Restriction map for
pFN21ASDA1737
(Circular) (Six-base) MAPSORT of: pFN21ASDA1737.seq Check: 3012 from: 1 to: 5926
pFN21ASDA1737 5926 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2720 2720
Size: 5926
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2735 3989 5373 5641 1632
Size: 1103 1254 1384 268 1917
Fragments arranged by size:
1917 1384 1254 1103 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 2451 1166
Size: 1285 4641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaI G_GGCC'C
Cuts at: 3127 3127
Size: 5926
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2494 3235 3461 3613 5722 1493
Size: 1001 741 226 152 2109 1697
Fragments arranged by size:
2109 1697 1001 741 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 3251 1870
Size: 73 1308 4545
Fragments arranged by size:
4545 1308 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3247 4702 618
Size: 325 217 627 1460 1455 1842
Fragments arranged by size:
1842 1460 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2168 2183 3127 3245 1391
Size: 663 114 15 944 118 4072
Fragments arranged by size:
4072 944 663 118 114 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 2244 2607 928
Size: 971 166 179 363 4247
Fragments arranged by size:
4247 971 363 179 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2770 3818 5028 2770
Size: 1048 1210 3668
Fragments arranged by size:
3668 1210 1048
BclI T'GATC_A
Cuts at: 1548 3205 1548
Size: 1657 4269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3131 4537 136
Size: 107 122 71 631 605 1459 1406 1525
Fragments arranged by size:
1525 1459 1406 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 2213 3343 2213
Size: 1130 4796
Bme1580I G_kGCm'C
Cuts at: 1790 2718 3127 3987 5143 1790
Size: 928 409 860 1156 2573
Fragments arranged by size:
2573 1156 928 860 409
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2781 3657 4615
Size: 416 53 83 399 24 1364 876 958
Cuts at: 4615 442
Size: 1753
Fragments arranged by size:
1753 1364 958 876 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2877 2875
Size: 2 5924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2248 2333 2455 3032 4586 1239
Size: 1009 85 122 577 1554 2579
Fragments arranged by size:
2579 1554 1009 577 122 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2773 3223 3717 493
Size: 711 1569 450 494 2702
Fragments arranged by size:
2702 1569 711 494 450
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2663 3733 4115 4750
Size: 53 83 186 936 1130 1070 382 635
Cuts at: 4750 275
Size: 1451
Fragments arranged by size:
1451 1130 1070 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2275 2305 1178
Size: 30 165 30 872 30 4799
Fragments arranged by size:
4799 872 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5926
BseYI C'CCAG_C
Cuts at: 2343 2675 3093 3211 5129 5625 2343
Size: 332 418 118 1918 496 2644
Fragments arranged by size:
2644 1918 496 418 332 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 1163
Size: 573 858 4495
Fragments arranged by size:
4495 858 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2168 2183 2916 3987 4072 5143 2168
Size: 15 733 1071 85 1071 2951
Fragments arranged by size:
2951 1071 1071 733 85 15
BsmI GAATG_Cn'
Cuts at: 2278 3197 3437 3530 2278
Size: 919 240 93 4674
Fragments arranged by size:
4674 919 240 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2168 2183 2718 2916 3127 3245
Size: 399 264 114 15 535 198 211 118
Cuts at: 3245 3987 4072 5143 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 535 399 264 211 198
118 114 85 15
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 2304 3815 5545 2304
Size: 1511 1730 2685
Fragments arranged by size:
2685 1730 1511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2720 3281 844
Size: 1876 561 3489
Fragments arranged by size:
3489 1876 561
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2755 4570 4757 1700
Size: 110 945 1815 187 2869
Fragments arranged by size:
2869 1815 945 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2273 2713 794
Size: 1479 440 4007
Fragments arranged by size:
4007 1479 440
BstBI TT'CG_AA
Cuts at: 2007 4730 2007
Size: 2723 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921
Size: 1284 367 385 17 1452 86 12 346
Cuts at: 5921 1972
Size: 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2221 3129 2221
Size: 908 5018
BtgI C'CryG_G
Cuts at: 513 1065 2022 2295 2842 513
Size: 552 957 273 547 3597
Fragments arranged by size:
3597 957 552 547 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3442 4236 4256 3190
Size: 252 794 20 4860
Fragments arranged by size:
4860 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3373 4262 5891
Size: 54 1425 50 275 18 1543 889 1629
Cuts at: 5891 8
Size: 43
Fragments arranged by size:
1629 1543 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2754 3856 1353
Size: 1401 1102 3423
Fragments arranged by size:
3423 1401 1102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2166 2181 2166
Size: 15 5911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2248 2333 2455 2735 3032 3989 4586
Size: 393 616 85 122 280 297 957 597
Cuts at: 4586 5373 5641 1239
Size: 787 268 1524
Fragments arranged by size:
1524 957 787 616 597 393 297 280
268 122 85
EcoO109I rG'GnC_Cy
Cuts at: 2533 3123 3124 2533
Size: 590 1 5335
Fragments arranged by size:
5335 590 1
EcoRI G'AATT_C
Cuts at: 2494 3235 3613 2494
Size: 741 378 4807
Fragments arranged by size:
4807 741 378
EcoRV GAT'ATC
Cuts at: 2935 2935
Size: 5926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 5926
FspI TGC'GCA
Cuts at: 2824 4432 2824
Size: 1608 4318
HaeII r_GCGC'y
Cuts at: 1678 2220 2682 2971 5073 1678
Size: 542 462 289 2102 2531
Fragments arranged by size:
2531 2102 542 462 289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2150 2161 2182 2447
Size: 32 135 32 557 21 11 21 265
Cuts at: 2447 2479 4567 4599 4641 4673 1373
Size: 32 2088 32 42 32 2626
Fragments arranged by size:
2626 2088 557 265 135 42 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 5040 5224 1305
Size: 1185 2550 184 2007
Fragments arranged by size:
2550 2007 1185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2451 2899 3884 4243 4402 518
Size: 648 924 361 448 985 359 159 2042
Fragments arranged by size:
2042 985 924 648 448 361 359 159
MspA1I CmG'CkG
Cuts at: 1606 2950 3097 3342 4019 5167 5412 1606
Size: 1344 147 245 677 1148 245 2120
Fragments arranged by size:
2120 1344 1148 677 245 245 147
NcoI C'CATG_G
Cuts at: 513 1065 2022 2295 2842 513
Size: 552 957 273 547 3597
Fragments arranged by size:
3597 957 552 547 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2212 3284 4829 1132
Size: 97 983 1072 1545 2229
Fragments arranged by size:
2229 1545 1072 983 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PfoI T'CCnGG_A
Cuts at: 2471 2471
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PmlI CAC'GTG
Cuts at: 2773 2773
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 3123 3123
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2188 2709 2911 3150 3278 830
Size: 1358 521 202 239 128 3478
Fragments arranged by size:
3478 1358 521 239 202 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 3097 3097
Size: 5926
SacI G_AGCT'C
Cuts at: 2168 2183 2168
Size: 15 5911
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2184 2705 2907 3146 3274 4409 5090
Size: 219 1139 521 202 239 128 1135 681
Cuts at: 5090 5281 826
Size: 191 1471
Fragments arranged by size:
1471 1139 1135 681 521 239 219 202
191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3223 3717 493
Size: 2730 494 2702
Fragments arranged by size:
2730 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2022 2295 2542 2631 2842 3030 3365
Size: 552 957 273 247 89 211 188 335
Cuts at: 3365 513
Size: 3074
Fragments arranged by size:
3074 957 552 335 273 247 211 188
89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2689 3225 3953 3970 4123 4308 4753
Size: 1255 1109 536 728 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 1109 728 536 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2588 4172 5884
Size: 274 80 33 51 1443 611 1584 1712
Cuts at: 5884 96
Size: 138
Fragments arranged by size:
1712 1584 1443 611 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 2770
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 2276 4055 2276
Size: 1779 4147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI