Restriction map for
pFN21ASDA1759
(Circular) (Six-base) MAPSORT of: pFN21ASDA1759.seq Check: 740 from: 1 to: 7645
pFN21ASDA1759 7645 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3918 4405 3918
Size: 487 7158
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5455 278
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2077 4797 4988 1937
Size: 140 2720 191 4594
Fragments arranged by size:
4594 2720 191 140
Acc65I G'GTAC_C
Cuts at: 4966 4966
Size: 7645
AclI AA'CG_TT
Cuts at: 1563 5772 6145 1563
Size: 4209 373 3063
Fragments arranged by size:
4209 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3223 4652 4872 5708 7092 7360 1632
Size: 1591 1429 220 836 1384 268 1917
Fragments arranged by size:
1917 1591 1429 1384 836 268 220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7448
AflIII A'CryG_T
Cuts at: 1203 6544 7521 1203
Size: 5341 977 1327
Fragments arranged by size:
5341 1327 977
AgeI A'CCGG_T
Cuts at: 6476 6476
Size: 7645
AhdI GACnn_n'nnGTC
Cuts at: 6374 6374
Size: 7645
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3830 4214 1184
Size: 2646 384 4615
Fragments arranged by size:
4615 2646 384
AlwNI CAG_nnn'CTG
Cuts at: 2201 2300 2455 3113 3553 4351 4420 4553 5388
Size: 99 155 658 440 798 69 133 835
Cuts at: 5388 6960 2201
Size: 1572 2886
Fragments arranged by size:
2886 1572 835 798 658 440 155 133
99 69
ApaI G_GGCC'C
Cuts at: 2066 2307 3902 4541 4645 4756 4929 2066
Size: 241 1595 639 104 111 173 4782
Fragments arranged by size:
4782 1595 639 241 173 111 104
ApaLI G'TGCA_C
Cuts at: 5702 6858 5702
Size: 1156 6489
ApoI r'AATT_y
Cuts at: 1493 4820 4954 5180 5332 7441 1493
Size: 3327 134 226 152 2109 1697
Fragments arranged by size:
3327 2109 1697 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 6199 160
Size: 872 5167 1606
Fragments arranged by size:
5167 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2120 2190 2603 4268 4743 4970
Size: 73 115 62 70 413 1665 475 227
Cuts at: 4970 1870
Size: 4545
Fragments arranged by size:
4545 1665 475 413 227 115 73 70
62
BamHI G'GATC_C
Cuts at: 4975 5342 4975
Size: 367 7278
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2693 3016 3408 3976
Size: 325 217 627 283 623 323 392 568
Cuts at: 3976 4966 6421 618
Size: 990 1455 1842
Fragments arranged by size:
1842 1455 990 627 623 568 392 325
323 283 217
BanII G_rGCy'C
Cuts at: 1391 2066 2307 2756 2817 3777 3902 4056 4173
Size: 675 241 449 61 960 125 154 117
Cuts at: 4173 4427 4541 4645 4712 4756 4929 4964 1391
Size: 254 114 104 67 44 173 35 4072
Fragments arranged by size:
4072 960 675 449 254 241 173 154
125 117 114 104 67 61 44 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2131 2642 2895 3672 4378 928
Size: 971 232 511 253 777 706 4195
Fragments arranged by size:
4195 971 777 706 511 253 232
BbvCI CC'TCA_GC
Cuts at: 3257 4187 4533 3257
Size: 930 346 6369
Fragments arranged by size:
6369 930 346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2204 2205 2238 2265 2299 5836
Size: 34 234 33 1 33 27 34 3537
Cuts at: 5836 5870 1903
Size: 34 3678
Fragments arranged by size:
3678 3537 234 34 34 34 33 33
27 1
BciVI GTATCCnnnnn_n'
Cuts at: 5537 6747 5537
Size: 1210 6435
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3449 3558 4331
Size: 107 122 71 631 605 1777 109 773
Cuts at: 4331 4649 6256 136
Size: 318 1607 1525
Fragments arranged by size:
1777 1607 1525 773 631 605 318 122
109 107 71
BglII A'GATC_T
Cuts at: 7640 7640
Size: 7645
BlpI GC'TnA_GC
Cuts at: 2042 2406 3323 5062 2042
Size: 364 917 1739 4625
Fragments arranged by size:
4625 1739 917 364
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2073 2307 2523 2696 2824 3454 3902
Size: 276 7 234 216 173 128 630 448
Cuts at: 3902 4541 4645 4756 4929 5706 6862 1790
Size: 639 104 111 173 777 1156 2573
Fragments arranged by size:
2573 1156 777 639 630 448 276 234
216 173 173 128 111 104 7
BmgBI CAC'GTC
Cuts at: 1318 3698 1318
Size: 2380 5265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2517 3771
Size: 416 53 83 399 24 682 418 1254
Cuts at: 3771 4389 4537 5376 6334 442
Size: 618 148 839 958 1753
Fragments arranged by size:
1753 1254 958 839 682 618 418 416
399 148 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2460 3460 3781 4030 2460
Size: 1000 321 249 6075
Fragments arranged by size:
6075 1000 321 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2333 2655 2954 3555 3601 3675 3698
Size: 956 138 322 299 601 46 74 23
Cuts at: 3698 4713 6305 1239
Size: 1015 1592 2579
Fragments arranged by size:
2579 1592 1015 956 601 322 299 138
74 46 23
Bpu10I CC'TnA_GC
Cuts at: 2628 3257 3771 4167 4187 4533 2628
Size: 629 514 396 20 346 5740
Fragments arranged by size:
5740 629 514 396 346 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2067 4148 4811 5770 6635 6933 2067
Size: 2081 663 959 865 298 2779
Fragments arranged by size:
2779 2081 959 865 663 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 3229 6308 882
Size: 617 618 1112 3079 2219
Fragments arranged by size:
3079 2219 1112 618 617
BsaAI yAC'GTr
Cuts at: 493 1204 4942 5436 493
Size: 711 3738 494 2702
Fragments arranged by size:
3738 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7639 7639
Size: 7645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5452 5834 6469 275
Size: 53 83 186 936 3919 382 635 1451
Fragments arranged by size:
3919 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6078 6476 6750 6897 7308 6078
Size: 398 274 147 411 6415
Fragments arranged by size:
6415 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7420
Fragments arranged by size:
7420 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3071 3472 3662 3836 3866 3883 4145 4148
Size: 1885 401 190 174 30 17 262 3
Cuts at: 4148 4151 1186
Size: 3 4680
Fragments arranged by size:
4680 1885 401 262 190 174 30 17
3 3
BseYI C'CCAG_C
Cuts at: 2530 2747 2896 3419 3577 3862 3881 3921 3992
Size: 217 149 523 158 285 19 40 71
Cuts at: 3992 4326 4344 4420 4474 6848 7344 2530
Size: 334 18 76 54 2374 496 2831
Fragments arranged by size:
2831 2374 523 496 334 285 217 158
149 76 71 54 40 19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2229 2295 2309 2996 3909 4824 1163
Size: 573 493 66 14 687 913 915 3984
Fragments arranged by size:
3984 915 913 687 573 493 66 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5095 5856 6884 1540
Size: 275 186 3094 761 1028 2301
Fragments arranged by size:
3094 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4056 4257 5706 5791 6862 4056
Size: 201 1449 85 1071 4839
Fragments arranged by size:
4839 1449 1071 201 85
BsmI GAATG_Cn'
Cuts at: 4795 5156 5249 4795
Size: 361 93 7191
Fragments arranged by size:
7191 361 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4674 4674
Size: 7645
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2073 2307 2523 2696 2756 2817
Size: 399 276 7 234 216 173 60 61
Cuts at: 2817 2824 3454 3777 3902 4056 4173 4257 4427
Size: 7 630 323 125 154 117 84 170
Cuts at: 4427 4541 4645 4712 4756 4929 4964 5706 5791
Size: 114 104 67 44 173 35 742 85
Cuts at: 5791 6862 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 630 399 323 276 234
216 173 173 170 154 125 117 114
104 85 84 67 61 60 44 35
7 7
BspEI T'CCGG_A
Cuts at: 7308 7308
Size: 7645
BspHI T'CATG_A
Cuts at: 2922 5534 7264 2922
Size: 2612 1730 3303
Fragments arranged by size:
3303 2612 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 3918 4405 4561 5000 844
Size: 1291 1783 487 156 439 3489
Fragments arranged by size:
3489 1783 1291 487 439 156
BsrBI CCG'CTC
Cuts at: 1394 1823 2179 2642 3760 4359 5532 1394
Size: 429 356 463 1118 599 1173 3507
Fragments arranged by size:
3507 1173 1118 599 463 429 356
BsrDI GCAATG_nn'
Cuts at: 65 1248 2372 2597 2950 4087 4506 6140 6314
Size: 1183 1124 225 353 1137 419 1634 174
Cuts at: 6314 65
Size: 1396
Fragments arranged by size:
1634 1396 1183 1137 1124 419 353 225
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3153 3334 6289 6476 1700
Size: 110 1343 181 2955 187 2869
Fragments arranged by size:
2955 2869 1343 187 181 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7645
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7645
BssSI C'ACGA_G
Cuts at: 2327 3453 5705 6717 2327
Size: 1126 2252 1012 3255
Fragments arranged by size:
3255 2252 1126 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2878 4553 794
Size: 2084 1675 3886
Fragments arranged by size:
3886 2084 1675
BstBI TT'CG_AA
Cuts at: 2007 6449 2007
Size: 4442 3203
BstEII G'GTnAC_C
Cuts at: 1184 3905 1184
Size: 2721 4924
BstXI CCAn_nnnn'nTGG
Cuts at: 3929 3945 3929
Size: 16 7629
BstYI r'GATC_y
Cuts at: 1972 2332 4898 4975 5342 5727 5744 7196 7282
Size: 360 2566 77 367 385 17 1452 86
Cuts at: 7282 7294 7640 1972
Size: 12 346 1977
Fragments arranged by size:
2566 1977 1452 385 367 360 346 86
77 17 12
BstZ17I GTA'TAC
Cuts at: 2078 2078
Size: 7645
Bsu36I CC'TnA_GG
Cuts at: 3819 3951 3819
Size: 132 7513
BtgI C'CryG_G
Cuts at: 513 1065 2022 4513 513
Size: 552 957 2491 3645
Fragments arranged by size:
3645 2491 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2758 487
Size: 683 56 1532 5374
Fragments arranged by size:
5374 1532 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 2834 4118 4478 5161 5955 5975 2255
Size: 579 1284 360 683 794 20 3925
Fragments arranged by size:
3925 1284 794 683 579 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7645
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7610
DraI TTT'AAA
Cuts at: 4949 4949
Size: 7645
DraIII CAC_nnn'GTG
Cuts at: 3701 3701
Size: 7645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3716 6652 809
Size: 507 2400 2936 1802
Fragments arranged by size:
2936 2400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 2805 3625
Size: 54 1425 50 275 18 679 296 820
Cuts at: 3625 5092 5981 7610 8
Size: 1467 889 1629 43
Fragments arranged by size:
1629 1467 1425 889 820 679 296 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5092 1537
Size: 275 3280 4090
Fragments arranged by size:
4090 3280 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2924 3242 3490 3539 3852 4577 4782 5575
Size: 1571 318 248 49 313 725 205 793
Cuts at: 5575 1353
Size: 3423
Fragments arranged by size:
3423 1571 793 725 318 313 248 205
49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6232 6606 6752 387
Size: 1064 138 4643 374 146 1280
Fragments arranged by size:
4643 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4054 4054
Size: 7645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2333 2655 2954 3223 3555 3601
Size: 393 563 138 322 299 269 332 46
Cuts at: 3601 3675 3698 4652 4713 4872 5708 6305 7092
Size: 74 23 954 61 159 836 597 787
Cuts at: 7092 7360 1239
Size: 268 1524
Fragments arranged by size:
1524 954 836 787 597 563 393 332
322 299 269 268 159 138 74 61
46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 4130 4412 4545 4130
Size: 282 133 7230
Fragments arranged by size:
7230 282 133
EcoO109I rG'GnC_Cy
Cuts at: 2062 2063 2186 2297 2304 3029 3243 3458 3898
Size: 1 123 111 7 725 214 215 440
Cuts at: 3898 3899 4537 4538 4550 4641 4752 4753 4926
Size: 1 638 1 12 91 111 1 173
Cuts at: 4926 2062
Size: 4781
Fragments arranged by size:
4781 725 638 440 215 214 173 123
111 111 91 12 7 1 1 1
1
EcoRI G'AATT_C
Cuts at: 4954 5332 4954
Size: 378 7267
FspI TGC'GCA
Cuts at: 2231 6151 2231
Size: 3920 3725
HaeII r_GCGC'y
Cuts at: 1678 6792 1678
Size: 5114 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4883 4915 6286 6318 6360
Size: 32 135 32 3311 32 1371 32 42
Cuts at: 6360 6392 1373
Size: 32 2626
Fragments arranged by size:
3311 2626 1371 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2253 2352 4300 4989 5235 669
Size: 988 281 315 99 1948 689 246 3079
Fragments arranged by size:
3079 1948 988 689 315 281 246 99
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7645
HpaI GTT'AAC
Cuts at: 5235 5235
Size: 7645
KpnI G_GTAC'C
Cuts at: 4970 4970
Size: 7645
MfeI C'AATT_G
Cuts at: 4658 5244 4658
Size: 586 7059
MluI A'CGCG_T
Cuts at: 7521 7521
Size: 7645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4503 4658 4860 6759 6943 1305
Size: 3198 155 202 1899 184 2007
Fragments arranged by size:
3198 2007 1899 202 184 155
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2807 10
Size: 54 1425 343 975 4848
Fragments arranged by size:
4848 1425 975 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3156 4250 4379 5603 5962 6121 518
Size: 648 1990 1094 129 1224 359 159 2042
Fragments arranged by size:
2042 1990 1224 1094 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2362 2569 2747 3261 3283 3371 3714 4216
Size: 756 207 178 514 22 88 343 502
Cuts at: 4216 4330 5061 5738 6886 7131 1606
Size: 114 731 677 1148 245 2120
Fragments arranged by size:
2120 1148 756 731 677 514 502 343
245 207 178 114 88 22
NaeI GCC'GGC
Cuts at: 3336 3336
Size: 7645
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6136
Fragments arranged by size:
6136 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7645
NgoMIV G'CCGG_C
Cuts at: 3334 3334
Size: 7645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7645
NotI GC'GGCC_GC
Cuts at: 5092 5092
Size: 7645
NspI r_CATG'y
Cuts at: 1132 1229 3443 4279 4625 5003 6548 1132
Size: 97 2214 836 346 378 1545 2229
Fragments arranged by size:
2229 2214 1545 836 378 346 97
PasI CC'CwG_GG
Cuts at: 1348 2066 2300 2698 4715 1348
Size: 718 234 398 2017 4278
Fragments arranged by size:
4278 2017 718 398 234
PciI A'CATG_T
Cuts at: 6544 6544
Size: 7645
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7645
PfoI T'CCnGG_A
Cuts at: 2172 3460 3791 4056 2172
Size: 1288 331 265 5761
Fragments arranged by size:
5761 1288 331 265
PmeI GTTT'AAAC
Cuts at: 4949 4949
Size: 7645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5714 5746 7227 7259 5714
Size: 32 1481 32 6100
Fragments arranged by size:
6100 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3029 3243 3458 4550 3029
Size: 214 215 1092 6124
Fragments arranged by size:
6124 1092 215 214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7645
PsiI TTA'TAA
