2655 2081 1635 162 47
StuI AGG'CCT
Cuts at: 1517 3222 1517
Size: 1705 4875
StyI C'CwwG_G
Cuts at: 513 1065 2022 2919 3452 4019 513
Size: 552 957 897 533 567 3074
Fragments arranged by size:
3074 957 897 567 552 533
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4607 4624 4777 4962 5407 325
Size: 1255 3027 17 153 185 445 1498
Fragments arranged by size:
3027 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3392 4826 6538
Size: 274 80 33 51 1443 1415 1434 1712
Cuts at: 6538 96
Size: 138
Fragments arranged by size:
1712 1443 1434 1415 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6540 6572 6540
Size: 32 6548
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 2764 3397 3916 2764
Size: 633 519 5428
Fragments arranged by size:
5428 633 519
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 3006 3467 4709 3006
Size: 461 1242 4877
Fragments arranged by size:
4877 1242 461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4388 276
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI