Restriction map for
pFN21ASDA1735
(Circular) (Six-base) MAPSORT of: pFN21ASDA1735.seq Check: 8918 from: 1 to: 6145
pFN21ASDA1735 6145 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 2355 2531 2675 2883 2953 4208 5592
Size: 525 198 176 144 208 70 1255 1384
Cuts at: 5592 5860 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1255 525 268 208 198 176
144 70
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 3213 3309 4976 3213
Size: 96 1667 4382
Fragments arranged by size:
4382 1667 96
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AlwNI CAG_nnn'CTG
Cuts at: 3186 3888 5460 3186
Size: 702 1572 3871
Fragments arranged by size:
3871 1572 702
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 2384 3470 1870
Size: 73 238 203 1086 4545
Fragments arranged by size:
4545 1086 238 203 73
BamHI G'GATC_C
Cuts at: 2324 3331 3475 3842 2324
Size: 1007 144 367 4627
Fragments arranged by size:
4627 1007 367 144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3466 4921 618
Size: 325 217 627 1679 1455 1842
Fragments arranged by size:
1842 1679 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2204 3167 3464 1391
Size: 813 963 297 4072
Fragments arranged by size:
4072 963 813 297
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3429 928
Size: 971 1530 3644
Fragments arranged by size:
3644 1530 971
BbvCI CC'TCA_GC
Cuts at: 2985 2985
Size: 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2663 2697 2918 2952 4336 4370 1903
Size: 34 726 34 221 34 1384 34 3678
Fragments arranged by size:
3678 1384 726 221 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 4206 5362 1790
Size: 2416 1156 2573
Fragments arranged by size:
2573 2416 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2052 2092 3876
Size: 416 53 83 399 24 635 40 1784
Cuts at: 3876 4834 442
Size: 958 1753
Fragments arranged by size:
1784 1753 958 635 416 399 83 53
40 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2541 2963 2541
Size: 422 5723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 4805 1239
Size: 998 2568 2579
Fragments arranged by size:
2579 2568 998
Bpu10I CC'TnA_GC
Cuts at: 2985 2985
Size: 6145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3087 4270 5135 5433 3087
Size: 1183 865 298 3799
Fragments arranged by size:
3799 1183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 4808 882
Size: 617 714 2595 2219
Fragments arranged by size:
2595 2219 714 617
BsaAI yAC'GTr
Cuts at: 493 1204 3442 3936 493
Size: 711 2238 494 2702
Fragments arranged by size:
2702 2238 711 494
BsaBI GATnn'nnATC
Cuts at: 2402 6139 2402
Size: 3737 2408
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3952 4334 4969 275
Size: 53 83 186 936 2419 382 635 1451
Fragments arranged by size:
2419 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2724 3213 3309 4578 4976 5250 5397 5808 2724
Size: 489 96 1269 398 274 147 411 3061
Fragments arranged by size:
3061 1269 489 411 398 274 147 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2155 2185 3251 3281 1178
Size: 30 165 30 752 30 1066 30 4042
Fragments arranged by size:
4042 1066 752 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 3272 3371 1186
Size: 1261 825 99 3960
Fragments arranged by size:
3960 1261 825 99
BseYI C'CCAG_C
Cuts at: 2668 5348 5844 2668
Size: 2680 496 2969
Fragments arranged by size:
2969 2680 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 1163
Size: 573 843 4729
Fragments arranged by size:
4729 843 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3167 3201 4206 4291 5362 3167
Size: 34 1005 85 1071 3950
Fragments arranged by size:
3950 1071 1005 85 34
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
BsmBI CGTCTCn'nnnn_
Cuts at: 3047 3047
Size: 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 3167 3201 3464 4206 4291 5362
Size: 399 414 963 34 263 742 85 1071
Cuts at: 5362 1391
Size: 2174
Fragments arranged by size:
2174 1071 963 742 414 399 263 85
34
BspEI T'CCGG_A
Cuts at: 2724 5808 2724
Size: 3084 3061
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2934 3500 844
Size: 2090 566 3489
Fragments arranged by size:
3489 2090 566
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2866 4640 4814 65
Size: 1183 1618 1774 174 1396
Fragments arranged by size:
1774 1618 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2640 3213 3309 4789 4976 1700
Size: 110 830 573 96 1480 187 2869
Fragments arranged by size:
2869 1480 830 573 187 110 96
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 2007 4949 2007
Size: 2942 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2324 2611 2758 3331 3475 3842 4227 4244
Size: 352 287 147 573 144 367 385 17
Cuts at: 4244 5696 5782 5794 6140 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 573 385 367 352 346 287
147 144 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3427 513
Size: 552 957 1405 3231
Fragments arranged by size:
3231 1405 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 3661 4455 4475 3661
Size: 794 20 5331
Fragments arranged by size:
5331 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3280 3449 3280
Size: 169 5976
DraIII CAC_nnn'GTG
Cuts at: 2755 2755
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2192 2974 3592
Size: 54 1425 50 275 18 362 782 618
Cuts at: 3592 4481 6110 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 782 618 362 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 4075 1353
Size: 747 1975 3423
Fragments arranged by size:
3423 1975 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3165 3165
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2237 2355 2531 2675 2883 2953
Size: 393 525 80 118 176 144 208 70
Cuts at: 2953 4208 4805 5592 5860 1239
Size: 1255 597 787 268 1524
Fragments arranged by size:
1524 1255 787 597 525 393 268 208
176 144 118 80 70
EcoO109I rG'GnC_Cy
Cuts at: 2352 2352
Size: 6145
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
EcoRV GAT'ATC
Cuts at: 2295 2295
Size: 6145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 6145
FspI TGC'GCA
Cuts at: 2960 4651 2960
Size: 1691 4454
HaeII r_GCGC'y
Cuts at: 1678 3040 5292 1678
Size: 1362 2252 2531
Fragments arranged by size:
2531 2252 1362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 4786 4818 4860
Size: 32 135 32 583 32 2599 32 42
Cuts at: 4860 4892 1373
Size: 32 2626
Fragments arranged by size:
2626 2599 583 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 2900 3194 3234 5259 5443 1305
Size: 918 677 294 40 2025 184 2007
Fragments arranged by size:
2025 2007 918 677 294 184 40
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2976 10
Size: 54 1425 343 1144 3179
Fragments arranged by size:
3179 1425 1144 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2784 3372 4103 4462 4621 518
Size: 648 1618 588 731 359 159 2042
Fragments arranged by size:
2042 1618 731 648 588 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2239 3561 4238 5386 5631 1606
Size: 529 104 1322 677 1148 245 2120
Fragments arranged by size:
2120 1322 1148 677 529 245 104
NcoI C'CATG_G
Cuts at: 513 1065 2022 3427 513
Size: 552 957 1405 3231
Fragments arranged by size:
3231 1405 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3047 3503 5048 1132
Size: 97 1818 456 1545 2229
Fragments arranged by size:
2229 1818 1545 456 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2608 2755 1108
Size: 1500 147 4498
Fragments arranged by size:
4498 1500 147
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2036 1613
Size: 423 5722
PsiI TTA'TAA
Cuts at: 2093 2402 3715 2093
Size: 309 1313 4523
Fragments arranged by size:
4523 1313 309
PstI C_TGCA'G
Cuts at: 830 2619 2931 3497 830
Size: 1789 312 566 3478
Fragments arranged by size:
3478 1789 566 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2239 2239
Size: 6145
SacI G_AGCT'C
Cuts at: 3167 3167
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2371 2751 2371
Size: 380 5765
SfcI C'TryA_G
Cuts at: 826 1045 2303 2615 2927 3493 4628 5309 5500
Size: 219 1258 312 312 566 1135 681 191
Cuts at: 5500 826
Size: 1471
Fragments arranged by size:
1471 1258 1135 681 566 312 312 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2181 3102 4249 5150 5412 820
Size: 197 926 238 921 1147 901 262 1553
Fragments arranged by size:
1553 1147 926 921 901 262 238 197
SnaBI TAC'GTA
Cuts at: 493 3442 3936 493
Size: 2949 494 2702
Fragments arranged by size:
2949 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 2258 4069 5
Size: 47 2206 1811 2081
Fragments arranged by size:
2206 2081 1811 47
StuI AGG'CCT
Cuts at: 1517 2309 1517
Size: 792 5353
StyI C'CwwG_G
Cuts at: 513 1065 2022 2355 3427 3584 513
Size: 552 957 333 1072 157 3074
Fragments arranged by size:
3074 1072 957 552 333 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2071 3109 3403 4172 4189 4342 4527
Size: 1255 491 1038 294 769 17 153 185
Cuts at: 4527 4972 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1038 769 491 445 294 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3188 4391 6103
Size: 274 80 33 51 1443 1211 1203 1712
Cuts at: 6103 96
Size: 138
Fragments arranged by size:
1712 1443 1211 1203 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6105 6137 6105
Size: 32 6113
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 2761 3481 2761
Size: 720 5425
XhoI C'TCGA_G
Cuts at: 1943 2181 1943
Size: 238 5907
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI