Restriction map for
pFN21ASDA1733
(Circular) (Six-base) MAPSORT of: pFN21ASDA1733.seq Check: 4726 from: 1 to: 6181
pFN21ASDA1733 6181 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3197 3524 1937
Size: 1260 327 4594
Fragments arranged by size:
4594 1260 327
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 4244 5628 5896 1632
Size: 887 1725 1384 268 1917
Fragments arranged by size:
1917 1725 1384 887 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 2427 5080 6057 1203
Size: 1224 2653 977 1327
Fragments arranged by size:
2653 1327 1224 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 3063 1166
Size: 1897 4284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6181
AlwNI CAG_nnn'CTG
Cuts at: 2572 2690 3001 3924 5496 2572
Size: 118 311 923 1572 3257
Fragments arranged by size:
3257 1572 923 311 118
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 3490 3716 3868 5977 1493
Size: 1997 226 152 2109 1697
Fragments arranged by size:
2109 1997 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2159 4735 160
Size: 872 1127 2576 1606
Fragments arranged by size:
2576 1606 1127 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2944 3061 3506 1870
Size: 73 1001 117 445 4545
Fragments arranged by size:
4545 1001 445 117 73
BamHI G'GATC_C
Cuts at: 2635 3511 3878 2635
Size: 876 367 4938
Fragments arranged by size:
4938 876 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2693 3398 3502 4957
Size: 325 217 627 537 369 705 104 1455
Cuts at: 4957 618
Size: 1842
Fragments arranged by size:
1842 1455 705 627 537 369 325 217
104
BanII G_rGCy'C
Cuts at: 1391 2122 2579 2970 3500 1391
Size: 731 457 391 530 4072
Fragments arranged by size:
4072 731 530 457 391
BbeI G_GCGC'C
Cuts at: 2697 2697
Size: 6181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3186 3237 928
Size: 971 1287 51 3872
Fragments arranged by size:
3872 1287 971 51
BbvCI CC'TCA_GC
Cuts at: 2274 3383 2274
Size: 1109 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4792 136
Size: 107 122 71 631 605 3120 1525
Fragments arranged by size:
3120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 3419 3598 3419
Size: 179 6002
Bme1580I G_kGCm'C
Cuts at: 1790 4242 5398 1790
Size: 2452 1156 2573
Fragments arranged by size:
2573 2452 1156
BmgBI CAC'GTC
Cuts at: 1318 2430 1318
Size: 1112 5069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3912 4870 442
Size: 416 53 83 399 24 2495 958 1753
Fragments arranged by size:
2495 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2563 3398 4841 1239
Size: 1324 835 1443 2579
Fragments arranged by size:
2579 1443 1324 835
Bpu10I CC'TnA_GC
Cuts at: 2274 2573 3292 3383 2274
Size: 299 719 91 5072
Fragments arranged by size:
5072 719 299 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2106 4306 5171 5469 2106
Size: 2200 865 298 2818
Fragments arranged by size:
2818 2200 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3478 3972 493
Size: 711 2274 494 2702
Fragments arranged by size:
2702 2274 711 494
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2694 3988 4370 5005
Size: 53 83 186 936 1161 1294 382 635
Cuts at: 5005 275
Size: 1451
Fragments arranged by size:
1451 1294 1161 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2202 4614 5012 5286 5433 5844 2202
Size: 2412 398 274 147 411 2539
Fragments arranged by size:
2539 2412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2897 2927 3242 3272 3302
Size: 30 165 30 1494 30 315 30 30
Cuts at: 3302 3332 1178
Size: 30 4027
Fragments arranged by size:
4027 1494 315 165 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 3224 3233 3236 3383 1186
Size: 1319 719 9 3 147 3984
Fragments arranged by size:
3984 1319 719 147 9 3
BseYI C'CCAG_C
Cuts at: 2838 5384 5880 2838
Size: 2546 496 3139
Fragments arranged by size:
3139 2546 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5608
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2122 2579 2658 2970 4242 4327 5398 2122
Size: 457 79 312 1272 85 1071 2905
Fragments arranged by size:
2905 1272 1071 457 312 85 79
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2579 2658 2970 3500 4242 4327
Size: 399 332 457 79 312 530 742 85
Cuts at: 4327 5398 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 530 457 399 332 312
85 79
BspEI T'CCGG_A
Cuts at: 2202 5844 2202
Size: 3642 2539
BspHI T'CATG_A
Cuts at: 2757 3163 4070 5800 2757
Size: 406 907 1730 3138
Fragments arranged by size:
3138 1730 907 406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3536 844
Size: 2692 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3373 4068 1394
Size: 429 1550 695 3507
Fragments arranged by size:
3507 1550 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 2921 4825 5012 1700
Size: 110 380 731 1904 187 2869
Fragments arranged by size:
2869 1904 731 380 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2881 794
Size: 2087 4094
BstBI TT'CG_AA
Cuts at: 2007 4985 2007
Size: 2978 3203
BstEII G'GTnAC_C
Cuts at: 1184 3073 1184
Size: 1889 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2819 3050 3262 2161
Size: 658 231 212 5080
Fragments arranged by size:
5080 658 231 212
BstYI r'GATC_y
Cuts at: 1972 2250 2635 2686 2868 3511 3878 4263 4280
Size: 278 385 51 182 643 367 385 17
Cuts at: 4280 5732 5818 5830 6176 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 643 385 385 367 346 278
182 86 51 17 12
BstZ17I GTA'TAC
Cuts at: 3198 3198
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2022 2772 2896 513
Size: 552 957 750 124 3798
Fragments arranged by size:
3798 957 750 552 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2312 487
Size: 683 56 1086 4356
Fragments arranged by size:
4356 1086 683 56
BtsI GCAGTG_nn'
Cuts at: 3697 4491 4511 3697
Size: 794 20 5367
Fragments arranged by size:
5367 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 5188 809
Size: 507 999 2873 1802
Fragments arranged by size:
2873 1802 999 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 2899 2982
Size: 54 1425 50 275 18 991 78 83
Cuts at: 2982 3264 3628 4517 6146 8
Size: 282 364 889 1629 43
Fragments arranged by size:
1629 1425 991 889 364 282 275 83
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2538 2810 3190 3201 3210 4111 1353
Size: 1116 69 272 380 11 9 901 3423
Fragments arranged by size:
3423 1116 901 380 272 69 11 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2120 2577 2968 2120
Size: 457 391 5333
Fragments arranged by size:
5333 457 391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2563 3398 4244 4841 5628 5896
Size: 393 887 44 835 846 597 787 268
Cuts at: 5896 1239
Size: 1524
Fragments arranged by size:
1524 887 846 835 787 597 393 268
44
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 6181
EcoO109I rG'GnC_Cy
Cuts at: 3022 3086 3315 3022
Size: 64 229 5888
Fragments arranged by size:
5888 229 64
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 6181
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 2697 5328 1678
Size: 1019 2631 2531
Fragments arranged by size:
2631 2531 1019
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2072 2077 2104 2627
Size: 32 135 32 473 27 5 27 523
Cuts at: 2627 2659 3227 3259 4822 4854 4896 4928 1373
Size: 32 568 32 1563 32 42 32 2626
Fragments arranged by size:
2626 1563 568 523 473 135 42 32
32 32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3525 3771 669
Size: 988 281 1587 246 3079
Fragments arranged by size:
3079 1587 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KasI G'GCGC_C
Cuts at: 2693 2693
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2683 5295 5479 1305
Size: 1378 2612 184 2007
Fragments arranged by size:
2612 2007 1378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2823 2984 3266 10
Size: 54 1425 343 991 161 282 2925
Fragments arranged by size:
2925 1425 991 343 282 161 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2990 3063 4139 4498 4657 518
Size: 648 993 831 73 1076 359 159 2042
Fragments arranged by size:
2042 1076 993 831 648 359 159 73
MspA1I CmG'CkG
Cuts at: 1606 2273 2587 2881 3019 3597 4274 5422 5667
Size: 667 314 294 138 578 677 1148 245
Cuts at: 5667 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 667 578 314 294 245
138
NarI GG'CG_CC
Cuts at: 2694 2694
Size: 6181
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4672
Fragments arranged by size:
4672 957 552
NdeI CA'TA_TG
Cuts at: 387 2647 387
Size: 2260 3921
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3539 5084 1132
Size: 97 2310 1545 2229
Fragments arranged by size:
2310 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2631 1348
Size: 1283 4898
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2690 1108
Size: 1582 4599
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3022 3086 3022
Size: 64 6117
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PstI C_TGCA'G
Cuts at: 830 2886 2954 3281 3439 3533 830
Size: 2056 68 327 158 94 3478
Fragments arranged by size:
3478 2056 327 158 94 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2273 2587 2881 2273
Size: 314 294 5573
Fragments arranged by size:
5573 314 294
SacI G_AGCT'C
Cuts at: 2122 2579 2970 2122
Size: 457 391 5333
Fragments arranged by size:
5333 457 391
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SanDI GG'GwC_CC
Cuts at: 3022 3022
Size: 6181
SapI GCTCTTCn'nnn_
Cuts at: 2469 2469
Size: 6181
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SfcI C'TryA_G
Cuts at: 826 1045 2107 2882 2950 3277 3435 3529 4664
Size: 219 1062 775 68 327 158 94 1135
Cuts at: 4664 5345 5536 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1062 775 681 327 219 191
158 94 68
SfoI GGC'GCC
Cuts at: 2695 2695
Size: 6181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3508 1872
Size: 1636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2121 4285 5186 5448 820
Size: 197 926 178 2164 901 262 1553
Fragments arranged by size:
2164 1553 926 901 262 197 178
SnaBI TAC'GTA
Cuts at: 493 3478 3972 493
Size: 2985 494 2702
Fragments arranged by size:
2985 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6181
StyI C'CwwG_G
Cuts at: 513 1065 2022 2387 3620 513
Size: 552 957 365 1233 3074
Fragments arranged by size:
3074 1233 957 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 2353 4208 4225 4378 4563 5008
Size: 1255 762 11 1855 17 153 185 445
Cuts at: 5008 325
Size: 1498
Fragments arranged by size:
1855 1498 1255 762 445 185 153 17
11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4427 6139 96
Size: 274 80 33 51 1443 2450 1712 138
Fragments arranged by size:
2450 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 6141 6173 2897
Size: 32 3212 32 2905
Fragments arranged by size:
3212 2905 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2998 3328 2998
Size: 330 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3506 1870
Size: 1636 4545
XmnI GAAnn'nnTTC
Cuts at: 4310 4310
Size: 6181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI