Restriction map for
pFN21ASDA1748
(Circular) (Six-base) MAPSORT of: pFN21ASDA1748.seq Check: 9531 from: 1 to: 6874
pFN21ASDA1748 6874 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2164 2354 2164
Size: 190 6684
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4684 278
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4217 1937
Size: 2280 4594
Acc65I G'GTAC_C
Cuts at: 4145 4195 4145
Size: 50 6824
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 3435 4040 4937 6321 6589 1632
Size: 559 1244 605 897 1384 268 1917
Fragments arranged by size:
1917 1384 1244 897 605 559 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 2126 5773 6750 1203
Size: 923 3647 977 1327
Fragments arranged by size:
3647 1327 977 923
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 3628 5603 3628
Size: 1975 4899
AleI CACnn'nnGTG
Cuts at: 1166 2382 1166
Size: 1216 5658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2339 1184
Size: 1155 5719
AlwNI CAG_nnn'CTG
Cuts at: 2036 2388 2951 3488 4617 6189 2036
Size: 352 563 537 1129 1572 2721
Fragments arranged by size:
2721 1572 1129 563 537 352
ApaLI G'TGCA_C
Cuts at: 4931 6087 4931
Size: 1156 5718
ApoI r'AATT_y
Cuts at: 1493 2408 2693 4183 4409 4561 6670 1493
Size: 915 285 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 915 285 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5428 160
Size: 872 4396 1606
Fragments arranged by size:
4396 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 2244 3694 3751 4199 1870
Size: 73 301 1450 57 448 4545
Fragments arranged by size:
4545 1450 448 301 73 57
BamHI G'GATC_C
Cuts at: 4204 4571 4204
Size: 367 6507
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3371 3564 3577 4145 4195
Size: 325 217 627 1584 193 13 568 50
Cuts at: 4195 5650 618
Size: 1455 1842
Fragments arranged by size:
1842 1584 1455 627 568 325 217 193
50 13
BanII G_rGCy'C
Cuts at: 1391 2287 3222 3606 4193 1391
Size: 896 935 384 587 4072
Fragments arranged by size:
4072 935 896 587 384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2807 4028 928
Size: 971 908 1221 3774
Fragments arranged by size:
3774 1221 971 908
BbvCI CC'TCA_GC
Cuts at: 2250 2894 2250
Size: 644 6230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3341 3375 5065 5099 1903
Size: 34 1404 34 1690 34 3678
Fragments arranged by size:
3678 1690 1404 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4766 5976 4766
Size: 1210 5664
BclI T'GATC_A
Cuts at: 1548 2413 1548
Size: 865 6009
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3539 3859 5485
Size: 107 122 71 631 605 1867 320 1626
Cuts at: 5485 136
Size: 1525
Fragments arranged by size:
1867 1626 1525 631 605 320 122 107
71
BglII A'GATC_T
Cuts at: 6869 6869
Size: 6874
BlpI GC'TnA_GC
Cuts at: 4066 4291 4066
Size: 225 6649
Bme1580I G_kGCm'C
Cuts at: 1790 2166 3329 3374 4935 6091 1790
Size: 376 1163 45 1561 1156 2573
Fragments arranged by size:
2573 1561 1163 1156 376 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6874
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3830 4605 5563
Size: 416 53 83 399 24 2413 775 958
Cuts at: 5563 442
Size: 1753
Fragments arranged by size:
2413 1753 958 775 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 3193 2572
Size: 621 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2748 2942 3118 3289 3638 5534 1239
Size: 1509 194 176 171 349 1896 2579
Fragments arranged by size:
2579 1896 1509 349 194 176 171
Bpu10I CC'TnA_GC
Cuts at: 2250 2894 2970 2981 3489 3863 4060 2250
Size: 644 76 11 508 374 197 5064
Fragments arranged by size:
5064 644 508 374 197 76 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3296 4999 5864 6162 3296
Size: 1703 865 298 4008
Fragments arranged by size:
4008 1703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2077 2779 5537 882
Size: 617 578 702 2758 2219
Fragments arranged by size:
2758 2219 702 617 578
BsaAI yAC'GTr
Cuts at: 493 1204 2127 2644 4171 4665 493
Size: 711 923 517 1527 494 2702
Fragments arranged by size:
2702 1527 923 711 517 494
BsaBI GATnn'nnATC
Cuts at: 6868 6868
Size: 6874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3826 4681 5063 5698
Size: 53 83 186 936 2293 855 382 635
Cuts at: 5698 275
Size: 1451
Fragments arranged by size:
2293 1451 936 855 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5307 5705 5979 6126 6537 5307
Size: 398 274 147 411 5644
Fragments arranged by size:
5644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3675 3705 1178
Size: 30 165 30 2272 30 4347
Fragments arranged by size:
4347 2272 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2911 2955 3292 3769 4131 1186
Size: 1081 644 44 337 477 362 3929
Fragments arranged by size:
3929 1081 644 477 362 337 44
BseYI C'CCAG_C
Cuts at: 2054 3203 3221 3340 3543 3829 6077 6573 2054
Size: 1149 18 119 203 286 2248 496 2355
Fragments arranged by size:
2355 2248 1149 496 286 203 119 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3497 1163
Size: 573 1761 4540
Fragments arranged by size:
4540 1761 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3089 3923 4324 5085 6113 1540
Size: 275 186 1088 834 401 761 1028 2301
Fragments arranged by size:
2301 1088 1028 834 761 401 275 186
BsiHKAI G_wGCw'C
Cuts at: 2287 4935 5020 6091 2287
Size: 2648 85 1071 3070
Fragments arranged by size:
3070 2648 1071 85
BsmI GAATG_Cn'
Cuts at: 3127 3732 4385 4478 3127
Size: 605 653 93 5523
Fragments arranged by size:
5523 653 605 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2287 3222 3329 3374 3606 4193
Size: 399 376 121 935 107 45 232 587
Cuts at: 4193 4935 5020 6091 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 935 742 587 399 376 232
121 107 85 45
BspEI T'CCGG_A
Cuts at: 6537 6537
Size: 6874
BspHI T'CATG_A
Cuts at: 4763 6493 4763
Size: 1730 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2164 2354 4229 844
Size: 1320 190 1875 3489
Fragments arranged by size:
3489 1875 1320 190
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 4761 1394
Size: 429 1390 1548 3507
Fragments arranged by size:
3507 1548 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3421 5369 5543 65
Size: 1183 2173 1948 174 1396
Fragments arranged by size:
2173 1948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5518 5705 1700
Size: 110 3708 187 2869
Fragments arranged by size:
3708 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3075 96
Size: 2979 3895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6874
BssSI C'ACGA_G
Cuts at: 4108 4934 5946 4108
Size: 826 1012 5036
Fragments arranged by size:
5036 1012 826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6874
BstBI TT'CG_AA
Cuts at: 2007 5678 2007
Size: 3671 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6874
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2779 2224
Size: 555 6319
BstYI r'GATC_y
Cuts at: 1972 4204 4571 4956 4973 6425 6511 6523 6869
Size: 2232 367 385 17 1452 86 12 346
Cuts at: 6869 1972
Size: 1977
Fragments arranged by size:
2232 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6135
Fragments arranged by size:
6135 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 2377 2389 2419 4390 5184 5204 2166
Size: 211 12 30 1971 794 20 3836
Fragments arranged by size:
3836 1971 794 211 30 20 12
ClaI AT'CG_AT
Cuts at: 1554 3923 1554
Size: 2369 4505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6839
DraI TTT'AAA
Cuts at: 4178 4178
Size: 6874
DraIII CAC_nnn'GTG
Cuts at: 3643 3643
Size: 6874
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5881 809
Size: 507 4565 1802
Fragments arranged by size:
4565 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2214 2655
Size: 54 1425 50 275 18 369 15 441
Cuts at: 2655 3045 3086 3399 4321 5210 6839 8
Size: 390 41 313 922 889 1629 43
Fragments arranged by size:
1629 1425 922 889 441 390 369 313
275 54 50 43 41 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3086 4321 1537
Size: 275 1274 1235 4090
Fragments arranged by size:
4090 1274 1235 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 4804 1353
Size: 764 2687 3423
Fragments arranged by size:
3423 2687 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5461 5835 5981 387
Size: 1064 138 3872 374 146 1280
Fragments arranged by size:
3872 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2285 2285
Size: 6874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2748 2942 3118 3289 3435 3638
Size: 393 559 557 194 176 171 146 203
Cuts at: 3638 4040 4937 5534 6321 6589 1239
Size: 402 897 597 787 268 1524
Fragments arranged by size:
1524 897 787 597 559 557 402 393
268 203 194 176 171 146
EcoNI CCTnn'n_nnAGG
Cuts at: 3891 4080 3891
Size: 189 6685
EcoO109I rG'GnC_Cy
Cuts at: 3485 3882 3485
Size: 397 6477
EcoRI G'AATT_C
Cuts at: 4183 4561 4183
Size: 378 6496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3444 3444
Size: 6874
FspI TGC'GCA
Cuts at: 2327 5380 2327
Size: 3053 3821
FspAI rTGC'GCAy
Cuts at: 2327 2327
Size: 6874
HaeII r_GCGC'y
Cuts at: 1678 3141 6021 1678
Size: 1463 2880 2531
Fragments arranged by size:
2880 2531 1463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 2924 2956 3181
Size: 32 135 32 655 32 665 32 225
Cuts at: 3181 3213 3269 3301 3455 3487 3614 3646 5515
Size: 32 56 32 154 32 127 32 1869
Cuts at: 5515 5547 5589 5621 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1869 665 655 225 154 135 127
56 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3741 4218 4464 669
Size: 988 281 1803 477 246 3079
Fragments arranged by size:
3079 1803 988 477 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6874
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KpnI G_GTAC'C
Cuts at: 4149 4199 4149
Size: 50 6824
MfeI C'AATT_G
Cuts at: 2805 3180 4473 2805
Size: 375 1293 5206
Fragments arranged by size:
5206 1293 375
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4065 5988 6172 1305
Size: 2760 1923 184 2007
Fragments arranged by size:
2760 2007 1923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2216 2657 3047 3401
Size: 54 1425 343 369 15 441 390 354
Cuts at: 3401 10
Size: 3483
Fragments arranged by size:
3483 1425 441 390 369 354 343 54
15
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2321 2382 4832 5191 5350 518
Size: 648 1056 99 61 2450 359 159 2042
Fragments arranged by size:
2450 2042 1056 648 359 159 99 61
MspA1I CmG'CkG
Cuts at: 1606 3116 3833 4018 4290 4967 6115 6360 1606
Size: 1510 717 185 272 677 1148 245 2120
Fragments arranged by size:
2120 1510 1148 717 677 272 245 185
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6322
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6874
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6874
NotI GC'GGCC_GC
Cuts at: 4321 4321
Size: 6874
NspI r_CATG'y
Cuts at: 1132 1229 4232 5777 1132
Size: 97 3003 1545 2229
Fragments arranged by size:
3003 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2231 2427 3322 1348
Size: 883 196 895 4900
Fragments arranged by size:
4900 895 883 196
PciI A'CATG_T
Cuts at: 5773 5773
Size: 6874
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2503 3643 1108
Size: 1395 1140 4339
Fragments arranged by size:
4339 1395 1140
PfoI T'CCnGG_A
Cuts at: 2131 3654 2131
Size: 1523 5351
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PmlI CAC'GTG
Cuts at: 2644 2644
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 3261 4943 4975 6456 6488 3229
Size: 32 1682 32 1481 32 3615
Fragments arranged by size:
3615 1682 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3882 3882
Size: 6874
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 4444 4444
Size: 6874
PspXI vC'TCGA_Gb
Cuts at: 2244 2244
Size: 6874
PstI C_TGCA'G
Cuts at: 830 2080 2161 2384 2759 2947 4226 830
Size: 1250 81 223 375 188 1279 3478
Fragments arranged by size:
3478 1279 1250 375 223 188 81
PvuI CG_AT'CG
Cuts at: 2001 3923 2001
Size: 1922 4952
PvuII CAG'CTG
Cuts at: 3116 3833 4018 3116
Size: 717 185 5972
Fragments arranged by size:
5972 717 185
SacI G_AGCT'C
Cuts at: 2287 2287
Size: 6874
SalI G'TCGA_C
Cuts at: 1936 4216 1936
Size: 2280 4594
SanDI GG'GwC_CC
Cuts at: 3882 3882
Size: 6874
SbfI CC_TGCA'GG
Cuts at: 4226 4226
Size: 6874
ScaI AGT'ACT
Cuts at: 5122 6834 5122
Size: 1712 5162
SexAI A'CCwGG_T
Cuts at: 3952 3952
Size: 6874
SfcI C'TryA_G
Cuts at: 826 1045 2076 2157 2380 2619 2755 2943 4030
Size: 219 1031 81 223 239 136 188 1087
Cuts at: 4030 4222 5357 6038 6229 826
Size: 192 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 1031 681 239 223 219
192 191 188 136 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 3696 4201 1872
Size: 1824 505 4545
Fragments arranged by size:
4545 1824 505
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2244 3311 4978 5879 6141 820
Size: 197 926 301 1067 1667 901 262 1553
Fragments arranged by size:
1667 1553 1067 926 901 301 262 197
SnaBI TAC'GTA
Cuts at: 493 4171 4665 493
Size: 3678 494 2702
Fragments arranged by size:
3678 2702 494
SpeI A'CTAG_T
Cuts at: 152 3770 152
Size: 3618 3256
SphI G_CATG'C
Cuts at: 1132 4232 1132
Size: 3100 3774
SspI AAT'ATT
Cuts at: 5 52 4798 5
Size: 47 4746 2081
Fragments arranged by size:
4746 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6874
StyI C'CwwG_G
Cuts at: 513 1065 3048 4313 513
Size: 552 1983 1265 3074
Fragments arranged by size:
3074 1983 1265 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 2495 2785 3582 4015 4901 4918
Size: 1255 839 76 290 797 433 886 17
Cuts at: 4918 5071 5256 5701 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 886 839 797 445 433 290
185 153 76 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3075 4012 5120
Size: 274 80 33 51 1443 1098 937 1108
Cuts at: 5120 6832 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1108 1098 937 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2628 2660 3675 3707 6834 6866 2628
Size: 32 1015 32 3127 32 2636
Fragments arranged by size:
3127 2636 1015 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6874
XbaI T'CTAG_A
Cuts at: 4210 4210
Size: 6874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 2209 3413 2092
Size: 117 1204 5553
Fragments arranged by size:
5553 1204 117
XhoI C'TCGA_G
Cuts at: 1943 2244 1943
Size: 301 6573
XmaI C'CCGG_G
Cuts at: 1870 3694 4199 1870
Size: 1824 505 4545
Fragments arranged by size:
4545 1824 505
XmnI GAAnn'nnTTC
Cuts at: 2714 5003 2714
Size: 2289 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4682 276
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI