Restriction map for
pFN21ASDA1724
(Circular) (Six-base) MAPSORT of: pFN21ASDA1724.seq Check: 610 from: 1 to: 5887
pFN21ASDA1724 5887 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 2840 3065 3230 1937
Size: 673 230 225 165 4594
Fragments arranged by size:
4594 673 230 225 165
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2850 3950 5334 5602 1632
Size: 1218 1100 1384 268 1917
Fragments arranged by size:
1917 1384 1218 1100 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2933 4786 5763 1203
Size: 1730 1853 977 1327
Fragments arranged by size:
1853 1730 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3630 5202 3630
Size: 1572 4315
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 3196 3422 3574 5683 1493
Size: 1703 226 152 2109 1697
Fragments arranged by size:
2109 1703 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
AvrII C'CTAG_G
Cuts at: 3028 3028
Size: 5887
BamHI G'GATC_C
Cuts at: 2850 3217 3584 2850
Size: 367 367 5153
Fragments arranged by size:
5153 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 3208 4663 618
Size: 325 217 627 380 1041 1455 1842
Fragments arranged by size:
1842 1455 1041 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2048 3206 1391
Size: 657 1158 4072
Fragments arranged by size:
4072 1158 657
BbeI G_GCGC'C
Cuts at: 2171 2171
Size: 5887
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BbvCI CC'TCA_GC
Cuts at: 2254 2254
Size: 5887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2086 3779 4989 2086
Size: 1693 1210 2984
Fragments arranged by size:
2984 1693 1210
BclI T'GATC_A
Cuts at: 1548 2395 1548
Size: 847 5040
BfrBI ATG'CAT
Cuts at: 2122 2122
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3128 3304 3128
Size: 176 5711
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 2545 3618
Size: 416 53 83 399 24 876 252 1073
Cuts at: 3618 4576 442
Size: 958 1753
Fragments arranged by size:
1753 1073 958 876 416 399 252 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4547 1239
Size: 3308 2579
Bpu10I CC'TnA_GC
Cuts at: 2254 2254
Size: 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2673 4550 882
Size: 617 1174 1877 2219
Fragments arranged by size:
2219 1877 1174 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 2275 3184 3678 493
Size: 711 837 234 909 494 2702
Fragments arranged by size:
2702 909 837 711 494 234
BsaBI GATnn'nnATC
Cuts at: 2097 5881 2097
Size: 3784 2103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 3694 4076 4711
Size: 53 83 186 936 635 1526 382 635
Cuts at: 4711 275
Size: 1451
Fragments arranged by size:
1526 1451 936 635 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3099 3129 1178
Size: 30 165 30 1696 30 3936
Fragments arranged by size:
3936 1696 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5887
BseYI C'CCAG_C
Cuts at: 5090 5586 5090
Size: 496 5391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3054 3398 3491 3054
Size: 344 93 5450
Fragments arranged by size:
5450 344 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 3206 3948 4033 5104 1391
Size: 399 258 1158 742 85 1071 2174
Fragments arranged by size:
2174 1158 1071 742 399 258 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 4382 4556 65
Size: 1183 1452 1682 174 1396
Fragments arranged by size:
1682 1452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5887
BstBI TT'CG_AA
Cuts at: 2007 4691 2007
Size: 2684 3203
BstEII G'GTnAC_C
Cuts at: 1184 2859 1184
Size: 1675 4212
BstYI r'GATC_y
Cuts at: 1972 2850 3217 3584 3969 3986 5438 5524 5536
Size: 878 367 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 878 385 367 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3066 3066
Size: 5887
Bsu36I CC'TnA_GG
Cuts at: 2380 2380
Size: 5887
BtgI C'CryG_G
Cuts at: 513 1065 2022 2570 513
Size: 552 957 548 3830
Fragments arranged by size:
3830 957 552 548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2688 3403 4197 4217 2688
Size: 715 794 20 4358
Fragments arranged by size:
4358 794 715 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852
Size: 54 1425 50 275 18 1504 889 1629
Cuts at: 5852 8
Size: 43
Fragments arranged by size:
1629 1504 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2452 2730 3008 3817 1353
Size: 1099 278 278 809 3423
Fragments arranged by size:
3423 1099 809 278 278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2850 3950 4547 5334 5602 1239
Size: 393 1218 1100 597 787 268 1524
Fragments arranged by size:
1524 1218 1100 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2171 5034 1678
Size: 493 2863 2531
Fragments arranged by size:
2863 2531 493
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 4528 4560 4602
Size: 32 135 32 827 32 2097 32 42
Cuts at: 4602 4634 1373
Size: 32 2626
Fragments arranged by size:
2626 2097 827 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KasI G'GCGC_C
Cuts at: 2167 2167
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2890 2999 5001 5185 1305
Size: 1585 109 2002 184 2007
Fragments arranged by size:
2007 2002 1585 184 109
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4065
Fragments arranged by size:
4065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 3845 4204 4363 518
Size: 648 1527 1152 359 159 2042
Fragments arranged by size:
2042 1527 1152 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2941 3127 3303 3980 5128 5373 1606
Size: 450 885 186 176 677 1148 245 2120
Fragments arranged by size:
2120 1148 885 677 450 245 186 176
NarI GG'CG_CC
Cuts at: 2168 2168
Size: 5887
NcoI C'CATG_G
Cuts at: 513 1065 2022 2570 513
Size: 552 957 548 3830
Fragments arranged by size:
3830 957 552 548
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NsiI A_TGCA'T
Cuts at: 2124 2124
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2937 3245 4790 1132
Size: 97 1708 308 1545 2229
Fragments arranged by size:
2229 1708 1545 308 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 2933 4786 2933
Size: 1853 4034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PmlI CAC'GTG
Cuts at: 2275 2275
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 2626 3239 830
Size: 1796 613 3478
Fragments arranged by size:
3478 1796 613
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2056 2941 3127 2056
Size: 885 186 4816
Fragments arranged by size:
4816 885 186
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SexAI A'CCwGG_T
Cuts at: 2260 2260
Size: 5887
SfcI C'TryA_G
Cuts at: 826 1045 2139 2622 2841 3235 4370 5051 5242
Size: 219 1094 483 219 394 1135 681 191
Cuts at: 5242 826
Size: 1471
Fragments arranged by size:
1471 1135 1094 681 483 394 219 219
191
SfoI GGC'GCC
Cuts at: 2169 2169
Size: 5887
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3991 4892 5154 820
Size: 197 926 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3184 3678 493
Size: 2691 494 2702
Fragments arranged by size:
2702 2691 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2642 1517
Size: 1125 4762
StyI C'CwwG_G
Cuts at: 513 1065 2022 2570 3028 3326 513
Size: 552 957 548 458 298 3074
Fragments arranged by size:
3074 957 552 548 458 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3914 3931 4084 4269 4714 325
Size: 1255 2334 17 153 185 445 1498
Fragments arranged by size:
2334 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2079 2930
Size: 274 80 33 51 1443 96 6 851
Cuts at: 2930 4133 5845 96
Size: 1203 1712 138
Fragments arranged by size:
1712 1443 1203 851 274 138 96 80
51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 5847 5879 3099
Size: 32 2716 32 3107
Fragments arranged by size:
3107 2716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 2329 3223 2329
Size: 894 4993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2982 3101 2982
Size: 119 5768
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2365 4016 2365
Size: 1651 4236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BplI BsiWI
BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI