Restriction map for
pFN21ASDA1723
(Circular) (Six-base) MAPSORT of: pFN21ASDA1723.seq Check: 4892 from: 1 to: 9313
pFN21ASDA1723 9313 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2777 2777
Size: 9313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7123 278
Size: 53 83 186 936 5587 2468
Fragments arranged by size:
5587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6204 6656 1937
Size: 4267 452 4594
Fragments arranged by size:
4594 4267 452
Acc65I G'GTAC_C
Cuts at: 6634 6634
Size: 9313
AclI AA'CG_TT
Cuts at: 1563 7440 7813 1563
Size: 5877 373 3063
Fragments arranged by size:
5877 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3952 3958 4064 4166 4473 4631 5344 5492
Size: 2320 6 106 102 307 158 713 148
Cuts at: 5492 5759 5948 6550 6561 7376 8760 9028 1632
Size: 267 189 602 11 815 1384 268 1917
Fragments arranged by size:
2320 1917 1384 815 713 602 307 268
267 189 158 148 106 102 11 6
AflII C'TTAA_G
Cuts at: 820 1017 2617 6484 820
Size: 197 1600 3867 3649
Fragments arranged by size:
3867 3649 1600 197
AflIII A'CryG_T
Cuts at: 1203 2059 6468 8212 9189 1203
Size: 856 4409 1744 977 1327
Fragments arranged by size:
4409 1744 1327 977 856
AgeI A'CCGG_T
Cuts at: 8144 8144
Size: 9313
AhdI GACnn_n'nnGTC
Cuts at: 3274 4017 8042 3274
Size: 743 4025 4545
Fragments arranged by size:
4545 4025 743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 9281
AlwNI CAG_nnn'CTG
Cuts at: 3212 5395 5569 5632 7056 8628 3212
Size: 2183 174 63 1424 1572 3897
Fragments arranged by size:
3897 2183 1572 1424 174 63
ApaI G_GGCC'C
Cuts at: 4113 4113
Size: 9313
ApaLI G'TGCA_C
Cuts at: 7370 8526 7370
Size: 1156 8157
ApoI r'AATT_y
Cuts at: 1493 3256 5749 6231 6252 6622 6848 7000 9109
Size: 1763 2493 482 21 370 226 152 2109
Cuts at: 9109 1493
Size: 1697
Fragments arranged by size:
2493 2109 1763 1697 482 370 226 152
21
AseI AT'TA_AT
Cuts at: 160 1032 7867 160
Size: 872 6835 1606
Fragments arranged by size:
6835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9313
AvaI C'yCGr_G
Cuts at: 1870 1943 4510 4733 5882 5951 6294 6638 1870
Size: 73 2567 223 1149 69 343 344 4545
Fragments arranged by size:
4545 2567 1149 344 343 223 73 69
BamHI G'GATC_C
Cuts at: 6643 7010 6643
Size: 367 8946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5013 5414 5955 6280 6634
Size: 325 217 627 3226 401 541 325 354
Cuts at: 6634 8089 618
Size: 1455 1842
Fragments arranged by size:
3226 1842 1455 627 541 401 354 325
325 217
BanII G_rGCy'C
Cuts at: 1391 4058 4113 4676 4953 5045 5358 6632 1391
Size: 2667 55 563 277 92 313 1274 4072
Fragments arranged by size:
4072 2667 1274 563 313 277 92 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3255 3789 928
Size: 971 1356 534 6452
Fragments arranged by size:
6452 1356 971 534
BbvCI CC'TCA_GC
Cuts at: 3140 3776 3812 3140
Size: 636 36 8641
Fragments arranged by size:
8641 636 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3842 3876 3980 4014 5465 5499 7504
Size: 34 1905 34 104 34 1451 34 2005
Cuts at: 7504 7538 1903
Size: 34 3678
Fragments arranged by size:
3678 2005 1905 1451 104 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2650 7205 8415 2650
Size: 4555 1210 3548
Fragments arranged by size:
4555 3548 1210
BclI T'GATC_A
Cuts at: 1548 3118 4098 1548
Size: 1570 980 6763
Fragments arranged by size:
6763 1570 980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3512 3759 5582
Size: 107 122 71 631 605 1840 247 1823
Cuts at: 5582 7924 136
Size: 2342 1525
Fragments arranged by size:
2342 1840 1823 1525 631 605 247 122
107 71
BglII A'GATC_T
Cuts at: 4167 5488 5538 9308 4167
Size: 1321 50 3770 4172
Fragments arranged by size:
4172 3770 1321 50
BlpI GC'TnA_GC
Cuts at: 2718 5707 6078 6730 2718
Size: 2989 371 652 5301
Fragments arranged by size:
5301 2989 652 371
Bme1580I G_kGCm'C
Cuts at: 1790 4113 5199 5556 5958 6352 7374 8530 1790
Size: 2323 1086 357 402 394 1022 1156 2573
Fragments arranged by size:
2573 2323 1156 1086 1022 402 394 357
BmgBI CAC'GTC
Cuts at: 1318 5458 1318
Size: 4140 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3415 7044 8002
Size: 416 53 83 399 24 1998 3629 958
Cuts at: 8002 442
Size: 1753
Fragments arranged by size:
3629 1998 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5770 5770
Size: 9313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2615 2924 3285 3886 4555 4672 4733 5171
Size: 1376 309 361 601 669 117 61 438
Cuts at: 5171 5981 6340 7973 1239
Size: 810 359 1633 2579
Fragments arranged by size:
2579 1633 1376 810 669 601 438 361
359 309 117 61
Bpu10I CC'TnA_GC
Cuts at: 3140 3776 3812 4649 5496 5645 3140
Size: 636 36 837 847 149 6808
Fragments arranged by size:
6808 847 837 636 149 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4034 5817 7438 8303 8601 4034
Size: 1783 1621 865 298 4746
Fragments arranged by size:
4746 1783 1621 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4259 4846 7976 882
Size: 617 1485 1275 587 3130 2219
Fragments arranged by size:
3130 2219 1485 1275 617 587
BsaAI yAC'GTr
Cuts at: 493 1204 4759 6610 7104 493
Size: 711 3555 1851 494 2702
Fragments arranged by size:
3555 2702 1851 711 494
BsaBI GATnn'nnATC
Cuts at: 6375 9307 6375
Size: 2932 6381
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 7120 7502 8137
Size: 53 83 186 936 1993 3594 382 635
Cuts at: 8137 275
Size: 1451
Fragments arranged by size:
3594 1993 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4942 5238 5328 7746 8144 8418 8565 8976 4942
Size: 296 90 2418 398 274 147 411 5279
Fragments arranged by size:
5279 2418 411 398 296 274 147 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4303 4333 5979 6009 1178
Size: 30 165 30 2900 30 1646 30 4482
Fragments arranged by size:
4482 2900 1646 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3142 3889 4151 4231 1186
Size: 1956 747 262 80 6268
Fragments arranged by size:
6268 1956 747 262 80
BseYI C'CCAG_C
Cuts at: 2049 3749 3833 3848 4090 4289 4904 5187 6048
Size: 1700 84 15 242 199 615 283 861
Cuts at: 6048 8516 9012 2049
Size: 2468 496 2350
Fragments arranged by size:
2468 2350 1700 861 615 496 283 242
199 84 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5294 6493 6555 1163
Size: 573 3558 1199 62 3921
Fragments arranged by size:
3921 3558 1199 573 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 6379 6763 7524 8552 1540
Size: 275 186 2893 1485 384 761 1028 2301
Fragments arranged by size:
2893 2301 1485 1028 761 384 275 186
BsiHKAI G_wGCw'C
Cuts at: 4058 5045 6414 7374 7459 8530 4058
Size: 987 1369 960 85 1071 4841
Fragments arranged by size:
4841 1369 1071 987 960 85
BsmI GAATG_Cn'
Cuts at: 6824 6917 6824
Size: 93 9220
BsmBI CGTCTCn'nnnn_
Cuts at: 4609 4609
Size: 9313
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4058 4113 4676 4953 5045 5199 5358
Size: 399 2268 55 563 277 92 154 159
Cuts at: 5358 5556 5958 6352 6414 6632 7374 7459 8530
Size: 198 402 394 62 218 742 85 1071
Cuts at: 8530 1391
Size: 2174
Fragments arranged by size:
2268 2174 1071 742 563 402 399 394
277 218 198 159 154 92 85 62
55
BspEI T'CCGG_A
Cuts at: 5328 8976 5328
Size: 3648 5665
BspHI T'CATG_A
Cuts at: 5205 7202 8932 5205
Size: 1997 1730 5586
Fragments arranged by size:
5586 1997 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 3186 5118 6668 844
Size: 1933 409 1932 1550 3489
Fragments arranged by size:
3489 1933 1932 1550 409
BsrBI CCG'CTC
Cuts at: 1394 1823 7200 1394
Size: 429 5377 3507
Fragments arranged by size:
5377 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2611 2779 3925 7808 7982 65
Size: 1183 1363 168 1146 3883 174 1396
Fragments arranged by size:
3883 1396 1363 1183 1146 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 7957 8144 1700
Size: 110 6147 187 2869
Fragments arranged by size:
6147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9313
BssSI C'ACGA_G
Cuts at: 3249 7373 8385 3249
Size: 4124 1012 4177
Fragments arranged by size:
4177 4124 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5317 5632 794
Size: 4523 315 4475
Fragments arranged by size:
4523 4475 315
BstBI TT'CG_AA
Cuts at: 2007 8117 2007
Size: 6110 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9313
BstXI CCAn_nnnn'nTGG
Cuts at: 3910 4559 4912 3910
Size: 649 353 8311
Fragments arranged by size:
8311 649 353
BstYI r'GATC_y
Cuts at: 1972 3209 4167 4708 4959 5488 5538 6552 6643
Size: 1237 958 541 251 529 50 1014 91
Cuts at: 6643 7010 7395 7412 8864 8950 8962 9308 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1237 1014 958 541 529 385
367 346 251 91 86 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 4574 4574
Size: 9313
BtgI C'CryG_G
Cuts at: 513 1065 3084 3279 4203 4552 513
Size: 552 2019 195 924 349 5274
Fragments arranged by size:
5274 2019 924 552 349 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5664 487
Size: 683 56 4438 4136
Fragments arranged by size:
4438 4136 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 2658 2978 6829 7623 7643 2471
Size: 187 320 3851 794 20 4141
Fragments arranged by size:
4141 3851 794 320 187 20
ClaI AT'CG_AT
Cuts at: 1554 3459 1554
Size: 1905 7408
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2256 2291 529
Size: 35 1692 35 7551
Fragments arranged by size:
7551 1692 35 35
DraI TTT'AAA
Cuts at: 2406 3540 6022 6043 6617 2406
Size: 1134 2482 21 574 5102
Fragments arranged by size:
5102 2482 1134 574 21
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8320 809
Size: 507 7004 1802
Fragments arranged by size:
7004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2997 4561 5051
Size: 54 1425 50 275 18 1167 1564 490
Cuts at: 5051 5697 6760 7649 9278 8
Size: 646 1063 889 1629 43
Fragments arranged by size:
1629 1564 1425 1167 1063 889 646 490
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6760 1537
Size: 275 4948 4090
Fragments arranged by size:
4948 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2903 3669 4607 4941 5204 5363 5726 5735
Size: 1550 766 938 334 263 159 363 9
Cuts at: 5735 7243 1353
Size: 1508 3423
Fragments arranged by size:
3423 1550 1508 938 766 363 334 263
159 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4721 7900 8274 8420 387
Size: 1064 138 2573 559 3179 374 146 1280
Fragments arranged by size:
3179 2573 1280 1064 559 374 146 138
EcoICRI GAG'CTC
Cuts at: 4056 5043 4056
Size: 987 8326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 2924 3285 3886 3952 3958 4064
Size: 393 983 309 361 601 66 6 106
Cuts at: 4064 4166 4473 4555 4631 4672 4733 5171 5344
Size: 102 307 82 76 41 61 438 173
Cuts at: 5344 5492 5759 5948 5981 6340 6550 6561 7376
Size: 148 267 189 33 359 210 11 815
Cuts at: 7376 7973 8760 9028 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 983 815 787 601 597 438 393
361 359 309 307 268 267 210 189
173 148 106 102 82 76 66 61
41 33 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3737 5758 3737
Size: 2021 7292
EcoO109I rG'GnC_Cy
Cuts at: 3717 4305 4699 5254 6107 3717
Size: 588 394 555 853 6923
Fragments arranged by size:
6923 853 588 555 394
EcoRI G'AATT_C
Cuts at: 5749 6622 7000 5749
Size: 873 378 8062
Fragments arranged by size:
8062 873 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3047 3047
Size: 9313
FspI TGC'GCA
Cuts at: 7819 7819
Size: 9313
HaeII r_GCGC'y
Cuts at: 1678 3787 4082 5569 5714 6175 8460 1678
Size: 2109 295 1487 145 461 2285 2531
Fragments arranged by size:
2531 2285 2109 1487 461 295 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3188 3220 3260 3275 3292
Size: 32 135 32 1616 32 40 15 17
Cuts at: 3292 3307 4003 4035 4045 4077 4768 4800 4808
Size: 15 696 32 10 32 691 32 8
Cuts at: 4808 4826 4840 4858 5032 5064 5377 5409 5884
Size: 18 14 18 174 32 313 32 475
Cuts at: 5884 5916 6584 6616 7954 7986 8028 8060 1373
Size: 32 668 32 1338 32 42 32 2626
Fragments arranged by size:
2626 1616 1338 696 691 668 475 313
174 135 42 40 32 32 32 32
32 32 32 32 32 32 32 32
18 18 17 15 15 14 10 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4377 5269 5431 6481 6657 6903
Size: 988 281 2439 892 162 1050 176 246
Cuts at: 6903 669
Size: 3079
Fragments arranged by size:
3079 2439 1050 988 892 281 246 176
162
HindIII A'AGCT_T
Cuts at: 748 2614 3387 4546 5867 748
Size: 1866 773 1159 1321 4194
Fragments arranged by size:
4194 1866 1321 1159 773
HpaI GTT'AAC
Cuts at: 6903 6903
Size: 9313
KpnI G_GTAC'C
Cuts at: 6638 6638
Size: 9313
MfeI C'AATT_G
Cuts at: 2693 6912 2693
Size: 4219 5094
MluI A'CGCG_T
Cuts at: 9189 9189
Size: 9313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4242 4251 4773 5031 6192 8427 8611 1305
Size: 2937 9 522 258 1161 2235 184 2007
Fragments arranged by size:
2937 2235 2007 1161 522 258 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2999 4563 10
Size: 54 1425 343 1167 1564 4760
Fragments arranged by size:
4760 1564 1425 1167 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 3767 4878 6467 6531 7271 7630
Size: 648 1132 1469 1111 1589 64 740 359
Cuts at: 7630 7789 518
Size: 159 2042
Fragments arranged by size:
2042 1589 1469 1132 1111 740 648 359
159 64
MspA1I CmG'CkG
Cuts at: 1606 2891 4107 4774 5978 6539 6729 7406 8554
Size: 1285 1216 667 1204 561 190 677 1148
Cuts at: 8554 8799 1606
Size: 245 2120
Fragments arranged by size:
2120 1285 1216 1204 1148 677 667 561
245 190
NcoI C'CATG_G
Cuts at: 513 1065 3084 4203 513
Size: 552 2019 1119 5623
Fragments arranged by size:
5623 2019 1119 552
NdeI CA'TA_TG
Cuts at: 387 2439 5847 387
Size: 2052 3408 3853
Fragments arranged by size:
3853 3408 2052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9313
NotI GC'GGCC_GC
Cuts at: 6760 6760
Size: 9313
NspI r_CATG'y
Cuts at: 1132 1229 2063 2115 2351 2971 4345 5470 6472
Size: 97 834 52 236 620 1374 1125 1002
Cuts at: 6472 6499 6671 8216 1132
Size: 27 172 1545 2229
Fragments arranged by size:
2229 1545 1374 1125 1002 834 620 236
172 97 52 27
PasI CC'CwG_GG
Cuts at: 1348 3713 5760 1348
Size: 2365 2047 4901
Fragments arranged by size:
4901 2365 2047
PciI A'CATG_T
Cuts at: 2059 6468 8212 2059
Size: 4409 1744 3160
Fragments arranged by size:
4409 3160 1744
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2074 5095 5341 1108
Size: 966 3021 246 5080
Fragments arranged by size:
5080 3021 966 246
PfoI T'CCnGG_A
Cuts at: 4962 5381 5997 6555 4962
Size: 419 616 558 7720
Fragments arranged by size:
7720 616 558 419
PmeI GTTT'AAAC
Cuts at: 6617 6617
Size: 9313
PmlI CAC'GTG
Cuts at: 4759 4759
Size: 9313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5504 5536 6177 6209 7382 7414 8895 8927 5504
Size: 32 641 32 1173 32 1481 32 5890
Fragments arranged by size:
5890 1481 1173 641 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3717 4305 4699 5254 6107 3717
Size: 588 394 555 853 6923
Fragments arranged by size:
6923 853 588 555 394
PshAI GACnn'nnGTC
Cuts at: 1613 2214 1613
Size: 601 8712
PsiI TTA'TAA
Cuts at: 6883 6883
Size: 9313
PspOMI G'GGCC_C
Cuts at: 4109 4109
Size: 9313
PstI C_TGCA'G
Cuts at: 830 2862 5118 5574 5628 6665 830
Size: 2032 2256 456 54 1037 3478
Fragments arranged by size:
3478 2256 2032 1037 456 54
PvuI CG_AT'CG
Cuts at: 2001 6379 2001
Size: 4378 4935
PvuII CAG'CTG
Cuts at: 2891 5978 6539 2891
Size: 3087 561 5665
Fragments arranged by size:
5665 3087 561
SacI G_AGCT'C
Cuts at: 4058 5045 4058
Size: 987 8326
SalI G'TCGA_C
Cuts at: 1936 6655 1936
Size: 4719 4594
SanDI GG'GwC_CC
Cuts at: 3717 5254 3717
Size: 1537 7776
SapI GCTCTTCn'nnn_
Cuts at: 4941 5363 4941
Size: 422 8891
SbfI CC_TGCA'GG
Cuts at: 5574 6665 5574
Size: 1091 8222
ScaI AGT'ACT
Cuts at: 7561 9273 7561
Size: 1712 7601
SexAI A'CCwGG_T
Cuts at: 3705 5000 3705
Size: 1295 8018
SfcI C'TryA_G
Cuts at: 826 1045 2028 2858 5075 5114 5534 5570 5624
Size: 219 983 830 2217 39 420 36 54
Cuts at: 5624 6661 7796 8477 8668 826
Size: 1037 1135 681 191 1471
Fragments arranged by size:
2217 1471 1135 1037 983 830 681 420
219 191 54 39 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9313
SmaI CCC'GGG
Cuts at: 1872 6640 1872
Size: 4768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2617 4049 5832 6294 6484 7417
Size: 197 926 674 1432 1783 462 190 933
Cuts at: 7417 8318 8580 820
Size: 901 262 1553
Fragments arranged by size:
1783 1553 1432 933 926 901 674 462
262 197 190
SnaBI TAC'GTA
Cuts at: 493 6610 7104 493
Size: 6117 494 2702
Fragments arranged by size:
6117 2702 494
SpeI A'CTAG_T
Cuts at: 152 2125 2341 152
Size: 1973 216 7124
Fragments arranged by size:
7124 1973 216
SphI G_CATG'C
Cuts at: 1132 2971 6671 1132
Size: 1839 3700 3774
Fragments arranged by size:
3774 3700 1839
SspI AAT'ATT
Cuts at: 5 52 7237 5
Size: 47 7185 2081
Fragments arranged by size:
7185 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9313
StyI C'CwwG_G
Cuts at: 513 1065 2413 2446 3084 3381 3468 3951 4203
Size: 552 1348 33 638 297 87 483 252
Cuts at: 4203 4602 5556 6752 513
Size: 399 954 1196 3074
Fragments arranged by size:
3074 1348 1196 954 638 552 483 399
297 252 87 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 3017 4828 4908 4918 6383 7340
Size: 1255 1073 364 1811 80 10 1465 957
Cuts at: 7340 7357 7510 7695 8140 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1811 1498 1465 1255 1073 957 445 364
185 153 80 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2779 4344 7559
Size: 274 80 33 51 1443 802 1565 3215
Cuts at: 7559 9271 96
Size: 1712 138
Fragments arranged by size:
3215 1712 1565 1443 802 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3574 3606 4303 4335 9273 9305 3574
Size: 32 697 32 4938 32 3582
Fragments arranged by size:
4938 3582 697 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3707 4838 5426 1316
Size: 2391 1131 588 5203
Fragments arranged by size:
5203 2391 1131 588
XbaI T'CTAG_A
Cuts at: 6649 6649
Size: 9313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5836 5836
Size: 9313
XhoI C'TCGA_G
Cuts at: 1943 6294 1943
Size: 4351 4962
XmaI C'CCGG_G
Cuts at: 1870 6638 1870
Size: 4768 4545
XmnI GAAnn'nnTTC
Cuts at: 4975 5529 5870 7442 4975
Size: 554 341 1572 6846
Fragments arranged by size:
6846 1572 554 341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7121 276
Size: 53 83 186 936 5587 2468
Fragments arranged by size:
5587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI