Restriction map for
pFN21ASDA1753
(Circular) (Six-base) MAPSORT of: pFN21ASDA1753.seq Check: 9873 from: 1 to: 7159
pFN21ASDA1753 7159 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2210 4138 2210
Size: 1928 5231
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2286 4969 278
Size: 53 83 186 936 750 2683 2468
Fragments arranged by size:
2683 2468 936 750 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 2502 4502 1937
Size: 349 216 2000 4594
Fragments arranged by size:
4594 2000 349 216
Acc65I G'GTAC_C
Cuts at: 2379 4480 2379
Size: 2101 5058
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 3365 5222 6606 6874 1632
Size: 872 861 1857 1384 268 1917
Fragments arranged by size:
1917 1857 1384 872 861 268
AflII C'TTAA_G
Cuts at: 820 1017 4331 820
Size: 197 3314 3648
Fragments arranged by size:
3648 3314 197
AflIII A'CryG_T
Cuts at: 1203 2124 6058 7035 1203
Size: 921 3934 977 1327
Fragments arranged by size:
3934 1327 977 921
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 5888 5888
Size: 7159
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2664 2942 3698 3708 4149 1184
Size: 1480 278 756 10 441 4194
Fragments arranged by size:
4194 1480 756 441 278 10
AlwNI CAG_nnn'CTG
Cuts at: 2092 3376 3885 3953 4207 4902 6474 2092
Size: 1284 509 68 254 695 1572 2777
Fragments arranged by size:
2777 1572 1284 695 509 254 68
ApaI G_GGCC'C
Cuts at: 2036 2043 2481 4279 4438 2036
Size: 7 438 1798 159 4757
Fragments arranged by size:
4757 1798 438 159 7
ApaLI G'TGCA_C
Cuts at: 5216 6372 5216
Size: 1156 6003
ApoI r'AATT_y
Cuts at: 1493 2138 3313 4468 4694 4846 6955 1493
Size: 645 1175 1155 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 1155 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5713 160
Size: 872 4681 1606
Fragments arranged by size:
4681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2105 2894 2949 3916 4119 4484
Size: 73 93 69 789 55 967 203 365
Cuts at: 4484 1870
Size: 4545
Fragments arranged by size:
4545 967 789 365 203 93 73 69
55
AvrII C'CTAG_G
Cuts at: 3148 3148
Size: 7159
BamHI G'GATC_C
Cuts at: 4489 4856 4489
Size: 367 6792
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2232 2261 2379 3082
Size: 325 217 627 278 167 29 118 703
Cuts at: 3082 3491 4100 4480 5935 618
Size: 409 609 380 1455 1842
Fragments arranged by size:
1842 1455 703 627 609 409 380 325
278 217 167 118 29
BanII G_rGCy'C
Cuts at: 1391 2036 2043 2481 2762 3095 3188 3357 3692
Size: 645 7 438 281 333 93 169 335
Cuts at: 3692 4200 4279 4392 4438 4478 1391
Size: 508 79 113 46 40 4072
Fragments arranged by size:
4072 645 508 438 335 333 281 169
113 93 79 46 40 7
BbeI G_GCGC'C
Cuts at: 2069 2236 4104 2069
Size: 167 1868 5124
Fragments arranged by size:
5124 1868 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3214 3291 3629 928
Size: 971 1315 77 338 4458
Fragments arranged by size:
4458 1315 971 338 77
BbvCI CC'TCA_GC
Cuts at: 2621 3016 3838 4398 2621
Size: 395 822 560 5382
Fragments arranged by size:
5382 822 560 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2043 2077 3649 3683 5350 5384 1903
Size: 34 106 34 1572 34 1667 34 3678
Fragments arranged by size:
3678 1667 1572 106 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5051 6261 5051
Size: 1210 5949
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2328 2531 2638
Size: 107 122 71 631 605 656 203 107
Cuts at: 2638 2698 2712 3489 3962 4040 4094 4428 5770
Size: 60 14 777 473 78 54 334 1342
Cuts at: 5770 136
Size: 1525
Fragments arranged by size:
1525 1342 777 656 631 605 473 334
203 122 107 107 78 71 60 54
14
BglII A'GATC_T
Cuts at: 2898 7154 2898
Size: 4256 2903
BlpI GC'TnA_GC
Cuts at: 4576 4576
Size: 7159
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2043 2159 2342 2481 3127 3494 3648
Size: 246 7 116 183 139 646 367 154
Cuts at: 3648 3923 4279 4415 4438 5220 6376 1790
Size: 275 356 136 23 782 1156 2573
Fragments arranged by size:
2573 1156 782 646 367 356 275 246
183 154 139 136 116 23 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3479 4890 5848
Size: 416 53 83 399 24 2062 1411 958
Cuts at: 5848 442
Size: 1753
Fragments arranged by size:
2062 1753 1411 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 4252 2443
Size: 1809 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3082 3230 3442 3638 4145 4160 4193 4244
Size: 1843 148 212 196 507 15 33 51
Cuts at: 4244 4247 4430 5819 1239
Size: 3 183 1389 2579
Fragments arranged by size:
2579 1843 1389 507 212 196 183 148
51 33 15 3
Bpu10I CC'TnA_GC
Cuts at: 2085 2621 3016 3838 4194 4398 2085
Size: 536 395 822 356 204 4846
Fragments arranged by size:
4846 822 536 395 356 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 5284 6149 6447 3418
Size: 1866 865 298 4130
Fragments arranged by size:
4130 1866 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2817 5822 882
Size: 617 1318 3005 2219
Fragments arranged by size:
3005 2219 1318 617
BsaAI yAC'GTr
Cuts at: 493 1204 2127 2977 4456 4950 493
Size: 711 923 850 1479 494 2702
Fragments arranged by size:
2702 1479 923 850 711 494
BsaBI GATnn'nnATC
Cuts at: 7153 7153
Size: 7159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2233 2283 2850
Size: 53 83 186 936 533 167 50 567
Cuts at: 2850 4101 4966 5348 5983 275
Size: 1251 865 382 635 1451
Fragments arranged by size:
1451 1251 936 865 635 567 533 382
186 167 83 53 50
BsaWI w'CCGG_w
Cuts at: 5592 5990 6264 6411 6822 5592
Size: 398 274 147 411 5929
Fragments arranged by size:
5929 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 2621 2651 3066
Size: 30 165 30 1074 30 114 30 415
Cuts at: 3066 3096 1178
Size: 30 5241
Fragments arranged by size:
5241 1074 415 165 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2642 3119 3763 4031 4085 4181 1186
Size: 1456 477 644 268 54 96 4164
Fragments arranged by size:
4164 1456 644 477 268 96 54
BseYI C'CCAG_C
Cuts at: 2585 2659 3036 3400 6362 6858 2585
Size: 74 377 364 2962 496 2886
Fragments arranged by size:
2962 2886 496 377 364 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2275 2333 2425 2441 4166 1163
Size: 573 539 58 92 16 1725 4156
Fragments arranged by size:
4156 1725 573 539 92 58 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 4609 5370 6398 1540
Size: 275 186 75 2533 761 1028 2301
Fragments arranged by size:
2533 2301 1028 761 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 3692 4392 5220 5305 6376 3692
Size: 700 828 85 1071 4475
Fragments arranged by size:
4475 1071 828 700 85
BsiWI C'GTAC_G
Cuts at: 4452 4452
Size: 7159
BsmI GAATG_Cn'
Cuts at: 3520 4670 4763 3520
Size: 1150 93 5916
Fragments arranged by size:
5916 1150 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2790 2841 2790
Size: 51 7108
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2043 2159 2342 2481 2762 3095
Size: 399 246 7 116 183 139 281 333
Cuts at: 3095 3127 3188 3357 3494 3648 3692 3923 4200
Size: 32 61 169 137 154 44 231 277
Cuts at: 4200 4279 4392 4415 4438 4478 5220 5305 6376
Size: 79 113 23 23 40 742 85 1071
Cuts at: 6376 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 333 281 277 246
231 183 169 154 139 137 116 113
85 79 61 44 40 32 23 23
7
BspEI T'CCGG_A
Cuts at: 6822 6822
Size: 7159
BspHI T'CATG_A
Cuts at: 5048 6778 5048
Size: 1730 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2210 2367 3012 4138 4514 844
Size: 1366 157 645 1126 376 3489
Fragments arranged by size:
3489 1366 1126 645 376 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2084 2493 4084 4177 5046 1394
Size: 429 261 409 1591 93 869 3507
Fragments arranged by size:
3507 1591 869 429 409 261 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 5654 5828 65
Size: 1183 4406 174 1396
Fragments arranged by size:
4406 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 2748 5803 5990 1700
Size: 110 428 510 3055 187 2869
Fragments arranged by size:
3055 2869 510 428 187 110
BsrGI T'GTAC_A
Cuts at: 96 3054 96
Size: 2958 4201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 2417 5219 6231 2417
Size: 2802 1012 3345
Fragments arranged by size:
3345 2802 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3376 3784 4410 794
Size: 2582 408 626 3543
Fragments arranged by size:
3543 2582 626 408
BstBI TT'CG_AA
Cuts at: 2007 5963 2007
Size: 3956 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7159
BstXI CCAn_nnnn'nTGG
Cuts at: 2585 2585
Size: 7159
BstYI r'GATC_y
Cuts at: 1972 2616 2898 3851 4489 4856 5241 5258 6710
Size: 644 282 953 638 367 385 17 1452
Cuts at: 6710 6796 6808 7154 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 953 644 638 385 367 346
282 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2473 2526 2553 2696 513
Size: 552 1408 53 27 143 4976
Fragments arranged by size:
4976 1408 552 143 53 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6420
Fragments arranged by size:
6420 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 2458 4016 4362 4675 5469 5489 2159
Size: 299 1558 346 313 794 20 3829
Fragments arranged by size:
3829 1558 794 346 313 299 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7124
DraI TTT'AAA
Cuts at: 4463 4463
Size: 7159
DraIII CAC_nnn'GTG
Cuts at: 2221 2916 2221
Size: 695 6464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6166 809
Size: 507 4850 1802
Fragments arranged by size:
4850 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2545 3609
Size: 54 1425 50 275 18 243 472 1064
Cuts at: 3609 4429 4606 5495 7124 8
Size: 820 177 889 1629 43
Fragments arranged by size:
1629 1425 1064 889 820 472 275 243
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2073 4606 1537
Size: 275 261 2533 4090
Fragments arranged by size:
4090 2533 275 261
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 5089 1353
Size: 2095 1641 3423
Fragments arranged by size:
3423 2095 1641
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 2770 4082 5746 6120 6266
Size: 1064 138 648 533 1312 1664 374 146
Cuts at: 6266 387
Size: 1280
Fragments arranged by size:
1664 1312 1280 1064 648 533 374 146
138
EcoICRI GAG'CTC
Cuts at: 3690 4390 3690
Size: 700 6459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 3082 3230 3365 3442 3638 4145
Size: 393 872 578 148 135 77 196 507
Cuts at: 4145 4160 4193 4244 4247 4430 5222 5819 6606
Size: 15 33 51 3 183 792 597 787
Cuts at: 6606 6874 1239
Size: 268 1524
Fragments arranged by size:
1524 872 792 787 597 578 507 393
268 196 183 148 135 77 51 33
15 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2624 2624
Size: 7159
EcoO109I rG'GnC_Cy
Cuts at: 2032 2033 2478 2954 3044 4190 4204 2032
Size: 1 445 476 90 1146 14 4987
Fragments arranged by size:
4987 1146 476 445 90 14 1
EcoRI G'AATT_C
Cuts at: 2138 4468 4846 2138
Size: 2330 378 4451
Fragments arranged by size:
4451 2330 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3459 3459
Size: 7159
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 2069 2236 4104 4235 6306 1678
Size: 391 167 1868 131 2071 2531
Fragments arranged by size:
2531 2071 1868 391 167 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2564 2596 5800
Size: 32 135 32 905 32 55 32 3204
Cuts at: 5800 5832 5874 5906 1373
Size: 32 42 32 2626
Fragments arranged by size:
3204 2626 905 135 55 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4503 4749 669
Size: 988 281 2565 246 3079
Fragments arranged by size:
3079 2565 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3907 748
Size: 3159 4000
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KasI G'GCGC_C
Cuts at: 2065 2232 4100 2065
Size: 167 1868 5124
Fragments arranged by size:
5124 1868 167
KpnI G_GTAC'C
Cuts at: 2383 4484 2383
Size: 2101 5058
MfeI C'AATT_G
Cuts at: 4758 4758
Size: 7159
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3795 4245 6273 6457 1305
Size: 2490 450 2028 184 2007
Fragments arranged by size:
2490 2028 2007 450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2547 10
Size: 54 1425 343 715 4622
Fragments arranged by size:
4622 1425 715 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2811 5117 5476 5635 518
Size: 648 1645 2306 359 159 2042
Fragments arranged by size:
2306 2042 1645 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2698 2943 3020 4129 4575 5252 6400
Size: 624 468 245 77 1109 446 677 1148
Cuts at: 6400 6645 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1109 677 624 468 446 245
245 77
NarI GG'CG_CC
Cuts at: 2066 2233 4101 2066
Size: 167 1868 5124
Fragments arranged by size:
5124 1868 167
NcoI C'CATG_G
Cuts at: 513 1065 2526 513
Size: 552 1461 5146
Fragments arranged by size:
5146 1461 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7159
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NspI r_CATG'y
Cuts at: 1132 1229 2929 3061 3110 3400 4315 4517 6062
Size: 97 1700 132 49 290 915 202 1545
Cuts at: 6062 1132
Size: 2229
Fragments arranged by size:
2229 1700 1545 915 290 202 132 97
49
PasI CC'CwG_GG
Cuts at: 1348 3227 3796 1348
Size: 1879 569 4711
Fragments arranged by size:
4711 1879 569
PciI A'CATG_T
Cuts at: 6058 6058
Size: 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7159
PfoI T'CCnGG_A
Cuts at: 4022 4161 4022
Size: 139 7020
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 5228 5260 6741 6773 2870
Size: 32 2326 32 1481 32 3256
Fragments arranged by size:
3256 2326 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2954 3044 2954
Size: 90 7069
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7159
PsiI TTA'TAA
Cuts at: 4729 4729
Size: 7159
PspOMI G'GGCC_C
Cuts at: 2032 2039 2477 4275 4434 2032
Size: 7 438 1798 159 4757
Fragments arranged by size:
4757 1798 438 159 7
PstI C_TGCA'G
Cuts at: 830 2097 3009 3687 3789 4511 830
Size: 1267 912 678 102 722 3478
Fragments arranged by size:
3478 1267 912 722 678 102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
PvuII CAG'CTG
Cuts at: 2943 3020 2943
Size: 77 7082
SacI G_AGCT'C
Cuts at: 3692 4392 3692
Size: 700 6459
SacII CC_GC'GG
Cuts at: 2699 2699
Size: 7159
SalI G'TCGA_C
Cuts at: 1936 4501 1936
Size: 2565 4594
SbfI CC_TGCA'GG
Cuts at: 4511 4511
Size: 7159
ScaI AGT'ACT
Cuts at: 2861 5407 7119 2861
Size: 2546 1712 2901
Fragments arranged by size:
2901 2546 1712
SexAI A'CCwGG_T
Cuts at: 3288 3288
Size: 7159
SfcI C'TryA_G
Cuts at: 826 1045 2093 2310 3005 3133 3683 3785 4507
Size: 219 1048 217 695 128 550 102 722
Cuts at: 4507 5642 6323 6514 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 722 695 681 550 219
217 191 128 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 2531 2531
Size: 7159
SfoI GGC'GCC
Cuts at: 2067 2234 4102 2067
Size: 167 1868 5124
Fragments arranged by size:
5124 1868 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 2038 3918 4121 4486 1872
Size: 166 1880 203 365 4545
Fragments arranged by size:
4545 1880 365 203 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3433 4331 5263 6164 6426 820
Size: 197 926 1490 898 932 901 262 1553
Fragments arranged by size:
1553 1490 932 926 901 898 262 197
SnaBI TAC'GTA
Cuts at: 493 4456 4950 493
Size: 3963 494 2702
Fragments arranged by size:
3963 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 3110 4517 1132
Size: 1978 1407 3774
Fragments arranged by size:
3774 1978 1407
SspI AAT'ATT
Cuts at: 5 52 5083 5
Size: 47 5031 2081
Fragments arranged by size:
5031 2081 47
StuI AGG'CCT
Cuts at: 1517 2316 2534 1517
Size: 799 218 6142
Fragments arranged by size:
6142 799 218
StyI C'CwwG_G
Cuts at: 513 1065 2526 2710 3148 3717 4598 513
Size: 552 1461 184 438 569 881 3074
Fragments arranged by size:
3074 1461 881 569 552 438 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3050 3666 5186 5203 5356 5541 5986
Size: 1255 1470 616 1520 17 153 185 445
Cuts at: 5986 325
Size: 1498
Fragments arranged by size:
1520 1498 1470 1255 616 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 3054 5405
Size: 274 80 33 51 1443 882 195 2351
Cuts at: 5405 7117 96
Size: 1712 138
Fragments arranged by size:
2351 1712 1443 882 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2621 2653 3066 3098 3731 3763 7119 7151 2621
Size: 32 413 32 633 32 3356 32 2629
Fragments arranged by size:
3356 2629 633 413 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7159
XbaI T'CTAG_A
Cuts at: 4495 4495
Size: 7159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 2036 3916 4119 4484 1870
Size: 166 1880 203 365 4545
Fragments arranged by size:
4545 1880 365 203 166
XmnI GAAnn'nnTTC
Cuts at: 2138 3314 3626 5288 2138
Size: 1176 312 1662 4009
Fragments arranged by size:
4009 1662 1176 312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2284 4967 276
Size: 53 83 186 936 750 2683 2468
Fragments arranged by size:
2683 2468 936 750 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BstZ17I Bsu36I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SanDI SapI SrfI SwaI XcmI