Restriction map for
pFN21ASDA1783
(Circular) (Six-base) MAPSORT of: pFN21ASDA1783.seq Check: 4065 from: 1 to: 14017
pFN21ASDA1783 14017 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2941 6260 10431 10858 11148 2941
Size: 3319 4171 427 290 5810
Fragments arranged by size:
5810 4171 3319 427 290
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11827 278
Size: 53 83 186 936 10291 2468
Fragments arranged by size:
10291 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6228 10238 11360 1937
Size: 4291 4010 1122 4594
Fragments arranged by size:
4594 4291 4010 1122
Acc65I G'GTAC_C
Cuts at: 3212 5083 6321 11338 3212
Size: 1871 1238 5017 5891
Fragments arranged by size:
5891 5017 1871 1238
AclI AA'CG_TT
Cuts at: 1563 12144 12517 1563
Size: 10581 373 3063
Fragments arranged by size:
10581 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3729 4928 6533 7147 8091 8956 9323 9401
Size: 2097 1199 1605 614 944 865 367 78
Cuts at: 9401 10121 10712 11138 12080 13464 13732 1632
Size: 720 591 426 942 1384 268 1917
Fragments arranged by size:
2097 1917 1605 1384 1199 944 942 865
720 614 591 426 367 268 78
AfeI AGC'GCT
Cuts at: 2625 6212 7026 2625
Size: 3587 814 9616
Fragments arranged by size:
9616 3587 814
AflII C'TTAA_G
Cuts at: 820 1017 9458 820
Size: 197 8441 5379
Fragments arranged by size:
8441 5379 197
AflIII A'CryG_T
Cuts at: 1203 2469 5938 12916 13893 1203
Size: 1266 3469 6978 977 1327
Fragments arranged by size:
6978 3469 1327 1266 977
AgeI A'CCGG_T
Cuts at: 4311 12848 4311
Size: 8537 5480
AhdI GACnn_n'nnGTC
Cuts at: 12746 12746
Size: 14017
AleI CACnn'nnGTG
Cuts at: 1166 4470 5092 5297 5589 1166
Size: 3304 622 205 292 9594
Fragments arranged by size:
9594 3304 622 292 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7045 9239 9657 1184
Size: 5861 2194 418 5544
Fragments arranged by size:
5861 5544 2194 418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10865 10897 10865
Size: 32 13985
AlwNI CAG_nnn'CTG
Cuts at: 2056 2267 2496 2658 5398 5660 5839 6049 6115
Size: 211 229 162 2740 262 179 210 66
Cuts at: 6115 6187 6223 6382 6403 6688 7751 8749 10714
Size: 72 36 159 21 285 1063 998 1965
Cuts at: 10714 10834 10909 11029 11098 11245 11760 13332 2056
Size: 120 75 120 69 147 515 1572 2741
Fragments arranged by size:
2741 2740 1965 1572 1063 998 515 285
262 229 211 210 179 162 159 147
120 120 75 72 69 66 36 21
ApaI G_GGCC'C
Cuts at: 2328 2424 2709 3095 6928 7093 7641 8501 8645
Size: 96 285 386 3833 165 548 860 144
Cuts at: 8645 8694 8724 11169 2328
Size: 49 30 2445 5176
Fragments arranged by size:
5176 3833 2445 860 548 386 285 165
144 96 49 30
ApaLI G'TGCA_C
Cuts at: 12074 13230 12074
Size: 1156 12861
ApoI r'AATT_y
Cuts at: 1493 9857 10535 11326 11552 11704 13813 1493
Size: 8364 678 791 226 152 2109 1697
Fragments arranged by size:
8364 2109 1697 791 678 226 152
AscI GG'CGCG_CC
Cuts at: 2118 2118
Size: 14017
AseI AT'TA_AT
Cuts at: 160 1032 12571 160
Size: 872 11539 1606
Fragments arranged by size:
11539 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14017
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2553 2676 2746 2781 2829 2913
Size: 73 123 487 123 70 35 48 84
Cuts at: 2913 3494 4030 7075 7086 7260 7932 8494 8801
Size: 581 536 3045 11 174 672 562 307
Cuts at: 8801 9159 9202 9411 10062 10191 11342 1870
Size: 358 43 209 651 129 1151 4545
Fragments arranged by size:
4545 3045 1151 672 651 581 562 536
487 358 307 209 174 129 123 123
84 73 70 48 43 35 11
AvrII C'CTAG_G
Cuts at: 3962 3962
Size: 14017
BamHI G'GATC_C
Cuts at: 3669 6776 7227 11347 11714 3669
Size: 3107 451 4120 367 5972
Fragments arranged by size:
5972 4120 3107 451 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2346 2879 2953 3071 3212
Size: 325 217 627 559 533 74 118 141
Cuts at: 3212 3504 3971 4132 4148 5083 5257 5285 5446
Size: 292 467 161 16 935 174 28 161
Cuts at: 5446 5984 6095 6321 7252 7628 7771 8597 9914
Size: 538 111 226 931 376 143 826 1317
Cuts at: 9914 10347 11338 12793 618
Size: 433 991 1455 1842
Fragments arranged by size:
1842 1455 1317 991 935 931 826 627
559 538 533 467 433 376 325 292
226 217 174 161 161 143 141 118
111 74 28 16
BanII G_rGCy'C
Cuts at: 1391 2328 2424 2594 2709 2780 2827 2920 3095
Size: 937 96 170 115 71 47 93 175
Cuts at: 3095 3142 3495 3921 3944 4926 5784 6198 6861
Size: 47 353 426 23 982 858 414 663
Cuts at: 6861 6928 7093 7603 7641 8501 8645 8694 8724
Size: 67 165 510 38 860 144 49 30
Cuts at: 8724 11055 11169 11336 1391
Size: 2331 114 167 4072
Fragments arranged by size:
4072 2331 982 937 860 858 663 510
426 414 353 175 170 167 165 144
115 114 96 93 71 67 49 47
47 38 30 23
BbeI G_GCGC'C
Cuts at: 2350 2883 3075 3975 4136 7256 7632 10351 2350
Size: 533 192 900 161 3120 376 2719 6016
Fragments arranged by size:
6016 3120 2719 900 533 376 192 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4188 4499 4645 6075 6521 8079 9132
Size: 971 2289 311 146 1430 446 1558 1053
Cuts at: 9132 11254 928
Size: 2122 3691
Fragments arranged by size:
3691 2289 2122 1558 1430 1053 971 446
311 146
BbvCI CC'TCA_GC
Cuts at: 4370 5457 6726 7577 7966 8340 9521 9980 10124
Size: 1087 1269 851 389 374 1181 459 144
Cuts at: 10124 10208 10796 4370
Size: 84 588 7591
Fragments arranged by size:
7591 1269 1181 1087 851 588 459 389
374 144 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3034 3068 4147 4181 5341 5375 7913
Size: 34 1097 34 1079 34 1160 34 2538
Cuts at: 7913 7947 10390 10424 12208 12242 1903
Size: 34 2443 34 1784 34 3678
Fragments arranged by size:
3678 2538 2443 1784 1160 1097 1079 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5528 9050 11909 13119 5528
Size: 3522 2859 1210 6426
Fragments arranged by size:
6426 3522 2859 1210
BclI T'GATC_A
Cuts at: 1548 3619 7335 1548
Size: 2071 3716 8230
Fragments arranged by size:
8230 3716 2071
BfrBI ATG'CAT
Cuts at: 4446 4446
Size: 14017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2071 2544 2909
Size: 107 122 71 631 605 399 473 365
Cuts at: 2909 3023 3105 3528 4400 4644 4958 6032 6918
Size: 114 82 423 872 244 314 1074 886
Cuts at: 6918 6932 7079 7088 7533 7943 8440 8496 8527
Size: 14 147 9 445 410 497 56 31
Cuts at: 8527 9357 9428 9580 10778 12628 136
Size: 830 71 152 1198 1850 1525
Fragments arranged by size:
1850 1525 1198 1074 886 872 830 631
605 497 473 445 423 410 399 365
314 244 152 147 122 114 107 82
71 71 56 31 14 9
BglII A'GATC_T
Cuts at: 7548 14012 7548
Size: 6464 7553
BlpI GC'TnA_GC
Cuts at: 3532 4109 4746 6495 6944 7402 8346 11144 11194
Size: 577 637 1749 449 458 944 2798 50
Cuts at: 11194 11434 3532
Size: 240 6115
Fragments arranged by size:
6115 2798 1749 944 637 577 458 449
240 50
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2424 2709 3095 3777 4479 4937 5987
Size: 538 96 285 386 682 702 458 1050
Cuts at: 5987 6018 6736 6928 7093 7641 7745 8501 8514
Size: 31 718 192 165 548 104 756 13
Cuts at: 8514 8602 8645 8694 8724 11169 12078 13234 1790
Size: 88 43 49 30 2445 909 1156 2573
Fragments arranged by size:
2573 2445 1156 1050 909 756 718 702
682 548 538 458 386 285 192 165
104 96 88 49 43 31 30 13
BmgBI CAC'GTC
Cuts at: 1318 2472 5932 9223 10627 1318
Size: 1154 3460 3291 1404 4708
Fragments arranged by size:
4708 3460 3291 1404 1154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2847 3593 4440
Size: 416 53 83 399 24 1430 746 847
Cuts at: 4440 5779 9178 10663 11748 12706 442
Size: 1339 3399 1485 1085 958 1753
Fragments arranged by size:
3399 1753 1485 1430 1339 1085 958 847
746 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 10841 1056
Size: 9785 4232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 3895 4828 7195 2833
Size: 1062 933 2367 9655
Fragments arranged by size:
9655 2367 1062 933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2372 2648 2782 3621 3865 4112 4124
Size: 839 294 276 134 839 244 247 12
Cuts at: 4124 4148 4556 4705 5120 5360 5371 5552 5732
Size: 24 408 149 415 240 11 181 180
Cuts at: 5732 5861 5993 6209 6371 6616 6803 7130 7268
Size: 129 132 216 162 245 187 327 138
Cuts at: 7268 7270 7449 7919 9389 9590 10219 10307 11174
Size: 2 179 470 1470 201 629 88 867
Cuts at: 11174 12677 1239
Size: 1503 2579
Fragments arranged by size:
2579 1503 1470 867 839 839 629 470
415 408 327 294 276 247 245 244
240 216 201 187 181 180 179 162
149 138 134 132 129 88 24 12
11 2
Bpu10I CC'TnA_GC
Cuts at: 3943 4370 4486 4526 5457 6104 6726 7577 7966
Size: 427 116 40 931 647 622 851 389
Cuts at: 7966 8340 9521 9980 10124 10208 10796 3943
Size: 374 1181 459 144 84 588 7164
Fragments arranged by size:
7164 1181 931 851 647 622 588 459
427 389 374 144 116 84 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3095 4226 4733 6971 9248 12142 13007 13305 3095
Size: 1131 507 2238 2277 2894 865 298 3807
Fragments arranged by size:
3807 2894 2277 2238 1131 865 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3896 3998 4020 4709 5152 8048 8872
Size: 617 2397 102 22 689 443 2896 824
Cuts at: 8872 10652 12680 882
Size: 1780 2028 2219
Fragments arranged by size:
2896 2397 2219 2028 1780 824 689 617
443 102 22
BsaAI yAC'GTr
Cuts at: 493 1204 10846 11314 11808 493
Size: 711 9642 468 494 2702
Fragments arranged by size:
9642 2702 711 494 468
BsaBI GATnn'nnATC
Cuts at: 14011 14011
Size: 14017
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2257 2347 2447 2880
Size: 53 83 186 936 724 90 100 433
Cuts at: 2880 3072 3689 3972 4133 5132 5190 5526 6537
Size: 192 617 283 161 999 58 336 1011
Cuts at: 6537 7253 7629 10348 11824 12206 12841 275
Size: 716 376 2719 1476 382 635 1451
Fragments arranged by size:
2719 1476 1451 1011 999 936 724 716
635 617 433 382 376 336 283 192
186 161 100 90 83 58 53
BsaWI w'CCGG_w
Cuts at: 2369 2500 2762 3253 4066 4311 4825 9042 9608
Size: 131 262 491 813 245 514 4217 566
Cuts at: 9608 9876 11033 12450 12848 13122 13269 13680 2369
Size: 268 1157 1417 398 274 147 411 2706
Fragments arranged by size:
4217 2706 1417 1157 813 566 514 491
411 398 274 268 262 245 147 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2084 2108 2114 4680
Size: 30 165 30 675 6 24 6 2566
Cuts at: 4680 4710 5333 5363 5405 5423 5435 5453 9011
Size: 30 623 30 42 18 12 18 3558
Cuts at: 9011 9041 9943 9973 10478 10508 10865 10895 11028
Size: 30 902 30 505 30 357 30 133
Cuts at: 11028 11058 11072 11102 1178
Size: 30 14 30 4093
Fragments arranged by size:
4093 3558 2566 902 675 623 505 357
165 133 42 30 30 30 30 30
30 30 30 30 30 24 18 18
14 12 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2388 2817 3041 4450 4955 4993 4996
Size: 1183 19 429 224 1409 505 38 3
Cuts at: 4996 5251 5426 5429 5432 6206 7184 7383 8078
Size: 255 175 3 3 774 978 199 695
Cuts at: 8078 8219 8222 8225 8228 8231 8234 8237 8240
Size: 141 3 3 3 3 3 3 3
Cuts at: 8240 8243 8246 8255 8268 8271 8652 8987 9509
Size: 3 3 9 13 3 381 335 522
Cuts at: 9509 9691 9968 10502 11171 1186
Size: 182 277 534 669 4032
Fragments arranged by size:
4032 1409 1183 978 774 695 669 534
522 505 429 381 335 277 255 224
199 182 175 141 38 19 13 9
3 3 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2049 2123 2267 2383 2755 3013 3541 3930 4984
Size: 74 144 116 372 258 528 389 1054
Cuts at: 4984 5046 5600 5860 6115 6375 6472 6545 6590
Size: 62 554 260 255 260 97 73 45
Cuts at: 6590 6604 6705 6879 6921 7045 7104 7220 7363
Size: 14 101 174 42 124 59 116 143
Cuts at: 7363 7487 7596 7793 7863 8450 8584 8627 8874
Size: 124 109 197 70 587 134 43 247
Cuts at: 8874 9082 9337 9895 10286 11084 11300 13220 13716
Size: 208 255 558 391 798 216 1920 496
Cuts at: 13716 2049
Size: 2350
Fragments arranged by size:
2350 1920 1054 798 587 558 554 528
496 391 389 372 260 260 258 255
255 247 216 208 197 174 144 143
134 124 124 116 116 109 101 97
74 73 70 62 59 45 43 42
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3780 3808 3831 5939 6218 7265 7698
Size: 573 2044 28 23 2108 279 1047 433
Cuts at: 7698 8537 9245 9386 9653 9965 10171 10502 10550
Size: 839 708 141 267 312 206 331 48
Cuts at: 10550 10619 10652 1163
Size: 69 33 4528
Fragments arranged by size:
4528 2108 2044 1047 839 708 573 433
331 312 279 267 206 141 69 48
33 28 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 2178 2338 2392 3191 3657
Size: 275 186 145 32 160 54 799 466
Cuts at: 3657 4121 7185 10178 11467 12228 13256 1540
Size: 464 3064 2993 1289 761 1028 2301
Fragments arranged by size:
3064 2993 2301 1289 1028 799 761 466
464 275 186 160 145 54 32
BsiHKAI G_wGCw'C
Cuts at: 4460 6198 6861 10320 12078 12163 13234 4460
Size: 1738 663 3459 1758 85 1071 5243
Fragments arranged by size:
5243 3459 1758 1738 1071 663 85
BsmI GAATG_Cn'
Cuts at: 2725 4954 7431 11528 11621 2725
Size: 2229 2477 4097 93 5121
Fragments arranged by size:
5121 4097 2477 2229 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4164 5123 10906 4164
Size: 959 5783 7275
Fragments arranged by size:
7275 5783 959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2424 2594 2709 2780 2827 2920
Size: 399 538 96 170 115 71 47 93
Cuts at: 2920 3095 3142 3495 3777 3921 3944 4460 4479
Size: 175 47 353 282 144 23 516 19
Cuts at: 4479 4926 4937 5784 5987 6018 6198 6736 6861
Size: 447 11 847 203 31 180 538 125
Cuts at: 6861 6928 7093 7603 7641 7745 8501 8514 8602
Size: 67 165 510 38 104 756 13 88
Cuts at: 8602 8645 8694 8724 10320 11055 11169 11336 12078
Size: 43 49 30 1596 735 114 167 742
Cuts at: 12078 12163 13234 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1596 1071 847 756 742 735 538
538 516 510 447 399 353 282 203
180 175 170 167 165 144 125 115
114 104 96 93 88 85 71 67
49 47 47 43 38 31 30 23
19 13 11
BspEI T'CCGG_A
Cuts at: 2369 2500 9042 9608 9876 11033 13680 2369
Size: 131 6542 566 268 1157 2647 2706
Fragments arranged by size:
6542 2706 2647 1157 566 268 131
BspHI T'CATG_A
Cuts at: 11906 13636 11906
Size: 1730 12287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2941 3574 6240 6260 9087 10147 10431 10842
Size: 2097 633 2666 20 2827 1060 284 411
Cuts at: 10842 10858 11148 11372 844
Size: 16 290 224 3489
Fragments arranged by size:
3489 2827 2666 2097 1060 633 411 290
284 224 20 16
BsrBI CCG'CTC
Cuts at: 1394 1823 2532 2999 3118 3576 3876 4750 4801
Size: 429 709 467 119 458 300 874 51
Cuts at: 4801 4807 9061 9158 9647 11904 1394
Size: 6 4254 97 489 2257 3507
Fragments arranged by size:
4254 3507 2257 874 709 489 467 458
429 300 119 97 51 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 7743 11109 12512 12686 65
Size: 1183 6495 3366 1403 174 1396
Fragments arranged by size:
6495 3366 1403 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2170 2842 4311 5226 8915 9556
Size: 110 264 96 672 1469 915 3689 641
Cuts at: 9556 9637 10350 10497 10641 12661 12848 1700
Size: 81 713 147 144 2020 187 2869
Fragments arranged by size:
3689 2869 2020 1469 915 713 672 641
264 187 147 144 110 96 81
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 14017
BssHII G'CGCG_C
Cuts at: 1925 2118 1925
Size: 193 13824
BssSI C'ACGA_G
Cuts at: 3326 3380 3434 3756 3953 12077 13089 3326
Size: 54 54 322 197 8124 1012 4254
Fragments arranged by size:
8124 4254 1012 322 197 54 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4995 6403 7042 7909 8901 9397 10834 794
Size: 4201 1408 639 867 992 496 1437 3977
Fragments arranged by size:
4201 3977 1437 1408 992 867 639 496
BstBI TT'CG_AA
Cuts at: 2007 12821 2007
Size: 10814 3203
BstEII G'GTnAC_C
Cuts at: 1184 5591 9268 10046 1184
Size: 4407 3677 778 5155
Fragments arranged by size:
5155 4407 3677 778
BstXI CCAn_nnnn'nTGG
Cuts at: 4472 6367 6713 7018 9908 10024 11047 4472
Size: 1895 346 305 2890 116 1023 7442
Fragments arranged by size:
7442 2890 1895 1023 346 305 116
BstYI r'GATC_y
Cuts at: 1972 3640 3669 5055 6486 6776 7227 7548 9009
Size: 1668 29 1386 1431 290 451 321 1461
Cuts at: 9009 10081 11347 11714 12099 12116 13568 13654 13666
Size: 1072 1266 367 385 17 1452 86 12
Cuts at: 13666 14012 1972
Size: 346 1977
Fragments arranged by size:
1977 1668 1461 1452 1431 1386 1266 1072
451 385 367 346 321 290 86 29
17 12
Bsu36I CC'TnA_GG
Cuts at: 2080 4049 4335 6573 8048 8546 8990 10521 10822
Size: 1969 286 2238 1475 498 444 1531 301
Cuts at: 10822 2080
Size: 5275
Fragments arranged by size:
5275 2238 1969 1531 1475 498 444 301
286
BtgI C'CryG_G
Cuts at: 513 1065 2022 2146 2338 3087 3221 3298 3352
Size: 552 957 124 192 749 134 77 54
Cuts at: 3352 3732 4039 5163 5550 5587 5979 7659 7764
Size: 380 307 1124 387 37 392 1680 105
Cuts at: 7764 8377 8850 8960 9247 9532 513
Size: 613 473 110 287 285 4998
Fragments arranged by size:
4998 1680 1124 957 749 613 552 473
392 387 380 307 287 285 192 134
124 110 105 77 54 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 9466 487
Size: 683 56 8240 5038
Fragments arranged by size:
8240 5038 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 3892 4465 4477 4913 5998 6839 6959 6980
Size: 1663 573 12 436 1085 841 120 21
Cuts at: 6980 7277 7445 8271 8789 10183 10664 11284 11533
Size: 297 168 826 518 1394 481 620 249
Cuts at: 11533 12327 12347 2229
Size: 794 20 3899
Fragments arranged by size:
3899 1663 1394 1085 841 826 794 620
573 518 481 436 297 249 168 120
21 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 14017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 13982
DraI TTT'AAA
Cuts at: 5688 11321 5688
Size: 5633 8384
DraIII CAC_nnn'GTG
Cuts at: 6561 8021 9779 10370 11295 6561
Size: 1460 1758 591 925 9283
Fragments arranged by size:
9283 1758 1460 925 591
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 8864 13024 809
Size: 507 4223 3325 4160 1802
Fragments arranged by size:
4223 4160 3325 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2157 2175
Size: 54 1425 50 275 18 273 54 18
Cuts at: 2175 2215 2335 2389 2844 3188 3571 4118 4603
Size: 40 120 54 455 344 383 547 485
Cuts at: 4603 4645 4968 5140 5196 5734 6033 6501 6839
Size: 42 323 172 56 538 299 468 338
Cuts at: 6839 7119 7182 7815 8169 8367 8380 8441 8577
Size: 280 63 633 354 198 13 61 136
Cuts at: 8577 8740 9153 9561 10175 10779 11464 12353 13982
Size: 163 413 408 614 604 685 889 1629
Cuts at: 13982 8
Size: 43
Fragments arranged by size:
1629 1425 889 685 633 614 604 547
538 485 468 455 413 408 383 354
344 338 323 299 280 275 273 198
172 163 136 120 63 61 56 54
54 54 50 43 42 40 18 18
13
EagI C'GGCC_G
Cuts at: 1537 1812 2175 2335 2389 3188 4118 7182 10175
Size: 275 363 160 54 799 930 3064 2993
Cuts at: 10175 11464 1537
Size: 1289 4090
Fragments arranged by size:
4090 3064 2993 1289 930 799 363 275
160 54
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 4241 5225 5387 5567 9377 10550 10556
Size: 1020 1868 984 162 180 3810 1173 6
Cuts at: 10556 10988 11114 11947 1353
Size: 432 126 833 3423
Fragments arranged by size:
3810 3423 1868 1173 1020 984 833 432
180 162 126 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3908 6536 9079 9278 10369 12604
Size: 1064 138 2319 2628 2543 199 1091 2235
Cuts at: 12604 12978 13124 387
Size: 374 146 1280
Fragments arranged by size:
2628 2543 2319 2235 1280 1091 1064 374
199 146 138
EcoICRI GAG'CTC
Cuts at: 6196 6859 6196
Size: 663 13354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2372 2648 2782 3621 3729 3865
Size: 393 446 294 276 134 839 108 136
Cuts at: 3865 4112 4124 4148 4556 4705 4928 5120 5360
Size: 247 12 24 408 149 223 192 240
Cuts at: 5360 5371 5552 5732 5861 5993 6209 6371 6533
Size: 11 181 180 129 132 216 162 162
Cuts at: 6533 6616 6803 7130 7147 7268 7270 7449 7919
Size: 83 187 327 17 121 2 179 470
Cuts at: 7919 8091 8956 9323 9389 9401 9590 10121 10219
Size: 172 865 367 66 12 189 531 98
Cuts at: 10219 10307 10712 11138 11174 12080 12677 13464 13732
Size: 88 405 426 36 906 597 787 268
Cuts at: 13732 1239
Size: 1524
Fragments arranged by size:
1524 906 865 839 787 597 531 470
446 426 408 405 393 367 327 294
276 268 247 240 223 216 192 189
187 181 180 179 172 162 162 149
136 134 132 129 121 108 98 88
83 66 36 24 17 12 12 11
2
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 3759 4016 4538 4847 5198 5768 6107 6164
Size: 1405 257 522 309 351 570 339 57
Cuts at: 6164 6793 8942 10799 2354
Size: 629 2149 1857 5572
Fragments arranged by size:
5572 2149 1857 1405 629 570 522 351
339 309 257 57
EcoO109I rG'GnC_Cy
Cuts at: 2420 2421 2705 2706 2802 3010 3060 3092 3586
Size: 1 284 1 96 208 50 32 494
Cuts at: 3586 3947 3959 4044 4099 4192 4283 4366 4438
Size: 361 12 85 55 93 91 83 72
Cuts at: 4438 5320 5836 5845 6046 6157 6220 6444 6925
Size: 882 516 9 201 111 63 224 481
Cuts at: 6925 7006 7050 7351 7360 7438 7457 8079 8498
Size: 81 44 301 9 78 19 622 419
Cuts at: 8498 8641 8642 8674 8691 8720 8721 8806 8814
Size: 143 1 32 17 29 1 85 8
Cuts at: 8814 9278 9538 9738 9882 10283 10831 11026 11165
Size: 464 260 200 144 401 548 195 139
Cuts at: 11165 11166 2420
Size: 1 5271
Fragments arranged by size:
5271 882 622 548 516 494 481 464
419 401 361 301 284 260 224 208
201 200 195 144 143 139 111 96
93 91 85 85 83 81 78 72
63 55 50 44 32 32 29 19
17 12 9 9 8 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 9857 10535 11326 11704 9857
Size: 678 791 378 12170
Fragments arranged by size:
12170 791 678 378
EcoRV GAT'ATC
Cuts at: 7566 7566
Size: 14017
FseI GG_CCGG'CC
Cuts at: 2078 2846 2078
Size: 768 13249
FspI TGC'GCA
Cuts at: 8417 9491 12523 8417
Size: 1074 3032 9911
Fragments arranged by size:
9911 3032 1074
HaeII r_GCGC'y
Cuts at: 1678 2048 2056 2350 2627 2883 3075 3975 4136
Size: 370 8 294 277 256 192 900 161
Cuts at: 4136 5374 6214 6481 6951 7028 7148 7256 7632
Size: 1238 840 267 470 77 120 108 376
Cuts at: 7632 7853 8786 9434 9751 10351 10752 13164 1678
Size: 221 933 648 317 600 401 2412 2531
Fragments arranged by size:
2531 2412 1238 933 900 840 648 600
470 401 376 370 317 294 277 267
256 221 192 161 120 108 77 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2084 2110 2116 2576
Size: 32 135 32 506 6 26 6 460
Cuts at: 2576 2608 2729 2761 3625 3657 5003 5035 5423
Size: 32 121 32 864 32 1346 32 388
Cuts at: 5423 5455 5566 5598 5771 5803 6274 6306 7178
Size: 32 111 32 173 32 471 32 872
Cuts at: 7178 7210 7934 7966 8015 8047 9011 9043 9725
Size: 32 724 32 49 32 964 32 682
Cuts at: 9725 9757 11028 11060 11072 11104 12658 12690 12732
Size: 32 1271 32 12 32 1554 32 42
Cuts at: 12732 12764 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 1346 1271 964 872 864 724
682 506 471 460 388 173 135 121
111 49 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 26 12
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 6229 10239 11185 11361 11607 669
Size: 988 281 4291 4010 946 176 246 3079
Fragments arranged by size:
4291 4010 3079 988 946 281 246 176
HindIII A'AGCT_T
Cuts at: 748 5912 8538 748
Size: 5164 2626 6227
Fragments arranged by size:
6227 5164 2626
HpaI GTT'AAC
Cuts at: 11607 11607
Size: 14017
KasI G'GCGC_C
Cuts at: 2346 2879 3071 3971 4132 7252 7628 10347 2346
Size: 533 192 900 161 3120 376 2719 6016
Fragments arranged by size:
6016 3120 2719 900 533 376 192 161
KpnI G_GTAC'C
Cuts at: 3216 5087 6325 11342 3216
Size: 1871 1238 5017 5891
Fragments arranged by size:
5891 5017 1871 1238
MfeI C'AATT_G
Cuts at: 11616 11616
Size: 14017
MluI A'CGCG_T
Cuts at: 13893 13893
Size: 14017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 2766 2914 3879 9729 13131 13315 1305
Size: 861 600 148 965 5850 3402 184 2007
Fragments arranged by size:
5850 3402 2007 965 861 600 184 148
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4605 5142 5198 5736 6035
Size: 54 1425 343 2773 537 56 538 299
Cuts at: 6035 6841 8382 8579 8742 9563 10
Size: 806 1541 197 163 821 4464
Fragments arranged by size:
4464 2773 1541 1425 821 806 538 537
343 299 197 163 56 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4470 5092 5297 5589 7724 7769 8477
Size: 648 3304 622 205 292 2135 45 708
Cuts at: 8477 9149 11075 11975 12334 12493 518
Size: 672 1926 900 359 159 2042
Fragments arranged by size:
3304 2135 2042 1926 900 708 672 648
622 359 292 205 159 45
MspA1I CmG'CkG
Cuts at: 1606 2148 2340 2755 2875 2960 3022 3300 3354
Size: 542 192 415 120 85 62 278 54
Cuts at: 3354 4984 5707 5752 6184 6352 6709 6871 7069
Size: 1630 723 45 432 168 357 162 198
Cuts at: 7069 7108 7216 7813 7918 8366 8654 9229 9301
Size: 39 108 597 105 448 288 575 72
Cuts at: 9301 9394 9700 10570 10858 10975 11131 11198 11433
Size: 93 306 870 288 117 156 67 235
Cuts at: 11433 12110 13258 13503 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1630 1148 870 723 677 597 575
542 448 432 415 357 306 288 288
278 245 235 198 192 168 162 156
120 117 108 105 93 85 72 67
62 54 45 39
NaeI GCC'GGC
Cuts at: 2076 2844 9639 10352 10499 10643 2076
Size: 768 6795 713 147 144 5450
Fragments arranged by size:
6795 5450 768 713 147 144
NarI GG'CG_CC
Cuts at: 2347 2880 3072 3972 4133 7253 7629 10348 2347
Size: 533 192 900 161 3120 376 2719 6016
Fragments arranged by size:
6016 3120 2719 900 533 376 192 161
NcoI C'CATG_G
Cuts at: 513 1065 2022 5587 7659 7764 8377 513
Size: 552 957 3565 2072 105 613 6153
Fragments arranged by size:
6153 3565 2072 957 613 552 105
NdeI CA'TA_TG
Cuts at: 387 387
Size: 14017
NgoMIV G'CCGG_C
Cuts at: 2074 2842 9637 10350 10497 10641 2074
Size: 768 6795 713 147 144 5450
Fragments arranged by size:
6795 5450 768 713 147 144