Cuts at: 5215 5215
Size: 7645
PspOMI G'GGCC_C
Cuts at: 2062 2303 3898 4537 4641 4752 4925 2062
Size: 241 1595 639 104 111 173 4782
Fragments arranged by size:
4782 1595 639 241 173 111 104
PspXI vC'TCGA_Gb
Cuts at: 2603 2603
Size: 7645
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3078 3110 3078
Size: 32 7613
PstI C_TGCA'G
Cuts at: 830 2619 3282 3646 4284 4416 4546 4997 830
Size: 1789 663 364 638 132 130 451 3478
Fragments arranged by size:
3478 1789 663 638 451 364 132 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7645
PvuII CAG'CTG
Cuts at: 2362 2569 2747 3261 3283 3371 4216 4330 2362
Size: 207 178 514 22 88 845 114 5677
Fragments arranged by size:
5677 845 514 207 178 114 88 22
SacI G_AGCT'C
Cuts at: 4056 4056
Size: 7645
SalI G'TCGA_C
Cuts at: 1936 4987 1936
Size: 3051 4594
SapI GCTCTTCn'nnn_
Cuts at: 3852 4577 4782 3852
Size: 725 205 6715
Fragments arranged by size:
6715 725 205
SbfI CC_TGCA'GG
Cuts at: 4997 4997
Size: 7645
ScaI AGT'ACT
Cuts at: 5893 7605 5893
Size: 1712 5933
SexAI A'CCwGG_T
Cuts at: 3734 3734
Size: 7645
SfcI C'TryA_G
Cuts at: 826 1045 2615 3278 3642 4280 4412 4542 4993
Size: 219 1570 663 364 638 132 130 451
Cuts at: 4993 6128 6809 7000 826
Size: 1135 681 191 1471
Fragments arranged by size:
1570 1471 1135 681 663 638 451 364
219 191 132 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 4331 4331
Size: 7645
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7645
SmaI CCC'GGG
Cuts at: 1872 2060 4745 4972 1872
Size: 188 2685 227 4545
Fragments arranged by size:
4545 2685 227 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2082 2603 4127 4268 4826 5749
Size: 197 926 139 521 1524 141 558 923
Cuts at: 5749 6650 6912 820
Size: 901 262 1553
Fragments arranged by size:
1553 1524 926 923 901 558 521 262
197 141 139
SnaBI TAC'GTA
Cuts at: 493 4942 5436 493
Size: 4449 494 2702
Fragments arranged by size:
4449 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7645
SphI G_CATG'C
Cuts at: 1132 3443 4625 5003 1132
Size: 2311 1182 378 3774
Fragments arranged by size:
3774 2311 1182 378
SspI AAT'ATT
Cuts at: 5 52 5569 5
Size: 47 5517 2081
Fragments arranged by size:
5517 2081 47
StuI AGG'CCT
Cuts at: 1517 4142 1517
Size: 2625 5020
StyI C'CwwG_G
Cuts at: 513 1065 2022 4599 4765 5084 513
Size: 552 957 2577 166 319 3074
Fragments arranged by size:
3074 2577 957 552 319 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3179 3545 5672 5689 5842 6027 6472
Size: 1255 1599 366 2127 17 153 185 445
Cuts at: 6472 325
Size: 1498
Fragments arranged by size:
2127 1599 1498 1255 445 366 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2826 5891 7603
Size: 274 80 33 51 1443 849 3065 1712
Cuts at: 7603 96
Size: 138
Fragments arranged by size:
3065 1712 1443 849 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4647 4679 7605 7637 4647
Size: 32 2926 32 4655
Fragments arranged by size:
4655 2926 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7645
XbaI T'CTAG_A
Cuts at: 4981 4981
Size: 7645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2865 4232 2865
Size: 1367 6278
XhoI C'TCGA_G
Cuts at: 1943 2603 4268 1943
Size: 660 1665 5320
Fragments arranged by size:
5320 1665 660
XmaI C'CCGG_G
Cuts at: 1870 2058 4743 4970 1870
Size: 188 2685 227 4545
Fragments arranged by size:
4545 2685 227 188
XmnI GAAnn'nnTTC
Cuts at: 5774 5774
Size: 7645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5453 276
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI RsrII SacII SanDI SfoI SrfI SwaI