NheI G'CTAG_C
Cuts at: 1052 10837 1052
Size: 9785 4232
NotI GC'GGCC_GC
Cuts at: 2335 2389 3188 11464 2335
Size: 54 799 8276 4888
Fragments arranged by size:
8276 4888 799 54
NruI TCG'CGA
Cuts at: 3056 5152 3056
Size: 2096 11921
NsiI A_TGCA'T
Cuts at: 4448 4448
Size: 14017
NspI r_CATG'y
Cuts at: 1132 1229 5942 6298 8482 11023 11375 12920 1132
Size: 97 4713 356 2184 2541 352 1545 2229
Fragments arranged by size:
4713 2541 2229 2184 1545 356 352 97
PasI CC'CwG_GG
Cuts at: 1348 2261 2328 2710 3142 6049 7011 8188 8810
Size: 913 67 382 432 2907 962 1177 622
Cuts at: 8810 1348
Size: 6555
Fragments arranged by size:
6555 2907 1177 962 913 622 432 382
67
PciI A'CATG_T
Cuts at: 5938 12916 5938
Size: 6978 7039
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2056 2267 3088 5029 5755 6049 6115 6223
Size: 948 211 821 1941 726 294 66 108
Cuts at: 6223 6874 8128 10297 1108
Size: 651 1254 2169 4828
Fragments arranged by size:
4828 2169 1941 1254 948 821 726 651
294 211 108 66
PfoI T'CCnGG_A
Cuts at: 2798 5520 5769 6198 7539 9966 2798
Size: 2722 249 429 1341 2427 6849
Fragments arranged by size:
6849 2722 2427 1341 429 249
PmeI GTTT'AAAC
Cuts at: 11321 11321
Size: 14017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10865 10897 12086 12118 13599 13631 10865
Size: 32 1189 32 1481 32 11251
Fragments arranged by size:
11251 1481 1189 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2802 3010 3586 3959 4044 4099 4192 4438 5845
Size: 208 576 373 85 55 93 246 1407
Cuts at: 5845 6157 7351 7360 7457 8806 8814 9538 9882
Size: 312 1194 9 97 1349 8 724 344
Cuts at: 9882 10831 2802
Size: 949 5988
Fragments arranged by size:
5988 1407 1349 1194 949 724 576 373
344 312 246 208 97 93 85 55
9 8
PshAI GACnn'nnGTC
Cuts at: 1613 2748 3342 1613
Size: 1135 594 12288
Fragments arranged by size:
12288 1135 594
PsiI TTA'TAA
Cuts at: 11587 11587
Size: 14017
PspOMI G'GGCC_C
Cuts at: 2324 2420 2705 3091 6924 7089 7637 8497 8641
Size: 96 285 386 3833 165 548 860 144
Cuts at: 8641 8690 8720 11165 2324
Size: 49 30 2445 5176
Fragments arranged by size:
5176 3833 2445 860 548 386 285 165
144 96 49 30
PspXI vC'TCGA_Gb
Cuts at: 2746 10191 2746
Size: 7445 6572
PstI C_TGCA'G
Cuts at: 830 2236 2255 4017 4472 4821 4865 4920 4983
Size: 1406 19 1762 455 349 44 55 63
Cuts at: 4983 6168 6408 7236 9515 10275 10587 10791 11130
Size: 1185 240 828 2279 760 312 204 339
Cuts at: 11130 11369 830
Size: 239 3478
Fragments arranged by size:
3478 2279 1762 1406 1185 828 760 455
349 339 312 240 239 204 63 55
44 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14017
PvuII CAG'CTG
Cuts at: 4984 5707 5752 6352 6709 6871 7069 7108 7216
Size: 723 45 600 357 162 198 39 108
Cuts at: 7216 7813 8654 9229 9301 9394 9700 10570 10858
Size: 597 841 575 72 93 306 870 288
Cuts at: 10858 10975 11198 4984
Size: 117 223 7803
Fragments arranged by size:
7803 870 841 723 600 597 575 357
306 288 223 198 162 117 108 93
72 45 39
SacI G_AGCT'C
Cuts at: 6198 6861 6198
Size: 663 13354
SacII CC_GC'GG
Cuts at: 2149 2341 3301 3355 2149
Size: 192 960 54 12811
Fragments arranged by size:
12811 960 192 54
SalI G'TCGA_C
Cuts at: 1936 6227 10237 11359 1936
Size: 4291 4010 1122 4594
Fragments arranged by size:
4594 4291 4010 1122
SanDI GG'GwC_CC
Cuts at: 4044 7360 8814 4044
Size: 3316 1454 9247
Fragments arranged by size:
9247 3316 1454
SapI GCTCTTCn'nnn_
Cuts at: 2373 5387 5567 9377 10556 11114 2373
Size: 3014 180 3810 1179 558 5276
Fragments arranged by size:
5276 3810 3014 1179 558 180
SbfI CC_TGCA'GG
Cuts at: 4821 4865 11369 4821
Size: 44 6504 7469
Fragments arranged by size:
7469 6504 44
ScaI AGT'ACT
Cuts at: 12265 13977 12265
Size: 1712 12305
SexAI A'CCwGG_T
Cuts at: 2928 5670 6816 2928
Size: 2742 1146 10129
Fragments arranged by size:
10129 2742 1146
SfcI C'TryA_G
Cuts at: 826 1045 2232 2251 4013 4468 4667 4817 4861
Size: 219 1187 19 1762 455 199 150 44
Cuts at: 4861 4916 4979 5375 6164 6404 6971 7232 8681
Size: 55 63 396 789 240 567 261 1449
Cuts at: 8681 9511 10011 10271 10583 10702 10787 11126 11365
Size: 830 500 260 312 119 85 339 239
Cuts at: 11365 12500 13181 13372 826
Size: 1135 681 191 1471
Fragments arranged by size:
1762 1471 1449 1187 1135 830 789 681
567 500 455 396 339 312 261 260
240 239 219 199 191 150 119 85
63 55 44 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 2071 2909 6032 7079 7088 2071
Size: 838 3123 1047 9 9000
Fragments arranged by size:
9000 3123 1047 838 9
SfoI GGC'GCC
Cuts at: 2348 2881 3073 3973 4134 7254 7630 10349 2348
Size: 533 192 900 161 3120 376 2719 6016
Fragments arranged by size:
6016 3120 2719 900 533 376 192 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14017
SgrAI Cr'CCGG_yG
Cuts at: 1700 10350 1700
Size: 8650 5367
SmaI CCC'GGG
Cuts at: 1872 2068 2555 2678 2915 3496 4032 7262 8496
Size: 196 487 123 237 581 536 3230 1234
Cuts at: 8496 9413 10064 11344 1872
Size: 917 651 1280 4545
Fragments arranged by size:
4545 3230 1280 1234 917 651 581 536
487 237 196 123
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2746 3110 4241 4712 6986 9263
Size: 197 926 803 364 1131 471 2274 2277
Cuts at: 9263 9458 10191 12121 13022 13284 820
Size: 195 733 1930 901 262 1553
Fragments arranged by size:
2277 2274 1930 1553 1131 926 901 803
733 471 364 262 197 195
SnaBI TAC'GTA
Cuts at: 493 11314 11808 493
Size: 10821 494 2702
Fragments arranged by size:
10821 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 14017
SphI G_CATG'C
Cuts at: 1132 6298 11023 11375 1132
Size: 5166 4725 352 3774
Fragments arranged by size:
5166 4725 3774 352
SrfI GCCC'GGGC
Cuts at: 2915 3496 8496 9413 2915
Size: 581 5000 917 7519
Fragments arranged by size:
7519 5000 917 581
SspI AAT'ATT
Cuts at: 5 52 11941 5
Size: 47 11889 2081
Fragments arranged by size:
11889 2081 47
StuI AGG'CCT
Cuts at: 1517 2903 4859 5922 6026 7245 7958 8530 9890
Size: 1386 1956 1063 104 1219 713 572 1360
Cuts at: 9890 1517
Size: 5644
Fragments arranged by size:
5644 1956 1386 1360 1219 1063 713 572
104
StyI C'CwwG_G
Cuts at: 513 1065 2022 2847 3962 3999 4152 5199 5587
Size: 552 957 825 1115 37 153 1047 388
Cuts at: 5587 6142 6153 6447 7053 7659 7764 8172 8355
Size: 555 11 294 606 606 105 408 183
Cuts at: 8355 8370 8377 8383 8395 8562 8670 9274 9666
Size: 15 7 6 12 167 108 604 392
Cuts at: 9666 9761 9954 10752 11456 513
Size: 95 193 798 704 3074
Fragments arranged by size:
3074 1115 1047 957 825 798 704 606
606 604 555 552 408 392 388 294
193 183 167 153 108 105 95 37
15 12 11 7 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5434 7212 7732 8888 9329 10034 10712
Size: 1255 3854 1778 520 1156 441 705 678
Cuts at: 10712 12044 12061 12214 12399 12844 325
Size: 1332 17 153 185 445 1498
Fragments arranged by size:
3854 1778 1498 1332 1255 1156 705 678
520 445 441 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 9948 11100 12263
Size: 274 80 33 51 1443 7971 1152 1163
Cuts at: 12263 13975 96
Size: 1712 138
Fragments arranged by size:
7971 1712 1443 1163 1152 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2928 2960 4323 4355 4680 4712 5333 5365 5405
Size: 32 1363 32 325 32 621 32 40
Cuts at: 5405 5437 6951 6983 9943 9975 10478 10510 13977
Size: 32 1514 32 2960 32 503 32 3467
Cuts at: 13977 14009 2928
Size: 32 2936
Fragments arranged by size:
3467 2960 2936 1514 1363 621 503 325
40 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 9131 1316
Size: 7815 6202
XbaI T'CTAG_A
Cuts at: 11353 11353
Size: 14017
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2207 4981 5191 5251 5585 6367 7762 9430 10294
Size: 2774 210 60 334 782 1395 1668 864
Cuts at: 10294 2207
Size: 5930
Fragments arranged by size:
5930 2774 1668 1395 864 782 334 210
60
XhoI C'TCGA_G
Cuts at: 1943 2746 10191 1943
Size: 803 7445 5769
Fragments arranged by size:
7445 5769 803
XmaI C'CCGG_G
Cuts at: 1870 2066 2553 2676 2913 3494 4030 7260 8494
Size: 196 487 123 237 581 536 3230 1234
Cuts at: 8494 9411 10062 11342 1870
Size: 917 651 1280 4545
Fragments arranged by size:
4545 3230 1280 1234 917 651 581 536
487 237 196 123
XmnI GAAnn'nnTTC
Cuts at: 8117 8933 10382 12146 8117
Size: 816 1449 1764 9988
Fragments arranged by size:
9988 1764 1449 816
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11825 276
Size: 53 83 186 936 10291 2468
Fragments arranged by size:
10291 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
BaeI BsiWI BstZ17I FalI FspAI PacI PmlI PsrI
RsrII SwaI